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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes"> design special prize</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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By talking about your design work on this page, there is one medal criterion that you can attempt to meet, and one award that you can apply for. If your team is going for a gold medal by building a functional prototype, you should tell us what you did on this page.
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<p>This is a prize for the team that has developed a synthetic biology product to solve a real world problem in the most elegant way. The students will have considered how well the product addresses the problem versus other potential solutions, how the product integrates or disrupts other products and processes, and how its lifecycle can more broadly impact our lives and environments in positive and negative ways.</p>
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If you are working on art and design as your main project, please join the art and design track. If you are integrating art and design into the core of your main project, please apply for the award by completing this page.
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<p>Teams who want to focus on art and design should be in the art and design special track. If you want to have a sub-project in this area, you should compete for this award.</p>
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<p>Tuberculosis (TB) is one of the leading causes of death worldwide according the World Health Organization (source:http://www.who.int/gho/tb/en/), despite the fact that it is curable and treatments are often completely paid for by the government. Even so, 1.4 million died from TB in 2014 (source:http://www.who.int/mediacentre/factsheets/fs104/en/). Access to treatment isn't the problem. The problem is patients with TB are not being diagnosed until it is too late, since current methods are either cheap but only 50% sensitive, or are accurate but, prohibitively expensive or require access to advanced medical facilities.</p>
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                <p>Our team decided to tackle this problem by developing a genetic switch that could detect TB biomarkers with less hassle than ELIZA and other similar methods of protein detection. In the regions where TB is most prevalent, namely africa and sub-continental asia, access to labs and lab equipment is difficult. Our genetic switch is designed to be incorporated into a paper-based test strip that could be easily self administered for screening purposes with no specialized equipment. </p>
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                <p>Our switch design this year relies on two unique aptamers that both bind to the target protein. For the purpose of our experiments, this target was thrombin because it is readily available and has been thoroughly characterized. However, we anticipate converting the target protein to a TB biomarker such as CFP-10 (INCLUDE SOURCE FOR CFP-10) would not be very difficult. These two aptamers have been designed with sequences that enable the free-floating tails to ligate with each other through proximity dependent ligation. When the aptamers bind to the target protein and ligate together, they allow expression of the lacZ alpha fragment, which enables free floating lacZ to convert Xgal to __________ and cause a blue colorimetric output. </p>
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                <p>When this system is freeze dried onto the paper test strip it can last up to a year with no refrigeration (SOURCE). To use, a consumer need only rehydrate with a sputum sample and watch for the color to change.</p>
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Revision as of 14:39, 30 August 2016


Home Project Modeling Team BioBrick Human Practices Safety Awards

  • Description

    Tuberculosis (TB) is one of the leading causes of death worldwide according the World Health Organization (source:http://www.who.int/gho/tb/en/), despite the fact that it is curable and treatments are often completely paid for by the government. Even so, 1.4 million died from TB in 2014 (source:http://www.who.int/mediacentre/factsheets/fs104/en/). Access to treatment isn't the problem. The problem is patients with TB are not being diagnosed until it is too late, since current methods are either cheap but only 50% sensitive, or are accurate but, prohibitively expensive or require access to advanced medical facilities.

    Our team decided to tackle this problem by developing a genetic switch that could detect TB biomarkers with less hassle than ELIZA and other similar methods of protein detection. In the regions where TB is most prevalent, namely africa and sub-continental asia, access to labs and lab equipment is difficult. Our genetic switch is designed to be incorporated into a paper-based test strip that could be easily self administered for screening purposes with no specialized equipment.

    Our switch design this year relies on two unique aptamers that both bind to the target protein. For the purpose of our experiments, this target was thrombin because it is readily available and has been thoroughly characterized. However, we anticipate converting the target protein to a TB biomarker such as CFP-10 (INCLUDE SOURCE FOR CFP-10) would not be very difficult. These two aptamers have been designed with sequences that enable the free-floating tails to ligate with each other through proximity dependent ligation. When the aptamers bind to the target protein and ligate together, they allow expression of the lacZ alpha fragment, which enables free floating lacZ to convert Xgal to __________ and cause a blue colorimetric output.

    When this system is freeze dried onto the paper test strip it can last up to a year with no refrigeration (SOURCE). To use, a consumer need only rehydrate with a sputum sample and watch for the color to change.