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| {{BNU-CHINA/partials/header}} | | {{BNU-CHINA/partials/header}} |
| + | {{BNU-CHINA/partials/nav | ACHIEVEMENTS=focus}} |
| + | {{BNU-CHINA/article/theme | color=#281f1d}} |
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| <html> | | <html> |
− | <div class="header-container">
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− | <nav class="navbar navbar-fixed-top navbar-inverse nav-adjust" role="navigation" style="margin-top: 14px;">
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− | <div class="container-fluid nav-container scrolled-top">
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− | <!--<div id="nav-bg">-->
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− | <div class="navbar-header">
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− | <button type="button" class="navbar-toggle collapsed" data-toggle="collapse"
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− | data-target="#bs-example-navbar-collapse-1" id="navbar-toggle">
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− | <span class="sr-only">Toggle navigation</span>
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− | <span class="icon-bar"></span>
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− | <span class="icon-bar"></span>
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− | <span class="icon-bar"></span>
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− | </button>
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− | <img id="taxolightlogo" src="https://static.igem.org/mediawiki/2016/7/77/T--BNU-China--log.jpg"/>
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− | <a class="navbar-brand" id="navbar-brand" href="#">BNU-China</a>
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− | </div>
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− | <!-- Collect the nav links, forms, and other content for toggling -->
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− | <div class="collapse navbar-collapse navbar-right" id="bs-example-navbar-collapse-1">
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− | <ul class="nav navbar-nav">
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− | <li><a href="./index.html">Home</a></li>
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− | <li class="dropdown">
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− | <a href="./project.html" class="dropdown-toggle" data-toggle="dropdown">Project<span
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− | class="caret"></span></a>
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− | <ul class="dropdown-menu" role="menu">
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− | <li><a href="./project.html">Overview</a></li>
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− | <!--<li class="divider"></li>-->
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− | <li><a href="#">Results</a></li>
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− | <li><a href="#">Improvement</a></li>
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− | </ul>
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− | </li>
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− | <li class="dropdown">
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− | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Modeling<span
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− | class="caret"></span></a>
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− | <ul class="dropdown-menu" role="menu">
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− | <li><a href="https://2016.igem.org/Team:BNU-China/Modeling#">Design</a></li>
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− | <li><a href="https://2016.igem.org/Team:BNU-China/Modeling#">Simulation</a></li>
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− | <li><a href="https://2016.igem.org/Team:BNU-China/Modeling#">Database</a></li>
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− | </ul>
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− | </li>
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− | <li class="dropdown focus">
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− | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Achievements<span
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− | class="caret"></span></a>
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− | <ul class="dropdown-menu" role="menu">
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− | <li><a href="https://2016.igem.org/Team:BNU-China/Achievements">Parts</a></li>
