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| | <ul role="menu" class="dropdown-menu"> | | <ul role="menu" class="dropdown-menu"> |
| | <li><a href="https://2016.igem.org/GDSYZX-United/TEAM/team_members">Team members</a></li> | | <li><a href="https://2016.igem.org/GDSYZX-United/TEAM/team_members">Team members</a></li> |
| | + | <li><a href="https://2016.igem.org/GDSYZX-United/TEAM/collarboration">Collarboration</a></li> |
| | + | <li><a href="https://2016.igem.org/GDSYZX-United/TEAM/attribution">Attribution</a></li> |
| | </ul> | | </ul> |
| | </li> | | </li> |
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| | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/enzyme_cleavage">Enzyme cleavage</a></li> | | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/enzyme_cleavage">Enzyme cleavage</a></li> |
| | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/preparation_arabidopsis_protoplast">Preparation of the Arabidopsis protoplast</a></li> | | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/preparation_arabidopsis_protoplast">Preparation of the Arabidopsis protoplast</a></li> |
| − | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/result">Result</a></li> | + | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/result">Result</a></li> |
| | </ul> | | </ul> |
| | </li> | | </li> |
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| | <ul role="menu" class="dropdown-menu"> | | <ul role="menu" class="dropdown-menu"> |
| | <li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/lab_safety">Lab safety</a></li> | | <li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/lab_safety">Lab safety</a></li> |
| − | <li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/safety_of_process">Safety_of_process</a></li> | + | <li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/safety_of_process">Safety of process</a></li> |
| | </ul> | | </ul> |
| | </li> | | </li> |
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| | </ul> | | </ul> |
| | </div> | | </div> |
| − | </nav> | + | </nav> |
| | + | |
| | <div class="row animated fadeInRight"> | | <div class="row animated fadeInRight"> |
| | <div class="col-sm-12"> | | <div class="col-sm-12"> |
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| | </body> | | </body> |
| | </html> | | </html> |
| − | <html>
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| − | <meta charset="utf-8">
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| − | <meta name="viewport" content="width=device-width, initial-scale=1.0">
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| − | <link href="https://2016.igem.org/Template:GDSY<html>
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| − | <link href="https://2016.igem.org/Template:GDSYZX-United/CSS/bootstrap_min14ed?action=raw&ctype=text/css&v=3.3.6" rel="stylesheet">
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| − | <link href="https://2016.igem.org/Template:GDSYZX-United/CSS/font-awesome_min93e3?action=raw&ctype=text/css&v=4.4.0" rel="stylesheet">
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| − | <link href="https://2016.igem.org/Template:GDSYZX-United/CSS/animate_min?action=raw&ctype=text/css" rel="stylesheet">
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| − | <link href="https://2016.igem.org/Template:GDSYZX-United/CSS/style_min862f?action=raw&ctype=text/css&v=4.1.0" rel="stylesheet">
| |
| − |
| |
| − | <style type="text/css">
| |
| − | #sideMenu, #top_title {display:none;}
| |
| − | #content { padding:0px; width:100%; margin-top:0px; margin-left:0px;}
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| − | #content,body {
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| − | background: -webkit-linear-gradient(left,rgba(195,243,219,1),rgba(228,249,234,0.1)); /* Safari 5.1 - 6 */
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| − | background: -o-linear-gradient(right,rgba(195,243,219,1),rgba(228,249,234,0.1)); /* Opera 11.1 - 12*/
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| − | background: -moz-linear-gradient(right,rgba(195,243,219,1),rgba(228,249,234,0.1)); /* Firefox 3.6 - 15*/
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| − | background: linear-gradient(to right, rgba(195,243,219,1), rgba(228,249,234,0.1)); /* 标准的语法 */
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| − | padding-top:10px;
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| − | }
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| − | nav {padding-top:10px;}
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| − | .text_font {
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| − | line-height:120%;
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| − | font-size:130%
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| − | }
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| − | </style>
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| − | </head>
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| − |
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| − | <body>
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| − | <div class="wrapper wrapper-content">
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| − | <nav class="navbar navbar-fixed-top navbar-inverse" role="navigation">
| |
| − | <!-- <div class="navbar-header">
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| − | <a href="#" class="navbar-brand">GDSYZX-United</a>
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| − | </div> -->
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| − | <div class="navbar-collapse collapse" id="navbar">
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| − | <ul class="nav navbar-nav navbar-right">
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| − | <li class="dropdown">
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| − | <a aria-expanded="false" role="button" href="https://2016.igem.org/Team:GDSYZX-United"> HOME </a>
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| − | </li>
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| − | <li class="dropdown">
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| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> TEAM <span class="caret"></span></a>
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| − | <ul role="menu" class="dropdown-menu">
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| − | <li><a href="https://2016.igem.org/GDSYZX-United/TEAM/team_members">Team members</a></li>
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| − | </ul>
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| − | </li>
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| − | <li class="dropdown">
