Chris.isaac (Talk | contribs) |
|||
(10 intermediate revisions by 3 users not shown) | |||
Line 1: | Line 1: | ||
− | |||
− | |||
<html> | <html> | ||
− | |||
<head> | <head> | ||
<!-- Wiki Style Reset (From U of C)--> | <!-- Wiki Style Reset (From U of C)--> | ||
Line 12: | Line 9: | ||
/* Start general reset ================================================== */ | /* Start general reset ================================================== */ | ||
− | html body {font-size: inherit;font-family: inherit;background-color: | + | html body {font-size: inherit;font-family: inherit;background-color: #b0bec5;color: inherit;padding: 0;} |
div#globalWrapper {font-size: inherit;margin: 0;padding: 0;position: static;width: auto;} | div#globalWrapper {font-size: inherit;margin: 0;padding: 0;position: static;width: auto;} | ||
/* Start top-section reset ============================================== */ | /* Start top-section reset ============================================== */ | ||
Line 40: | Line 37: | ||
div#footer-box {background-color: transparent;border: 0;margin: 0;padding: 0;width: auto;} | div#footer-box {background-color: transparent;border: 0;margin: 0;padding: 0;width: auto;} | ||
div#footer {color: inherit;font-size: inherit;text-align: left;} | div#footer {color: inherit;font-size: inherit;text-align: left;} | ||
− | div#f- | + | div#f-poweblue-greybyico, div#f-copyrightico {float: none;} |
div#footer li {margin: 0;display: list-item; font-family: 'Roboto', sans-serif;} | div#footer li {margin: 0;display: list-item; font-family: 'Roboto', sans-serif;} | ||
</style> | </style> | ||
Line 60: | Line 57: | ||
ol {list-style-image: none;} | ol {list-style-image: none;} | ||
li {list-style-image: none;} | li {list-style-image: none;} | ||
+ | .waves-effect.waves-blue .waves-ripple { | ||
+ | background-color: rgba(51, 153, 255, 0.65); | ||
+ | } | ||
+ | html { | ||
+ | line-height: 1.5; | ||
+ | font-family: "Roboto" !important, sans-serif; | ||
+ | font-weight: normal; | ||
+ | color: rgba(0, 0, 0, 0.87); | ||
+ | } | ||
+ | #HQ_page h1 {font-family: "Roboto", sans-sefif;} | ||
+ | #HQ_page h2 {font-family: "Roboto", sans-sefif;} | ||
+ | #HQ_page h3 {font-family: "Roboto", sans-sefif;} | ||
+ | #HQ_page h4 {font-family: "Roboto", sans-sefif;} | ||
+ | #HQ_page h5 {font-family: "Roboto", sans-sefif;} | ||
+ | #HQ_page p {font-family: "Roboto", sans-sefif;} | ||
+ | </style> | ||
+ | |||
+ | <!--More Custom Style for Carousel--> | ||
+ | <style> /*All of the images linked out to are 400x1920*/ | ||
+ | div.CB1 { | ||
+ | background: url(http://bark-inn.com/wp-content/uploads/2015/11/WATERBALLS-001.jpg) no-repeat center; | ||
+ | width: 100%; | ||
+ | } | ||
+ | div.CB2 { | ||
+ | background: url(https://cdna2.artstation.com/p/assets/images/images/003/290/554/large/adrian-dudak-ny-night.jpg?1472063960) no-repeat center; | ||
+ | width: 100%; | ||
+ | } | ||
+ | div.CB3 { | ||
+ | background: url(http://www.uktoursandvacations.com/media/61605/london-eye-night.jpg?mode=crop&width=1920&height=400¢er=0,0&quality=70&bgcolor=fff) no-repeat center; | ||
+ | width: 100%; | ||
+ | } | ||
+ | div.CB4 { | ||
+ | background: url(http://www.hatfieldswimmingclub.org/wp-content/uploads/2016/02/banner1.jpg) no-repeat center; | ||
+ | width: 100%; | ||
+ | } | ||
</style> | </style> | ||
Line 77: | Line 109: | ||
<body> | <body> | ||
− | |||
<div id="content"> | <div id="content"> | ||
− | + | <nav class="blue-grey darken-4" role="navigation" style="margin-top: 15px;"> | |
− | <nav class="blue darken- | + | |
<div class="nav-wrapper"> | <div class="nav-wrapper"> | ||
<a href="https://2016.igem.org/Team:Lethbridge" class="brand-logo left" style="width:auto; left:40px;"> Lethbridge iGEM</a> | <a href="https://2016.igem.org/Team:Lethbridge" class="brand-logo left" style="width:auto; left:40px;"> Lethbridge iGEM</a> | ||
Line 88: | Line 118: | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Description" style="color:#616161;">Description</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Description" style="color:#616161;">Description</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Design" style="color:#616161;">Design</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Design" style="color:#616161;">Design</a></li> | ||
− | <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments" style="color:#616161;"> | + | <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments" style="color:#616161;">Experiment</a></li> |
− | <li><a href="https://2016.igem.org/Team:Lethbridge/ | + | <li><a href="https://2016.igem.org/Team:Lethbridge/Database" style="color:#616161;">Database</a></li> |
