Difference between revisions of "Team:Wageningen UR/Achievements"

 
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<h1><b>JUDGING CRITERIA</b></h1>
 
<h1><b>JUDGING CRITERIA</b></h1>
  
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<p>In fulfillment of the requirements for the Gold Medal, the 2016 Wageningen iGEM Team did the following:</P>
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<p>In fulfillment of the requirements for the Gold Medal, the 2016 Wageningen iGEM team accomplished the following:</p>
 
<br>
 
<br>
  
 
<h2>Bronze Medal Requirements：</h2>
 
<h2>Bronze Medal Requirements：</h2>
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<img src="https://static.igem.org/mediawiki/2016/6/6a/T--Wageningen_UR--bronze.jpg" align="right" width="100">
 
<ol>
 
<ol>
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<li style="text-align:left">Completed the registration requirements and Project Summary form. Prepared and will present a poster and talk at the 2016 Jamboree.
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<li style="text-align:left"> Registered for iGEM and will present the project at the 2016 Giant Jamboree.
 
</li>
 
</li>
 
<li style="text-align:left">
 
<li style="text-align:left">
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Entered all necessary information detailing 39  <a href="https://2016.igem.org/Team:Wageningen_UR/Parts">BioBricks</a>
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Created and submitted to the Registry 31 new <a href="https://2016.igem.org/Team:Wageningen_UR/Parts">BioBricks</a>
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into the Registry of Standard Parts.
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</li>
 
</li>
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<li style="text-align:left">Designed parts in conformity with accepted BioBrick standards.
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<li style="text-align:left">Created a documented <a href="https://2016.igem.org/Team:Wageningen_UR/Attributions">attributions page</a> in our wiki.
 
</li>
 
</li>
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<li style="text-align:left">
 
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DNA for 39 BioBricks entered in the Registry has been sent in to iGEM headquarters. The majority of these parts were confirmed with DNA sequencing.
 
  
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</li>
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<li style="text-align:left">Completed the Safety and Judging forms and met all other required deliverables.</li>
 
</ol>
 
</ol>
 
<br>
 
<br>
 
<h2>Silver Medal Requirements：</h2>
 
<h2>Silver Medal Requirements：</h2>
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<img src="https://static.igem.org/mediawiki/2016/a/a5/T--Wageningen_UR--silver.jpg" align="right" width="100">
 
<ol>
 
<ol>
  
  
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<li>Demonstrated that several submitted new biobricks work as expected:</li>
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<li>Validated the functionality of several of the submitted BioBricks.</li>
  
 
<ul>
 
<ul>
  
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<li>The composite of chitinase A and B (<a href="http://parts.igem.org/Part:BBa_K1913002">BBa_K1913002, </a><a href="http://parts.igem.org/Part:BBa_K1913003">BBa_K1913003</a>) from <i>S. marcecens</i>. These chitinases have been demonstrated to have relative specific toxicity to V. destructor. we modified these chitinases gene and made them into biobricks. </li>
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<li><a href="http://parts.igem.org/Part:BBa_K1913005">BBa_K1913005</a> encodes a quorum sensing system with GFP as a system output. We characterized this BioBrick, showing its functionality. </li>
  
  
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<li>The composites of Vitamin B12 and Guanine riboswitches (<a href="http://parts.igem.org/Part:BBa_K1913008">BBa_K1913008,</a> <a href="http://parts.igem.org/Part:BBa_K1913009">BBa_K1913009</a>) from <i>E.coli</i> K12 and <i>Bacillus subtilis</i> respectively, which negatively modulate translation upon vitamin B12 and guanine binding. These composites with RFP reporter, was shown to be functional in vivo.</li>
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<li> <a href="http://parts.igem.org/Part:BBa_K1913008">BBa_K1913008,</a> <a href="http://parts.igem.org/Part:BBa_K1913009">BBa_K1913009</a> are a Vitamin B12 and a guanine riboswitch, respectively. Both of them were functionally validated <i>in vivo</i> using RFP as reporter.</li>
  
  
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<li>The composite of cry3Aa toxin (<a href="http://parts.igem.org/Part:BBa_K1913015">BBa_K1913015</a>) from <i>Bacillus thuringiensis</i> Bt886, this gene encodes an 85.78-kDa, cry3Aa1 like protein that has a high level of toxicity towards most kind of insects. We tested its toxicity in vitro by using brush border membrane vesicles (BBMV).</li>
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<li><a href="http://parts.igem.org/Part:BBa_K1913015">BBa_K1913015</a> encodes a Cry3Aa1-like protein that has a high level of toxicity towards most kinds of insects. We experimentally validated its toxicity <i>in vitro</i> by using brush border membrane vesicles (BBMV).</li>
  
