Difference between revisions of "Team:Waterloo/Parts"

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<i>Our System</i>
 
<i>Our System</i>
 
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Revision as of 20:04, 15 October 2016

Our System
Hsp104-NSC
Hsp104-SC1
a href="" ng-click="active='SC2'">Hsp104-SC2
Biobrick
ADH1-GFP
Gal1-GFP
Cup1-GFP
Gal1-CFP NSC
Gal1-CFP SC1
Gal1-CFP SC2
Promoter Testing
Hsp104-Gal1
Hsp104-Cup1
pXP218-ADH1
pXP218-Gal1
pXP218-Cup1
Hsp104-NSC

This construct is a control to test the metabolic load of the Hsp104-CFP fusion protein and as a baseline against which we compare the change in [PSI+]/[psi-] state. We synthesized a fragment to clone into the [Hsp-PRS315]. This plasmid will be referred to as the Hsp104 plasmid and is used in several of our experiments.

The Hsp104 plasmid.
The insertion of a Gal1,10 promoter and CFP fusion into the Hsp104 plasmid.