Difference between revisions of "Team:UBonn HBRS/Description/Notebook/2016"

Line 20: Line 20:
 
Deinking:
 
Deinking:
 
*Measurement of inkjet absorbance spectrum
 
*Measurement of inkjet absorbance spectrum
−
*filtration-based method
+
*filtration-based deinking setup
  
  
Line 26: Line 26:
  
 
repetition of restriction analysis of all genes and gel clean ups of the genes.
 
repetition of restriction analysis of all genes and gel clean ups of the genes.
 +
 +
Deinking:
 +
inkjet ink absorbance spectrum and pulp preparation
 +
  
 
==13KW (28.3-3.4)==
 
==13KW (28.3-3.4)==
Line 43: Line 47:
 
*nprb + CpCel9
 
*nprb + CpCel9
 
*nprb + BsCel5 in C3
 
*nprb + BsCel5 in C3
 +
 +
Deinking:
 +
establishing the set up and starting experiments with different conc of NaOH and Na2SiO3
 +
 +
 +
==14KW (4.4-10.4)==
 +
Deinking:
 +
Experiments with different concentrations of surfactant oleic acid
 +
 +
 +
==15KW (11.4-17.4)==
 +
Deinking:
 +
experiments with different incubation time, vortexing time, temperature
 +
 +
 +
 +
==16KW (18.4-24.4)==
 +
Deinking:
 +
tests with solvents to dissolve ink and fibers
 +
 +
 +
==17KW (25.4-1.5)==
 +
Deinking:
 +
grayscale tests, solvent tests
 +
 +
 +
==18KW (2.5-8.5)==
 +
Deinking:
 +
pulp preparation
 +
 +
 +
==19KW (9.5-15.5)==
 +
Deinking:
 +
establishing water and standard chemical controls
 +
 +
 +
==20KW (16.5-22.5)==
 +
Deinking:
 +
establishing water and standard chemical controls
 +
  
 
==21KW (23.5-29.5)==
 
==21KW (23.5-29.5)==
Line 54: Line 98:
 
*nprb + EstC2 in C3
 
*nprb + EstC2 in C3
  
−
==22KW (30.6-5.6)==
+
Deinking:
 +
establishing oleic acid and oleic acid + citrate buffer controls
 +
 
 +
 
 +
==22KW (30.5-5.6)==
  
 
Restriction analysis of samples of the 21KW
 
Restriction analysis of samples of the 21KW
 +
Deinking:
 +
discovery: paper discs with CB turn red, tests to find the reason
 +
  
 
==23KW (6.6-12.6)==
 
==23KW (6.6-12.6)==
  
 
Inoculation of all genes from glycerol stocks and miniprep. Restriction analysis and gel clean up of the genes, ligation of the missing genes with C3.
 
Inoculation of all genes from glycerol stocks and miniprep. Restriction analysis and gel clean up of the genes, ligation of the missing genes with C3.
 +
Deinking:
 +
tests with oleic acid and oleic acid + cb controls
 +
  
 
==24KW (13.6-19.6)==
 
==24KW (13.6-19.6)==
Line 68: Line 122:
 
Ligation of genes and tags in C5.
 
Ligation of genes and tags in C5.
 
Minipreps of all of the samples.
 
Minipreps of all of the samples.
 +
Deinking:
 +
tests with shaking during incubation and different incubation time
 +
  
 
==25KW (20.6-26.6)==
 
==25KW (20.6-26.6)==
Line 73: Line 130:
 
Restriction analysis of the constructs
 
Restriction analysis of the constructs
 
Repetition of ligation of genes in C5 and transformation and preps.
 
Repetition of ligation of genes in C5 and transformation and preps.
 +
Deinking:
 +
rotation evaporator concentrating ink
 +
  
 
==26KW (27.6 - 3.7)==
 
==26KW (27.6 - 3.7)==
 
Restriction analysis of the mini preps.
 
Restriction analysis of the mini preps.
  
−
==28KW (11.7-7.17)==
+
 
 +
==28KW (11.7-17.7)==
 
Repetion of Ligations of genes and C5
 
Repetion of Ligations of genes and C5
 +
Deinking:
 +
flotation-based deinking, pulp preparation
 +
 +
==29KW (18.7-24.7)==
 +
Deinking:
 +
separation of ink from fibers in organic phase of big scale deinking, flotation-based deinking, foam collection, defoaming
 +
 +
 +
 +
==30KW (25.7-31.7)==
 +
Deinking:
 +
tests with toluene flotation-based deinking
 +
  
 
==31KW (1.8-7.8)==
 
==31KW (1.8-7.8)==
Line 85: Line 159:
 
Ligation of genes in C3 and genes + tags + C5
 
Ligation of genes in C3 and genes + tags + C5
 
transformation, and inoculation of liquid cultures of the above.
 
transformation, and inoculation of liquid cultures of the above.
 +
  
 
==32KW (8.8 - 14.8)==
 
==32KW (8.8 - 14.8)==
Line 94: Line 169:
 
Ligations of genes plus tags in bb_MCS
 
Ligations of genes plus tags in bb_MCS
 
Transformation of these samples
 
Transformation of these samples
 +
  
 
==33KW (15.8 - 21.8)==
 
==33KW (15.8 - 21.8)==
Line 104: Line 180:
 
Transformation and inoculation of the samples.
 
