Difference between revisions of "GDSYZX-United/NOTEBOOK/day note"
Thomasboboto (Talk | contribs) |
Thomasboboto (Talk | contribs) |
||
| Line 112: | Line 112: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.7.31</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.7.31</div></p> |
| − | <small class="text-navy"><div class="text_font">9:00</div></small> | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>Extract genome from Arabidopsis Thaliana</strong></div> | <div class="m-b-xs text_font"><strong>Extract genome from Arabidopsis Thaliana</strong></div> | ||
| − | <div class="text_font">The genome has been degraded greatly,possibly because we didn’t grind the leaves in very low temperature to inhibit the nuclease.We have to do it again.</div> | + | <p><div class="text_font">The genome has been degraded greatly,possibly because we didn’t grind the leaves in very low temperature to inhibit the nuclease.We have to do it again.</div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 124: | Line 124: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.7.31</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.7.31</div></p> |
| − | <small class="text-navy"><div class="text_font">13:30</div></small> | + | <small class="text-navy"><p><div class="text_font">13:30</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>Extract genome from Arabidopsis Thaliana</strong> | <div class="m-b-xs text_font"><strong>Extract genome from Arabidopsis Thaliana</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We extracted it the genome successfully.But the genome has still been degraded a little. (Since we don’t have liquid nitrogen in our lab,so we grinded the leaves in an ice box. )We will use the genome to do the following PCR. | We extracted it the genome successfully.But the genome has still been degraded a little. (Since we don’t have liquid nitrogen in our lab,so we grinded the leaves in an ice box. )We will use the genome to do the following PCR. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 139: | Line 139: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.1</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.1</div></p> |
| − | <small class="text-navy"><div class="text_font">9:00</div></small> | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We use PCR to generate and to amplify our target DNA fragments:promotercop1,promoter phyB,promoterpif1,gene hhl1,gene pHhl,gene flu.</strong> | <div class="m-b-xs text_font"><strong>We use PCR to generate and to amplify our target DNA fragments:promotercop1,promoter phyB,promoterpif1,gene hhl1,gene pHhl,gene flu.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We failed to get all our target DNA fragments .We will check the primers and the PCR program ,and then do it again. | We failed to get all our target DNA fragments .We will check the primers and the PCR program ,and then do it again. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 154: | Line 154: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.2</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.2</div></p> |
| − | <small class="text-navy"><div class="text_font">9:00</div></small> | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>Do the PCR again to get our target DNA fragments.</strong> | <div class="m-b-xs text_font"><strong>Do the PCR again to get our target DNA fragments.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We PCR gene pHhl1,gene hhl1,gene flu successfully. | We PCR gene pHhl1,gene hhl1,gene flu successfully. | ||
We will PCR cop1 together with pif1,phyB tomorrow. | We will PCR cop1 together with pif1,phyB tomorrow. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 170: | Line 170: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.3</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p> |
| − | <small class="text-navy"><div class="text_font">9:00</div></small> | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We use PCR to get cop1,phyB and pif1</strong> | <div class="m-b-xs text_font"><strong>We use PCR to get cop1,phyB and pif1</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
In the first PCR we get cop 1 and pif1,we get higher concentration of phyB in the second time PCR. | In the first PCR we get cop 1 and pif1,we get higher concentration of phyB in the second time PCR. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 185: | Line 185: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.3</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p> |
| − | <small class="text-navy"><div class="text_font">13:30</div></small> | + | <small class="text-navy"><p><div class="text_font">13:30</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We use a kit box to purify our PCR products,so we can get our genes.</strong> | <div class="m-b-xs text_font"><strong>We use a kit box to purify our PCR products,so we can get our genes.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 200: | Line 200: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.3</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p> |
| − | <small class="text-navy"><div class="text_font">in the afternoon</div></small> | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| Line 207: | Line 207: | ||
EcorⅠ.After this process ,the gene fragments will have one overhang at both ends.The overhangs will help them splice other fragments. </strong> | EcorⅠ.After this process ,the gene fragments will have one overhang at both ends.The overhangs will help them splice other fragments. </strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 216: | Line 216: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | <small class="text-navy"><div class="text_font">9:00</div></small> | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We purify our genes from the restriction enzyme systems.</strong> | <div class="m-b-xs text_font"><strong>We purify our genes from the restriction enzyme systems.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 231: | Line 231: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | <small class="text-navy"><div class="text_font">in the morning</div></small> | + | <small class="text-navy"><p><div class="text_font">in the morning</div></small></p> |
</div> | </div> | ||
| Line 238: | Line 238: | ||
<div class="m-b-xs text_font"><strong>We cut our plasmids psb3c1 with BsaⅠ.</strong> | <div class="m-b-xs text_font"><strong>We cut our plasmids psb3c1 with BsaⅠ.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 247: | Line 247: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | <small class="text-navy"><div class="text_font">in the morning</div></small> | + | <small class="text-navy"><p><div class="text_font">in the morning</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| Line 254: | Line 254: | ||
a kit box.</strong> | a kit box.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 263: | Line 263: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | <small class="text-navy"><div class="text_font">13:30</div></small> | + | <small class="text-navy"><p><div class="text_font">13:30</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We purify our parts using a kit box.</strong> | <div class="m-b-xs text_font"><strong>We purify our parts using a kit box.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 278: | Line 278: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | <small class="text-navy"><div class="text_font">in the afternoon</div></small> | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| Line 285: | Line 285: | ||
