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</center> | </center> | ||
<br clear="all"> | <br clear="all"> | ||
− | + | </div> | |
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<br> | <br> | ||
The following Table2 is about preparing the SDS-PAGE. | The following Table2 is about preparing the SDS-PAGE. | ||
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</table> | </table> | ||
+ | </center> | ||
+ | |||
<br> | <br> | ||
After we finished cultivating ?samples, we took 100μl out of each samples and made the following operation. | After we finished cultivating ?samples, we took 100μl out of each samples and made the following operation. | ||
+ | <br> | ||
+ | <ol> | ||
+ | <li>Centrifuge with 13000rpm at 24°C for 2min</li> | ||
+ | <li>Remove the supernatant</li> | ||
+ | <li>Add 50 mL of SDS-Buffer</li> | ||
+ | <li>Shake for 1 min</li> | ||
+ | <li>Keep at 100°C for 5 min</li> | ||
+ | <li>Put on the ice</li> | ||
+ | <li>Apply 10 µ to SDS</li> | ||
+ | <li>Run electrophoresis for 1.5 h</li> | ||
+ | <li>Wash out with MiliQ</li> | ||
+ | <li>Shake at 24°C with 34 rpm for 1 h</li> | ||
+ | <li>Dye with QuickCBB</li> | ||
+ | </ol> | ||
− | + | <br> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
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The fig. 1 shows the result of SDS-PAGE. | The fig. 1 shows the result of SDS-PAGE. |
Revision as of 12:53, 18 October 2016
Team:HokkaidoU Japan
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We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on (pSB1C3)of E.coli(DH5α). At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
The following Table2 is about preparing the SDS-PAGE.
We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on (pSB1C3)of E.coli(DH5α). At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
Table. 1. Gel assay |
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The following Table2 is about preparing the SDS-PAGE.
Table. 2. Experimental condition |
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After we finished cultivating ?samples, we took 100μl out of each samples and made the following operation.
The fig. 1 shows the result of SDS-PAGE. |