Difference between revisions of "Team:TU Darmstadt/Notebook"

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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>Decision who will participate in the project and which direction we chose.
 +
4 people forming the team and we do some research.
 +
Idea is to redesign a 3D printer</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
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<tr><td><p>Meeting and making a team commitment</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
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<tr><td><p>Brainstorm the possible ideas, discussing the found approaches.
 +
Need of more research</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
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<tr><td><p>Deciding the components and the programming language. Details still open.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>Made decision about the chassis, an Ultimaker2 clone. Pumping system will be a syringe pump from hackaday.io
 +
Two will look for the needed materials for the chassis, one the materials for the syringe pump and one will think about the detection system with an optic.</p>
 +
</td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
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<tr><td><p>We start to design the required CADs and learn the CAD software to do so. One of us is still doing some research about the optical detection system and how to do it.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>CADs are ready and the bill of materials is finished with the supplier information. We order the parts and talk to our workshop if they can make the needed adjustments.
 +
Our optics is chosen to be a Raspberry Pi NoIR Camera and we are using the OpenCV package to do the detection work.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>The ordered materials arrived and we started assembling the chassis. We talked to the workshop and they prepare some of the parts for us.
 +
One of our team members is preparing the OpenCV software and getting familiar with the camera</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>We have some problems assembling the chassis. Some parts need to be adapted. We like to have as much active measuring area as possible.
 +
The first 3D printed parts for the syringe pump are ready and we need to redesign some parts.
 +
The optics require IR light from underneath. Next week we are discussing the details of the construction</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>We will built a lightbox wit IR LEDs. To assure a homogeneous IR light we will use a diffuser plate illuminated from the sides.  One team member prepares the technical drawing.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>We are working on the chassis, the syringe pump and the optics.
 +
We have first object detections from the camera and the main parts of the chassis are assembled. </p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<td><p>Hier könnte Ihr labjournal stehen!</p>
 
<td><p>Hier könnte Ihr labjournal stehen!</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>We are waiting for the workshop to finish parts for the lightbox and the z-axis.
 +
We are working on the detection of rectangles and getting familiar with the Marlin software</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>We are playing with the stepper motors and waiting for some final parts for the x-y-axis.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>x-y-axis is mounted but we still need to adjust everything so it is moving smoothly
 +
The z-axis need a holdering made out of aluminum blocks we have to order. In that time we working on the GUI and the Qt software</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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The LVA&#8209;tag was deleted by PCR with the primer Del&#95;LVA&#95;fw and Del&#95;LVA&#95;rev.</p>
 
The LVA&#8209;tag was deleted by PCR with the primer Del&#95;LVA&#95;fw and Del&#95;LVA&#95;rev.</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>Mounted the z-axis and tuned the x-y axis. The optical software is nearly ready to use and we like to test it next week.
 +
We started connecting the electronics to an ATX power supply.
 +
The Raspberry Pi is set up and ready to use. We still have some problems with the wireless connection.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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Both fragments were ligated using the T4&#8209;Ligase by standard protocol.</p>
 
Both fragments were ligated using the T4&#8209;Ligase by standard protocol.</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>We installed the LEDs, relays and drivers for the LEDs. Sometimes they are not working properly but it seems to be a problem of the software, one is focusing on that task.
 +
X-y-Axis and z-axis movement works. We are installing the endstops to assure the steppers motors are stopping at the end of the movement. </p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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<b>BBa&#95;K1976001:</b><br>The ligation product was transformed into <i>E.&nbsp;coli</i> Top&nbsp;10 via heatshock. </p>
 
<b>BBa&#95;K1976001:</b><br>The ligation product was transformed into <i>E.&nbsp;coli</i> Top&nbsp;10 via heatshock. </p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>The GUI is on principle ready to use. Our RAMPS board is maybe broken and we buy another one. The syringe pump was tested and it works like expected. </p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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To check whether BBa&#95;I11020 was expressed properly a SDS&#8209;Page was made.</p>
 
To check whether BBa&#95;I11020 was expressed properly a SDS&#8209;Page was made.</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>Munich sent us their syringe pump. We are now printing the additional parts and like to test it.
 +
Still working on the electronics and one LED strip needs to be soldered again.
 +
We installed our lightbox and we can clearly see the objects and have target vectors which we are using for our auto tracking later</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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The LVA&#8209;tag was added by PCR with the overhang primer LVA&#95;GFP&#95;fw and LVA&#95;GFP&#95;rev.</p>
 
The LVA&#8209;tag was added by PCR with the overhang primer LVA&#95;GFP&#95;fw and LVA&#95;GFP&#95;rev.</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>We assembled the Munich syringe pump and tested it. It works good and we have new ideas for our system.
 +
We gave them feedback for their design.
 +
Our robot is working but we need to fix some bugs. Also we have to adjust some parameters.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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To check whether the genomic integration was successful a cPCR with attb&#95;fw and VR primers was made.</p>
 
To check whether the genomic integration was successful a cPCR with attb&#95;fw and VR primers was made.</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>We are testing the functionality of the robot. Unfortunately we still have no mVenus to work with, however we can do some test with our light detection.
 +
We encapsulated the robot and can control it via network.
 +
We are finalizing the robot</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
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</p>
 