<li><a href="https://2016.igem.org/Team:Lethbridge/Notebook" style="color:#616161;">Notebook</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook" style="color:#616161;">Notebook</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Parts" style="color:#616161;">Parts</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Parts" style="color:#616161;">Parts</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Safety" style="color:#616161;">Safety</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Safety" style="color:#616161;">Safety</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/RNAi" style="color:#616161;">RNAi</a></li> | ||
</ul> | </ul> | ||
<li><a class="dropdown-button" href="#!" data-activates="dropdown2" style="display: block; padding-top: 19px; padding-bottom: 19px">Practices</a></li> | <li><a class="dropdown-button" href="#!" data-activates="dropdown2" style="display: block; padding-top: 19px; padding-bottom: 19px">Practices</a></li> | ||
<ul id='dropdown2' class='dropdown-content' style="list-style-image: none; margin-left: 0px; width: auto;"> | <ul id='dropdown2' class='dropdown-content' style="list-style-image: none; margin-left: 0px; width: auto;"> | ||
− | |||
− | |||
− | |||
− | |||
<li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices" style="color:#616161;">Integrated Practices</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices" style="color:#616161;">Integrated Practices</a></li> | ||
+ | <li class="divider"></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#intro" style="color:#78909c;">Contact</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#interviews" style="color:#78909c;">Interviews</a></li><!--NEEDS STUFF--> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#litrev" style="color:#78909c;">Literature Review</a></li> | ||
+ | <li class="divider"></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#ridealong" style="color:#ff8f00;">Ridealongs</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#protocol" style="color:#ff8f00;">Protocol</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#sampling" style="color:#ff8f00;">Sampling</a></li> | ||
</ul> | </ul> | ||
+ | <li><a class="dropdown-button" href="#!" data-activates="dropdown4" style="display: block; padding-top: 19px; padding-bottom: 19px">Results</a></li> | ||
+ | <ul id='dropdown4' class="dropdown-content" style="list-style-image: none; margin-left: 0px; width: auto;"> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#one" style="color:#616161;">Microbiome</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#two" style="color:#616161;">Antibodies</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#three" style="color:#616161;">RNAiCare</a></li> | ||
+ | </ul> | ||
<li><a class="dropdown-button" href="#!" data-activates="dropdown3" style="display: block; padding-top: 19px; padding-bottom: 19px">Team</a></li> | <li><a class="dropdown-button" href="#!" data-activates="dropdown3" style="display: block; padding-top: 19px; padding-bottom: 19px">Team</a></li> | ||
<ul id='dropdown3' class="dropdown-content" style="list-style-image: none; margin-left: 0px; width: auto;"> | <ul id='dropdown3' class="dropdown-content" style="list-style-image: none; margin-left: 0px; width: auto;"> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Team" style="color:#616161;">Members</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Team" style="color:#616161;">Members</a></li> | ||
− | <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations" style="color:#616161;"> | + | <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations" style="color:#616161;">Collab</a></li> |
<li><a href="https://2016.igem.org/Team:Lethbridge/Attributions" style="color:#616161;">Attribution</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions" style="color:#616161;">Attribution</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors" style="color:#616161;">Sponsors</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors" style="color:#616161;">Sponsors</a></li> | ||
</ul> | </ul> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Achievements" style="display: block; padding-top: 19px; padding-bottom: 19px">Achievements</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Achievements" style="display: block; padding-top: 19px; padding-bottom: 19px">Achievements</a></li> | ||
− | |||
</ul> | </ul> | ||
Line 117: | Line 157: | ||
<li class="no-padding"> | <li class="no-padding"> | ||
<ul class="collapsible collapsible-accordion"> | <ul class="collapsible collapsible-accordion"> | ||
− | <li class="bold"><a class="collapsible-header waves-effect waves- | + | <li class="bold"><a class="collapsible-header waves-effect waves-blue">Project</a> |
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
Line 123: | Line 163: | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Design">Design</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Design">Design</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Experiments">Experiments</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments">Experiments</a></li> | ||
