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<html> | <html> | ||
+ | <head> | ||
+ | <!-- Wiki Style Reset (From U of C)--> | ||
+ | <style type="text/css"> | ||
+ | /* Reset the wiki style by overriding with a reset stylesheet. | ||
+ | The relevant wiki css rules are reset by using rules with higher priority. | ||
+ | (Written by Florian Albrecht) | ||
+ | Shoutout to the 2016 U of C wiki */ | ||
+ | /* Start general reset ================================================== */ | ||
+ | html body {font-size: inherit;font-family: inherit;background-color: #b0bec5;color: inherit;padding: 0;} | ||
+ | div#globalWrapper {font-size: inherit;margin: 0;padding: 0;position: static;width: auto;} | ||
+ | /* Start top-section reset ============================================== */ | ||
+ | div#top-section {border: 0;height: auto;margin: 0;position: static;width: auto;} | ||
+ | div#p-logo {height: auto;position: static;width: auto;z-index: auto;} | ||
+ | div#p-logo a, div#p-logo a:hover {text-decoration: none; font-family: 'Roboto', sans-serif;} | ||
+ | div#menubar {font-family: inherit;font-size: inherit;line-height: inherit;position: static;top: 0px;white-space: normal;width: auto;z-index: auto;} | ||
+ | div.left-menu, div.left-menu a {color: inherit;left: 0;text-align: left;text-transform: none;font-family: 'Roboto', sans-serif;} | ||
+ | div#menubar ul {color: inherit;list-style: disc outside none;margin: 0;padding: 0; font-family: 'Roboto', sans-serif;} | ||
+ | div#menubar li {cursor: auto;display: list-item;padding: 0;margin: 0;position: static; font-family: 'Roboto', sans-serif;} | ||
+ | div.left-menu li a {padding: 0; font-family: 'Roboto', sans-serif;} | ||
+ | div.left-menu:hover {color: inherit;background-color: transparent;} | ||
+ | div.left-menu:hover a {color: inherit;font-family: 'Roboto', sans-serif;} | ||
+ | div.right-menu, div.right-menu a {color: inherit;right: 0;text-align: left;font-family: 'Roboto', sans-serif;} | ||
+ | div.right-menu li a {background-color: transparent;color: inherit;padding: 0;font-family: 'Roboto', sans-serif;} | ||
+ | div.right-menu li a:hover {color: inherit;text-decoration: none;font-family: 'Roboto', sans-serif;} | ||
+ | div#search-controls {background-color: transparent;height: auto;position: static;right: 0;text-align: left;top: 0;width: auto;z-index: auto;} | ||
+ | /* Start content reset ================================================== */ | ||
+ | div#content {background: transparent;border: 0;color: inherit;line-height: inherit;margin: 0;padding: 0;position: static;width: auto;z-index: auto;} | ||
+ | a:link, a:hover, a:active, a:visited{color: white; text-decoration: none;font-family: 'Roboto', sans-serif;} | ||
+ | body li {margin: 0; font-family: 'Roboto', sans-serif;} | ||
+ | body h1, body h2, body h3, body h4, body h5, body h6 {background: transparent;border: 0;color: inherit;padding: 0; font-family: 'Roboto', sans-serif;} | ||
+ | body .firstHeading {margin: 0;} | ||
+ | div#contentSub {color: inherit;font-size: inherit;line-height: inherit;margin: 0;width: auto;} | ||
+ | div#catlinks {background-color: transparent;border: 0;clear: both;margin: 0;padding: 0;} | ||
+ | /* Start footer reset =================================================== */ | ||
+ | div#footer-box {background-color: transparent;border: 0;margin: 0;padding: 0;width: auto;} | ||
+ | div#footer {color: inherit;font-size: inherit;text-align: left;} | ||
+ | div#f-poweblue-greybyico, div#f-copyrightico {float: none;} | ||
+ | div#footer li {margin: 0;display: list-item; font-family: 'Roboto', sans-serif;} | ||
+ | </style> | ||
+ | <!-- SideMenu for iGEM Stuff --> | ||
+ | <style type="text/css"> | ||
+ | #sideMenu{width: 200px;position: absolute;top: 20px;left: 1020px;z-index: 10;padding-top: 0px;padding-bottom: 15px;padding-left: 15px;padding-right: 15px;background-color: white;text-align: left;display: none;z-index: 9996;} | ||
+ | #content{width: 100%;padding: 0px;margin-left: 0px;} | ||
+ | #top_title{overflow: hidden;display: none;} | ||
+ | #top_menu_14{height: 20px;} | ||
+ | #top_menu_under{height: 0px;} | ||
+ | #globalWrapper{padding-bottom: 200px;} | ||
+ | </style> | ||
+ | <!-- Custom Styling --> | ||
+ | <style> | ||
+ | a {list-style-image: none;} | ||
+ | ul {list-style-image: none;} | ||
+ | ol {list-style-image: none;} | ||
+ | li {list-style-image: none;} | ||
+ | .waves-effect.waves-blue .waves-ripple { | ||
+ | background-color: rgba(51, 153, 255, 0.65); | ||
+ | } | ||
+ | html { | ||
+ | line-height: 1.5; | ||
+ | font-family: "Roboto" !important, sans-serif; | ||
+ | font-weight: normal; | ||
+ | color: rgba(0, 0, 0, 0.87); | ||
+ | } | ||
+ | #HQ_page h1 {font-family: "Roboto", sans-sefif;} | ||
+ | #HQ_page h2 {font-family: "Roboto", sans-sefif;} | ||
+ | #HQ_page h3 {font-family: "Roboto", sans-sefif;} | ||
+ | #HQ_page h4 {font-family: "Roboto", sans-sefif;} | ||
+ | #HQ_page h5 {font-family: "Roboto", sans-sefif;} | ||
+ | #HQ_page p {font-family: "Roboto", sans-sefif;} | ||
+ | </style> | ||
