Difference between revisions of "Team:Waterloo/Parts"
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The Hsp104 plasmid. | The Hsp104 plasmid. | ||
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| − | <img src="https://static.igem.org/mediawiki/2016/a/aa/T--Waterloo--HSP104_LEU_Addgene_Annotate_and_CFP_Gal1. | + | <img src="https://static.igem.org/mediawiki/2016/a/aa/T--Waterloo--HSP104_LEU_Addgene_Annotate_and_CFP_Gal1.png" class="wcontent-img-solo37"> |
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The insertion of a Gal1,10 promoter and CFP fusion into the Hsp104 plasmid. | The insertion of a Gal1,10 promoter and CFP fusion into the Hsp104 plasmid. | ||
Revision as of 19:58, 15 October 2016
Hsp104-NSC
This construct is a control to test the metabolic load of the Hsp104-CFP fusion protein and as a baseline against which we compare the change in [PSI+]/[psi-] state. We synthesized a fragment to clone into the [Hsp-PRS315]. This plasmid will be referred to as the Hsp104 plasmid and is used in several of our experiments.
The Hsp104 plasmid.
The insertion of a Gal1,10 promoter and CFP fusion into the Hsp104 plasmid.
