Difference between revisions of "Team:Wageningen UR/Model"

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<h1><b>Overview</b></h1>
 
<h1><b>Overview</b></h1>
 
<p>ADD TEXT HERE</p>
 
<p>ADD TEXT HERE</p>
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<h1><b>Quorum Sensing</b></h1>
 
<h1><b>Quorum Sensing</b></h1>
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<p><b> Introduction</b><br>
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For the iGEM project a toxin producing system has been made. We wanted to create a system where bacteria can produce toxin in waves and hereby create different cell populations. With the use of quorum sensing and a toxin/anti-toxin system, as shown in Figure 1, we expect to find different cell populations.
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<p><b> What is quorum sensing</b><br>
 
<p><b> What is quorum sensing</b><br>
 
Quorum sensing is a cell-cell communication system. The detection of chemical molecules allows the bacteria to distinguish between low and high cell densities, in this way control gene expression in response to changes in cell number <sup> <a href="#fn1" id="ref1">1</a></sup>.   
 
Quorum sensing is a cell-cell communication system. The detection of chemical molecules allows the bacteria to distinguish between low and high cell densities, in this way control gene expression in response to changes in cell number <sup> <a href="#fn1" id="ref1">1</a></sup>.   

Revision as of 12:32, 6 October 2016

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Wageningen UR iGEM 2016

 

Overview

ADD TEXT HERE

Quorum Sensing

Introduction
For the iGEM project a toxin producing system has been made. We wanted to create a system where bacteria can produce toxin in waves and hereby create different cell populations. With the use of quorum sensing and a toxin/anti-toxin system, as shown in Figure 1, we expect to find different cell populations.

What is quorum sensing
Quorum sensing is a cell-cell communication system. The detection of chemical molecules allows the bacteria to distinguish between low and high cell densities, in this way control gene expression in response to changes in cell number 1.

Methods
During the research Matlab version R2016a has been used.
Because there was no data from the wet lab we assumed that all the parameters in the system were random. The parameters are all obtained by latin hypercube Latin hypercube is a statistical method to get random numbers from a box of n by n numbers. For example x = 4 with x is divisions and n = 2 with n is number of samples. You will obtain a box with 4 square times 2 square, give you 24 random numbers. For each parameter one number out of this box is randomly chosen. samples.

How could quorum sensing develop spatial inhomogeneities in toxin/anti-toxin systems?
When quorum sensing ensures that the toxin is only produced when the density of bacteria is high enough to produce significant amounts of toxin, this ‘standardizes’ the amount of toxin produced by the bacteria. The toxin/anti-toxin system will be coupled to the quorum sensing system. Together, quorum sensing and forming of non-producing subpopulations by the toxin/anti-toxin system, allow bacteria to produce ‘waves’ of toxin.

Results

Figure x. EXAMPLE TEXT.

Quorum sensing system

References

    1. Eri Nasuno, Nobutada Kimura, Masaki J. Fujita, Cindy H. Nakatsu, Yoichi Kamagata, and Satoshi Hanada (2012). Phylogenetically Novel LuxI/LuxR-Type Quorum Sensing Systems Isolated Using a Metagenomic Approach Vol, 78, number 22. ↩

    Light Kill Switch

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    Metabolic Model

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    Figure x. EXAMPLE TEXT.

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