</p>
 
</td></tr>
 
</td></tr>
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
+
<tr><td><p>Time is running and we are working on the last details. We are exited for the Jamboree.</p></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/7c/T--TU_Darmstadt--iconTech.png"/></td>
 
<td>robotics</td></tr>
 
<td>robotics</td></tr>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>
 
<tr><td></td><td><img class="labjournalIcon" src="https://static.igem.org/mediawiki/2016/7/78/T--TU_Darmstadt--iconMod.png"/></td>

Revision as of 18:53, 19 October 2016

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iGEM TU Darmstadt 2016

NOTEBOOK

Labjournal 2016

For half a year we were working in the wet lab    , developed a pipetting robot   and simulated the molecular dynamics of our proteins    . As well we would like to highlight certain social events   which helped us to further improve our team spirit.

Lab Week 1:
April 18 - April 24

Hier könnte Ihr labjournal stehen!

Meeting with the people interested in the technical part of the project, discussing the task and the possible ideas.

robotics
modeling
social
Lab Week 2:
April 25 - Mai 1

Hier könnte Ihr labjournal stehen!

Decision who will participate in the project and which direction we chose. 4 people forming the team and we do some research. Idea is to redesign a 3D printer

robotics
modeling
social
Lab Week 3:
May 2 - May 8

Hier könnte Ihr labjournal stehen!

Meeting and making a team commitment

robotics
modeling
social
Lab Week 4:
May 9 - May 15

Hier könnte Ihr labjournal stehen!

Brainstorm the possible ideas, discussing the found approaches. Need of more research

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Lab Week 5:
May 16 - May 22

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Deciding the components and the programming language. Details still open.

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Lab Week 6:
May 23 - May 29

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Made decision about the chassis, an Ultimaker2 clone. Pumping system will be a syringe pump from hackaday.io Two will look for the needed materials for the chassis, one the materials for the syringe pump and one will think about the detection system with an optic.

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Lab Week 7:
May 30 - June 5

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Lab Week 8:
June 6 - June 12

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We start to design the required CADs and learn the CAD software to do so. One of us is still doing some research about the optical detection system and how to do it.

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Lab Week 9:
June 13 - June 19

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Lab Week 10:
June 20 - June 26

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CADs are ready and the bill of materials is finished with the supplier information. We order the parts and talk to our workshop if they can make the needed adjustments. Our optics is chosen to be a Raspberry Pi NoIR Camera and we are using the OpenCV package to do the detection work.

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Lab Week 11:
June 27 - July 3

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The ordered materials arrived and we started assembling the chassis. We talked to the workshop and they prepare some of the parts for us. One of our team members is preparing the OpenCV software and getting familiar with the camera

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Lab Week 12:
July 4 - July 10

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We have some problems assembling the chassis. Some parts need to be adapted. We like to have as much active measuring area as possible. The first 3D printed parts for the syringe pump are ready and we need to redesign some parts. The optics require IR light from underneath. Next week we are discussing the details of the construction

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social
Lab Week 13:
July 11 - July 17

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We will built a lightbox wit IR LEDs. To assure a homogeneous IR light we will use a diffuser plate illuminated from the sides. One team member prepares the technical drawing.

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Lab Week 14:
July 18 - July 24

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We are working on the chassis, the syringe pump and the optics. We have first object detections from the camera and the main parts of the chassis are assembled.

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Lab Week 15:
July 25 - July 31

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Lab Week 16:
August 1 - August 7

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We are waiting for the workshop to finish parts for the lightbox and the z-axis. We are working on the detection of rectangles and getting familiar with the Marlin software

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Lab Week 17:
August 8 - August 14

Reporter System
BBa_K1976002:
The mVenus-LVA-gene was synthesized by idt and amplified through PCR using the SP-amplify-fw-Rep1 (fw) and SP-amplify-rev-Rep2 (REV) oligonucleotides.

We are playing with the stepper motors and waiting for some final parts for the x-y-axis.

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Lab Week 18:
August 15 - August 21

Reporter System
BBa_K1976002:
The PCR product was verified by agarose gel electrophoresis and subsequently purified. The mVenus amplicon was cut with EcoRI and PstI and ligated into a pSB1C3 vector using T4-ligase.

x-y-axis is mounted but we still need to adjust everything so it is moving smoothly The z-axis need a holdering made out of aluminum blocks we have to order. In that time we working on the GUI and the Qt software

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Lab Week 19:
August 22 - August 28

Reporter System
BBa_K1976002:
The ligation product was transformed into E.coli Top 10 by heat shock transformation and the cells were spread out on a CMP agar plate.

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Lab Week 20:
August 29 - September 4

Reporter System
BBa_K1976002:
Clones were screened with colony PCR using VF2 and VR oligonucleotides. Plasmid DNA was isolated from positive clones.
Metabolic burden
BBa_K1976001:
The BioBrick BBa_I11020 was synthezised with a LVA‑tag and with B0034 as a ribosome binding site by IDT.

