Difference between revisions of "Team:Lethbridge/HP/Gold"
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<li><a href="https://2016.igem.org/Team:Lethbridge/Description" style="color:#616161;">Description</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Description" style="color:#616161;">Description</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Design" style="color:#616161;">Design</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Design" style="color:#616161;">Design</a></li> | ||
| − | <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments" style="color:#616161;"> | + | <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments" style="color:#616161;">Experiment</a></li> |
| + | <li><a href="https://2016.igem.org/Team:Lethbridge/Database" style="color:#616161;">Database</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Notebook" style="color:#616161;">Notebook</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook" style="color:#616161;">Notebook</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Parts" style="color:#616161;">Parts</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Parts" style="color:#616161;">Parts</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Safety" style="color:#616161;">Safety</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Safety" style="color:#616161;">Safety</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:Lethbridge/RNAi" style="color:#616161;">RNAi</a></li> | ||
</ul> | </ul> | ||
<li><a class="dropdown-button" href="#!" data-activates="dropdown2" style="display: block; padding-top: 19px; padding-bottom: 19px">Practices</a></li> | <li><a class="dropdown-button" href="#!" data-activates="dropdown2" style="display: block; padding-top: 19px; padding-bottom: 19px">Practices</a></li> | ||
<ul id='dropdown2' class='dropdown-content' style="list-style-image: none; margin-left: 0px; width: auto;"> | <ul id='dropdown2' class='dropdown-content' style="list-style-image: none; margin-left: 0px; width: auto;"> | ||
| − | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#intro" style="color:#616161;"> | + | <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices" style="color:#616161;">Integrated Practices</a></li> |
| + | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#intro" style="color:#616161;">Contact</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#interviews" style="color:#616161;">Interviews</a></li><!--NEEDS STUFF--> | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#interviews" style="color:#616161;">Interviews</a></li><!--NEEDS STUFF--> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#litrev" style="color:#616161;">Literature Review</a></li><!--NEEDS STUFF--> | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#litrev" style="color:#616161;">Literature Review</a></li><!--NEEDS STUFF--> | ||
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<li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#protocol" style="color:#616161;">Protocol</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#protocol" style="color:#616161;">Protocol</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#sampling" style="color:#616161;">Sampling</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#sampling" style="color:#616161;">Sampling</a></li> | ||
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</ul> | </ul> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Results" style="display: block; padding-top: 19px; padding-bottom: 19px">Results</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Results" style="display: block; padding-top: 19px; padding-bottom: 19px">Results</a></li> | ||
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<ul id='dropdown3' class="dropdown-content" style="list-style-image: none; margin-left: 0px; width: auto;"> | <ul id='dropdown3' class="dropdown-content" style="list-style-image: none; margin-left: 0px; width: auto;"> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Team" style="color:#616161;">Members</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Team" style="color:#616161;">Members</a></li> | ||
| − | <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations" style="color:#616161;"> | + | <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations" style="color:#616161;">Collab</a></li> |
<li><a href="https://2016.igem.org/Team:Lethbridge/Attributions" style="color:#616161;">Attribution</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions" style="color:#616161;">Attribution</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors" style="color:#616161;">Sponsors</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors" style="color:#616161;">Sponsors</a></li> | ||
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<li><a href="https://2016.igem.org/Team:Lethbridge/Design">Design</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Design">Design</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Experiments">Experiments</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Experiments">Experiments</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:Lethbridge/Database">Database</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Notebook">Notebook</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Notebook">Notebook</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Parts">Parts</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Parts">Parts</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Safety">Safety</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Safety">Safety</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:Lethbridge/RNAi">RNAi</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| + | <li><a href="https://2016.igem.org/Team:Lethbridge/Integrated_Practices">Integrated Practices</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#intro">Intro</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#intro">Intro</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#interviews">Interviews</a></li><!--NEEDS STUFF--> | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Silver#interviews">Interviews</a></li><!--NEEDS STUFF--> | ||
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<li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#protocol">Protocol</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#protocol">Protocol</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#sampling">Sampling</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/HP/Gold#sampling">Sampling</a></li> | ||
| − | + | </ul> | |
</div> | </div> | ||
</li> | </li> | ||
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<ul> | <ul> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Team">Members</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Team">Members</a></li> | ||
| − | <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations"> | + | <li><a href="https://2016.igem.org/Team:Lethbridge/Collaborations">Collaborations</a></li> |
<li><a href="https://2016.igem.org/Team:Lethbridge/Attributions">Attribution</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Attributions">Attribution</a></li> | ||
<li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li> | <li><a href="https://2016.igem.org/Team:Lethbridge/Sponsors">Sponsors</a></li> | ||
Revision as of 03:48, 19 October 2016
Human Practices (Gold)
Ridealongs, Protocol Design, Ambulance Sampling
Ridealongs
The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.
Protocol Design
The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.
Ambulance Sampling
The prevalence of virulent and multiple antibiotic resistant pathogens in healthcare facilities has resulted in ongoing reassessment of best practices to prevent their transmission. However, whether pathogen reservoirs exist in emergency medical services (EMS) vehicles, remains largely unknown. Our iGEM team developed a custom bacterial two hybrid system to select single-domain antibodies (nanobodies) that recognize human pathogens. Informed by our Nanopore next generation sequencing of DNA samples from ambulance vehicles, antibodies targeting important pathogens were then used to develop a rapid and low cost ELISA-based testing kit that may be employed on-site by EMS workers. Our project provides a framework for rapid detection of emergent pathogens and a practical and rapid solution for monitoring their presence in and outside of the healthcare system.