Transformation and inoculation of the samples.
 
mini preps
 
mini preps
 +
Deinking:
 +
creation of flat paper-like discs
 +
  
 
==34KW (22.8 - 28.8)==
 
==34KW (22.8 - 28.8)==
  
 
Restrictions of mini prep and repetition of mini prep
 
Restrictions of mini prep and repetition of mini prep
 +
 +
Deinking:
 +
grayscale tests with officer scanner and odisey scanner
 +
 +
 +
==35KW (29.8 - 4.9)==
 +
 +
==36KW (5.8 - 11.8)==
 +
Deinking:
 +
tests of water controls
 +
 +
 +
==37KW (12.9 - 18.9)==
 +
Deinking:
 +
scanning tests, different conditions tests
 +
 +
 +
==38KW (19.9 - 25.9)==
 +
==39KW (26.8 - 2.10)==
 +
Deinking:
 +
establishing water and standard controls into setup with kitchen mixer
 +
 +
 +
==40KW (3.10 - 9.10)==
 +
Deinking:
 +
xylanase started to test + cb
 +
 +
 +
==41KW (10.10 - 16.10)==
 +
Deinking:
 +
xyl tests + cb
 +
 +
 
{{UBonn_HBRS/footer}}
 
{{UBonn_HBRS/footer}}

Revision as of 00:42, 19 October 2016

 

Lab Notebook 2016

10KW (7.3-13.3)

transformation of genes bought at genescript: XynA, XynB, LipA, EstC2, CpCel9, CpCel5c, EngB, CelA, BsCel5, Bpul and Linkers: nprb, SacB inoculation of liquid cultures and restriction Analysi of linkers (SacB and nprb).

repetition of the transformation for XynB, EngB and linkers. Subsequently inoculation and miniprep of the samples. Preparation of glycerol stock.

restriction analysis of the genes.


11KW (14.3-20.3)

Repetion of restriction analysis from the genes KW10 and gel clean ups of the linkers.

Deinking:

  • Measurement of inkjet absorbance spectrum
  • filtration-based deinking setup


12KW (21.3-27.3)

repetition of restriction analysis of all genes and gel clean ups of the genes.

Deinking: inkjet ink absorbance spectrum and pulp preparation


13KW (28.3-3.4)

Restrictions and gel clean ups of all genes genes for a new gel clean up, because the ones of KW13 did not works. Ligation of genes from the gel clean ups into C3 and inoculation of cultures and minipreps. (1.04.16 :) -> successful ligation of

  • XynA
  • CelA
  • LipA
  • Bpul
  • CpCel5c
  • BsCel5
  • EstC2
  • EngB in C3
  • nprb + CpCel9
  • nprb + BsCel5 in C3

Deinking: establishing the set up and starting experiments with different conc of NaOH and Na2SiO3


14KW (4.4-10.4)

Deinking: Experiments with different concentrations of surfactant oleic acid


15KW (11.4-17.4)

Deinking: experiments with different incubation time, vortexing time, temperature


16KW (18.4-24.4)

Deinking: tests with solvents to dissolve ink and fibers


17KW (25.4-1.5)

Deinking: grayscale tests, solvent tests


18KW (2.5-8.5)

Deinking: pulp preparation


19KW (9.5-15.5)

Deinking: establishing water and standard chemical controls


20KW (16.5-22.5)

Deinking: establishing water and standard chemical controls


21KW (23.5-29.5)

Restriction analysis of genes from the 13KW and mini prep from samples of the 13KW. (28.05.16) successful ligation of:

  • nprb and sacB in C3
  • nprb + EstC2 in C3

Deinking: establishing oleic acid and oleic acid + citrate buffer controls


22KW (30.5-5.6)

Restriction analysis of samples of the 21KW Deinking: discovery: paper discs with CB turn red, tests to find the reason


23KW (6.6-12.6)

Inoculation of all genes from glycerol stocks and miniprep. Restriction analysis and gel clean up of the genes, ligation of the missing genes with C3. Deinking: tests with oleic acid and oleic acid + cb controls


24KW (13.6-19.6)

Restriction of bbMCS and gel clean ups. Ligations of genes in C5 and C3 Ligation of genes and tags in C5. Minipreps of all of the samples. Deinking: tests with shaking during incubation and different incubation time


25KW (20.6-26.6)

Restriction analysis of the constructs Repetition of ligation of genes in C5 and transformation and preps. Deinking: rotation evaporator concentrating ink


26KW (27.6 - 3.7)

Restriction analysis of the mini preps.


28KW (11.7-17.7)

Repetion of Ligations of genes and C5 Deinking: flotation-based deinking, pulp preparation

29KW (18.7-24.7)

Deinking: separation of ink from fibers in organic phase of big scale deinking, flotation-based deinking, foam collection, defoaming


30KW (25.7-31.7)

Deinking: tests with toluene flotation-based deinking


31KW (1.8-7.8)

Ligation of Fragment synthesized at IDT and C3 Ligation of genes in C3 and genes + tags + C5 transformation, and inoculation of liquid cultures of the above.


32KW (8.8 - 14.8)

Ligation of IDT and C3 Transformation and inoculation. Minipreps of samples and restriction analysis

Ligations of genes plus tags in bb_MCS Transformation of these samples


33KW (15.8 - 21.8)

Inoculation and miniprep Repetion of ligation of IDT and C3 Repetion of ligation of bb + nprb + genes Midi prep of SacB, bb and nprb Ligation of Genes + C5 and genes plus tags in C5. Transformation and inoculation of the samples. mini preps Deinking: creation of flat paper-like discs


34KW (22.8 - 28.8)

Restrictions of mini prep and repetition of mini prep

Deinking: grayscale tests with officer scanner and odisey scanner


35KW (29.8 - 4.9)

36KW (5.8 - 11.8)

Deinking: tests of water controls


37KW (12.9 - 18.9)

Deinking: scanning tests, different conditions tests


38KW (19.9 - 25.9)

39KW (26.8 - 2.10)

Deinking: establishing water and standard controls into setup with kitchen mixer


40KW (3.10 - 9.10)

Deinking: xylanase started to test + cb


41KW (10.10 - 16.10)

Deinking: xyl tests + cb


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