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Lab Week 21:
September 5 - September 11

Reporter System
BBa_K1976002:
Isolated Plasmid DNA Sequence was verified by Sanger Sequencing.
Metabolic burden
BBa_K1976001:
The LVA‑tag was deleted by PCR with the primer Del_LVA_fw and Del_LVA_rev.

Mounted the z-axis and tuned the x-y axis. The optical software is nearly ready to use and we like to test it next week. We started connecting the electronics to an ATX power supply. The Raspberry Pi is set up and ready to use. We still have some problems with the wireless connection.

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Lab Week 22:
September 12 - September 18

Reporter System
BBa_K1976003:
The mVenus-LVA-pSB1C3 plasmid was cut with EcoRI and PstI and ligated into a T7-RBS-pSB1A3 plasmid which was cut with SpeI and PstI.
Metabolic burden
BBa_K1976000:
The BioBrick BBa_E0240 was cut with the restriction enzymes XbaI and PstI.
BBa_J61002 was cut with SpeI and PstI.
The two restriction products were ligated by standard T4‑Ligation protocol.
BBa_K1976001:
B0034‑BBa_I11020 was cut with XbaI and PstI.
BBa_J23101 was cut with SpeI and PstI.
Both fragments were ligated using the T4‑Ligase by standard protocol.

We installed the LEDs, relays and drivers for the LEDs. Sometimes they are not working properly but it seems to be a problem of the software, one is focusing on that task. X-y-Axis and z-axis movement works. We are installing the endstops to assure the steppers motors are stopping at the end of the movement.

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social
Lab Week 23:
September 19 - September 25

Reporter System
BBa_K1976003:
The ligation product was transformed into E.coli Top 10 by heat shock transformation and the cells were spread out on a AMP agar plate.
Metabolic burden
BBa_K1976000:
After plasmid preparation, the plasmid was cut with XbaI and PstI.
We had the attp‑site BBa_11023 flanked by the two additional bidirectional transcription terminators BBa_1001 synthesized by IDT. The attp‑site of BBa_11023 was not the original λ‑attp‑site. Instead the sequence corresponded to the attP2‑site used in the Gateway ® cloning system.
The construct was cut with EcoRI and SpeI. The pSB1C3 backbone was cut with EcoRI and PstI. Together with the previously cut BBa_J61002‑BBa_E0240 construct the synthesized BBa_1001‑BBa_11023‑BBa_1001 construct was ligated in the cut pSB1C3.
BBa_K1976001:
The ligation product was transformed into E. coli Top 10 via heatshock.

The GUI is on principle ready to use. Our RAMPS board is maybe broken and we buy another one. The syringe pump was tested and it works like expected.

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social
Lab Week 24:
September 26 - October 2

Reporter System
BBa_K1976003:
Clones were screened with colony PCR using VF2 and VR oligonucleotides. Plasmid DNA was isolated from positive clones .
Metabolic burden
BBa_K1976000:
The attP2‑site was mutated to λ‑attp via Quick‑Change‑PCR, using the following primers: QC_attp_fw and QC_attp_rev (for more details see our primerlist).
BBa_K1976001:
To check whether BBa_I11020 was expressed properly a SDS‑Page was made.

Munich sent us their syringe pump. We are now printing the additional parts and like to test it. Still working on the electronics and one LED strip needs to be soldered again. We installed our lightbox and we can clearly see the objects and have target vectors which we are using for our auto tracking later

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modeling
social
Lab Week 25:
October 3 - October 9

Reporter System
BBa_K1976003:
The isolated Plasmid DNA was verified by Sanger sequencing.
Metabolic burden
BBa_K1976000:
The LVA‑tag was added by PCR with the overhang primer LVA_GFP_fw and LVA_GFP_rev.

We assembled the Munich syringe pump and tested it. It works good and we have new ideas for our system. We gave them feedback for their design. Our robot is working but we need to fix some bugs. Also we have to adjust some parameters.

robotics
modeling
social
Lab Week 26:
October 10 - October 16

Reporter System
BBa_K1976003:
Subsequently the psB1A3 vector containing brick was cut with EcoRI and PstI and ligated into a pSB1C3 vector using T4‑Ligase.
Metabolic burden
BBa_K1976000:
For plasmid curing the final plasmid construct and the low copy backbone pSB4A5 were cut with EcoRI and PstI and then ligated.
BBa_K1976001:
For plasmid curing the final plasmid construct and the low copy vector pSB4K5 were cut with EcoRI and PstI and then ligated.
BBa_K1976000 and BBa_K1976001 were cotransformed into E. coli Top 10.
To check whether the genomic integration was successful a cPCR with attb_fw and VR primers was made.

We are testing the functionality of the robot. Unfortunately we still have no mVenus to work with, however we can do some test with our light detection. We encapsulated the robot and can control it via network. We are finalizing the robot

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social
Lab Week 27:
October 17 - October 23

Metabolic burden
Plasmid curing could not be performed due to lack of time.

Time is running and we are working on the last details. We are exited for the Jamboree.

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modeling
social