Difference between revisions of "Team:Waterloo/Parts"
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<p class="paragraph-medium">This construct is a control to test the metabolic load of the Hsp104-CFP fusion protein and as a baseline against which we compare the change in [PSI+]/[psi-] state. We synthesized a fragment to clone into the [Hsp-PRS315]. This plasmid will be referred to as the Hsp104 plasmid and is used in several of our experiments.</p> | <p class="paragraph-medium">This construct is a control to test the metabolic load of the Hsp104-CFP fusion protein and as a baseline against which we compare the change in [PSI+]/[psi-] state. We synthesized a fragment to clone into the [Hsp-PRS315]. This plasmid will be referred to as the Hsp104 plasmid and is used in several of our experiments.</p> | ||
<img src="https://static.igem.org/mediawiki/2016/e/e6/T--Waterloo--HSP104_LEU_Addgene_Annotate.png" class="wcontent-img-solo37"> | <img src="https://static.igem.org/mediawiki/2016/e/e6/T--Waterloo--HSP104_LEU_Addgene_Annotate.png" class="wcontent-img-solo37"> | ||
| − | <div class="wcontent-caption"> | + | <div class="wcontent-caption paragraph-medium"> |
The Hsp104 plasmid. | The Hsp104 plasmid. | ||
</div> | </div> | ||
<img src="https://static.igem.org/mediawiki/2016/a/aa/T--Waterloo--HSP104_LEU_Addgene_Annotate_and_CFP_Gal1.png" class="wcontent-img-solo37"> | <img src="https://static.igem.org/mediawiki/2016/a/aa/T--Waterloo--HSP104_LEU_Addgene_Annotate_and_CFP_Gal1.png" class="wcontent-img-solo37"> | ||
| − | <div class="wcontent-caption"> | + | <div class="wcontent-caption paragraph-medium"> |
The insertion of a Gal1,10 promoter and CFP fusion into the Hsp104 plasmid. | The insertion of a Gal1,10 promoter and CFP fusion into the Hsp104 plasmid. | ||
</div> | </div> | ||
Revision as of 20:06, 15 October 2016
Hsp104-NSC
This construct is a control to test the metabolic load of the Hsp104-CFP fusion protein and as a baseline against which we compare the change in [PSI+]/[psi-] state. We synthesized a fragment to clone into the [Hsp-PRS315]. This plasmid will be referred to as the Hsp104 plasmid and is used in several of our experiments.
The Hsp104 plasmid.
The insertion of a Gal1,10 promoter and CFP fusion into the Hsp104 plasmid.
