Difference between revisions of "Team:TAS Taipei/Composite Part"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">Composite Part special prize</a>. </p>
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<a href="https://2016.igem.org/Team:TAS_Taipei/Background"><h4 class="dropdown-toggle disabled" data-toggle="dropdown"><b>PROJECT</b></h4></a>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Background">Background</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Achievements">Achievements</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Collaborations">Collaborations</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Parts">Parts</a></h5>
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<img src="https://static.igem.org/mediawiki/2016/1/11/T--TAS_Taipei--TAS_Icon_Project.png">
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<h4><b>Cataracts</b> - the leading cause of blindness. Find out how we can non-invasively <b>treat</b> and <b>prevent</b> cataract formation.</b></h4>
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<a href="https://2016.igem.org/Team:TAS_Taipei/Experimental_Summary"><h4 class='dropdown-toggle disabled' data-toggle="dropdown"><b>EXPERIMENTAL</b></h4></a>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Experimental_Summary#lensmodel">Lens Cataract Model</a></h5>
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</div>
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<div>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Experimental_Summary#construct">Prevention and Treatment Constructs </a></h5>
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<div>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Experimental_Summary#prototype">Delivery Prototype</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Notebook">Notebook</a></h5>
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<div style='height:140px;'>
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<img src="https://static.igem.org/mediawiki/2016/b/b0/T--TAS_Taipei--TAS_Icon_Experiments.png">
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                        <h4>We build <b>constructs</b> to make our great ideas become reality. Follow along our discovery of exciting science!</h4>
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<a href="https://2016.igem.org/Team:TAS_Taipei/Model"><h4 class='dropdown-toggle disabled' data-toggle="dropdown"><b>MODEL</b></h4></a>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Model#GSRFunc">GSR Function</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Model#prototype">Prototype Delivery</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Model#treatment">CH25H Treatment</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Model#software">Calculator</a></h5>
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<div style='height:140px;'>
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<img src="https://static.igem.org/mediawiki/2016/c/ca/T--TAS_Taipei--TAS_Icon_Model.png">
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<h4><b>Computational Biology</b> provides us models that we cannot easily test experimentally. Click to find out the results of our modeling, and the math behind it!</h4>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Human_Practices#research">Research</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Human_Practices#outreach">Outreach</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Human_Practices#impact">Impact</a></h5>
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<div style='height:140px;'>
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<img src="https://static.igem.org/mediawiki/2016/b/b9/T--TAS_Taipei--TAS_Icon_HP.png">
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<h4>We don't just grow cool bacteria. We make a <b>difference</b>. Find out how we tackled <b>social aspects</b> of this project.</h4>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Safety#overview">Overview</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Safety#biosafety">Biosafety</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Safety#riskreduction">Risk Reduction Methods</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Safety#furtherquestion">Further Questions</a></h5>
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</div>
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<img src="https://static.igem.org/mediawiki/2016/7/7e/T--TAS_Taipei--TAS_Icon_Safety.png">
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<h4><b>Safety first.</b> Especially in a lab. Here's how we maintained and investigated the integrity and security of our work environment.</h4>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Team#members">Members</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Team#about">About</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Attributions">Attributions</a></h5>
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Wiki_Standard_Pages">Wiki Standard Pages</a></h5>
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<img src="https://static.igem.org/mediawiki/2016/c/c6/T--TAS_Taipei--TAS_Icon_Team.png">
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<h4>Every iGEM project is the accumulation of an entire year's hard work by a group of cheerful students. <b>Meet the team!</b></h4>
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<a href="https://2016.igem.org/Team:TAS_Taipei" style='text-decoration: none'><img src="https://static.igem.org/mediawiki/2016/c/c6/T--TAS_Taipei--TAS_iGEM_Logo.png
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<h2 style="font-family:'Lato';letter-spacing:10px;color: white; font-size: 60px;  margin-top: 0;  margin-bottom: 0;"><b>
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C&#9678;UNTERACTS</b></h2>
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</a>
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<a href="https://igem.org/HS"><img src="https://static.igem.org/mediawiki/2016/6/6e/T--TAS_Taipei--TAS_Icon_Logo2.png" alt="" style="width: 100px;"></a>
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                        <li><a href="#submit">Table of Parts</a></li>
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                        <li><a href="#diagram">Parts Diagrams</a></li>
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<h1>Composite Part</h1>
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                                  <p> The composite parts consist of intermediates, a 10x histidine Tag and a terminator, allowing users to purify the protein. In addition, a composite part that puts green fluorescent protein behind the strong promoter, RBS, and in front of the histidine tag can be used to test protein expression levels and protein purification.</p>
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                                        <h2 id="submit">Table of Parts</h2>
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                                                    <th>Part #</th>
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                                                    <th>Name</th>
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                                                    <th>Type</th>
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                                                    <th>Link</th>
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                                                    <td>BBa_K1898150</td>
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                                                    <td>Strong Promoter + Strong RBS + CH25H + 10x his-tag + Double Terminator</td>
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                                                    <td>Composite</td>
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                                                    <td><a href="http://parts.igem.org/Part:BBa_K1898150">Link</a></td>
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                                                    <td>BBa_K1898250</td>
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                                                    <td>Strong Promoter + Strong RBS + GSR + 10x his-tag + Double Terminator</td>
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                                                    <td>Composite</td>
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                                                    <td><a href="http://parts.igem.org/Part:BBa_K1898250">Link</a></td>
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                                                    <td>BBa_K1898450</td>
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                                                    <td>Strong Promoter + Strong RBS + CRYAB + 10x his-tag + Double Terminator</td>
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                                                    <td>Composite</td>
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                                                    <td><a href="http://parts.igem.org/Part:BBa_K1898450">Link</a></td>
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                                                </tr>
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                                                <tr>
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                                                    <td>BBa_K1898550</td>
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                                                    <td>Strong Promoter + Strong RBS + GFP + 10x his-tag + Double Terminator</td>
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                                                    <td>Composite</td>
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                                                    <td><a href="http://parts.igem.org/Part:BBa_K1898550">Link</a></td>
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                                                </tr>
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                                    <h2 id="diagram">Parts Diagrams</h2>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
 
