Difference between revisions of "Team:Lethbridge/Description"

 
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     <div class="nav-wrapper">
 
       <a href="https://2016.igem.org/Team:Lethbridge" class="brand-logo left" style="width:auto; left:40px;"> Lethbridge iGEM</a>
 
       <a href="https://2016.igem.org/Team:Lethbridge" class="brand-logo left" style="width:auto; left:40px;"> Lethbridge iGEM</a>
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             <li><a href="https://2016.igem.org/Team:Lethbridge/Description" style="color:#616161;">Description</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Description" style="color:#616161;">Description</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Design" style="color:#616161;">Design</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Design" style="color:#616161;">Design</a></li>
−
             <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments" style="color:#616161;">Experiments</a></li>
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             <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments" style="color:#616161;">Experiment</a></li>
−
             <li><a href="https://2016.igem.org/Team:Lethbridge/Results" style="color:#616161;">Results</a></li>
+
             <li><a href="https://2016.igem.org/Team:Lethbridge/Database" style="color:#616161;">Database</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook" style="color:#616161;">Notebook</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook" style="color:#616161;">Notebook</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Parts" style="color:#616161;">Parts</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Parts" style="color:#616161;">Parts</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Safety" style="color:#616161;">Safety</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Safety" style="color:#616161;">Safety</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/RNAi" style="color:#616161;">RNAi</a></li>
 
           </ul>
 
           </ul>
 
         <li><a class="dropdown-button" href="#!" data-activates="dropdown2" style="display: block; padding-top: 19px; padding-bottom: 19px">Practices</a></li>
 
         <li><a class="dropdown-button" href="#!" data-activates="dropdown2" style="display: block; padding-top: 19px; padding-bottom: 19px">Practices</a></li>
 
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            <li><a href="https://2016.igem.org/Team:Lethbridge/Human_Practices" style="color:#616161;">Intro</a></li>
 
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            <li><a href="#!" style="color:#616161;">Literature Review</a></li><!--NEEDS STUFF-->
 
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            <li><a href="#!" style="color:#616161;">Interviews</a></li><!--NEEDS STUFF-->
 
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            <li><a href="https://2016.igem.org/Team:Lethbridge/Ridealong" style="color:#616161;">Ridealongs</a></li>
 
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices" style="color:#616161;">Integrated Practices</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices" style="color:#616161;">Integrated Practices</a></li>
 +
            <li class="divider"></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#intro" style="color:#78909c;">Contact</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#interviews" style="color:#78909c;">Interviews</a></li><!--NEEDS STUFF-->
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#litrev" style="color:#78909c;">Literature Review</a></li>
 +
            <li class="divider"></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#ridealong" style="color:#ff8f00;">Ridealongs</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#protocol" style="color:#ff8f00;">Protocol</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#sampling" style="color:#ff8f00;">Sampling</a></li>
 
         </ul>
 
         </ul>
 +
        <li><a class="dropdown-button" href="#!" data-activates="dropdown4" style="display: block; padding-top: 19px; padding-bottom: 19px">Results</a></li>
 +
          <ul id='dropdown4' class="dropdown-content" style="list-style-image: none; margin-left: 0px; width: auto;">
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Results#one" style="color:#616161;">Microbiome</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Results#two" style="color:#616161;">Antibodies</a></li>
 +
            <li><a href="https://2016.igem.org/Team:Lethbridge/Results#three" style="color:#616161;">RNAiCare</a></li>
 +
          </ul>
 
         <li><a class="dropdown-button" href="#!" data-activates="dropdown3" style="display: block; padding-top: 19px; padding-bottom: 19px">Team</a></li>
 
         <li><a class="dropdown-button" href="#!" data-activates="dropdown3" style="display: block; padding-top: 19px; padding-bottom: 19px">Team</a></li>
 
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             <li><a href="https://2016.igem.org/Team:Lethbridge/Team" style="color:#616161;">Members</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Team" style="color:#616161;">Members</a></li>
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             <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations" style="color:#616161;">Collaboration</a></li>
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             <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations" style="color:#616161;">Collab</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions" style="color:#616161;">Attribution</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions" style="color:#616161;">Attribution</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors" style="color:#616161;">Sponsors</a></li>
 
             <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors" style="color:#616161;">Sponsors</a></li>
 
           </ul>
 
           </ul>
 
         <li><a href="https://2016.igem.org/Team:Lethbridge/Achievements" style="display: block; padding-top: 19px; padding-bottom: 19px">Achievements</a></li>
 