  
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<li>FixK2/plac hybrid promoters (<a href="http://parts.igem.org/Part:BBa_K1913023">BBa_K1913023,</a> <a href="http://parts.igem.org/Part:BBa_K1913022">BBa_K1913022</a>) are integrated part of natural FixK2 promoter sequence from <i>B. japonicum</i> with common FixJ binding site and lac operator. It could be either activated by YF1-FixJ generator (<a href="http://parts.igem.org/Part:BBa_K1913034">BBa_K1913034</a>) or inhibit by lacI. </li> </ul>
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<li> <a href="http://parts.igem.org/Part:BBa_K1913025">BBa_K1913025</a> and <a href="http://parts.igem.org/Part:BBa_K1913026">BBa_K1913026</a> are FixK2/plac hybrid promoters, which were functionally validated in combination with a light sensor generator(<a href="http://parts.igem.org/Part:BBa_K1913034">BBa_K1913034</a>).</li></ul>
  
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<li>Considered safety in our project by implementing a optogenetic kill switch system and a synthetic amino acid system to prevent horizontal gene transfer. By implementing these systems, we will reduce the environmental impact of BeeT.</li>
  
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<li>Considered safety in our project by implementing a light Kill-Switch system and a synthetic amino acid system to prevent horizontal gene transfer. By implementing these systems, we will reduce the environmental impact of BT.</li>
 
  
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<li>Collaborated with the TU Delft and Groningen iGEM teams.</li>
  
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<li>Collaborated with Groningen university, Delft university iGEM team to test and characterize new biobricks from each other. Delft iGEM team assisted us to make electron microscopy image of membrane vesicles, we helped them to characterized different fluorescent protein. Also, we helped Groningen iGEM team to sequence their biobricks and they tested our riboswitch device in return.</li>
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<li>As human practice activities, we discussed our project application scenario with Synenergene and we wrote a future scenario involving BeeT application in collaboration with them. We also organized the national Dutch iGEM conference with other iGEM teams to share our experiences. </li> </ol>
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<li>For the human practice part, collaborated with Synenergene and discussed our project application scenario and a techno-moral vignette so that we created a magazine about the future techno moral consequences and social impact on our product. In addition, contacted various Dutch newspaper and magazines to publicize our project and synthetic biology widely and participated in several conferences to inform different stakeholders about our project and collect feedback on our project application. Also, organized the national conference with Dutch iGEM teams to communicate and share own iGEM experience. </li> </ol>
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<br>
 
<br>
  
 
<h2>Gold Medal Requirements:</h2>
 
<h2>Gold Medal Requirements:</h2>
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<img src="https://static.igem.org/mediawiki/2016/6/6a/T--Wageningen_UR--gold.jpg" align="right" width="100">
 
<ol>
 
<ol>
  
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<li>Improve and verify several previous parts:</li>
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<li>Improved and verify several previous parts:</li>
  
 
<ul>
 
<ul>
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<li>
 
<li>
  
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Improved upon the existing set of quorum sensing composite part (<a href="http://parts.igem.org/Part:BBa_K546005">BBa_K546005</a>) by reducing an additional luxR coding sequence and made a better characterization results of this system.</li>
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We improved the characterization of <a href="http://parts.igem.org/Part:BBa_K546000">BBa_K546000</a>, a quorum sensing composite part that we tested together with <a href="http://parts.igem.org/Part:BBa_K1913005">BBa_K1913005</a>, resulting in these <a href="http://parts.igem.org/Part:BBa_K546000:Experience">fluorescence graphs</a>. </li>
  
  
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<li>Designed and provided new FixK2 promoters by adding core control element and typical FixJ binding sites and operons, making them could be controlled by different inputs signal. According to our experimental results, the original FixK2 promoter (<a href="http://parts.igem.org/Part:BBa_K592006">BBa_K592006</a>) in iGEM part registry has lower intensity of transcription compared to our new hybrid FixK2 promoter. Thus, we created a new functional FixK2 promoter.</li>
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<li>Based on the original FixK2 promoter (<a href="http://parts.igem.org/Part:BBa_K592006">BBa_K592006</a> made by Uppsala-Sweden_2011), we designed and provided new <a href="http://parts.igem.org/Part:BBa_K1913025">FixK2 promoters</a> by adding a core control element and operons to the canonical FixJ binding sites, making them controllable by different input signals.</li>  
  
