Difference between revisions of "Team:Wageningen UR"

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<comic>
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<img src="https://static.igem.org/mediawiki/2016/7/70/T--Wageningen_UR--achievements.jpg">
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</comic>
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<br>
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<comic>
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<img src="https://static.igem.org/mediawiki/2016/3/30/T--Wageningen_UR--introcomic.jpg" width="600">
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</comic>
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<map name="comicmap">
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<area alt="BeeT" href="https://2016.igem.org/Team:Wageningen_UR/Description" shape="poly" coords="34,45,764,48,763,244,278,250,33,500" />
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<area alt="Outreach" title="Outreach" href="https://2016.igem.org/Team:Wageningen_UR/HP/Gold" shape="poly" coords="309,273,768,275,767,546,34,545" />
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<area alt="MetabolicModel" title="MetabolicModel" href="https://2016.igem.org/Team:Wageningen_UR/Model#metabolic" shape="rect" coords="32,570,487,846" />
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<area alt="BeeHave" title="BeeHave" href="https://2016.igem.org/Team:Wageningen_UR/Model#beehave" shape="rect" coords="512,569,769,847" />
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<area alt="Riboswitch" title="Riboswitch" href="https://2016.igem.org/Team:Wageningen_UR/Description/Regulation#DetectingMites" shape="poly" coords="33,872,394,874,218,1095,33,1098" />
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<area alt="Quorum" title="Quorum" href="https://2016.igem.org/Team:Wageningen_UR/Description/Regulation#QuorumSensing" shape="poly" coords="436,876,768,875,768,1096,255,1096" />
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<area alt="toxin" title="toxin" href="https://2016.igem.org/Team:Wageningen_UR/Description/Specificity" shape="poly" coords="34,1118,764,1120,489,1392,35,1389" />
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<area alt="biocontainment" title="biocontainment" href="https://2016.igem.org/Team:Wageningen_UR/Description/Biocontainment" shape="poly" coords="769,1160,766,1392,538,1390" />
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</map>
  
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<div class="column full_size" >
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<comic>
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<img src="http://placehold.it/800x300/d3d3d3/f2f2f2">
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<img src="https://static.igem.org/mediawiki/2016/2/2c/T--Wageningen_UR--comic.png" usemap="#comicmap" alt="comic"/>
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</div>
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</comic>
  
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<div class="column full_size" >
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<br/>
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<h2> Welcome to iGEM 2016! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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</div>  
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<p>In fulfillment of the requirements for the Gold Medal, the 2016 Wageningen iGEM team accomplished the following:</p>
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<br>
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<h2>Bronze Medal Requirements：</h2>
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<img src="https://static.igem.org/mediawiki/2016/6/6a/T--Wageningen_UR--bronze.jpg" align="right" width="100">
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<ol>
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<li style="text-align:left"> Registered for iGEM and will present the project at the 2016 Giant Jamboree.
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</li>
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<li style="text-align:left">
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Created and submitted to the Registry 31 new <a href="https://2016.igem.org/Team:Wageningen_UR/Parts">BioBricks</a>
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</li>
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<li style="text-align:left">Created a documented <a href="https://2016.igem.org/Team:Wageningen_UR/Attributions">attributions page</a> in our wiki.
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</li>
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<li style="text-align:left">Completed the Safety and Judging forms and met all other required deliverables.</li>
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</ol>
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<br>
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<h2>Silver Medal Requirements：</h2>
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<img src="https://static.igem.org/mediawiki/2016/a/a5/T--Wageningen_UR--silver.jpg" align="right" width="100">
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<ol>
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<li>Validated the functionality of several of the submitted BioBricks.</li>
  
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<div class="column half_size" >
 
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<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
 
<ul>
 
<ul>
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
 
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
 
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
 
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</ul>
 
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</div>
 
  
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<div class="column half_size" >
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<li><a href="http://parts.igem.org/Part:BBa_K1913005">BBa_K1913005</a> encodes a quorum sensing system with GFP as a system output. We characterized this BioBrick, showing its functionality. </li>
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<div class="highlight">
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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</div>
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</div>
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<div class="column full_size" >
 
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<h5> Wiki template information </h5>
 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
  
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</div>  
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<li> <a href="http://parts.igem.org/Part:BBa_K1913008">BBa_K1913008,</a> <a href="http://parts.igem.org/Part:BBa_K1913009">BBa_K1913009</a> are a Vitamin B12 and a guanine riboswitch, respectively. Both of them were functionally validated <i>in vivo</i> using RFP as reporter.</li>
  
  
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<li><a href="http://parts.igem.org/Part:BBa_K1913015">BBa_K1913015</a> encodes a Cry3Aa1-like protein that has a high level of toxicity towards most kinds of insects. We experimentally validated its toxicity <i>in vitro</i> by using brush border membrane vesicles (BBMV).</li>
  