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− | <li><a href="https://2016.igem.org/Team:BNU-China/Achievements">Judging</a></li>
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− | </ul>
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− | </li>
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− | <li><a href="./practices.html">Practices</a></li>
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− | <li><a href="./safety.html">Safety</a></li>
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− | <li class="dropdown">
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− | <a href="#" class="dropdown-toggle" data-toggle="dropdown">Team<span
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− | class="caret" style="background: #000000;"></span></a>
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− | <ul class="dropdown-menu" role="menu">
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− | <li><a href="https://2016.igem.org/Team:BNU-China/Team#">Members</a></li>
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− | <li><a href="https://2016.igem.org/Team:BNU-China/Team#">Attributions</a></li>
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− | <li><a href="https://2016.igem.org/Team:BNU-China/Team#">Collaborations</a></li>
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− | <li><a href="https://2016.igem.org/Team:BNU-China/Team#">Acknowledgement</a></li>
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− | <li><a href="https://2016.igem.org/Team:BNU-China/Team#">Notebook</a></li>
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− | <li><a href="https://2016.igem.org/Team:BNU-China/Team#">Protocol</a></li>
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− | </ul>
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− | </li>
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− | </ul>
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− | </div><!-- /.navbar-collapse -->
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− | <!--</div>-->
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− | </div><!-- /.container-fluid -->
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− | </nav>
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− | </div>
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| <div class="main-container"> | | <div class="main-container"> |
− | <div class="container-fluid page-heading" style="background-image: url(https://static.igem.org/mediawiki/2016/9/96/T--BNU-China--team.jpg);"> | + | <div class="container-fluid page-heading" style="background-image: url(https://static.igem.org/mediawiki/2016/e/e3/T--BNU-China--attribution.jpg);"> |
− | <h3> ACHIEVEMENT </h3> | + | <h3> Achievements</h3> |
| </div> | | </div> |
− | <div style="background-image: url(https://static.igem.org/mediawiki/2016/8/88/T--BNU-China--landingImage2.jpg); background-size: 100%;"> | + | <div> |
− | <div class="container page-story"> | + | <div class="page-story"> |
| <article id="achievement" class="col-lg-10 col-lg-offset-1 col-md-12 col-md-offset-0 col-sm-offset-0 col-sm-12"> | | <article id="achievement" class="col-lg-10 col-lg-offset-1 col-md-12 col-md-offset-0 col-sm-offset-0 col-sm-12"> |
| <header class="page-header"> | | <header class="page-header"> |
− | <h1>Achievement</h1> | + | <h1>Achievements</h1> |
− | <small id="secondary-page-header">Strive for excellence</small>
| + | |
| </header> | | </header> |
− | <h2>Our Parts</h2> | + | <h2 class="text-center">Parts</h2> |
− | <h5 class="text-center" style="color: #278A75;">iGEM 2016 BNU-China Standard Biological Parts</h5> | + | <h4 class="text-center">iGEM 2016 BNU-China Standard Biological Parts</h4> |
− | <table class="table table-bordered table-hover">
| + | |
− | <tbody>
| + | |
− | <tr>
| + | |
− | <th>BioBrick</th>
| + | |
− | <th>Name</th>
| + | |
− | <th>Description</th>
| + | |
− | <th>Length</th>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891000</a></td>
| + | |
− | <td>α-tubulin</td>
| + | |
− | <td>Encodes α-tubulin from human breast cancer cell line Mcf-7</td>
| + | |
− | <td>1356</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891001</a></td>
| + | |
− | <td>β-tubulin</td>
| + | |
− | <td>Encodes β-tubulin from human breast cancer cell line Mcf-7</td>
| + | |
− | <td>1335</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891002</a></td>
| + | |
− | <td>BBa_K206000+BBa_B0034+Luciferase+BBa_B0015</td>
| + | |
− | <td>Encodes complete luciferase under the control of pBAD promoter</td>
| + | |
− | <td>2454</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891003</a></td>