make sure there will be no loops in the part.</strong> | make sure there will be no loops in the part.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 294: | Line 294: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | <small class="text-navy"><div class="text_font">in the afternoon</div></small> | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We purify the parts.</strong> | <div class="m-b-xs text_font"><strong>We purify the parts.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 309: | Line 309: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | <small class="text-navy"><div class="text_font">in the afternoon</div></small> | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We splice our parts with plasmids.</strong> | <div class="m-b-xs text_font"><strong>We splice our parts with plasmids.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 324: | Line 324: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | <small class="text-navy"><div class="text_font">in the afternoon</div></small> | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We prepare LB broth.</strong> | <div class="m-b-xs text_font"><strong>We prepare LB broth.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 339: | Line 339: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | <small class="text-navy"><div class="text_font">19:00</div></small> | + | <small class="text-navy"><p><div class="text_font">19:00</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We transform our plasmids into DH5α.</strong> | <div class="m-b-xs text_font"><strong>We transform our plasmids into DH5α.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 354: | Line 354: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.4</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | <small class="text-navy"><div class="text_font">in the evening</div></small> | + | <small class="text-navy"><p><div class="text_font">in the evening</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We inoculate Ecoli DH5α on LB plates and in media.</strong> | <div class="m-b-xs text_font"><strong>We inoculate Ecoli DH5α on LB plates and in media.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 369: | Line 369: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.5</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.5</div></p> |
| − | <small class="text-navy"><div class="text_font">13:00</div></small> | + | <small class="text-navy"><p><div class="text_font">13:00</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We check the growth of Ecoli on LB plates.</strong> | <div class="m-b-xs text_font"><strong>We check the growth of Ecoli on LB plates.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
No conlony was observed. | No conlony was observed. | ||
So we will use the Ecoli in media to do the following experiments. | So we will use the Ecoli in media to do the following experiments. | ||
| − | + | </div></p> | |
| − | </div> | + | |
</div> | </div> | ||
</div> | </div> | ||
| Line 386: | Line 385: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.5</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.5</div></p> |
| − | <small class="text-navy"><div class="text_font">in the afternoon</div></small> | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We extract plasmids from the solution.</strong> | <div class="m-b-xs text_font"><strong>We extract plasmids from the solution.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 401: | Line 400: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.6</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.6</div></p> |
| − | <small class="text-navy"><div class="text_font">9:00</div></small> | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We prepare Arabidopsis protoplasts.</strong> | <div class="m-b-xs text_font"><strong>We prepare Arabidopsis protoplasts.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
The concentration of protoplasts is too low,this is because we chose the wrong filter paper. | The concentration of protoplasts is too low,this is because we chose the wrong filter paper. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 416: | Line 415: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.6</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.6</div></p> |
| − | <small class="text-navy"><div class="text_font">13:00</div></small> | + | <small class="text-navy"><p><div class="text_font">13:00</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We prepare protoplasts again.</strong> | <div class="m-b-xs text_font"><strong>We prepare protoplasts again.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it.The concentration is much higher,and there is much more sediment of protoplast after centrifuging. | We did it.The concentration is much higher,and there is much more sediment of protoplast after centrifuging. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 431: | Line 430: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.6</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.6</div></p> |
| − | <small class="text-navy"><div class="text_font">in the afternoon</div></small> | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| Line 439: | Line 438: | ||
</strong> | </strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 448: | Line 447: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.7</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.7</div></p> |
| − | <small class="text-navy"><div class="text_font">9:00</div></small> | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>We extract RNA from protoplasts using a kit box.</strong> | <div class="m-b-xs text_font"><strong>We extract RNA from protoplasts using a kit box.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 463: | Line 462: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.7</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.7</div></p> |
| − | <small class="text-navy"><div class="text_font">in the morning</div></small> | + | <small class="text-navy"><p><div class="text_font">in the morning</div></small></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| Line 471: | Line 470: | ||
</strong> | </strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We failed to get any hhl1. There may be something wrong with the process of transformation. | We failed to get any hhl1. There may be something wrong with the process of transformation. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 480: | Line 479: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i><div class="text_font"> 2016.8.10</div> | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.10</div></p> |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
<div class="m-b-xs text_font"><strong>PCR our target DNA fragments:cop1,pif1,phyB,hhl1,pHhl1-hhl1,flu.</strong> | <div class="m-b-xs text_font"><strong>PCR our target DNA fragments:cop1,pif1,phyB,hhl1,pHhl1-hhl1,flu.</strong> | ||
</div> | </div> | ||
| − | <div class="text_font"> | + | <p><div class="text_font"> |
We will check the result tomorrow. | We will check the result tomorrow. | ||
| − | </div> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 13:13, 30 September 2016
Day note
2016.7.31
9:00
Extract genome from Arabidopsis Thaliana
The genome has been degraded greatly,possibly because we didn’t grind the leaves in very low temperature to inhibit the nuclease.We have to do it again.