  
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<div class="backgroundpic">
 +
<img src="https://static.igem.org/mediawiki/2016/e/e5/T--TAS_Taipei--Taipei_Background_1.0.jpg" style="z-index:0" width="100%" height="100%" id="bg" alt="" >
 
</div>
 
</div>
  
 +
<script>
 +
var nowRadius = 0
 +
$(function() {
 +
    if (screen.width > 1800)
 +
      $("#slideoutco").fadeOut(0);
 +
   
 +
    $({blurRadius: 0}).animate({blurRadius: 10}, {
 +
        duration: 20000,
 +
        easing:'swing', // or "linear"
 +
                        // use jQuery UI or Easing plugin for more options
 +
        step: function() {
 +
            console.log(this.blurRadius);
 +
            if ($("#bluebutton").hasClass("isOn") ) {return;};
 +
            if($('#redbutton').hasClass("isOn") ) {return;}
 +
            $('.backgroundpic').css({
 +
                "-webkit-filter": "blur("+this.blurRadius+"px)",
 +
                "filter": "blur("+this.blurRadius+"px)"
 +
 +
            });
 +
            nowRadius = this.blurRadius;
 +
        var LOCSnum = Math.round(nowRadius*6/9);
 +
        var NineLOCSnum = Math.round(nowRadius*6/9*10);
 +
        /**if (NineLOCSnum > 20) switchMessage("YELLOW","<b> Oh no! </b> Cataracts are forming! Click on the Eyedrop Tab and get <b>TREATMENT</b> eyedrops!", currentColor()=="GREEN");
 +
        if (NineLOCSnum == 55) $("#slideoutco").fadeOut(1000);
 +
        if (NineLOCSnum > 60) switchMessage("RED","Your cataracts are very severe. You need to get TREATMENT fast by clicking the Eyedrop Tab", currentColor()=="YELLOW");*/
 +
        if (LOCSnum > 6) {LOCSnum = "6+"; $('#LOCS').text(LOCSnum+"");}
 +
        else $('#LOCS').text(LOCSnum+"");
 +
       