− | <div | + | <!--More Custom Style for Carousel--> |
+ | <style> /*All of the images linked out to are 400x1920*/ | ||
+ | div.CB1 { | ||
+ | background: url(http://bark-inn.com/wp-content/uploads/2015/11/WATERBALLS-001.jpg) no-repeat center; | ||
+ | width: 100%; | ||
+ | } | ||
+ | div.CB2 { | ||
+ | background: url(https://cdna2.artstation.com/p/assets/images/images/003/290/554/large/adrian-dudak-ny-night.jpg?1472063960) no-repeat center; | ||
+ | width: 100%; | ||
+ | } | ||
+ | div.CB3 { | ||
+ | background: url(http://www.uktoursandvacations.com/media/61605/london-eye-night.jpg?mode=crop&width=1920&height=400¢er=0,0&quality=70&bgcolor=fff) no-repeat center; | ||
+ | width: 100%; | ||
+ | } | ||
+ | div.CB4 { | ||
+ | background: url(http://www.hatfieldswimmingclub.org/wp-content/uploads/2016/02/banner1.jpg) no-repeat center; | ||
+ | width: 100%; | ||
+ | } | ||
+ | </style> | ||
− | < | + | <!-- Import Google Icon Font --> |
+ | <link href="http://fonts.googleapis.com/icon?family=Material+Icons" rel="stylesheet"> | ||
+ | <!-- Roboto Font --> | ||
+ | <link href="https://fonts.googleapis.com/css?family=Roboto" rel="stylesheet"> | ||
+ | <!--Import materialize.css--> | ||
+ | <link type="text/css" rel="stylesheet" href="https://2016.igem.org/Template:Lethbridge/MaterializeCSS?action=raw&ctype=text/css" media="screen,projection"/> | ||
+ | <!--Let browser know website is optimized for mobile--> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"/> | ||
+ | <!-- Page Title --> | ||
+ | <title>Lethbridge iGEM 2016</title> | ||
+ | </head> | ||
− | < | + | <body> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
+ | <div id="content"> | ||
+ | <nav class="blue-grey darken-4" role="navigation" style="margin-top: 15px;"> | ||
+ | <div class="nav-wrapper"> | ||
+ | <a href="https://2016.igem.org/Team:Lethbridge" class="brand-logo left" style="width:auto; left:40px;"> Lethbridge iGEM</a> | ||
+ | <ul class="right hide-on-med-and-down" style="list-style-image: none;"><!--For the top bar--> | ||
+ | <li><a class="dropdown-button" href="#!" data-activates="dropdown1" style="display: block; padding-top: 19px; padding-bottom: 19px">Project</a></li> | ||
+ | <ul id='dropdown1' class="dropdown-content" style="list-style-image: none; margin-left: 0px; width: auto;"> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Description" style="color:#616161;">Description</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Design" style="color:#616161;">Design</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments" style="color:#616161;">Experiment</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Database" style="color:#616161;">Database</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook" style="color:#616161;">Notebook</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Parts" style="color:#616161;">Parts</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Safety" style="color:#616161;">Safety</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/RNAi" style="color:#616161;">RNAi</a></li> | ||
+ | </ul> | ||
+ | <li><a class="dropdown-button" href="#!" data-activates="dropdown2" style="display: block; padding-top: 19px; padding-bottom: 19px">Practices</a></li> | ||
+ | <ul id='dropdown2' class='dropdown-content' style="list-style-image: none; margin-left: 0px; width: auto;"> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices" style="color:#616161;">Integrated Practices</a></li> | ||
+ | <li class="divider"></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#intro" style="color:#78909c;">Contact</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#interviews" style="color:#78909c;">Interviews</a></li><!--NEEDS STUFF--> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#litrev" style="color:#78909c;">Literature Review</a></li> | ||
+ | <li class="divider"></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#ridealong" style="color:#ff8f00;">Ridealongs</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#protocol" style="color:#ff8f00;">Protocol</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#sampling" style="color:#ff8f00;">Sampling</a></li> | ||
+ | </ul> | ||
+ | <li><a class="dropdown-button" href="#!" data-activates="dropdown4" style="display: block; padding-top: 19px; padding-bottom: 19px">Results</a></li> | ||
+ | <ul id='dropdown4' class="dropdown-content" style="list-style-image: none; margin-left: 0px; width: auto;"> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#one" style="color:#616161;">Microbiome</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#two" style="color:#616161;">Antibodies</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#three" style="color:#616161;">RNAiCare</a></li> | ||
+ | </ul> | ||
+ | <li><a class="dropdown-button" href="#!" data-activates="dropdown3" style="display: block; padding-top: 19px; padding-bottom: 19px">Team</a></li> | ||
