Difference between revisions of "Team:SCAU-China"

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<li><a href="https://2016.igem.org/Team:SCAU-China/Time_shaft">Time shaft</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Time_shaft">Time shaft</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Members">Members</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Members">Members</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Advisors">Advisors</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Advisors">Advisors</a></li>
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<li><a href="  https://2016.igem.org/Team:SCAU-China/Attributions">Attributions  </a></li>
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    <li><a href="  https://2016.igem.org/Team:SCAU-China/Attributions">Attributions  </a></li>
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<li><a href=" https://2016.igem.org/Team:SCAU-China/Collaborations">Collaborations</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Description">Description</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Description">Description</a></li>
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<li><a href=" https://2016.igem.org/Team:SCAU-China/Design">Design</a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Design">Design</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Basic_Part">Basic parts</a></li>
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<li><a href=" https://2016.igem.org/Team:SCAU-China/Experiments"> Experiments  </a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Experiments"> Experiments  </a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Proof">Proof</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Proof">Proof</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Demonstrate">Demonstrate</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Demonstrate">Demonstrate</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Model">Model</a></li>
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<li><a href=" https://2016.igem.org/Team:SCAU-China/Notebook">Notebook</a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Notebook">Notebook</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Gallery">Gallery</a></li>
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    <li><a href="https://2016.igem.org/Team: SCAU-China/Gallery">Gallery</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Protocol">Protocol</a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Safety">Safety</a></li>
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<li><a href="  https://2016.igem.org/Team:SCAU-China/HP/Silver">Silver</a></li>
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    <li><a href="  https://2016.igem.org/Team:SCAU-China/HP/Silver">Silver</a></li>
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<li><a href=" https://2016.igem.org/Team:SCAU-China/HP/Gold">Gold </a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/HP/Gold">Gold </a></li>
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<li><a href="https://2016.igem.org/Team:SCAU-China/Integrated_Practices">Integrated</a></li>
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    <li><a href="https://2016.igem.org/Team:SCAU-China/Integrated_Practices">Integrated</a></li>
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<li><a href=" https://2016.igem.org/Team:SCAU-China/Engagement">Engagement</a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Engagement">Engagement</a></li>
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<li><a href=" https://2016.igem.org/Team:SCAU-China/Entrepreneurship">Entrepreneurship</a></li>
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    <li><a href=" https://2016.igem.org/Team:SCAU-China/Entrepreneurship">Entrepreneurship</a></li>
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<a href="https://2016.igem.org/Team:SCAU-China" class="brand-logo" data-beloworigin="true" data-constrainwidth="false" data-activates="dropdown7">&nbsp;HOME</a>
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<a class="right brand-logo dropdown-button" data-beloworigin="true" data-constrainwidth="false" style="cursor:pointer"  data-activates="dropdown5">NOTE</a>
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<div class="h1_font_size">Experiement</div>
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<div class="h2_font_size"><font style="font-weight:bold">Vector construction</font></div>
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<div class="p_font_size">To synthesize astaxanthin and delete selective resistance gene, six gene expression cassettes were assembled into a TAC-based binary acceptor vector, designated as pYLTAC380MF-BBPC (Figure 1), by multiple rounds of gene assembly cycles using our marker-free TransGene Stacking II system, in which contained four genes (<font style="font-style:italic">CrtI, PSY, BKT </font>and <font style="font-style:italic">BHY</font>) under the control of 4 different endosperm-specific promoters for astaxanthin biosynthesis, and two genes (<font style="font-style:italic">HPT</font> and <font style="font-style:italic">Cre</font>) for marker-free deletion.</div>
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<div class="p_font_size" style="margin-bottom:20px"><small><font style="font-weight:bold">Figure 1:</font> Structure and restriction analysis of multigene recombinant constructs. Structure and restriction analysis of recombinant constructs. (A) Structure of a construct pYLTAC380MF-BBPC containing four genes for astaxanthin biosynthesis and two genes for marker free. The numbers in parentheses indicate the order of the sequences inserting into the vector. N denotes Not Isites. (B) Restriction analysis of a series of multigene constructs containing different numbers of genes (from lanes 3 to 6). Arrows indicate the different insertion genes.</small></div>
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<div class="h2_font_size"><font style="font-weight:bold">Rice transformation</font></div>
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<div class="p_font_size">The multigene construct pYLTAC380MF-BBPC was introduced into <font style="font-style:italic">Agrobacterium</font> tumefaciens strain <font style="font-style:italic">EHA105</font> by electroporation. Then the <font style="font-style:italic">Agrobacterium</font> cells were cocultured with embryogenic calli induced from mature seeds of Indica rice varieties Huaguang1 (HG1) as described (Lin et al., PNAS, 2003, 100: 5962-5967). Regenerating calli were selected in the presence of 50 mg/L hygromycin and subsequently transferred to rooting medium containing hygromycin. After further culturing for 3 to 4 weeks, transformed plants were transferred to soil in a greenhouse. Figure 2 showed the agrobacterium-mediated transformation process and transgenic rice cultivation. You can read more details, <a href="https://2016.igem.org/Team:SCAU-China/Protocol#rice_transformation" text-decoration=underline>please click here!</a></div><!--(加链接到Notebook栏目下面的3.protocol的2. rice transformation)-->
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        <div class="p_font_size"> Our team has been working on the iGEM-2016 project for two years. In this project, we used a crop plant rice (Oryza sativa L.) as chassis organism to produce a genetically modified organism (GMO) named aSTARice, which is able to produce astaxanthin in the rice endosperm. To achieve this goal, four genes derived from maize, algae, and bacteria respectively were expressed in transgenic rice driven by endosperm-specific promoters.  </div>
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        <div class="p_font_size">In the past two years, we have successfully held several public activities such as open laboratory day and a Bauhinia Scientific and Cultural Festival in our university, which attracted around hundreds and thousands of people to visit a laboratory of molecular biology, to understand how a GMO is generated in the lab. </div>
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        <div class="p_font_size">During the period of our project, we gave several biology courses for middle school students, kids and farmers on knowledge about modern agriculture in the ruler countryside in our province. Thereby, we promoted the knowledge of synthetic biology and impact of iGEM in human practices.</div>
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<div class="p_font_size" style="margin-bottom:20px"><small> <font style="font-weight:bold">Figure 2:</font><font style="font-style:italic"> Agrobacterium</font>-mediated rice calli transformation process.</small></div>
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<div class="p_font_size">To confirm the realization of our designed pathway, PCR amplification and semi-quantitative RT-RCR analysis were performed. The experimental results were presented in our <a href="https://2016.igem.org/Team:SCAU-China/Proof" text-decoration=underline>Proof page.</a></div><!--(超链接到 Proof).-->
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Revision as of 17:12, 14 October 2016

SCAU

Our team has been working on the iGEM-2016 project for two years. In this project, we used a crop plant rice (Oryza sativa L.) as chassis organism to produce a genetically modified organism (GMO) named aSTARice, which is able to produce astaxanthin in the rice endosperm. To achieve this goal, four genes derived from maize, algae, and bacteria respectively were expressed in transgenic rice driven by endosperm-specific promoters.
In the past two years, we have successfully held several public activities such as open laboratory day and a Bauhinia Scientific and Cultural Festival in our university, which attracted around hundreds and thousands of people to visit a laboratory of molecular biology, to understand how a GMO is generated in the lab.
During the period of our project, we gave several biology courses for middle school students, kids and farmers on knowledge about modern agriculture in the ruler countryside in our province. Thereby, we promoted the knowledge of synthetic biology and impact of iGEM in human practices.