  
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<div class="column half_size" >
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<li>  <a href="http://parts.igem.org/Part:BBa_K1913025">BBa_K1913025</a> and <a href="http://parts.igem.org/Part:BBa_K1913026">BBa_K1913026</a> are FixK2/plac hybrid promoters, which were functionally validated in combination with a light sensor generator(<a href="http://parts.igem.org/Part:BBa_K1913034">BBa_K1913034</a>).</li></ul>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
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</div>
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<li>Considered safety in our project by implementing a optogenetic kill switch system and a synthetic amino acid system to prevent horizontal gene transfer. By implementing these systems, we will reduce the environmental impact of BeeT.</li>
  
  
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<div class="column half_size" >
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<li>Collaborated with the TU Delft and Groningen iGEM teams.</li>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>
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</div>
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 +
<li>As human practice activities, we discussed our project application scenario with Synenergene and we wrote a future scenario involving BeeT application in collaboration with them. We also organized the national Dutch iGEM conference with other iGEM teams to share our experiences. </li> </ol>
 +
<br>
 +
 +
<h2>Gold Medal Requirements:</h2>
 +
<img src="https://static.igem.org/mediawiki/2016/6/6a/T--Wageningen_UR--gold.jpg" align="right" width="100">
 +
<ol>
 +
 +
<li>Improved and verify several previous parts:</li>
  
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<div class="column half_size" >
 
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<h5>Inspiration</h5>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
 
<ul>
 
<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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We improved the characterization of <a href="http://parts.igem.org/Part:BBa_K546000">BBa_K546000</a>, a quorum sensing composite part that we tested together with <a href="http://parts.igem.org/Part:BBa_K1913005">BBa_K1913005</a>, resulting in these <a href="http://parts.igem.org/Part:BBa_K546000:Experience">fluorescence graphs</a>.  </li>
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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 +
<li>Based on the original FixK2 promoter (<a href="http://parts.igem.org/Part:BBa_K592006">BBa_K592006</a> made by Uppsala-Sweden_2011), we designed and provided new <a href="http://parts.igem.org/Part:BBa_K1913025">FixK2 promoters</a> by adding a core control element and operons to the canonical FixJ binding sites, making them controllable by different input signals.</li>  
 +
 
 
</ul>
 
</ul>
−
</div>
 
  
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<div class="column half_size" >
 
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<h5> Uploading pictures and files </h5>
 
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
 +
<li> Integrated Human Practices:</li>
 +
 +
 +
<p>We consulted several stakeholders and specialists such as beekeepers from the Dutch Beekeeper association and researchers from Wageningen University to get suggestions in order to make our BeeT product more feasible and acceptable. According to their suggestions, our product should fit with the hobbyist character of beekeeping and never affect honey production. We amended our project design following their suggestions. In addition, we collaborated with Design Academy Eindhoven to design a tea bag-like product that beekeepers can easily apply. </p>
  
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<div class="button_click"  onClick=" parent.location= 'https://2016.igem.org/Special:Upload '">
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<li>Demonstrate: </li>
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UPLOAD FILES
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<p>We <a href="https://2016.igem.org/Team:Wageningen_UR/Demonstrate">demonstrated</a> some of BeeT's functionalities in conditions which simulate its real-life application. </p>
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</div>
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</div>
 
  
  
  
  
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<br/>
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<h2>Sponsors</h2>
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<div class="sponsor_box">
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<a class="sponsor_rectangle" href="http://www.wageningenur.nl/en/Benefactors/Service/About-Wageningen-University-Fund.htm" target="blank"><img src="https://static.igem.org/mediawiki/2014/6/6f/Wageningen_UR_WUF_EN.png" style="width:150px"></a>
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<a class="sponsor_rectangle" href="http://www.bijenhouders.nl/" target="blank"><img src="https://static.igem.org/mediawiki/2016/b/be/T--Wageningen_UR--NBV.png"></a>
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<a class="sponsor_rectangle" href="http://www.synenergene.eu/" target="blank"><img src="https://static.igem.org/mediawiki/2014/4/48/Wageningen_UR_synenergene.png"></a>
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<a class="sponsor_rectangle" href="http://www.snapgene.com/" target="blank"><img src="https://static.igem.org/mediawiki/2014/f/f0/Wageningen_UR_snapgene.png"></a>
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<a class="sponsor_rectangle" href="https://www.eppendorf.com/NL-en/" target="blank"><img src="https://static.igem.org/mediawiki/2016/8/86/T--Wageningen_UR--Eppendorf.jpg"></a>
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<a class="sponsor_rectangle"
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href="http://www.ziemer-falke.de/" target="blank"><img src="https://static.igem.org/mediawiki/2016/4/4a/T--Wageningen_UR--ziemer-falke-logo-300x74.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.neb.com/" target="blank"><img src="https://static.igem.org/mediawiki/2014/d/da/Wageningen_UR_neb.jpg"></a>
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<a class="sponsor_rectangle" href="http://www.dsm.com/corporate/home.html" target="blank"><img src="https://static.igem.org/mediawiki/2014/9/9f/Wageningen_UR_dsm.png"></a>
 +
<a class="sponsor_rectangle" href="http://www.rivm.nl/" target="blank"><img src="https://static.igem.org/mediawiki/2016/4/4a/T--Wageningen_UR--RO_VWS_RIVM_Logo_1_2965C_pos_nl_0.jpg"></a>
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<a class="sponsor_rectangle" href="https://www.gatc-biotech.com/en/index.html" target="blank"><img src="https://static.igem.org/mediawiki/2016/2/28/T--Wageningen_UR--logoGATC-300x150.gif"></a>
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<a class="sponsor_rectangle" href="http://www.inbuzzextra.nl/home" target="blank"><img src="https://static.igem.org/mediawiki/2016/9/96/IIT--Wageningen_UR--inbuzz.jpg"></a>
 +
<a class="sponsor_rectangle" href="https://crowdfunding.wur.nl/" target="blank"><img src="https://static.igem.org/mediawiki/2016/d/d2/T--Wageningen_UR--crowdfunding.jpg"></a>
 +
<div class="clear">
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<a class="sponsor_square" href="http://www.vlaggraduateschool.nl/" target="blank"><img src="https://static.igem.org/mediawiki/2014/8/85/Wageningen_UR_vlag.jpg"></a>
  
