Difference between revisions of "Team:SCAU-China/Collaborations"

 
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Experiments"> Experiments </a></li>
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       <a href="https://2016.igem.org/Team:SCAU-China" class="brand-logo" data-beloworigin="true" data-constrainwidth="false" data-activates="dropdown7">&nbsp;HOME</a>
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      <a id="vecter"></a>
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      <li><a href="#vecter" class="on">Vecter Constraction</a></li>
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      <li><a href="#tissue">Tissue Culture</a></li>
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <div class="text">Fig.1 Not I digestion of constructs cotaining different numbers of genes.
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<a class="right brand-logo dropdown-button" data-beloworigin="true" data-constrainwidth="false" style="cursor:pointer"  data-activates="dropdown4">RESULTS</a>
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Not I is the acceptor vector (pYLTAC380GW plasmid) and lanes 3-6 are intermediate products created during the construction of our delivering vecter,pYLTAC380MF-BBPC. Bands with arrows are the trangenes release from vector backbone and length of corresponding genes are indicated at the paretheses.
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
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      <li><a href="#phenotype">Phenotype of seeds</a></li>
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      <li><a href="#hplc">HPLC</a></li>
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      <p><img src="https://static.igem.org/mediawiki/2016/0/07/Image014.jpg">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/b/ba/Image016.png">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/5/5b/Image015.png"></p><br>
 
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      <p><img src="https://static.igem.org/mediawiki/2016/7/77/Image020.jpg">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/2/28/Image022.png">&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2016/d/d3/Image018.png"></p>
 
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      <li><a href="#vecter">Vecter Constraction</a></li>
 
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      <div class="text">Fig.n HPLC analysis diagram of standard samples of astaxanthin(a);
 
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HPLC analysis diagram of  samples extract  from trangenes rice HG-380MF-BBPC (b);HPLC analysis diagram of  samples extract  from  HG-WT rice (c);<br><br>
 
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Compared with the standard samples with HPLC analysis diagram, you can see the sample extract  from rice and standard samples  have the same peak at the same time, show that the  transgenic rice seed which carry the four genes  expressed astaxanthin. Results indicate  that we are successful synthesize astaxanthin by rice endosperm synthetic .</div> 
 
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      <li><a href="#pcr">PCR and RT-PCR analysis</a></li>
 
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      <div class="img"><img src="https://static.igem.org/mediawiki/2016/9/94/Image032.png"></div>
 
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      <div class="text">Fig.Relative transcript levels of isoprenoid and carotenoid biosynthetic genes in rice at filling stage.qPCR analysis of the expression of CrtI,BKT,BHY,PSY, PDS, ZDS, ISO,BLCY,ELCY,HYD and rPSY in the rice of HG-WT, its transformants HG-BBPC-8-6-1,HG-BBPC-8-6-2,HG-BBPC-13-5-2,HG-BBPC-13-5-3,HG-BBPC-13-9-1and HG-BBPC-13-9-6 .</div> 
 
 
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    <div class="h1_font_size"style="margin-bottom:10px">Collaborations</div><div>
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<div class="p_font_size" style="text-indent:0em">
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Collaboration is core values of iGEM, we devote ourselves to promote the knowledge of synthetic biology and to expand the impact of iGEM in China. We introduced the purpose and overview of iGEM competition the students of Guangdong Experimental High School and helped them to set up a team named GDSYZX-United for the iGEM competition. During this project, we could give some guidance to their project. We also provided some plasmids to them. And we also sent two teammates (Wang Rong and Lin Yunping) to Guangdong Experimental High School for several months to be their art and modeling advisor to help them build web page and design poster. We held a meeting to communicate about project progress and team construction, sharing experience and learning from each other.</div>
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<div class="p_font_size">&nbsp;</div>
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Besides, we participated the gatherings of Central China iGEM Consortium in Peking and Canton to enhance the collaboration and interaction with other iGEM teams in China.
 
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Latest revision as of 03:26, 3 December 2016

SCAU

Collaborations
Collaboration is core values of iGEM, we devote ourselves to promote the knowledge of synthetic biology and to expand the impact of iGEM in China. We introduced the purpose and overview of iGEM competition the students of Guangdong Experimental High School and helped them to set up a team named GDSYZX-United for the iGEM competition. During this project, we could give some guidance to their project. We also provided some plasmids to them. And we also sent two teammates (Wang Rong and Lin Yunping) to Guangdong Experimental High School for several months to be their art and modeling advisor to help them build web page and design poster. We held a meeting to communicate about project progress and team construction, sharing experience and learning from each other.
 
 
Besides, we participated the gatherings of Central China iGEM Consortium in Peking and Canton to enhance the collaboration and interaction with other iGEM teams in China.