Difference between revisions of "Team:UBonn HBRS/Attributions"

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{{UBonn_HBRS}}
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{{UBonn_HBRS/header|title=Attributions|Attributions=active}}
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We are very thankful for the lots of help we received! Please also check out our great Sponsors, whom we dedicated a special page: <br /><html><a class="btn btn-default" href="//2016.igem.org/Team:UBonn_HBRS/Attributions/Sponsors">Our Sponsors</a></html>
  
  
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<div class="column full_size judges-will-not-evaluate">
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== General support ==
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<h3>★  ALERT! </h3>
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We thank the '''<html><a href="https://www.uni-bonn.de/" target="_blank">Rheinische Friedrich-Wilhelms-University of Bonn</a></html>''', the '''<html><a href="http://limes-institut-bonn.de/" target="_blank">Limes Institute Bonn</a></html>''' and the '''<html><a href="https://www.h-brs.de/" target="_blank">University of Applied Sciences Bonn-Rhein-Sieg<html></a></html>''' for providing the laboratory space, material and funding.
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">Attributions bronze criterion</a>. </p>
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== Scientific supervision ==
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We are very grateful to '''Prof. Dr. Günther Mayer''' and '''Prof. Dr. Michael Famulok''' of University Bonn for supervising this project.
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We would also like to thank '''Prof. Dr. Klaus Lehmann''' of HBRS for his resourceful and unlimited support.
  
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
 
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</div>
 
  
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== Lab support ==
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Our dearest thanks go to our instructor '''Ignacio Geraci''' and all the other PhDs, PhD students and technicians of '''<html><a href="http://www.limes-institut-bonn.de/forschung/arbeitsgruppen/unit-4/abteilung-mayer/mitarbeiter/labormitarbeiter/" target="_blank">Professor Mayer’s</a></html>''' and '''<html><a href="http://www.limes-institut-bonn.de/forschung/arbeitsgruppen/unit-4/abteilung-famulok/mitarbeiter/" target="_blank">Prof. Famulok’s</a></html>''' laboratory, especially '''Thinhinane Chaou, Laia Civit, Laura Eiden, Jeffrey Hannam, Mark Kerzhner, Daniel Matzner, Franziska Pfeiffer,  Anna Pyka, Christian Renzl, Malte Rosenthal, Anna Schüller, Julia Siegl, Justina Stark, Jörg Ewers, Sebastian Pilsl, Silvana Haßel''' and '''Olga Wolter''' for their help and patience. We would also like to give special thanks to '''Stefan Künne''' for all his support and supervision throughout the project.
  
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<html><img src="//2016.igem.org/wiki/images/5/55/T--UBonn_HBRS--heart.svg" alt="Heart" class="col-xs-4 col-md-2" style="float:none"/></html>
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<br />
  
  
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== ''B. subtilis'' support ==
  
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For their support with electroporation and the use of their equipment we would like to thank all members of the AG Dickschadt from the chemical institute of the University of Bonn. Furthermore, we would like to thank Prof. Satoshi Matsuoka for the gracious donation of ''B. subtilis'' expression plasmids containing three enzymes of interest and Prof. Thomas Schweder from the Institute of Pharmaceutical Biotechnology at the University of Greifswald for the use of two ''Bacillus subtilis'' strains LS8P-D and ATCC 6051.
  
  
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== Equipment Support ==
  
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<div class="column full_size">
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For the use of titration equipment we would like to thank Dr. Ralf Weisbarth from the Chemical Institute at the University of Bonn and for the use of a lipase kit we would like to thank Prof. Christoph Thiele from the LIMES institute at the University of Bonn.
  
  
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<p> Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster. </p>
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== Project advisor support ==
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* '''Benedikt HÖLBLING''' <br />took care of the team’s finances, initiated and organized the team's science slams.
  
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</div>
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* '''BÙI Hoàng Duy''' <br />gave pieces of advice to the project.
  
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* '''Niklas Arndt SCHMACKE''' <br />Was involved in the development of the project idea, introduced new team members to the theoretical and practical aspects of the project and iGEM and served as a remote advisor from Switzerland for a large part of the project.
  
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<div class="clear"></div>
 
  
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== Team Attributions ==
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Our Team Members worked in the following groups, learn more about our team on our <br />
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<html><a class="btn btn-default" href="//2016.igem.org/Team:UBonn_HBRS/Team">Team Page</a></html>
  
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<div class="column half_size">
 
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<h5> Why is this page needed? </h5>
 
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
 
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<p>
 
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
 
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</div>
 
  
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=== ''B. subtilis'' ===
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* '''Ariana Natalie KLAPPERT''' <br />optimzied PAGE gel analysis and protein expression.
  