| + | |
− | <td>N-Luciferase</td>
| + | |
− | <td>Encodes N-terminal of luciferase</td>
| + | |
− | <td>1248</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891004</a></td>
| + | |
− | <td>C-Luciferase</td>
| + | |
− | <td>Encodes C-terminal of luciferase</td>
| + | |
− | <td>459</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891005</a></td>
| + | |
− | <td>α-tubulin+N-Luciferase</td>
| + | |
− | <td>Express α-tubulin- N-Luciferase fusion protein (待改)</td>
| + | |
− | <td>2640</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891006</a></td>
| + | |
− | <td>C-Luciferase+α-tubulin</td>
| + | |
− | <td>Express C-Luciferase-α-tubulin fusion protein(待改)</td>
| + | |
− | <td>1857</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891007</a></td>
| + | |
− | <td>α-tubulin+YNE</td>
| + | |
− | <td>Express α-tubulin-YNE fusion protein</td>
| + | |
− | <td>1866</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891008</a></td>
| + | |
− | <td>α-tubulin+YCE</td>
| + | |
− | <td>Express α-tubulin-YCE fusion protein</td>
| + | |
− | <td>1650</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891009</a></td>
| + | |
− | <td>YNE+α-tubulin</td>
| + | |
− | <td>Express YNE-α-tubulin fusion protein</td>
| + | |
− | <td>1866</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891010</a></td>
| + | |
− | <td>YCE+α-tubulin</td>
| + | |
− | <td>Express YCE-α-tubulin fusion protein</td>
| + | |
− | <td>1650</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891011</a></td>
| + | |
− | <td>β-tubulin+YCE</td>
| + | |
− | <td>Express β-tubulin-YCE fusion protein</td>
| + | |
− | <td>1629</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891012</a></td>
| + | |
− | <td>YCE+β-tubulin</td>
| + | |
− | <td>Express YCE-β-tubulin fusion protein</td>
| + | |
− | <td>1629</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891013</a></td>
| + | |
− | <td>β-tubulin-C- Luciferase</td>
| + | |
− | <td>Express β-tubulin- C-Luciferase fusion protein</td>
| + | |
− | <td>1848</td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891014</a></td>
| + | |
− | <td></td>
| + | |
− | <td></td>
| + | |
− | <td></td>
| + | |
− | </tr>
| + | |
− | <tr>
| + | |
− | <td><a href="#">BBa_1891015</a></td>
| + | |
− | <td></td>
| + | |
− | <td></td>
| + | |
− | <td></td>
| + | |
− | </tr>
| + | |
− | </tbody>
| + | |
− | </table>
| + | |
| | | |
− | <h2>Our Judging Criteria</h2> | + | |
| + | <table class="table table-bordered table-hover"> |
| + | <tr> |
| + | <th>Biobrick</th><th>Name</th><th>Description</th><th>Length</th> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891000">BBa_K1891000</a></td> |
| + | <td>α-tubulin</td> |
| + | <td>Encodes α-tubulin from human breast cancer cell line Mcf-7 </td> |
| + | <td>1356 </td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891001">BBa_K1891001</a></td> |
| + | <td>β-tubulin</td> |
| + | <td>Encodes β-tubulin from human breast cancer cell line Mcf-7 </td> |
| + | <td>1335 </td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891002">BBa_K1891002</a></td> |
| + | <td>BBa_K206000 + BBa_B0034 + Luciferase + BBa_B0015</td> |
| + | <td>Encodes complete luciferase under the control of pBAD promoter.</td> |
| + | <td>2411 </td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891003">BBa_K1891003</a></td> |
| + | <td>N-Luciferase</td> |
| + | <td>Encodes N-terminal of luciferase.</td> |
| + | <td>1248</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891004">BBa_K1891004</a></td> |
| + | <td>C-Luciferase</td> |
| + | <td>Encodes C-terminal of luciferase.</td> |
| + | <td>459</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891005">BBa_K1891005</a></td> |
| + | <td>α-tubulin+N-Luciferase</td> |
| + | <td>Express α-tubulin- N-Luciferase fusion protein</td> |
| + | <td>2646</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891006">BBa_K1891006</a></td> |
| + | <td>C-Luciferase+α-tubulin</td> |
| + | <td>Express C-Luciferase-α-tubulin fusion protein</td> |
| + | <td>1857</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891007">BBa_K1891007</a></td> |
| + | <td>α-tubulin+YNE</td> |
| + | <td>Express α-tubulin-YNE fusion protein.</td> |
| + | <td>1866</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891008">BBa_K1891008</a></td> |
| + | <td>α-tubulin+YCE</td> |
| + | <td>Express α-tubulin-YCE fusion protein</td> |
| + | <td>1650</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891009">BBa_K1891009</a></td> |
| + | <td>YNE+α-tubulin</td> |
| + | <td>Express YNE-α-tubulin fusion protein.</td> |