 +
<a class="sponsor_square" href="https://www.bioke.com/" target="blank"><img src="https://static.igem.org/mediawiki/2013/6/65/UGent_2013_BiokeTrans.png"></a>
 +
<a class="sponsor_square" href="http://dierenspeciaalzaakxxl.com/content/105-venray-dierenarts" target="blank"><img src="https://static.igem.org/mediawiki/2016/3/3c/T--Wageningen_UR--GD.JPG"></a>
 +
<div class="clear"></div>
  
 +
<a class="sponsor_square" href="http://eu.idtdna.com/site" target="blank"><img src="https://static.igem.org/mediawiki/2016/2/2b/T--Wageningen_UR--IDT-Logo-300x124.jpg"></a>
  
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<a class="sponsor_wur" href="http://www.wageningenur.nl/en.htm" target="blank"><img src="https://static.igem.org/mediawiki/2016/c/c6/T--Wageningen_UR--sponsors_list_newone.jpg" style="width:100%; height:100%; "></a> </div>
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</div>
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</html>
 
</html>
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{{Wageningen_UR/footer}}

Latest revision as of 13:05, 22 November 2016

MENU ▤
Wageningen UR iGEM 2016

 

 

 


BeeT Outreach MetabolicModel BeeHave Riboswitch Quorum toxin biocontainment comic

In fulfillment of the requirements for the Gold Medal, the 2016 Wageningen iGEM team accomplished the following:


Bronze Medal Requirements：

  1. Registered for iGEM and will present the project at the 2016 Giant Jamboree.
  2. Created and submitted to the Registry 31 new BioBricks
  3. Created a documented attributions page in our wiki.
  4. Completed the Safety and Judging forms and met all other required deliverables.

Silver Medal Requirements：

  1. Validated the functionality of several of the submitted BioBricks.
    • BBa_K1913005 encodes a quorum sensing system with GFP as a system output. We characterized this BioBrick, showing its functionality.
    • BBa_K1913008, BBa_K1913009 are a Vitamin B12 and a guanine riboswitch, respectively. Both of them were functionally validated in vivo using RFP as reporter.
    • BBa_K1913015 encodes a Cry3Aa1-like protein that has a high level of toxicity towards most kinds of insects. We experimentally validated its toxicity in vitro by using brush border membrane vesicles (BBMV).
    • BBa_K1913025 and BBa_K1913026 are FixK2/plac hybrid promoters, which were functionally validated in combination with a light sensor generator(BBa_K1913034).
  2. Considered safety in our project by implementing a optogenetic kill switch system and a synthetic amino acid system to prevent horizontal gene transfer. By implementing these systems, we will reduce the environmental impact of BeeT.
  3. Collaborated with the TU Delft and Groningen iGEM teams.
  4. As human practice activities, we discussed our project application scenario with Synenergene and we wrote a future scenario involving BeeT application in collaboration with them. We also organized the national Dutch iGEM conference with other iGEM teams to share our experiences.

Gold Medal Requirements:

  1. Improved and verify several previous parts:
    • We improved the characterization of BBa_K546000, a quorum sensing composite part that we tested together with BBa_K1913005, resulting in these fluorescence graphs.
    • Based on the original FixK2 promoter (BBa_K592006 made by Uppsala-Sweden_2011), we designed and provided new FixK2 promoters by adding a core control element and operons to the canonical FixJ binding sites, making them controllable by different input signals.
  2. Integrated Human Practices:

    3. We consulted several stakeholders and specialists such as beekeepers from the Dutch Beekeeper association and researchers from Wageningen University to get suggestions in order to make our BeeT product more feasible and acceptable. According to their suggestions, our product should fit with the hobbyist character of beekeeping and never affect honey production. We amended our project design following their suggestions. In addition, we collaborated with Design Academy Eindhoven to design a tea bag-like product that beekeepers can easily apply.

  3. Demonstrate:

    4. We demonstrated some of BeeT's functionalities in conditions which simulate its real-life application.


    Sponsors