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| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> PROJECT <span class="caret"></span></a>
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| − | <ul role="menu" class="dropdown-menu">
| |
| − | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/project_description">Project description</a></li>
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| − | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/extract _dna">Extract DNA in arabidopsis thaliana</a></li>
| |
| − | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/enzyme_cleavage">Enzyme cleavage</a></li>
| |
| − | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/preparation_arabidopsis_protoplast">Preparation of the Arabidopsis protoplast</a></li>
| |
| − | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/result">Result</a></li>
| |
| − | </ul>
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| − | </li>
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| − | <li class="dropdown">
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| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> PARTS <span class="caret"></span></a>
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| − | <ul role="menu" class="dropdown-menu">
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| − | <li><a href="https://2016.igem.org/GDSYZX-United/PARTS/parts">Parts</a></li>
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| − | </ul>
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| − | </li>
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| − | <li class="dropdown">
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| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> NOTEBOOK <span class="caret"></span></a>
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| − | <ul role="menu" class="dropdown-menu">
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| − | <li><a href="https://2016.igem.org/GDSYZX-United/NOTEBOOK/day_note">Day notes</a></li>
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| − | <li><a href="https://2016.igem.org/GDSYZX-United/NOTEBOOK/procedure_record">Procedure record</a></li>
| |
| − | <li><a href="https://2016.igem.org/GDSYZX-United/NOTEBOOK/protocol">Protocol</a></li>
| |
| − | </ul>
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| − | </li>
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| − | <li class="dropdown">
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| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> HUMAN PRACTICE <span class="caret"></span></a>
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| − | <ul role="menu" class="dropdown-menu">
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| − | <li><a href="https://2016.igem.org/GDSYZX-United/HUMAN_PRACTICE/human_practice">Human Practice</a></li>
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| − | <li><a href="https://2016.igem.org/GDSYZX-United/HUMAN_PRACTICE/album">Album</a></li>
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| − | </ul>
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| − | </li>
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| − | <li class="dropdown">
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| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> SAFETY <span class="caret"></span></a>
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| − | <ul role="menu" class="dropdown-menu">
| |
| − | <li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/lab_safety">Lab safety</a></li>
| |
| − | <li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/safety_of_process">Safety_of_process</a></li>
| |
| − | </ul>
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| − | </li>
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| − | <li class="dropdown">
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| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> ENTREPRENEURSHIP <span class="caret"></span></a>
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| − | <ul role="menu" class="dropdown-menu">
| |
| − | <li><a href="#">1</a></li>
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| − | <li><a href="#">2</a></li>
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| − | <li><a href="#">3</a></li>
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| − | <li><a href="#">4</a></li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </nav>
| |
| − | <div class="row animated fadeInRight">
| |
| − | <div class="col-sm-12">
| |
| − | <div class="ibox float-e-margins">
| |
| − | <div class="timeline">
| |
| − | <div class="timeline-item">
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| − | <div class="row">
| |
| − | <div class="col-xs-12 text-center">
| |
| − | <h1>Day note</h1>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.7.31</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>Extract genome from Arabidopsis Thaliana</strong></div>
| |
| − | <p><div class="text_font">The genome has been degraded greatly,possibly because we didn’t grind the leaves in very low temperature to inhibit the nuclease.We have to do it again.</div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.7.31</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">13:30</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>Extract genome from Arabidopsis Thaliana</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We extracted it the genome successfully.But the genome has still been degraded a little. (Since we don’t have liquid nitrogen in our lab,so we grinded the leaves in an ice box. )We will use the genome to do the following PCR.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.1</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We use PCR to generate and to amplify our target DNA fragments:promotercop1,promoter phyB,promoterpif1,gene hhl1,gene pHhl,gene flu.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We failed to get all our target DNA fragments .We will check the primers and the PCR program ,and then do it again.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.2</div></p>
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| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>Do the PCR again to get our target DNA fragments.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We PCR gene pHhl1,gene hhl1,gene flu successfully.
| |
| − | We will PCR cop1 together with pif1,phyB tomorrow.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p>
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| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We use PCR to get cop1,phyB and pif1</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | In the first PCR we get cop 1 and pif1,we get higher concentration of phyB in the second time PCR.
