Difference between revisions of "Team:Wageningen UR/Notebook/OptogeneticKillSwitch"
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Both MazE and MazF have been registered as BioBricks (parts <a href="http://parts.igem.org/Part:BBa_K1096001">BBa_K1096001</a> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K302033">BBa_K302033</a>, respectively). However, as of the creation of the kill switch, neither are available from the iGEM registry. For this reason, we chose to isolate both genes directly from <i>E. coli</i> genomic DNA via PCR. In addition, the following BioBricks were prepared from iGEM registry kits: | Both MazE and MazF have been registered as BioBricks (parts <a href="http://parts.igem.org/Part:BBa_K1096001">BBa_K1096001</a> and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K302033">BBa_K302033</a>, respectively). However, as of the creation of the kill switch, neither are available from the iGEM registry. For this reason, we chose to isolate both genes directly from <i>E. coli</i> genomic DNA via PCR. In addition, the following BioBricks were prepared from iGEM registry kits: | ||
<br> | <br> | ||
| + | <p> | ||
| + | <ul> | ||
<li><a href="http://parts.igem.org/Part:BBa_K592006">BBa_K592006</a>: FixK2 (Promoter triggered by YF1-FixJ)</li> | <li><a href="http://parts.igem.org/Part:BBa_K592006">BBa_K592006</a>: FixK2 (Promoter triggered by YF1-FixJ)</li> | ||
<li><a href="http://parts.igem.org/Part:BBa_K592016">BBa_K592016</a>: YF1-FixJ (Light sensor)</li> | <li><a href="http://parts.igem.org/Part:BBa_K592016">BBa_K592016</a>: YF1-FixJ (Light sensor)</li> | ||
<li><a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>: Double terminator</li> | <li><a href="http://parts.igem.org/Part:BBa_B0015">BBa_B0015</a>: Double terminator</li> | ||
<li><a href="http://parts.igem.org/Part:BBa_J23108">BBa_J23108</a>: Constituitive promoter of the Anderson family (Mid-level expression strength, arbitrarily chosen.)</li> | <li><a href="http://parts.igem.org/Part:BBa_J23108">BBa_J23108</a>: Constituitive promoter of the Anderson family (Mid-level expression strength, arbitrarily chosen.)</li> | ||
| + | </ul> | ||
</p> | </p> | ||
Revision as of 08:55, 19 October 2016
These experiments were performed primarily by Marijn, with significant contributions by Mark during the final phase. Unless otherwise indicated, protocols were done according to the Protocols page.
May 16 - June 6: Obtaining Parts
Both MazE and MazF have been registered as BioBricks (parts BBa_K1096001 and BBa_K302033, respectively). However, as of the creation of the kill switch, neither are available from the iGEM registry. For this reason, we chose to isolate both genes directly from E. coli genomic DNA via PCR. In addition, the following BioBricks were prepared from iGEM registry kits:
- BBa_K592006: FixK2 (Promoter triggered by YF1-FixJ)
- BBa_K592016: YF1-FixJ (Light sensor)
- BBa_B0015: Double terminator
- BBa_J23108: Constituitive promoter of the Anderson family (Mid-level expression strength, arbitrarily chosen.)
4 July - 1 August: pDawn Construction
8 August - 3 October: pDusk Construction