 +
        if (LOCSnum ==0) document.getElementById('bluebutton').click();
 +
        }
 +
    });
 +
 +
});
 +
 +
startBlur= function(speed) {
 +
    $({blurRadius: nowRadius}).animate({blurRadius: 10}, {
 +
        duration: speed,
 +
        easing: 'swing', // or "linear"
 +
                        // use jQuery UI or Easing plugin for more options
 +
        step: function() {
 +
            console.log(this.blurRadius);
 +
            if ($("#bluebutton").hasClass("isOn") ) {return;};
 +
            if($('#redbutton').hasClass("isOn") ) {return;}
 +
 +
            $('.backgroundpic').css({
 +
                "-webkit-filter": "blur("+this.blurRadius+"px)",
 +
                "filter": "blur("+this.blurRadius+"px)"
 +
 +
            });
 +
 +
        nowRadius = this.blurRadius;
 +
        var LOCSnum = Math.round(nowRadius*6/9);
 +
        var NineLOCSnum = Math.round(nowRadius*6/9*10);
 +
        /**if (NineLOCSnum == 15) $("#slideoutco").fadeOut(400);
 +
        if (NineLOCSnum > 20) switchMessage("RED","<b>Cataracts</b> are creeping back again! Click the PREVENTION eyedrop to add GSR into your eyes!", currentColor()=="BLUE");*/
 +
        if (LOCSnum > 6) {LOCSnum = "6+"; $('#LOCS').text(LOCSnum+"");}
 +
        else $('#LOCS').text(LOCSnum+" ");
 +
       
 +
       
 +
        }
 +
    });
 +
};
 +
 +
stopBlur= function(speed) {
 +
 +
    $({blurRadius: nowRadius}).animate({blurRadius: 0}, {
 +
        duration: speed,
 +
        easing: 'swing', // or "linear"
 +
                        // use jQuery UI or Easing plugin for more options
 +
        step: function() {
 +
            console.log(this.blurRadius);
 +
        if ($("#redbutton").hasClass("isOn") ) {} else{return;};
 +
            $('.backgroundpic').css({
 +
                "-webkit-filter": "blur("+this.blurRadius+"px)",
 +
                "filter": "blur("+this.blurRadius+"px)"
 +
 +
            });
 +
        nowRadius = this.blurRadius;
 +
        var LOCSnum = Math.round(nowRadius*6/9);
 +
        var NineLOCSnum = Math.round(nowRadius*6/9*10);
 +
         
 +
        /**if (NineLOCSnum < 68){ if (NineLOCSnum > 10) if(currentColor()=="RED" ||  currentColor() == "BLUE") $("#slideoutco").fadeOut(0); switchMessage("YELLOW","<b> Treating</b> your cataract with <b>25HC Eyedrops </b>", currentColor()=="RED");
 +
                              }
 +
        if (NineLOCSnum == 9) $("#slideoutco").fadeOut(0);
 +
       
 +
           
 +
        if (NineLOCSnum < 5) {
 +
            if ($("#bluebutton").hasClass("isOn")){
 +
                   
 +
                switchMessage("GREEN","<b>Your eyes are <i>pernamently</i> crystal clear!</b> Treatment is not needed, so don't forget to turn it off!. Click the question mark to learn more.", currentColor()=="BLUE")
 +
            }
 +
            else switchMessage("BLUE","Your eyes are crystal clear! To avoid waste, please turn off the <b>TREATMENT</b> eyedrop.", currentColor()=="YELLOW");
 +
        }*/
 +
        if (LOCSnum > 6) LOCSnum = "6+";
 +
        else LOCSnum = LOCSnum +" ";
 +
        $('#LOCS').text(LOCSnum+"");   
 +
       
 +
        }
 +
    });
 +
   
 +
   
 +
};
 +
 +
function chooseBlur() {
 +
  if ($("#redbutton").hasClass("isOn") )
 +
      {
 +
      stopBlur(4500);
 +
 