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<div class="column half_size">
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* '''Jonas GOCKEL''' <br />prepared competent bacteria, tested transformation protocols and established the ''Bacillus Subtilis'' collaboration with Freiburg.
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<h5> What should this page have?</h5>
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<ul>
 
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<li>General Support</li>
 
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<li>Project support and advice</li>
 
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<li>Fundraising help and advice</li>
 
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<li>Lab support</li>
 
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<li>Difficult technique support</li>
 
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<li>Project advisor support</li>
 
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<li>Wiki support</li>
 
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<li>Presentation coaching</li>
 
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<li>Human Practices support</li>
 
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
 
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</ul>
 
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</div>
 
  
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=== Cloning ===
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* '''Ariana Natalie KLAPPERT''' <br />supported the cloning group during planning of restrictions, preparation of DNA, ligations and transformations. Ariana also worked on optimizing IPTG inducible protein expression in ''E. Coli''.
  
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<div class="clear"></div>
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* '''Caroline FANDREY''' <br />planned and executed restriction and ligation of genes, secretion tags and backbones and their transformation into ''E. coli'', and validated the results.
  
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<div class="column half_size">
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* '''BÙI Hoàng Duy''' <br />provided help in all tasks of cloning.
  
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<div class="highlight">
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* '''Jonas GOCKEL''' <br />took part in backbone cloning.
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<h5> Can we base our project on a previous one? </h5>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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* '''Laura LÓPEZ NAVARRETE''' <br />collaborated in assembling DNA constructs of tags and genes in ''E. coli'' and prepared rapid ligase buffer.
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</div>
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</div>
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* '''Michael TESKE''' <br />supported the team with restriction analysis, ligations, minipreps and prepared rapid ligase buffer.
  
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<div class="column half_size">
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* '''Niklas Arndt SCHMACKE''' <br />Designed the part sequences of nprb and sacB as well as the genes to be tested in deinking together with Max Schelski.
  
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<h5>Inspiration</h5>
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* '''Sophia MÄDLER''' <br />assembled our working backbone, took care of introducing the working methods to new members, designed the pLac-RBS construct, performed analysis of sequencing results and planned experiments to assemble all constructs.
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<p>Take a look at what other teams have done:</p>
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<ul>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
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</ul>
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</div>
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* '''Triinu LINKGREIM''' <br />took part in backbone cloning.
  
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<div class="clear"></div>
 
  
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<div class="column half_size">
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=== Deinking ===
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* '''Gintarė MALININAITĖ''' <br />was responsible for the deinking part of the project and took part in the design of the experiments.
  
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<h5>Team training and Project start</h5>
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* '''Stefan KOTTE''' <br />did a solvent analysis, established the grayscale analysis and refined the deinking process on the lab scale.
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
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<ul>
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
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<li>When did you start this course?</li>
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<li>Are the syllabus and course materials freely available online?</li>
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<li>When did you start your brainstorming?</li>
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<li>When did you start in the lab?</li>
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<li>When did you start working on  your project?</li>
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</ul>
 
  
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</div>
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=== Specific Enzyme Essays ===
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* '''Sophia MÄDLER''' <br />established all protocols to determine specific enzymatic activity of lipases, xylanases and endoglucanases in our lab.
  
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* '''Triinu LINKGREIM''' <br />refined the enzyme activity assays and was responsible for measuring enzymatic activity in the project.
  
  
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=== Human Practice ===
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* '''Alina SIEBENMORGEN''' <br />one of the main organizing personnel of Science Slam, participated in contacting sponsors and organized team events.
  
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</div>
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* '''Ariana Natalie KLAPPERT''' <br />worked on and held our science slam presentation.
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</html>
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* '''Benedikt HÖLBLING''' <br />one of the main organizing personnel of Science Slam, participated in contacting sponsors
 +
 
 +
* '''Caroline FANDREY''' <br />helped with our presentation for the science slam.
 +
 
 +
* '''BÙI Hoàng Duy''' <br />sponsoring delegate of the team.
 +
 
 +
* '''Gintarė MALININAITĖ''' <br />responsible for the teams sponsoring undertakings, contacted paper companies and helped with science slam organization.
 +
 
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* '''Jan Niklas HANSEN''' <br />co-organized Science Slam and provided contact with the CAESAR institute.
 +
 