| + | <td>1866</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891010">BBa_K1891010</a></td> |
| + | <td>β-tubulin+YCE</td> |
| + | <td>Express β-tubulin-YCE fusion protein</td> |
| + | <td>1650</td> |
| + | </tr> |
| + | |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891011">BBa_K1891011</a></td> |
| + | <td>YCE+β-tubulin</td> |
| + | <td>Express YCE-β-tubulin fusion protein</td> |
| + | <td>1629</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891012">BBa_K1891012</a></td> |
| + | <td>YCE+α-tubulin</td> |
| + | <td>Express β-tubulin- C-Luciferase fusion protein</td> |
| + | <td>1929</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891013">BBa_K1891013</a></td> |
| + | <td>C- Luciferase-β-tubulin</td> |
| + | <td>Express C-Luciferase-β-tubulin fusion protein</td> |
| + | <td>1919</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891014">BBa_K1891014</a></td> |
| + | <td>P(3HB) (BBa_K934001) with pBAD promotor (BBa_I0500)</td> |
| + | <td>Express P(3HB) under arabinose inducement</td> |
| + | <td>5426</td> |
| + | </tr> |
| + | |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K1891015">BBa_K1891015</a></td> |
| + | <td>P(3HB) (BBa_K934001) with HSP promotor (BBa_K873002)</td> |
| + | <td>Express P(3HB) under heatshock inducement</td> |
| + | <td id="Judging">4263</td> |
| + | </tr> |
| + | |
| + | |
| + | </table> |
| + | |
| + | <h2 class="text-center">Judging criteria</h2> |
| <div class="panel panel-info bronze"> | | <div class="panel panel-info bronze"> |
| <div class="panel-heading"> | | <div class="panel-heading"> |
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| <ol> | | <ol> |
| <li>We have registered our team, spent a full summer, and plan to enjoy a feast of knowledge and culture at the Giant Jamboree.</li> | | <li>We have registered our team, spent a full summer, and plan to enjoy a feast of knowledge and culture at the Giant Jamboree.</li> |
− | <li>We have met all deliverables on the Requirements page.</li> | + | <li> We have met all deliverables on the Requirements page. </li> |
− | <li>Our story of Taxolight is described using the iGEM wiki. And we have designed, built and characterized several new functional standard biobricks including: Parts</li> | + | <li> Our story of Taxolight is described using the iGEM wiki. And we have designed, built and characterized several new functional standard biobricks including: Parts </li> |
− | <li>1. We made a poster and presentation, which is going to be presented at the Giant Jamboree.</li> | + | <li> We made a poster and presentation, which is going to be presented at the Giant Jamboree. </li> |
| <li>The attributions of our work is clear, and we have distinguished the work done by us and work down by others in the wiki.</li> | | <li>The attributions of our work is clear, and we have distinguished the work done by us and work down by others in the wiki.</li> |
| </ol> | | </ol> |
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| <div class="panel-body"> | | <div class="panel-body"> |
| <ol> | | <ol> |
− | <li>We have designed, built and experimentally validated six new functional biobricks (BBa_K1660000, BBa_K1660001, BBa_K1660002, and BBa_K1660003, BBa_K1660008, BBa_K1660009), (链接)and submitted characterization data to the Registry of Standard Biological Parts. And the experimentally validate that at one new biobrick part of our own design and construction works as expected.</li> | + | <li>We have designed, built and experimentally validated 11 new functional biobricks(<a href="http://parts.igem.org/Part:BBa_K1891000">BBa_K18910000</a>, <a href="http://parts.igem.org/Part:BBa_K1891001">BBa_K1891001</a>, <a href="http://parts.igem.org/Part:BBa_K1891001">BBa_K1891002</a>, <a href="http://parts.igem.org/Part:BBa_K1891001">BBa_K1891005</a>, <a href="http://parts.igem.org/Part:BBa_K1891001">BBa_K1891006</a>, <a href="http://parts.igem.org/Part:BBa_K1891001">BBa_K1891007</a>, <a href="http://parts.igem.org/Part:BBa_K1891001">BBa_K1891008</a>, <a href="http://parts.igem.org/Part:BBa_K1891001">BBa_K1891009</a>, <a href="http://parts.igem.org/Part:BBa_K1891001">BBa_K1891010</a>, <a href="http://parts.igem.org/Part:BBa_K1891001">BBa_K1891011</a>, <a href="http://parts.igem.org/Part:BBa_K1891001">BBa_K1891012</a>) and submitted characterization data to the Registry of Standard Biological Parts. And the experimentally validate that at our new biobrick part of our own design and construction works as expected. |
− | <li>Six new parts that we designed have been submitted to the Registry of Standard Biological Parts.</li> | + | </li> |