+ | <ul id='dropdown3' class="dropdown-content" style="list-style-image: none; margin-left: 0px; width: auto;"> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Team" style="color:#616161;">Members</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations" style="color:#616161;">Collab</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions" style="color:#616161;">Attribution</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors" style="color:#616161;">Sponsors</a></li> | ||
+ | </ul> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Achievements" style="display: block; padding-top: 19px; padding-bottom: 19px">Achievements</a></li> | ||
+ | </ul> | ||
− | </div> | + | <a href="#" data-activates="nav-mobile" class="button-collapse"><i class="material-icons">menu</i></a> |
+ | <ul id="nav-mobile" class="side-nav" style="margin-top:20px; margin-left:0px; margin-right:0px; margin-bottom:0px;"> | ||
+ | <li class="no-padding"> | ||
+ | <ul class="collapsible collapsible-accordion"> | ||
+ | <li class="bold"><a class="collapsible-header waves-effect waves-blue">Project</a> | ||
+ | <div class="collapsible-body"> | ||
+ | <ul> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Description">Description</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Design">Design</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments">Experiments</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Database">Database</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook">Notebook</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Parts">Parts</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Safety">Safety</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/RNAi">RNAi</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
− | <div class=" | + | <li class="no-padding"> |
+ | <ul class="collapsible collapsible-accordion"> | ||
+ | <li class="bold"><a class="collapsible-header waves-effect waves-blue">Practices</a> | ||
+ | <div class="collapsible-body"> | ||
+ | <ul> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices">Integrated Practices</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#intro">Intro</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#interviews">Interviews</a></li><!--NEEDS STUFF--> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#litrev" >Literature Review</a></li><!--NEEDS STUFF--> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#ridealong">Ridealongs</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#protocol">Protocol</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#sampling">Sampling</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="no-padding"> | ||
+ | <ul class="collapsible collapsible-accordion"> | ||
+ | <li class="bold"><a class="collapsible-header waves-effect">Results</a> | ||
+ | <div class="collapsible-body"> | ||
+ | <ul> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#one">Microbiome</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#two">Antibodies</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#three">RNAiCare</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="no-padding"> | ||
+ | <ul class="collapsible collapsible-accordion"> | ||
+ | <li class="bold"><a class="collapsible-header waves-effect">Team</a> | ||
+ | <div class="collapsible-body"> | ||
+ | <ul> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Team">Members</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations">Collaborations</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions">Attribution</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="bold"><a class="collapsible-header waves-effect" href="https://2016.igem.org/Team:Lethbridge/Achievements">Achievements</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </nav> | ||
− | |||
− | < | + | <!-- Wiki Content Begins - Edit Here --> |
− | + | <main> | |
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− | < | + | |
+ | <div class="row"> | ||
+ | <div class="col s12 blue-grey darken-1"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col l12 m12 s12"> | ||
+ | <h2 class="thin white-text">Project Description</h2> | ||
+ | |||
+ | <p class="grey-text text-lighten-4">The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.</p> | ||
+ | <img class="responsive-img" src="https://static.igem.org/mediawiki/2016/c/cc/T--Lethbridge--project_overview.jpg"> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
</div> | </div> | ||
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</div> | </div> | ||
+ | <!-- Scripts --> | ||
− | + | <script src="https://2016.igem.org/Template:Team:Lethbridge/jQuery2-1-1?action=raw&ctype=text/javascript" type="text/javascript"></script> | |
− | + | <script src="https://2016.igem.org/Template:Lethbridge/MaterializeJS?action=raw&ctype=text/javascript" type="text/javascript"></script> | |
− | + | <script src="https://2016.igem.org/Template:Lethbridge/InitJS?action=raw&ctype=text/javascript" type="text/javascript"></script> | |
− | + | </body> | |
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Latest revision as of 03:26, 20 October 2016
Project Description
The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.