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</ul>
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<li>Verified the existing set of secretion system composite (<a href="http://partsregistry.org/Part:BBa_K215108">BBa_K215108</a>) by re-sequencing the full sequence. </li></ul>
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<p>We consulted and communicated with several stakeholders and specialist such as beekeepers from the Dutch beekeeper association, specialists from the university to obtain their opinions and suggestions in order to make our BT product to be more feasible and acceptable. According to their suggestions of Dutch Beekeeper association, our product should fit with hobbyist character of beekeeping and never affect honey production. Therefore, we amended our project design in the following two aspects. First of all, the existing insecticide-acaricidei are prone to result in resistance of mite if it could not be applied in an appropriate way. In order to prevent development of resistance to our specific anti-mite toxin, we used two kinds of riboswitch and quorum sensing system to make sure our toxin production keep within a reasonable range. In addition, we collaborated with Design Academy Eindhoven to design a tea bag like product that beekeeper can apply it before the beginning of winter to feed bees with sugar water, which could adapt to hobbyist character of beekeeping and avoid interference of honey production. </p>
+
<p>We consulted several stakeholders and specialists such as beekeepers from the Dutch Beekeeper association and researchers from Wageningen University to get suggestions in order to make our BeeT product more feasible and acceptable. According to their suggestions, our product should fit with the hobbyist character of beekeeping and never affect honey production. We amended our project design following their suggestions. In addition, we collaborated with Design Academy Eindhoven to design a tea bag-like product that beekeepers can easily apply. </p>
 +
 
 +
<li>Demonstrate: </li>
 +
<p>We <a href="https://2016.igem.org/Team:Wageningen_UR/Demonstrate">demonstrated</a> some of BeeT's functionalities in conditions which simulate its real-life application. </p>
 +
 
  
  

Latest revision as of 03:18, 20 October 2016

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Wageningen UR iGEM 2016

 

 

 

JUDGING CRITERIA

In fulfillment of the requirements for the Gold Medal, the 2016 Wageningen iGEM team accomplished the following:


Bronze Medal Requirements：

  1. Registered for iGEM and will present the project at the 2016 Giant Jamboree.
  2. Created and submitted to the Registry 31 new BioBricks
  3. Created a documented attributions page in our wiki.
  4. Completed the Safety and Judging forms and met all other required deliverables.

Silver Medal Requirements：

  1. Validated the functionality of several of the submitted BioBricks.
    • BBa_K1913005 encodes a quorum sensing system with GFP as a system output. We characterized this BioBrick, showing its functionality.
    • BBa_K1913008, BBa_K1913009 are a Vitamin B12 and a guanine riboswitch, respectively. Both of them were functionally validated in vivo using RFP as reporter.
    • BBa_K1913015 encodes a Cry3Aa1-like protein that has a high level of toxicity towards most kinds of insects. We experimentally validated its toxicity in vitro by using brush border membrane vesicles (BBMV).
    • BBa_K1913025 and BBa_K1913026 are FixK2/plac hybrid promoters, which were functionally validated in combination with a light sensor generator(BBa_K1913034).
  2. Considered safety in our project by implementing a optogenetic kill switch system and a synthetic amino acid system to prevent horizontal gene transfer. By implementing these systems, we will reduce the environmental impact of BeeT.
  3. Collaborated with the TU Delft and Groningen iGEM teams.
  4. As human practice activities, we discussed our project application scenario with Synenergene and we wrote a future scenario involving BeeT application in collaboration with them. We also organized the national Dutch iGEM conference with other iGEM teams to share our experiences.

Gold Medal Requirements:

  1. Improved and verify several previous parts:
    • We improved the characterization of BBa_K546000, a quorum sensing composite part that we tested together with BBa_K1913005, resulting in these fluorescence graphs.
    • Based on the original FixK2 promoter (BBa_K592006 made by Uppsala-Sweden_2011), we designed and provided new FixK2 promoters by adding a core control element and operons to the canonical FixJ binding sites, making them controllable by different input signals.
  2. Integrated Human Practices:

    3. We consulted several stakeholders and specialists such as beekeepers from the Dutch Beekeeper association and researchers from Wageningen University to get suggestions in order to make our BeeT product more feasible and acceptable. According to their suggestions, our product should fit with the hobbyist character of beekeeping and never affect honey production. We amended our project design following their suggestions. In addition, we collaborated with Design Academy Eindhoven to design a tea bag-like product that beekeepers can easily apply.

  3. Demonstrate:

    4. We demonstrated some of BeeT's functionalities in conditions which simulate its real-life application.