− | <li>We offered FAFU-CHINA meticulous help in the modeling. We helped them to establish a model of ordinary differential equations to illustrate the increment of algae population exactly.(链接).</li> | + | <li>Sixteen new parts that we designed have been submitted to the Registry of Standard Biological Parts.</li> |
| + | <li>We offered FAFU-CHINA meticulous help in the modeling. We helped them to establish a model of ordinary differential equations to illustrate the increment of algae population exactly. <a href="https://2016.igem.org/Team:BNU-China/Collaborations">See more in our collaborations.</a></li> |
| <li>Safety: | | <li>Safety: |
| <ol style="margin-left: 45px;list-style: lower-alpha;"> | | <ol style="margin-left: 45px;list-style: lower-alpha;"> |
− | <li><b>Laboratory safety</b>: All of our laboratory members have receieved safety training before. We must fill out the log sheet of the laboratory safety after the inspection tour.</li> | + | <li><b>Laboratory safety</b>: All of our laboratory members have receieved safety training before. We must fill out the log sheet of the laboratory safety after the inspection tour. </li> |
| <li><b>Environmental safety</b>: We have evaluated the project feasibility and its relating security issue. Our kit is consist of proteins and buffer without E.coli . It will reduce the danger that E.coli would do harm to our environment.</li> | | <li><b>Environmental safety</b>: We have evaluated the project feasibility and its relating security issue. Our kit is consist of proteins and buffer without E.coli . It will reduce the danger that E.coli would do harm to our environment.</li> |
| </ol> | | </ol> |
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| <div class="panel-body"> | | <div class="panel-body"> |
| <ol> | | <ol> |
− | <li>We have demonstrated a functional prototype of our project. We made a kit based on the experiment and the data from modeling and wet lab to detect the existence of taxol and other medicines that can inhibit the depolymerization of microtube. And we simulated the way of using our device in the laboratory.</li> | + | <li> We have demonstrated a functional prototype of our project. We made a kit based on the experiment and the data from modeling and wet lab to detect the existence of taxol and other medicines that can inhibit the depolymerization of microtube. And we simulated the way of using our device in the laboratory. </li> |
− | <li>We have characterized a previously existing BioBrick Part, which can produce plastic, (BBa_K934001)(链接). We add two different promoters to make the plastic production under the control of L- Arabinose and temperature. See the improvement (链接) page for more information.</li> | + | <li>We have characterized a previously existing BioBrick Part, which can produce plastic (<a href="http://parts.igem.org/Part:BBa_K934001"> BBa_K934001</a>). We add two different promoters to make the plastic production under the control of L- Arabinose and temperature. See the <a href="https://2016.igem.org/Team:BNU-China/Description">improvement</a> page for more information.</li> |
− | <li>We expand on your Human Practice activity by integrating the investigating issues into the design and execution of your project. During the Investigation of Taxus yunnanensis in Yunnan, we learned that the yew plant bases in Yunnan are mainly allocated at sipsongpanna, Wenshan, Diqing, and so on. The distribution of wild yew trees is dispersive and in small population. They are affected by both natural and human aspects. See the Human Practice(链接) page for more information.</li> | + | <li>We expand on your Human Practice activity by integrating the investigating issues into the design and execution of your project. During the Investigation of Taxus yunnanensis in Yunnan, we learned that the yew plant bases in Yunnan are mainly allocated at sipsongpanna, Wenshan, Diqing, and so on. The distribution of wild yew trees is dispersive and in small population. They are affected by both natural and human aspects. See the <a href="https://2016.igem.org/Team:BNU-China/Integrated_Practices">Human Practice</a> page for more information.</li> |
− | <li>We demonstrated our whole system working under simulated conditions in the lab. We used our fusion protein to detect the exsistence of taxol in our lab.</li> | + | <li> We demonstrated our whole system working under simulated conditions in the lab. We used our fusion protein to detect the exsistence of taxol in our lab.</li> |
| + | <li> <strong>And we won the gold medal finally ! </strong></li> |
| </ol> | | </ol> |
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