Difference between revisions of "Team:Michigan/Basic Part"
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<h1 style="text-align:center; font-size: 50px;"> <font face= "Poiret One">BBa_K2105000</h2> | <h1 style="text-align:center; font-size: 50px;"> <font face= "Poiret One">BBa_K2105000</h2> | ||
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| − | <img src="https://static.igem.org/mediawiki/2016/d/ | + | <img src="https://static.igem.org/mediawiki/2016/d/d2/033_Cropped.png" width="500" height="400" align="right" style="margin-right: 30px;"> |
<p style="text-align:center; font-size:20px;"><font face="verdana">The lacZ delta M15 mutation encodes a form of beta-galactosidase lacking residues 11-41. This mutation causes it to be inactive until complemented by the lacZ alpha fragment. The lacZ delta M15 mutant is constitutively expressed in DH5 alpha cells. When the delta M15 mutant and the alpha fragment undergo alpha complementation, they are able to cleave the substrate X-gal to produce a blue product.<br><br> | <p style="text-align:center; font-size:20px;"><font face="verdana">The lacZ delta M15 mutation encodes a form of beta-galactosidase lacking residues 11-41. This mutation causes it to be inactive until complemented by the lacZ alpha fragment. The lacZ delta M15 mutant is constitutively expressed in DH5 alpha cells. When the delta M15 mutant and the alpha fragment undergo alpha complementation, they are able to cleave the substrate X-gal to produce a blue product.<br><br> | ||
| − | <a href="http://parts.igem.org/Part: | + | <a href="http://parts.igem.org/Part:BBa_E0033"> Registry of Standard Biological Parts</a></p> |
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Revision as of 23:59, 18 October 2016
Basic Parts
BBa_K2105000
The lacZ delta M15 mutation encodes a form of beta-galactosidase lacking residues 11-41. This mutation causes it to be inactive until complemented by the lacZ alpha fragment. The lacZ delta M15 mutant is constitutively expressed in DH5 alpha cells. When the delta M15 mutant and the alpha fragment undergo alpha complementation, they are able to cleave the substrate X-gal to produce a blue product.
Registry of Standard Biological Parts
Registry of Standard Biological Parts
BBa_K2105000
The lacZ delta M15 mutation encodes a form of beta-galactosidase lacking residues 11-41. This mutation causes it to be inactive until complemented by the lacZ alpha fragment. The lacZ delta M15 mutant is constitutively expressed in DH5 alpha cells. When the delta M15 mutant and the alpha fragment undergo alpha complementation, they are able to cleave the substrate X-gal to produce a blue product.
Registry of Standard Biological Parts
Registry of Standard Biological Parts
