Difference between revisions of "Team:Wageningen UR"

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<h2> Introduction </h2>
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<p>Bees are the most important pollinators on earth and may account for a substantial proportion of the food we eat and therefore also for our quality of life [1, 2]. Their importance for the abundance and variety of our diet makes it all the more disturbing that honeybee mortality has been increasing in almost all western countries for at least 30 years [3, 4]. Sometimes even whole bee colonies die in one winter, a phenomenon that has been termed Colony Collapse Disorder (CCD). The most important cause of CCD is the parasitic mite Varroa destructor.  </p>
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<h2> Current treatments </h2>
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<br/>
  
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<p>In the Netherlands, the Varroa mite is now combatted by a year-round treatment with organic compounds that include Thymol, Formic acid and Oxalic acid. However, these compounds have to be applied strictly according to the provided instructions. Thymol, for instance, has a small concentration margin between killing the mites and harming bees. The hobbyist character of beekeeping has to be considered in the context of these treatments. Beekeepers might not have the time and resources to apply the treatments in the intended manner. </p>
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<p>In fulfillment of the requirements for the Gold Medal, the 2016 Wageningen iGEM team accomplished the following:</p>
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<br>
  
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<h2>Synthetic Biological Treatment </h2>
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<h2>Bronze Medal Requirements：</h2>
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<p>We aim to create a new and better solution for the Varroa problem using synthetic biology. Our project would yield a product harmless to both humans and bees because we intend to use a Bacillus thuringiensis protein toxin to target Varroa. B. thuringiensis is known for the production of very specific insecticidal protein endotoxins. Multiple Bacillus strains have now been identified that target the Varroa mite. We will employ the toxins from these strains to relieve bee colonies from this most harmful parasite. In addition to the B. thuringiensis toxin, our synthetic biological system will contain systems to provide efficient production and release of the toxin and to provide users with a high level of control. </p>
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<img src="https://static.igem.org/mediawiki/2016/6/6a/T--Wageningen_UR--bronze.jpg" align="right" width="100">
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<ol>
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<li style="text-align:left"> Registered for iGEM and will present the project at the 2016 Giant Jamboree.
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</li>
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<li style="text-align:left">
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Created and submitted to the Registry 31 new <a href="https://2016.igem.org/Team:Wageningen_UR/Parts">BioBricks</a>
  
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</li>
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<li style="text-align:left">Created a documented <a href="https://2016.igem.org/Team:Wageningen_UR/Attributions">attributions page</a> in our wiki.
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</li>
  
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<li style="text-align:left">Completed the Safety and Judging forms and met all other required deliverables.</li>
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</ol>
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<br>
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<h2>Silver Medal Requirements：</h2>
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<img src="https://static.igem.org/mediawiki/2016/a/a5/T--Wageningen_UR--silver.jpg" align="right" width="100">
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<ol>
  
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<h2> References </h2>
 
  
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<li>
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<li>Validated the functionality of several of the submitted BioBricks.</li>
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<ol> 1. McGregor, Samuel Emmett. Insect pollination of cultivated crop plants. Vol. 496. Agricultural Research Service, US Department of Agriculture, 1976. </ol>
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<ol>
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<ul>
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2. Delaplane, Keith S. "On Einstein, Bees, and Survival of the Human Race." Entomology: UGA Honey Bee Program (2009). </ol>
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<li><a href="http://parts.igem.org/Part:BBa_K1913005">BBa_K1913005</a> encodes a quorum sensing system with GFP as a system output. We characterized this BioBrick, showing its functionality. </li>
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<li> <a href="http://parts.igem.org/Part:BBa_K1913008">BBa_K1913008,</a> <a href="http://parts.igem.org/Part:BBa_K1913009">BBa_K1913009</a> are a Vitamin B12 and a guanine riboswitch, respectively. Both of them were functionally validated <i>in vivo</i> using RFP as reporter.</li>
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<li><a href="http://parts.igem.org/Part:BBa_K1913015">BBa_K1913015</a> encodes a Cry3Aa1-like protein that has a high level of toxicity towards most kinds of insects. We experimentally validated its toxicity <i>in vitro</i> by using brush border membrane vesicles (BBMV).</li>
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 +
 
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<li>  <a href="http://parts.igem.org/Part:BBa_K1913025">BBa_K1913025</a> and <a href="http://parts.igem.org/Part:BBa_K1913026">BBa_K1913026</a> are FixK2/plac hybrid promoters, which were functionally validated in combination with a light sensor generator(<a href="http://parts.igem.org/Part:BBa_K1913034">BBa_K1913034</a>).</li></ul>
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 +
<li>Considered safety in our project by implementing a optogenetic kill switch system and a synthetic amino acid system to prevent horizontal gene transfer. By implementing these systems, we will reduce the environmental impact of BeeT.</li>
 +
 