2016.7.31
13:30
Extract genome from Arabidopsis Thaliana
We extracted it the genome successfully.But the genome has still been degraded a little. (Since we don’t have liquid nitrogen in our lab,so we grinded the leaves in an ice box. )We will use the genome to do the following PCR.
2016.8.1
9:00
We use PCR to generate and to amplify our target DNA fragments:promotercop1,promoter phyB,promoterpif1,gene hhl1,gene pHhl,gene flu.
We failed to get all our target DNA fragments .We will check the primers and the PCR program ,and then do it again.
2016.8.2
9:00
Do the PCR again to get our target DNA fragments.
We PCR gene pHhl1,gene hhl1,gene flu successfully.
We will PCR cop1 together with pif1,phyB tomorrow.
2016.8.3
9:00
We use PCR to get cop1,phyB and pif1
In the first PCR we get cop 1 and pif1,we get higher concentration of phyB in the second time PCR.
2016.8.3
13:30
We use a kit box to purify our PCR products,so we can get our genes.
We did it.
2016.8.3
in the afternoon
We cut our DNA fragments using restriction enzymes:BsaⅠ,PstⅠ,
EcorⅠ.After this process ,the gene fragments will have one overhang at both ends.The overhangs will help them splice other fragments.
We did it.
2016.8.4
9:00
We purify our genes from the restriction enzyme systems.
We did it.
2016.8.4
in the morning
We cut our plasmids psb3c1 with BsaⅠ.
We did it.
2016.8.4
in the morning
We splice our DNA fragments to get the parts we want using
a kit box.
We did it.
2016.8.4
13:30
We purify our parts using a kit box.
We did it.
2016.8.4
in the afternoon
We cut our parts with BsaⅠto
make sure there will be no loops in the part.
We did it.
2016.8.4
in the afternoon
We purify the parts.
We did it.
2016.8.4
in the afternoon
We splice our parts with plasmids.
We did it.
2016.8.4
in the afternoon
We prepare LB broth.
We did it.
2016.8.4
19:00
We transform our plasmids into DH5α.
We did it.
2016.8.4
in the evening
We inoculate Ecoli DH5α on LB plates and in media.
We did it.
2016.8.5
13:00
We check the growth of Ecoli on LB plates.
No conlony was observed.
So we will use the Ecoli in media to do the following experiments.
2016.8.5
in the afternoon
We extract plasmids from the solution.
We did it.
2016.8.6
9:00
We prepare Arabidopsis protoplasts.
The concentration of protoplasts is too low,this is because we chose the wrong filter paper.
2016.8.6
13:00
We prepare protoplasts again.
We did it.The concentration is much higher,and there is much more sediment of protoplast after centrifuging.
2016.8.6
in the afternoon
We transfect our plasmids into the protoplasts，and incubate
the protoplasts in the following light intensity (lux):0,3960,7920,11880.
We did it.
2016.8.7
9:00
We extract RNA from protoplasts using a kit box.
We did it.
2016.8.7
in the morning
We use semiquantitive RT-PCR
to amplify hhl1 to see how much hhl1 is transcripted into mRNA.
We failed to get any hhl1. There may be something wrong with the process of transformation.
2016.8.10
PCR our target DNA fragments:cop1,pif1,phyB,hhl1,pHhl1-hhl1,flu.
We will check the result tomorrow.