 +
            }
 +
 
 +
    else {
 +
            if($("#bluebutton").hasClass("isOn") ) {}
 +
            else {startBlur(12000);}
 +
          }
 +
    /**if (!$("#redbutton").hasClass("isOn"))
 +
        if ($("#bluebutton").hasClass("isOn"))
 +
            {
 +
                $("#slideoutco").fadeOut(0);
 +
                switchMessage("BLUE","Great! Furthur cataract formation is prevented with GSR Eyedrops. Now use <b>TREATMENT</b> eyedrops one last time.", currentColor()=="RED")
 +
            }*/
 +
};
 +
 +
 +
</script>
 +
 +
<script>
 +
 +
function switchToggleB() {
 +
    if ( $("#bluebutton").hasClass("isOn") ) {
 +
        $("#bluebutton").removeClass("isOn"); }
 +
  else { $("#bluebutton").addClass("isOn"); }
 +
};
 +
function switchToggleR() {
 +
    if ( $("#redbutton").hasClass("isOn") ) {
 +
        $("#redbutton").removeClass("isOn"); }
 +
  else { $("#redbutton").addClass("isOn"); }
 +
};
 +
   
 +
function currentColor() {
 +
    if ($("#slidecontenttext").hasClass("alert-danger"))
 +
        return "RED";
 +
    if ($("#slidecontenttext").hasClass("alert-warning"))
 +
        return "YELLOW";
 +
    if ($("#slidecontenttext").hasClass("alert-info"))
 +
        return "BLUE";
 +
    if ($("#slidecontenttext").hasClass("alert-success"))
 +
        return "GREEN";
 +
    else return "NONE"
 +
};
 +
function switchMessage(color, textInside, refresh) {
 +
    var entirebox = document.getElementById("slideoutco");
 +
 +
    if (refresh)
 +
    {$(entirebox).fadeOut(100);
 +
    if ($("#slidecontenttext").hasClass("alert-danger"))
 +
        $("#slidecontenttext").removeClass("alert-danger");
 +
    if ($("#slidecontenttext").hasClass("alert-warning"))
 +
        $("#slidecontenttext").removeClass("alert-warning");
 +
    if ($("#slidecontenttext").hasClass("alert-info"))
 +
        $("#slidecontenttext").removeClass("alert-info");
 +
    if ($("#slidecontenttext").hasClass("alert-success"))
 +
        $("#slidecontenttext").removeClass("alert-success");
 +
    if (color == "RED")
 +
        $("#slidecontenttext").addClass("alert-danger");
 +
    if (color == "YELLOW")
 +
        $("#slidecontenttext").addClass("alert-warning");
 +
    if (color == "BLUE")
 +
        $("#slidecontenttext").addClass("alert-info");
 +
    if (color == "GREEN")
 +
        $("#slidecontenttext").addClass("alert-success");
 +
   
 +
   
 +
    $("#slidecontenttext").html(textInside);
 +
    $(entirebox).fadeIn(500);
 +
    }
 +
   
 +
};
 +
 +
 +
</script>
 +
 +
 +
 +
 +
 +
 +
<canvas id="canvas-container" style = "z-index:-1" hidden></canvas>
 +
<script type="text/javascript" src='https://2015.igem.org/Template:TAS_Taipei/js/field?action=raw&ctype=text/javascript' hidden></script/>
 +
 +
 +
 +
<script type="text/javascript">
 +
/* Toggle between adding and removing the "active" and "show" classes when the user clicks on one of the "Section" buttons. The "active" class is used to add a background color to the current button when its belonging panel is open. The "show" class is used to open the specific accordion panel */
 +
var acc = document.getElementsByClassName("accordion");
 +
var i;
 +
 +
for (i = 0; i < acc.length; i++) {
 +
    acc[i].onclick = function(){
 +
        this.classList.toggle("active");
 +
        this.nextElementSibling.classList.toggle("show");
 +
    }
 +
}
 +
</script>
 +
 +
<script type="text/javascript">
 +
$("#category_navbar a").on('click', function(event) {
 +
    // Make sure this.hash has a value before overriding default behavior
 +
    if (this.hash !== "") {
 +
      // Prevent default anchor click behavior
 +
      event.preventDefault();
 +
 +
      // Store hash0
 +
      var hash = this.hash;
 +
 +
      // Using jQuery's animate() method to add smooth page scroll
 +
      // The optional number (800) specifies the number of milliseconds it takes to scroll to the specified area
 +
      $('html, body').animate({
 +
        scrollTop: $(hash).offset().top
 +
      }, 800, function(){
 +
 