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* '''Laura LÓPEZ NAVARRETE''' <br />wrote an essay about crucial factors in biosafety and social implications relevant to achieving success in projects like these.
 +
 
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* '''Michael TESKE''' <br />created and evaluated our survey about synthetic Biology.
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=== General Project ===
 +
* '''Balthasar SCHLOTMANN''' <br />gave advice and motivation to the team and represented the team in iGEM meet ups with holding presentations. Balthasar also participated in the development of the project idea.
 +
 
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* '''Niklas Arndt SCHMACKE''' <br />gave scientific advice, technical support and planned experiments for the cloning, ''B. Subtilis'' and deinking parts of the project.
 +
 
 +
* '''Sophia MÄDLER''' <br />was responsible for organizing the labwork and planning experiments throughout all areas. Sophia was involved in the development of the project idea.
 +
 
 +
 
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=== Wiki support ===
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* '''Balthasar SCHLOTMANN''' <br />created graphics for the wiki.
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* '''Michael TESKE''' <br />designed and programmed the wiki template. Michael organized the creation of the wiki.
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== Fallen Heroes ==
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<html><img src="//2016.igem.org/wiki/images/5/50/T--UBonn_HBRS--monument.svg" alt="Memorial" class="col-xs-4 col-md-2" style="float:right; height: 120px;"/></html>
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We would also like to thank people who are no longer involved in the project, but whose contribution was essential: Cathleen Hagemann, Mariya Chernyavska, Pavel Ryzhov, Max Schelski, Guido de Boer, Katrin Ciupka, Alena Sommer, Ashwin Shah, Clarissa Hiltl, Virginia Malchus and Paul Maier.
 +
 
 +
 
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== Human Practices support ==
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'''CAESAR''' <br />We thank the Center of Advanced European Studies and Research (caesar) for providing the venue for our science slams, contact to the public and especially Christian Siegel for creating, with a lot of expertise, the movies of the slams.
 +
 
 +
 
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== Fotos ==
 +
Our team's official photos were taken by Kim Hyuntae.
 +
 
 +
{{UBonn_HBRS/footer}}

Latest revision as of 16:22, 1 December 2016

 

Attributions

We are very thankful for the lots of help we received! Please also check out our great Sponsors, whom we dedicated a special page:
Our Sponsors


General support

We thank the Rheinische Friedrich-Wilhelms-University of Bonn, the Limes Institute Bonn and the University of Applied Sciences Bonn-Rhein-Sieg for providing the laboratory space, material and funding.


Scientific supervision

We are very grateful to Prof. Dr. Günther Mayer and Prof. Dr. Michael Famulok of University Bonn for supervising this project. We would also like to thank Prof. Dr. Klaus Lehmann of HBRS for his resourceful and unlimited support.


Lab support

Our dearest thanks go to our instructor Ignacio Geraci and all the other PhDs, PhD students and technicians of Professor Mayer’s and Prof. Famulok’s laboratory, especially Thinhinane Chaou, Laia Civit, Laura Eiden, Jeffrey Hannam, Mark Kerzhner, Daniel Matzner, Franziska Pfeiffer, Anna Pyka, Christian Renzl, Malte Rosenthal, Anna Schüller, Julia Siegl, Justina Stark, Jörg Ewers, Sebastian Pilsl, Silvana Haßel and Olga Wolter for their help and patience. We would also like to give special thanks to Stefan Künne for all his support and supervision throughout the project.

Heart


B. subtilis support

For their support with electroporation and the use of their equipment we would like to thank all members of the AG Dickschadt from the chemical institute of the University of Bonn. Furthermore, we would like to thank Prof. Satoshi Matsuoka for the gracious donation of B. subtilis expression plasmids containing three enzymes of interest and Prof. Thomas Schweder from the Institute of Pharmaceutical Biotechnology at the University of Greifswald for the use of two Bacillus subtilis strains LS8P-D and ATCC 6051.


Equipment Support

For the use of titration equipment we would like to thank Dr. Ralf Weisbarth from the Chemical Institute at the University of Bonn and for the use of a lipase kit we would like to thank Prof. Christoph Thiele from the LIMES institute at the University of Bonn.


Project advisor support

  • Benedikt HÖLBLING
    took care of the team’s finances, initiated and organized the team's science slams.
  • BÙI Hoàng Duy
    gave pieces of advice to the project.
  • Niklas Arndt SCHMACKE
    Was involved in the development of the project idea, introduced new team members to the theoretical and practical aspects of the project and iGEM and served as a remote advisor from Switzerland for a large part of the project.


