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Revision as of 17:32, 1 October 2016

Enzyme cleavage

Place:Biology Experiment Teaching Center Of Sun Yat-sen University

Date:July 27th, 2016

Experimental drugs: PCR products, Pst1restriction enzyme,10×buffer solution, ddH2O

Procedure:

  1. enzyme cleavage:configuration
    PCR products (DNA) 5 μl
    10×buffer solution 10 μl
    Pst1restriction enzyme 0.8μl
    ddH2O 84.2 μl
    Blending, keeping in 37℃ for 1.5-3h

  2. electrophoresis
    Take out 5μ DNA after enzyme cleavage to electrophoresis.