 +
        // Add hash (#) to URL when done scrolling (default click behavior)
 +
        window.location.hash = hash;
 +
      });
 +
    }  // End if
 +
  });
 +
 +
 +
</script>
 +
 +
<div id="slideout">
 +
    <div id="slidecontent">
 +
        <h3>Prevention</h3>
 +
        <h5>GSR Eyedrop</h5>
 +
        <label class="switch">
 +
            <input id="bluebutton" onClick="switchToggleB(); chooseBlur()" type="checkbox">
 +
            <div class="slider round bluecolorbutton"></div>
 +
        </label>
 +
<br>
 +
 +
        <h3>Treatment</h3>
 +
        <h5>25HC Eyedrop</h5>
 +
        <label class="switch">
 +
            <input id="redbutton" onClick="switchToggleR(); chooseBlur()" type="checkbox">
 +
            <div class="slider round redcolorbutton"></div>
 +
        </label>
 +
        <h4><b> LOCS: <span id="LOCS">0 &#160;</span></b> &#160; &#160; <a href="https://2016.igem.org/Team:TAS_Taipei/Wiki_Standard_Pages#Animation"><button  type="button" class="btn btn-danger btn-md">?</button> </a>
 +
 +
    </div>
 +
    <div id="clickme">
 +
    <h2 class="vertical-text" style="Lato" !important>
 +
            <br> Eyedrops
 +
    </h2>
 +
        <br><br><br>
 +
       
 +
    </div>
 
</div>
 
</div>
 +
 +
<div id="slideoutco">
 +
    <div id="slidecontentco">
 +
        <div id="slidecontenttext" class="alert alert-danger">
 +
            <p style="font-size:14px !important"> <a href="#" class="close" data-dismiss="alert">&times;</a>
 +
                <strong>Zoom out to see animation.</strong> &#160; &#160; &#160; &#160; &#160;<a href="https://2016.igem.org/Team:TAS_Taipei/Wiki_Standard_Pages#Animation"><button  type="button" class="btn btn-danger btn-sm">?</button> </a><span style="font-size:14px"><br>Your screen resolution is too low unless you zoom out </span></p>
 +
           
 +
        </div>
 +
    </div>
 +
</div>
 +
 +
<javascript>
 +
   
 +
</javascript>
 +
 +
<style type='text/css'>
 +
 +
#slideout {
 +
    background: #FFD700;
 +
    position: fixed;
 +
    height: 300px;
 +
    width: 200px;
 +
    top: 30%;
 +
    right:-150px;
 +
    padding-left: 60px;
 +
    z-index:30;
 +
    border-radius: 25px;
 +
 +
 +
}
 +
   
 +
#slideoutco {
 +
    position: fixed;
 +
    height: 200px;
 +
    width: 300px;
 +
    top: 78%;
 +
    right:1%;
 +
    padding-left: 10px;
 +
    padding-right: 10px;
 +
    border-radius: 10px;
 +
    z-index:30;
 +
}
 +
   
 +
#clickme {
 +
    position: absolute;
 +
    top: 0; left: 0;
 +
    height: 300px;
 +
    width: 50px;
 +
    background: #FFBA49;
 +
    z-index:30;
 +
    border-radius: 25px 5px 5px 25px;
 +
}
 +
 +
#slidecontent {
 +
    float:left;
 +
}
 +
   