Team Attributions

Our Team Members worked in the following groups, learn more about our team on our
Team Page


B. subtilis

  • Ariana Natalie KLAPPERT
    optimzied PAGE gel analysis and protein expression.
  • Jonas GOCKEL
    prepared competent bacteria, tested transformation protocols and established the Bacillus Subtilis collaboration with Freiburg.


Cloning

  • Ariana Natalie KLAPPERT
    supported the cloning group during planning of restrictions, preparation of DNA, ligations and transformations. Ariana also worked on optimizing IPTG inducible protein expression in E. Coli.
  • Caroline FANDREY
    planned and executed restriction and ligation of genes, secretion tags and backbones and their transformation into E. coli, and validated the results.
  • BÙI Hoàng Duy
    provided help in all tasks of cloning.
  • Jonas GOCKEL
    took part in backbone cloning.
  • Laura LÓPEZ NAVARRETE
    collaborated in assembling DNA constructs of tags and genes in E. coli and prepared rapid ligase buffer.
  • Michael TESKE
    supported the team with restriction analysis, ligations, minipreps and prepared rapid ligase buffer.
  • Niklas Arndt SCHMACKE
    Designed the part sequences of nprb and sacB as well as the genes to be tested in deinking together with Max Schelski.
  • Sophia MÄDLER
    assembled our working backbone, took care of introducing the working methods to new members, designed the pLac-RBS construct, performed analysis of sequencing results and planned experiments to assemble all constructs.
  • Triinu LINKGREIM
    took part in backbone cloning.


Deinking

  • Gintarė MALININAITĖ
    was responsible for the deinking part of the project and took part in the design of the experiments.
  • Stefan KOTTE
    did a solvent analysis, established the grayscale analysis and refined the deinking process on the lab scale.


Specific Enzyme Essays

  • Sophia MÄDLER
    established all protocols to determine specific enzymatic activity of lipases, xylanases and endoglucanases in our lab.
  • Triinu LINKGREIM
    refined the enzyme activity assays and was responsible for measuring enzymatic activity in the project.


Human Practice

  • Alina SIEBENMORGEN
    one of the main organizing personnel of Science Slam, participated in contacting sponsors and organized team events.
  • Ariana Natalie KLAPPERT
    worked on and held our science slam presentation.
  • Benedikt HÖLBLING
    one of the main organizing personnel of Science Slam, participated in contacting sponsors
  • Caroline FANDREY
    helped with our presentation for the science slam.
  • BÙI Hoàng Duy
    sponsoring delegate of the team.
  • Gintarė MALININAITĖ
    responsible for the teams sponsoring undertakings, contacted paper companies and helped with science slam organization.
  • Jan Niklas HANSEN
    co-organized Science Slam and provided contact with the CAESAR institute.
  • Laura LÓPEZ NAVARRETE
    wrote an essay about crucial factors in biosafety and social implications relevant to achieving success in projects like these.
  • Michael TESKE
    created and evaluated our survey about synthetic Biology.


General Project

  • Balthasar SCHLOTMANN
    gave advice and motivation to the team and represented the team in iGEM meet ups with holding presentations. Balthasar also participated in the development of the project idea.
  • Niklas Arndt SCHMACKE
    gave scientific advice, technical support and planned experiments for the cloning, B. Subtilis and deinking parts of the project.
  • Sophia MÄDLER
    was responsible for organizing the labwork and planning experiments throughout all areas. Sophia was involved in the development of the project idea.


Wiki support

  • Balthasar SCHLOTMANN
    created graphics for the wiki.
  • Michael TESKE
    designed and programmed the wiki template. Michael organized the creation of the wiki.


Fallen Heroes

Memorial We would also like to thank people who are no longer involved in the project, but whose contribution was essential: Cathleen Hagemann, Mariya Chernyavska, Pavel Ryzhov, Max Schelski, Guido de Boer, Katrin Ciupka, Alena Sommer, Ashwin Shah, Clarissa Hiltl, Virginia Malchus and Paul Maier.


Human Practices support

CAESAR
We thank the Center of Advanced European Studies and Research (caesar) for providing the venue for our science slams, contact to the public and especially Christian Siegel for creating, with a lot of expertise, the movies of the slams.


Fotos

Our team's official photos were taken by Kim Hyuntae.

© iGEM Team Bonn 2016 Top ↑