 +
 
 +
<li>Collaborated with the TU Delft and Groningen iGEM teams.</li>
 +
 
 +
<li>As human practice activities, we discussed our project application scenario with Synenergene and we wrote a future scenario involving BeeT application in collaboration with them. We also organized the national Dutch iGEM conference with other iGEM teams to share our experiences. </li> </ol>
 +
<br>
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 +
<h2>Gold Medal Requirements:</h2>
 +
<img src="https://static.igem.org/mediawiki/2016/6/6a/T--Wageningen_UR--gold.jpg" align="right" width="100">
 
<ol>
 
<ol>
−
3. Potts, Simon G., et al. "Declines of managed honey bees and beekeepers in Europe." Journal of Apicultural Research 49.1 (2010): 15-22. </ol>
 
−
<ol>
 
−
4. Van der Zee, Romée, et al. "Managed honey bee colony losses in Canada, China, Europe, Israel and Turkey, for the winters of 2008–9 and 2009–10." Journal of Apicultural Research 51.1 (2012): 100-114. </ol>
 
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</li>
 
  
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</div>  
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<li>Improved and verify several previous parts:</li>
  
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<ul>
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<li>
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We improved the characterization of <a href="http://parts.igem.org/Part:BBa_K546000">BBa_K546000</a>, a quorum sensing composite part that we tested together with <a href="http://parts.igem.org/Part:BBa_K1913005">BBa_K1913005</a>, resulting in these <a href="http://parts.igem.org/Part:BBa_K546000:Experience">fluorescence graphs</a>.  </li>
 +
 +
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<li>Based on the original FixK2 promoter (<a href="http://parts.igem.org/Part:BBa_K592006">BBa_K592006</a> made by Uppsala-Sweden_2011), we designed and provided new <a href="http://parts.igem.org/Part:BBa_K1913025">FixK2 promoters</a> by adding a core control element and operons to the canonical FixJ binding sites, making them controllable by different input signals.</li>
 +
 +
</ul>
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 +
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<li> Integrated Human Practices:</li>
 +
 +
 +
<p>We consulted several stakeholders and specialists such as beekeepers from the Dutch Beekeeper association and researchers from Wageningen University to get suggestions in order to make our BeeT product more feasible and acceptable. According to their suggestions, our product should fit with the hobbyist character of beekeeping and never affect honey production. We amended our project design following their suggestions. In addition, we collaborated with Design Academy Eindhoven to design a tea bag-like product that beekeepers can easily apply. </p>
 +
 +
<li>Demonstrate: </li>
 +
<p>We <a href="https://2016.igem.org/Team:Wageningen_UR/Demonstrate">demonstrated</a> some of BeeT's functionalities in conditions which simulate its real-life application. </p>
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<h2>Sponsors</h2>
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<div class="sponsor_box">
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<a class="sponsor_rectangle" href="http://www.wageningenur.nl/en/Benefactors/Service/About-Wageningen-University-Fund.htm" target="blank"><img src="https://static.igem.org/mediawiki/2014/6/6f/Wageningen_UR_WUF_EN.png" style="width:150px"></a>
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<a class="sponsor_rectangle" href="http://www.bijenhouders.nl/" target="blank"><img src="https://static.igem.org/mediawiki/2016/b/be/T--Wageningen_UR--NBV.png"></a>
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<a class="sponsor_rectangle" href="http://www.synenergene.eu/" target="blank"><img src="https://static.igem.org/mediawiki/2014/4/48/Wageningen_UR_synenergene.png"></a>
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<a class="sponsor_rectangle" href="http://www.snapgene.com/" target="blank"><img src="https://static.igem.org/mediawiki/2014/f/f0/Wageningen_UR_snapgene.png"></a>
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<a class="sponsor_rectangle" href="https://www.eppendorf.com/NL-en/" target="blank"><img src="https://static.igem.org/mediawiki/2016/8/86/T--Wageningen_UR--Eppendorf.jpg"></a>
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<a class="sponsor_rectangle"
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href="http://www.ziemer-falke.de/" target="blank"><img src="https://static.igem.org/mediawiki/2016/4/4a/T--Wageningen_UR--ziemer-falke-logo-300x74.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.neb.com/" target="blank"><img src="https://static.igem.org/mediawiki/2014/d/da/Wageningen_UR_neb.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.dsm.com/corporate/home.html" target="blank"><img src="https://static.igem.org/mediawiki/2014/9/9f/Wageningen_UR_dsm.png"></a>
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<a class="sponsor_rectangle" href="http://www.rivm.nl/" target="blank"><img src="https://static.igem.org/mediawiki/2016/4/4a/T--Wageningen_UR--RO_VWS_RIVM_Logo_1_2965C_pos_nl_0.jpg"></a>
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<a class="sponsor_rectangle" href="https://www.gatc-biotech.com/en/index.html" target="blank"><img src="https://static.igem.org/mediawiki/2016/2/28/T--Wageningen_UR--logoGATC-300x150.gif"></a>
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<a class="sponsor_rectangle" href="http://www.inbuzzextra.nl/home" target="blank"><img src="https://static.igem.org/mediawiki/2016/9/96/IIT--Wageningen_UR--inbuzz.jpg"></a>
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<a class="sponsor_rectangle" href="https://crowdfunding.wur.nl/" target="blank"><img src="https://static.igem.org/mediawiki/2016/d/d2/T--Wageningen_UR--crowdfunding.jpg"></a>
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<div class="clear">
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<a class="sponsor_square" href="http://www.vlaggraduateschool.nl/" target="blank"><img src="https://static.igem.org/mediawiki/2014/8/85/Wageningen_UR_vlag.jpg"></a>
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<a class="sponsor_square" href="https://www.bioke.com/" target="blank"><img src="https://static.igem.org/mediawiki/2013/6/65/UGent_2013_BiokeTrans.png"></a>
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<a class="sponsor_square" href="http://dierenspeciaalzaakxxl.com/content/105-venray-dierenarts" target="blank"><img src="https://static.igem.org/mediawiki/2016/3/3c/T--Wageningen_UR--GD.JPG"></a>
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<div class="clear"></div>
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<a class="sponsor_square" href="http://eu.idtdna.com/site" target="blank"><img src="https://static.igem.org/mediawiki/2016/2/2b/T--Wageningen_UR--IDT-Logo-300x124.jpg"></a>
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<a class="sponsor_wur" href="http://www.wageningenur.nl/en.htm" target="blank"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Wageningen_UR--sponsors_list_newone.jpg" style="width:100%; height:100%; "></a> </div>
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</div>
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Latest revision as of 13:05, 22 November 2016