 +
#slidecontentco {
 +
    float:left;
 +
}
 +
 +
.vertical-text {
 +
transform: rotate(90deg);
 +
transform-origin: left bottom 0;
 +
  float: left;
 +
}
 +
/* The switch - the box around the slider */
 +
.switch {
 +
  position: relative;
 +
  display: inline-block;
 +
  width: 60px;
 +
  height: 34px;
 +
}
 +
 +
/* Hide default HTML checkbox */
 +
.switch input {display:none;}
 +
 +
/* The slider */
 +
.slider {
 +
  position: absolute;
 +
  cursor: pointer;
 +
  top: 0;
 +
  left: 0;
 +
  right: 0;
 +
  bottom: 0;
 +
  background-color: #ccc;
 +
  -webkit-transition: .4s;
 +
  transition: .4s;
 +
}
 +
 +
 +
.slider:before {
 +
  position: absolute;
 +
  content: "";
 +
  height: 26px;
 +
  width: 26px;
 +
  left: 4px;
 +
  bottom: 4px;
 +
  background-color: white;
 +
  -webkit-transition: .4s;
 +
  transition: .4s;
 +
}
 +
 +
input:checked + .slider.bluecolorbutton {
 +
  background-color: #2196F3;
 +
}
 +
 +
input:focus + .slider.bluecolorbutton {
 +
  box-shadow: 0 0 1px #2196F3;
 +
}
 +
 +
input:checked + .slider.redcolorbutton {
 +
  background-color: #FF0000;
 +
}
 +
 +
input:focus + .slider.redcolorbutton {
 +
  box-shadow: 0 0 1px #FF0000;
 +
}
 +
 +
 +
 +
input:checked + .slider:before {
 +
  -webkit-transform: translateX(26px);
 +
  -ms-transform: translateX(26px);
 +
  transform: translateX(26px);
 +
}
 +
 +
/* Rounded sliders */
 +
.slider.round {
 +
  border-radius: 34px;
 +
}
 +
 +
.slider.round:before {
 +
  border-radius: 50%;
 +
}
 +
input[type=text] {
 +
    width: 100%;
 +
    padding: 12px 20px;
 +
    margin: 8px 0;
 +
    box-sizing: border-box;
 +
    border: 3px solid #ccc;
 +
    -webkit-transition: 0.5s;
 +
    transition: 0.5s;
 +
    outline: none;
 +
}
 +
input[type=text]:focus {
 +
    border: 3px solid #555;
 +
}
 +
</style>
 +
<script>
 +
$(function () {
 +
    var rightVal = -150;
 +
   
 +
        $("#clickme").click(function () {
 +
       
 +
        rightVal = (rightVal * -1) - 150;
 +
        $(this).parent().animate({right: rightVal + 'px'}, {queue: false, duration: 500});
 +
    });
 +
   
 +
});
 +
 +
 +
 +
</script>
 +
 +
 +
</body>
  
 
</html>
 
</html>

Latest revision as of 15:52, 19 October 2016

Composite Part - TAS Taipei iGEM Wiki




C◎UNTERACTS

Composite Part

The composite parts consist of intermediates, a 10x histidine Tag and a terminator, allowing users to purify the protein. In addition, a composite part that puts green fluorescent protein behind the strong promoter, RBS, and in front of the histidine tag can be used to test protein expression levels and protein purification.

Table of Parts

Part # Name Type Link
BBa_K1898150 Strong Promoter + Strong RBS + CH25H + 10x his-tag + Double Terminator Composite Link
BBa_K1898250 Strong Promoter + Strong RBS + GSR + 10x his-tag + Double Terminator Composite Link
BBa_K1898450 Strong Promoter + Strong RBS + CRYAB + 10x his-tag + Double Terminator Composite Link
BBa_K1898550 Strong Promoter + Strong RBS + GFP + 10x his-tag + Double Terminator Composite Link

Parts Diagrams

>




Prevention

GSR Eyedrop

Treatment

25HC Eyedrop

LOCS: 0      


Eyedrops




× Zoom out to see animation.          
Your screen resolution is too low unless you zoom out