− | <li><a href="https://2016.igem.org/Team:Lethbridge/ | + | <li><a href="https://2016.igem.org/Team:Lethbridge/Database">Database</a></li> |
<li><a href="https://2016.igem.org/Team:Lethbridge/Notebook">Notebook</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook">Notebook</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Parts">Parts</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Parts">Parts</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Safety">Safety</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Safety">Safety</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/RNAi">RNAi</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
Line 135: | Line 176: | ||
<li class="no-padding"> | <li class="no-padding"> | ||
<ul class="collapsible collapsible-accordion"> | <ul class="collapsible collapsible-accordion"> | ||
− | <li class="bold"><a class="collapsible-header waves-effect waves- | + | <li class="bold"><a class="collapsible-header waves-effect waves-blue">Practices</a> |
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
− | |||
− | |||
− | |||
− | |||
<li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices">Integrated Practices</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices">Integrated Practices</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#intro">Intro</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#interviews">Interviews</a></li><!--NEEDS STUFF--> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#litrev" >Literature Review</a></li><!--NEEDS STUFF--> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#ridealong">Ridealongs</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#protocol">Protocol</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#sampling">Sampling</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
Line 148: | Line 191: | ||
</ul> | </ul> | ||
</li> | </li> | ||
− | + | <li class="no-padding"> | |
− | + | <ul class="collapsible collapsible-accordion"> | |
+ | <li class="bold"><a class="collapsible-header waves-effect">Results</a> | ||
+ | <div class="collapsible-body"> | ||
+ | <ul> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#one">Microbiome</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#two">Antibodies</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:Lethbridge/Results#three">RNAiCare</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="no-padding"> | ||
<ul class="collapsible collapsible-accordion"> | <ul class="collapsible collapsible-accordion"> | ||
− | <li class="bold"><a class="collapsible-header waves-effect | + | <li class="bold"><a class="collapsible-header waves-effect">Team</a> |
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Team">Members</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Team">Members</a></li> | ||
− | <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations"> | + | <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations">Collaborations</a></li> |
<li><a href="https://2016.igem.org/Team:Lethbridge/Attributions">Attribution</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions">Attribution</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li> | ||
Line 163: | Line 218: | ||
</ul> | </ul> | ||
</li> | </li> | ||
− | <li class="bold"><a class="collapsible-header waves-effect | + | <li class="bold"><a class="collapsible-header waves-effect" href="https://2016.igem.org/Team:Lethbridge/Achievements">Achievements</a></li> |
− | + | ||
</ul> | </ul> | ||
</div> | </div> | ||
</nav> | </nav> | ||
+ | |||
<!-- Wiki Content Begins - Edit Here --> | <!-- Wiki Content Begins - Edit Here --> | ||
− | + | <main> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<div class="row"> | <div class="row"> | ||
− | <div class="col s12 blue"> | + | <div class="col s12 blue-grey darken-1"> |
<div class="container"> | <div class="container"> | ||
<div class="row"> | <div class="row"> | ||
− | <div class="col | + | <div class="col l12 m12 s12"> |
− | < | + | <h2 class="thin white-text">Project Description</h2> |
+ | |||
<p class="grey-text text-lighten-4">The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.</p> | <p class="grey-text text-lighten-4">The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.</p> | ||
+ | <img class="responsive-img" src="https://static.igem.org/mediawiki/2016/c/cc/T--Lethbridge--project_overview.jpg"> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 191: | Line 242: | ||
</div> | </div> | ||
− | + | </main> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | |||
</div> | </div> | ||
<!-- Scripts --> | <!-- Scripts --> |
Latest revision as of 03:26, 20 October 2016
Project Description
The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.
![](https://static.igem.org/mediawiki/2016/c/cc/T--Lethbridge--project_overview.jpg)