Difference between revisions of "Team:Michigan/Parts"

Line 1: Line 1:
−
{{Michigan}}
+
{{Team:Michigan/navbar-Irina}}
 +
{{Team:Michigan/Navigation}}
 +
 
 
<html>
 
<html>
 +
<head>
 +
    <link href="https://static.igem.org/mediawiki/2016/2/2f/Bootstrap-msbt-css.txt" rel="stylesheet" type="text/css"/>
 +
</head>
 +
<style>
  
−
<groupparts>
+
#sideMenu, #top_title {display:none;}
 +
#content { padding:0px; width:100%;}
 +
body {background-color:white; width:100%}
 +
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 +
#top_menu_inside, #top_menu_14 { height:20px;}
 +
  
  
  
 +
div.blue {
 +
    background-color: white;
 +
    color: white;
 +
    margin: 20px 0 20px 0;
 +
    padding: 20px;
 +
    border-size: 10px;
 +
    border-color:#00274c;
 +
    border-style: solid;
 +
    display: flex;
 +
}
  
−
<div class="column full_size">
+
div.maize {
 +
  background-color: white;
 +
  color: white;
 +
  margin: 20px 0 20px 0;
 +
  padding: 20px;
 +
  border-size: 10px;
 +
  border-color:#ffd700;
 +
  border-style:solid;
 +
  display: flex;
  
  
−
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
}
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
h2 {
 +
        font-family: 'Poiret One', cursive;
 +
        font-size: 30px;
 +
        color: black;
 +
        display: flex;
 +
      }
 +
h1 {
 +
        font-family: 'Poiret One', cursive;
 +
        font-size: 40px;
 +
        color: black;
 +
    }
 +
p {
 +
        font-family: 'Poiret One', cursive;
 +
        font-size: 30px;
 +
        color: black;
 +
        display: flex;
 +
      }
 +
div.container {
 +
  padding-top: 50px;
 +
}
 +
img {
 +
  display: block;
 +
  margin-left: auto;
 +
  margin-right: auto;
  
 +
}
  
−
</div>
+
div.col-md-3{
 +
  display: flex;
 +
 
 +
}
  
−
 
+
</style>
−
 
+
<body>
−
 
+
  <div class = "container"> <h1 style="text-align:center; font-size: 75px;"><font face= "Poiret One">Basic Parts</font></h1>
−
 
+
    <div class= "row">
−
<div class="column half_size">
+
    <div class = "blue">  
−
<div class="highlight">
+
      <div class = "col-md-12">
−
<h5>Note</h5>
+
        <h1 style="text-align:center; font-size: 50px;"> <font face= "Poiret One">BBa_K2105000 (New Part)</h2>
−
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
+
<br>
 +
        <img src="https://static.igem.org/mediawiki/2016/d/d9/PSB1C3_Cropped.png" width="500" height="400" align="left" style="margin-right: 30px;">
 +
<p style="text-align:center; font-size:20px;"><font face="verdana">The lacZ delta M15 mutation encodes a form of beta-galactosidase lacking residues 11-41. This mutation causes it to be inactive until complemented by the lacZ alpha fragment. The lacZ delta M15 mutant is constitutively expressed in DH5 alpha cells. When the delta M15 mutant and the alpha fragment undergo alpha complementation, they are able to cleave the substrate X-gal to produce a blue product. In our project, the delta M15 mutant is constitutively produced by the cell-free expression system, but the blue output is not produced unless the alpha fragment's transcription is activated in the presence of the target biomarker.<br><br>
 +
<a href="http://parts.igem.org/Part:BBa_K2105000"> Registry of Standard Biological Parts Entry</a></p>
 +
    </div>
 +
  </div>
 
</div>
 
</div>
 +
<div class= "row">
 +
    <div class = "maize">
 +
      <div class = "col-md-12">
 +
        <h1 style="text-align:center; font-size: 50px;"> <font face= "Poiret One">BBa_E0033 (New Application of Existing Part)</h2>
 +
<br>
 +
        <img src="https://static.igem.org/mediawiki/2016/d/d2/033_Cropped.png" width="500" height="400" align="right" style="margin-right: 30px;">
 +
<p style="text-align:center; font-size:20px;"><font face="verdana">The lacZ alpha fragment can be used to complement the delta M15 mutant to produce a colorimetric output by cleavage of X-gal. In our system, we use a single stranded version of this biobrick which has been cleaved in the middle of the coding sequence. This prevents transcription of the alpha fragment until a proximity dependent ligation reaction has occurred. Because the rate of this ligation reaction is drastically increased in the presence of a target protein biomarker, transcription of the lacZ alpha fragment only occurs when the biomarker is present.<br><br>
 +
<a href="http://parts.igem.org/Part:BBa_E0033"> Registry of Standard Biological Parts Entry</a></p>
 +
    </div>
 +
  </div>
 
</div>
 
</div>
−
 
−
 
−
 
−
 
−
<div class="column half_size">
 
−
 
−
<h5>Adding parts to the registry</h5>
 
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
 
</div>
 
</div>
−
 
+
</body>
−
 
+
−
 
+
−
 
+
−
 
+
−
<div class="column half_size">
+
−
 
+
−
<h5>What information do I need to start putting my parts on the Registry?</h5>
+
−
<p>The information needed to initially create a part on the Registry is:</p>
+
−
<ul>
+
−
<li>Part Name</li>
+
−
<li>Part type</li>
+
−
<li>Creator</li>
+
−
<li>Sequence</li>
+
−
<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
−
 
+
−
<p>
+
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
−
 
+
−
</div>
+
−
 
+
−
 
+
−
<div class="column half_size">
+
−
 
+
−
<h5>Inspiration</h5>
+
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
−
 
+
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
+
−
<ul>
+
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
+
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
+
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
−
</ul>
+
−
</div>
+
−
 
+
−
<div class="column full_size">
+
−
<h5>Part Table </h5>
+
−
<div class="highlight">
+
−
 
+
−
 
+
−
</html>
+
−
<groupparts>iGEM2016 Example</groupparts>
+
−
<html>
+
−
</div>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
 
+
 
</html>
 
</html>

Revision as of 01:53, 19 October 2016

Home Project Modeling Team BioBrick Human Practices Safety Awards

Basic Parts

BBa_K2105000 (New Part)


The lacZ delta M15 mutation encodes a form of beta-galactosidase lacking residues 11-41. This mutation causes it to be inactive until complemented by the lacZ alpha fragment. The lacZ delta M15 mutant is constitutively expressed in DH5 alpha cells. When the delta M15 mutant and the alpha fragment undergo alpha complementation, they are able to cleave the substrate X-gal to produce a blue product. In our project, the delta M15 mutant is constitutively produced by the cell-free expression system, but the blue output is not produced unless the alpha fragment's transcription is activated in the presence of the target biomarker.

Registry of Standard Biological Parts Entry

BBa_E0033 (New Application of Existing Part)


The lacZ alpha fragment can be used to complement the delta M15 mutant to produce a colorimetric output by cleavage of X-gal. In our system, we use a single stranded version of this biobrick which has been cleaved in the middle of the coding sequence. This prevents transcription of the alpha fragment until a proximity dependent ligation reaction has occurred. Because the rate of this ligation reaction is drastically increased in the presence of a target protein biomarker, transcription of the lacZ alpha fragment only occurs when the biomarker is present.

Registry of Standard Biological Parts Entry