         <li><a href="https://2016.igem.org/Team:Lethbridge/Achievements" style="display: block; padding-top: 19px; padding-bottom: 19px">Achievements</a></li>
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        <li><a href="https://2016.igem.org/Team:Lethbridge/Database" style="display: block; padding-top: 19px; padding-bottom: 19px">Database</a></li>
 
 
       </ul>
 
       </ul>
  
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                       <li><a href="https://2016.igem.org/Team:Lethbridge/Design">Design</a></li>
 
                       <li><a href="https://2016.igem.org/Team:Lethbridge/Design">Design</a></li>
 
                       <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments">Experiments</a></li>
 
                       <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments">Experiments</a></li>
−
                       <li><a href="https://2016.igem.org/Team:Lethbridge/Results">Results</a></li>
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                       <li><a href="https://2016.igem.org/Team:Lethbridge/Database">Database</a></li>
 
                       <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook">Notebook</a></li>
 
                       <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook">Notebook</a></li>
 
                       <li><a href="https://2016.igem.org/Team:Lethbridge/Parts">Parts</a></li>
 
                       <li><a href="https://2016.igem.org/Team:Lethbridge/Parts">Parts</a></li>
 
                       <li><a href="https://2016.igem.org/Team:Lethbridge/Safety">Safety</a></li>
 
                       <li><a href="https://2016.igem.org/Team:Lethbridge/Safety">Safety</a></li>
 +
                      <li><a href="https://2016.igem.org/Team:Lethbridge/RNAi">RNAi</a></li>
 
                     </ul>
 
                     </ul>
 
                   </div>
 
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               <li class="bold"><a class="collapsible-header waves-effect waves-blue">Practices</a>
 
                 <div class="collapsible-body">
 
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                   <ul>
 
                   <ul>
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                    <li><a href="https://2016.igem.org/Team:Lethbridge/Human_Practices">Intro</a></li>
 
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                    <li><a href="">Literature Review</a></li>
 
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                    <li><a href="">Interviews</a></li>
 
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                    <li><a href=https://2016.igem.org/Team:Lethbridge/Ridealong>Ridealongs</a></li>
 
 
                     <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices">Integrated Practices</a></li>
 
                     <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices">Integrated Practices</a></li>
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                    <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#intro">Intro</a></li>
 +
                    <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#interviews">Interviews</a></li><!--NEEDS STUFF-->
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                    <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#litrev" >Literature Review</a></li><!--NEEDS STUFF-->
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                    <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#ridealong">Ridealongs</a></li>
 +
                    <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#protocol">Protocol</a></li>
 +
                    <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#sampling">Sampling</a></li>
 
                   </ul>
 
                   </ul>
 
                 </div>
 
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              <li class="bold"><a class="collapsible-header waves-effect">Results</a>
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                  <ul>
 +
                    <li><a href="https://2016.igem.org/Team:Lethbridge/Results#one">Microbiome</a></li>
 +
                    <li><a href="https://2016.igem.org/Team:Lethbridge/Results#two">Antibodies</a></li>
 +
                    <li><a href="https://2016.igem.org/Team:Lethbridge/Results#three">RNAiCare</a></li>
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                  </ul>
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                 <ul>
 
                 <ul>
 
                   <li><a href="https://2016.igem.org/Team:Lethbridge/Team">Members</a></li>
 
                   <li><a href="https://2016.igem.org/Team:Lethbridge/Team">Members</a></li>
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                   <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations">Collaboration</a></li>
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                   <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations">Collaborations</a></li>
 
                   <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions">Attribution</a></li>
 
                   <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions">Attribution</a></li>
 
                   <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li>
 
                   <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li>
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         <li class="bold"><a class="collapsible-header waves-effect waves-orange" href="https://2016.igem.org/Team:Lethbridge/Achievements">Achievements</a></li>
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         <li class="bold"><a class="collapsible-header waves-effect" href="https://2016.igem.org/Team:Lethbridge/Achievements">Achievements</a></li>
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        <li class="bold"><a class="collapsible-header waves-effect waves-orange" href="https://2016.igem.org/Team:Lethbridge/Database">Database</a></li>
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<!-- Wiki Content Begins - Edit Here -->
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        <h1 class="header center red-text">Nanoresponders</h1>
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          <h5 class="header col s12 light">Single-domain antibody production.</h5>
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          <a href="#" id="download-button" class="btn-large waves-effect waves-light red">Explore</a>
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           <h5 class="white-text">Introduction</h5>
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           <h2 class="thin white-text">Project Description</h2>
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           <p class="grey-text text-lighten-4">The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.</p>
 
           <p class="grey-text text-lighten-4">The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.</p>
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Latest revision as of 03:26, 20 October 2016

Lethbridge iGEM 2016

Project Description

The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.