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Wageningen UR iGEM 2016

 

 

 


BeeT Outreach MetabolicModel BeeHave Riboswitch Quorum toxin biocontainment comic

In fulfillment of the requirements for the Gold Medal, the 2016 Wageningen iGEM team accomplished the following:


Bronze Medal Requirements：

  1. Registered for iGEM and will present the project at the 2016 Giant Jamboree.
  2. Created and submitted to the Registry 31 new BioBricks
  3. Created a documented attributions page in our wiki.
  4. Completed the Safety and Judging forms and met all other required deliverables.

Silver Medal Requirements：

  1. Validated the functionality of several of the submitted BioBricks.
    • BBa_K1913005 encodes a quorum sensing system with GFP as a system output. We characterized this BioBrick, showing its functionality.
    • BBa_K1913008, BBa_K1913009 are a Vitamin B12 and a guanine riboswitch, respectively. Both of them were functionally validated in vivo using RFP as reporter.
    • BBa_K1913015 encodes a Cry3Aa1-like protein that has a high level of toxicity towards most kinds of insects. We experimentally validated its toxicity in vitro by using brush border membrane vesicles (BBMV).
    • BBa_K1913025 and BBa_K1913026 are FixK2/plac hybrid promoters, which were functionally validated in combination with a light sensor generator(BBa_K1913034).
  2. Considered safety in our project by implementing a optogenetic kill switch system and a synthetic amino acid system to prevent horizontal gene transfer. By implementing these systems, we will reduce the environmental impact of BeeT.
  3. Collaborated with the TU Delft and Groningen iGEM teams.
  4. As human practice activities, we discussed our project application scenario with Synenergene and we wrote a future scenario involving BeeT application in collaboration with them. We also organized the national Dutch iGEM conference with other iGEM teams to share our experiences.

Gold Medal Requirements:

  1. Improved and verify several previous parts:
    • We improved the characterization of BBa_K546000, a quorum sensing composite part that we tested together with BBa_K1913005, resulting in these fluorescence graphs.
    • Based on the original FixK2 promoter (BBa_K592006 made by Uppsala-Sweden_2011), we designed and provided new FixK2 promoters by adding a core control element and operons to the canonical FixJ binding sites, making them controllable by different input signals.
  2. Integrated Human Practices:

    3. We consulted several stakeholders and specialists such as beekeepers from the Dutch Beekeeper association and researchers from Wageningen University to get suggestions in order to make our BeeT product more feasible and acceptable. According to their suggestions, our product should fit with the hobbyist character of beekeeping and never affect honey production. We amended our project design following their suggestions. In addition, we collaborated with Design Academy Eindhoven to design a tea bag-like product that beekeepers can easily apply.

  3. Demonstrate:

    4. We demonstrated some of BeeT's functionalities in conditions which simulate its real-life application.


    Sponsors