Difference between revisions of "Team:UBonn HBRS"

 
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<!--An impressive 214,364,825 tons of paper were recycled in 2013 worldwide. The most relevant step for the quality of recycled paper is the removal of ink (deinking).  Although chlorine has been widely banished from deinking, the process remains environmentally and economically challenging. In particular, the production of chemicals employed for deinking is energy intensive and the by-products are environmentally harmful. We want to tackle this issue by using enzymes instead of chemicals.
 
  
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Enzymes have already been shown to efficiently deink paper. However, application of enzymes in industry requires a highly optimised procedure. For this, it would be ideal to develop a cheap and easy to use system based on continuous secretion of various enzymes, which could be then used to test their deinking efficiency.  
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<img class="img-20 img-xs50" src="//2016.igem.org/wiki/images/8/8a/T--UBonn_HBRS--igem_silver_award.png" alt="iGEM Silver Award" />
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<h2 class="dark-title">Giant Jamboree Results</h2>
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<li>Won Silver Award</li>
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<li>Nominated for Best Poster</li>
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Our approach relies on gene expression in Bacillus subtilis, as this species naturally secretes large amounts of proteins into its surrounding. Using B. Subtilis allows us to purify or even use our enzymes directly from the supernatant. We have developed a reliable expression plasmid for B. subtilis for the iGEM parts registry, which allows replication in Escherichia coli, as well as in B. subtilis. Finally, bacterial supernatants containing the expressed enzymes will be tested in the deinking process.
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We aim revolutionize paper recycling by integrating all of the above aspects into one procedure that is simple enough to be applied not only in centralized recycling facilities, but also for implementation on a smaller scale. This would dramatically reduce the distance paper waste needs to be transported, thereby reducing the carbon footprint of the entire process.
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Our system is the prerequisite to improve enzymatic deinking to a level where it is not only industrial feasible, but outperforms chemical deinking on this scale.-->
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<h2 class="cyan-title">Our project in a nutshell</h2>
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<h2> Welcome to iGEM 2016! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<p>Recycling of printed paper has made an indisputable contribution to conserving our environment. <br />
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However, current recycling methods consume vast amounts of energy and release toxic byproducts.</p>
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<img class="img-responsive center-block" src="//2016.igem.org/wiki/images/5/53/T--UBonn_HBRS--dead_fish.svg" alt="Dead Fish">
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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<h5> Styling your wiki </h5>
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<p>Prior research has identified a number of enzyme classes that can efficiently replace the chemicals currently used in paper recycling. Despite their ability to facilitate the separation of ink particles from paper fibers, a process called deinking, enzymes have not been widely used industrially due to insufficient knowledge about efficiency and costly production and purification methods.</p>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<img class="img-responsive center-block" src="//2016.igem.org/wiki/images/0/0e/T--UBonn_HBRS--fibers.svg" alt="Fibers">
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<p>In our project, we tackled these issues and took significant steps towards catalyzing research in the area and applying enzymatic paper recycling on an industrial scale. We developed a high throughput system which not only allows us to quantify deinking efficiency, but also to cheaply mass produce enzymes using a <i>Bacillus subtilis</i> secretion system.</p>
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<img class="img-responsive center-block" src="//2016.igem.org/wiki/images/e/e7/T--UBonn_HBRS--petri_dish.svg" alt="Petri Dish">
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<h2 class="magenta-title">Have you already seen our Sponsors?</h2>
  
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We are very thankful to our Sponsors for making our project possible! <br />
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Please check them out on our dedicated <br />
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<a class="btn btn-default" href="//2016.igem.org/Team:UBonn_HBRS/Attributions/Sponsors">Sponsors Page</a>
  
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<h5> Editing your wiki </h5>
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<img src="//2016.igem.org/wiki/images/5/5f/T--UBonn_HBRS--paper-plane.jpg" alt="" class="img-responsive" style="margin-bottom: -15px" />
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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Latest revision as of 23:12, 30 November 2016

 

Enzymatic Whitewashing - the ecological approach to paper recycling


iGEM Silver Award

Giant Jamboree Results

  • Won Silver Award
  • Nominated for Best Poster


Our project in a nutshell

Recycling of printed paper has made an indisputable contribution to conserving our environment.
However, current recycling methods consume vast amounts of energy and release toxic byproducts.

Dead Fish

Prior research has identified a number of enzyme classes that can efficiently replace the chemicals currently used in paper recycling. Despite their ability to facilitate the separation of ink particles from paper fibers, a process called deinking, enzymes have not been widely used industrially due to insufficient knowledge about efficiency and costly production and purification methods.

Fibers

In our project, we tackled these issues and took significant steps towards catalyzing research in the area and applying enzymatic paper recycling on an industrial scale. We developed a high throughput system which not only allows us to quantify deinking efficiency, but also to cheaply mass produce enzymes using a Bacillus subtilis secretion system.

Petri Dish

Have you already seen our Sponsors?

We are very thankful to our Sponsors for making our project possible!
Please check them out on our dedicated

Sponsors Page

© iGEM Team Bonn 2016 Top ↑