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Revision as of 20:41, 15 October 2016

Lethbridge iGEM 2016



Nanoresponder

Single-domain antibody production.
Explore


Introduction

The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.

Dr. HJ Wiedenmore_vert
Dr. HJ Wiedenclose

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Dr. Brian Dempseymore_vert
Dr. Brian Dempseyclose

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Dr. Cesar Rodriguezmore_vert
Dr. Cesar Rodriguezclose

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Dr. Andrew Hudsonmore_vert
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Taylor Sheahanmore_vert
Taylor Sheahanclose

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Karin Oteromore_vert
Karin Oteroclose

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Rhys Hakstolmore_vert
Rhys Hakstolclose

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Rhys Hakstolmore_vert
Rhys Hakstolclose

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Suneet Khareymore_vert
Suneet Khareyclose

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Dustin Smithmore_vert
Dustin Smithclose

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Chris Isaacmore_vert
Chris Isaacclose

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Keith Aikenmore_vert
Keith Aikenclose

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Sydnee Calhounmore_vert
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Kieran McCormackmore_vert
Kieran McCormackclose

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Jeff Tingleymore_vert
Jeff Tingleyclose

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Kali Sage Strobelmore_vert
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Rebecca Jeongmore_vert
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I am a very simple card. I am good at containing small bits of information. I am convenient because I require little markup to use effectively. I am similar to what is called a panel in other frameworks.
Overview

The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.

Our Project
Human Practices