Difference between revisions of "Team:Waterloo/Parts"

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        This construct is a control to test the metabolic load of the Hsp104-CFP fusion protein and as a baseline against which we compare the change in [PSI+]/[psi-] state. We synthesized a fragment to clone into the [Hsp-PRS315]. This plasmid will be referred to as the Hsp104 plasmid and is used in several of our experiments.
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Asceptically pipette 5mL sterile liquid broth into a test tube. If there are no large pipette tips able to transfer 5mL of liquid broth, use a fresh falcon tube to measure.
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Add appropriate antibiotic, if required, to desired concentration using a pipette. You will have to tilt the test tube so that the broth is close enough to the top of the tube that you can add the antibiotic to the broth. Dropping it in won’t work because you will typically be adding ~2.5mL and the drop will be too small to fall.
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It is recommended that you make a “mastermix” with broth and antibiotics in it when you have many tubes that require the same antibiotic.
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Revision as of 19:41, 15 October 2016

Our System
Hsp104-NSC
Hsp104-SC1
Hsp104-SC2
Biobrick
ADH1-GFP
Gal1-GFP
Cup1-GFP
Gal1-CFP NSC
Gal1-CFP SC1
Gal1-CFP SC2
Promoter Testing
Hsp104-Gal1
Hsp104-Cup1
pXP218-ADH1
pXP218-Gal1
pXP218-Cup1
Hsp104-NSC
This construct is a control to test the metabolic load of the Hsp104-CFP fusion protein and as a baseline against which we compare the change in [PSI+]/[psi-] state. We synthesized a fragment to clone into the [Hsp-PRS315]. This plasmid will be referred to as the Hsp104 plasmid and is used in several of our experiments.