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− | <p> | + | <p> <h2><b>Project Design</b></h2> |
+ | The BeeT project consists of multiple parts, every student has their own part. We tried to integrate all the modelling with the wet lab. Our project has three main categories, sensing the <i>Varroa destructor</i>, toxin production, and safety. | ||
+ | |||
+ | <h2><b>Wet lab</b></h2> | ||
+ | <i>Lisa</i> searched for the Bt toxin inside the Varroa, <i>Linea</i> made a library of the toxin and <i>Jaccoline</i> made vesicles. <i>Carina</i> made the Varroa sensing system based on sensing mite poop and Vitamine B12. <i>Thomas</i> made a quorum sensing system and a subpopulation system, based on a toxin/anti-toxin sytem. <i>Belwina</i> used Cas9 to improve a biocontainment killswitch. <i>Marijn</i> made the light kill switch in the lab, and <i>Tianhe</i> took care of making a toggle switch and combining the light kill switch and sensing system together. | ||
+ | <h2><b>Modelling</b></h2> | ||
+ | <i>Mario</i> modelled the light kill switch which <i>Marijn</i> was making in the lab. <i>Remco</i> modelled the interaction between the Varroa and Bees and the spread of toxin through the hive. <i>Angelina</i> modelled the quorum sensing subpopulation system and <i>Ronald</i> worked on the metabolic modelling and a in vitro toxicity assay | ||
+ | <h2><b>Safety</b></h2> | ||
+ | As for the safety we want to implement a light kill switch in our end product. Also the Cas9 should prevent the bacteria from spreading through the environment. | ||
+ | |||
+ | <h2><b>Human Practice</b></h2> | ||
+ | <i>Carina</i> is our Captain Human Practice, <i>Carina</i> has a Human Practice team with, <i>Linea</i> and <i>Belwina</i>, who help her with the Human Practice. As one of the first things that have been done for the Human practices is getting in contact with Beekeepers. Together with them we tried to think of a solution for the dying bees. We have a collaboration with <b>Synenergene</b> where an application scenario was generated. A movie about synthetic biology has been made for the <b>RIVM</b>. | ||
+ | |||
+ | <b>Wiki Construction</b> | ||
+ | <i>Lisa</i> is the Wiki Captain, together with <i>Ronald</i>, <i>Remco</i> and <i>Tianhe</i> the design of the wiki was made. With the backbone of the <b>iGEM Wageningen_UR 2014</b> we made our team page. <i>Lisa</i> made all the illustrations herself and together with the guys did most of the programming work. | ||
+ | |||
+ | <h2>Finances</h2> | ||
+ | With <i>Angelina</i> as our Treasurer and together with <i>Jaccoline</i> for the crowdfunding and <i>Mario</i> for the plane tickets and accommodation. They searched for sponsors. | ||
+ | |||
+ | <h2>Other Team Roles</h2> | ||
+ | With <i>Thomas</i> as our Team Captain the whole team functioned well. Off course he had much help from his secretary <i>Belwina</i>. | ||
+ | <i>Linea</i> Captain Facebook, nothing is more important these days than presenting yourself at social media. | ||
+ | <i>Marijn</i> our lab safety manager took care of keeping the lab save and clean. | ||
+ | |||
+ | <h2>Supervisors</h2> | ||
+ | Christian Fleck, John van der Oost, Robert Smith, Kees van der Ark, Maarten Reijnders, Stamatios Damalas, Martha Viar Vazquez, Emma Keizer, Alex Kruis, Franklin Nobrega de Luzia. | ||
+ | <b>Advisors</b> | ||
+ | Bob Mulder | ||
</p> | </p> | ||
</section> | </section> |
Revision as of 14:31, 5 October 2016
Attributions