Difference between revisions of "Team:HokkaidoU Japan/Overview"

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<p><div id="Project_Description"><img src="https://static.igem.org/mediawiki/2016/d/d2/T--HokkaidoU_Japan--project_description.png"  
 
<p><div id="Project_Description"><img src="https://static.igem.org/mediawiki/2016/d/d2/T--HokkaidoU_Japan--project_description.png"  
 
width="300px" height="110px" alt="Project description"></div></p>
 
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Hydrogel is used in contact lenses and diapers.
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<br>We considered possible application of self-assembling peptides (SAPs). SAPs have a self-assembling ability because they are amphiphilic peptides and contain hydrophobic and hydrophilic residues. These electric charged amino acids interact with one another to form spontaneously antiparallel β-sheet in a physiochemical environmental condition. </br>
Some kinds of hydrogel are made of self-assembling peptides.  
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<br>We use one of SAP, RADA-16-(RADARADARADARADA)and P11-4 (QQRFEWEFEQQ). RADA-16-I has been established for 3-D culture of neural stem cells (NSCs) by creating nanostructures. P11-4 has been designed to form fibers at low pH and have a cytocompatibility. </br>
They are amphiphilic peptides and they form nanostructured hydrogel under physiochemical conditions.
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<br>Recently, SAPs have been used for a nanostructured hydrogel. We considered new application methods of SAPs such as enhancement of enzymatic activity. For example, circulated enzymes offer a potential to stablize with heat and pH and multimeric one may be continuously reactive. </br>
Recently, they have been considered as a good biological scaffold for cell culturing and drug delivering.  
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They are synthesized chemically such as solid-state synthesis, but it’s not efficient for production in large amounts.
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</font></p>
So, we need another approach including biosynthesis.</p>  
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<br>In this research, we expressed self-assembling peptides such as
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<p><CENTER>RADA-16 I
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and P11-4</CENTER></p>
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in <i>Escherichia coli</i> and analyzed them by a series of experiments.
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<br>We also considered the application of hydrogel to immobilized proteins,
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which may enhance the stability of proteins.  
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Immobilized proteins will be used in medicine and environmental study in the future.</font></p>
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<p><div id="Background"><img src="https://static.igem.org/mediawiki/2016/1/1c/T--HokkaidoU_Japan--background.png"  
 
<p><div id="Background"><img src="https://static.igem.org/mediawiki/2016/1/1c/T--HokkaidoU_Japan--background.png"  

Revision as of 02:12, 15 October 2016

Team:HokkaidoU Japan - 2016.igem.org

 

Team:HokkaidoU Japan

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Project description


We considered possible application of self-assembling peptides (SAPs). SAPs have a self-assembling ability because they are amphiphilic peptides and contain hydrophobic and hydrophilic residues. These electric charged amino acids interact with one another to form spontaneously antiparallel β-sheet in a physiochemical environmental condition.

We use one of SAP, RADA-16-(RADARADARADARADA)and P11-4 (QQRFEWEFEQQ). RADA-16-I has been established for 3-D culture of neural stem cells (NSCs) by creating nanostructures. P11-4 has been designed to form fibers at low pH and have a cytocompatibility.

Recently, SAPs have been used for a nanostructured hydrogel. We considered new application methods of SAPs such as enhancement of enzymatic activity. For example, circulated enzymes offer a potential to stablize with heat and pH and multimeric one may be continuously reactive.


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