| |
| − | </div></p>
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| − | </div>
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| − | </div>
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| − | </div>
| |
| − | <div class="timeline-item">
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| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p>
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| − | <small class="text-navy"><p><div class="text_font">13:30</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We use a kit box to purify our PCR products,so we can get our genes.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
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| − | </div>
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| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p>
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| − | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p>
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| − | </div>
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| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We cut our DNA fragments using restriction enzymes:BsaⅠ,PstⅠ,
| |
| − | EcorⅠ.After this process ,the gene fragments will have one overhang at both ends.The overhangs will help them splice other fragments. </strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We purify our genes from the restriction enzyme systems.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">in the morning</div></small></p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We cut our plasmids psb3c1 with BsaⅠ.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">in the morning</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We splice our DNA fragments to get the parts we want using
| |
| − | a kit box.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">13:30</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We purify our parts using a kit box.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We cut our parts with BsaⅠto
| |
| − | make sure there will be no loops in the part.</strong>
| |
| − | </div>
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| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p>
| |
| − | </div>
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| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We purify the parts.</strong>
| |
| − | </div>
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| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We splice our parts with plasmids.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We prepare LB broth.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
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| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">19:00</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We transform our plasmids into DH5α.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
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| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">in the evening</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We inoculate Ecoli DH5α on LB plates and in media.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.5</div></p>
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| − | <small class="text-navy"><p><div class="text_font">13:00</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We check the growth of Ecoli on LB plates.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | No conlony was observed.
| |
| − | So we will use the Ecoli in media to do the following experiments.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.5</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
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| − | <div class="m-b-xs text_font"><strong>We extract plasmids from the solution.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
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| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.6</div></p>
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| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
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| − | </div>
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| − | <div class="col-xs-8 content">
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| − | <div class="m-b-xs text_font"><strong>We prepare Arabidopsis protoplasts.</strong>
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| − | </div>
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| − | <p><div class="text_font">
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| − | The concentration of protoplasts is too low,this is because we chose the wrong filter paper.
| |
| − | </div></p>
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| − | </div>
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| − | </div>
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| − | </div>
| |
| − | <div class="timeline-item">
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| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.6</div></p>
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| − | <small class="text-navy"><p><div class="text_font">13:00</div></small></p>
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| − | </div>
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| − | <div class="col-xs-8 content">
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| − | <div class="m-b-xs text_font"><strong>We prepare protoplasts again.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.The concentration is much higher,and there is much more sediment of protoplast after centrifuging.
| |
| − | </div></p>
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| − | </div>
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| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.6</div></p>
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| − | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p>
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| − | </div>
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| − | <div class="col-xs-8 content">
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| − | <div class="m-b-xs text_font"><strong>We transfect our plasmids into the protoplasts，and incubate
| |
| − | the protoplasts in the following light intensity (lux):0,3960,7920,11880.
| |
| − | </strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
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| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.7</div></p>
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| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
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| − | </div>
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| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We extract RNA from protoplasts using a kit box.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.7</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">in the morning</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We use semiquantitive RT-PCR
| |
| − | to amplify hhl1 to see how much hhl1 is transcripted into mRNA.
| |
| − | </strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We failed to get any hhl1. There may be something wrong with the process of transformation.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.10</div></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>PCR our target DNA fragments:cop1,pif1,phyB,hhl1,pHhl1-hhl1,flu.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We will check the result tomorrow.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <li><a href="#">Project description</a></li>
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| − | <li><a href="#">制取拟南芥DNA</a></li>
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| − | <li><a href="#">酶切</a></li>
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| − | <li><a href="#">制取拟南芥原生质体</a></li>
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| − | <li><a href="#">result</a></li>
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| − | <li><a href="#">跑胶图片</a></li>
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| − | <li><a href="https://2016.igem.org/GDSYZX-United/NOTEBOOK/day_note">Day notes</a></li>
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| − | <li><a href="https://2016.igem.org/GDSYZX-United/NOTEBOOK/procedure_record">Procedure record</a></li>
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| − | <li><a href="https://2016.igem.org/GDSYZX-United/NOTEBOOK/protocol">Protocol</a></li>
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| − | <li><a href="#">1</a></li>
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| − | <h1>Day note</h1>
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| − | </div>
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| − | </div>
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| − | <div class="timeline-item">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.7.31</div></p>
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| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
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| − | </div>
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| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>Extract genome from Arabidopsis Thaliana</strong></div>
| |
| − | <p><div class="text_font">The genome has been degraded greatly,possibly because we didn’t grind the leaves in very low temperature to inhibit the nuclease.We have to do it again.</div></p>
| |
| − | </div>
| |
| − | </div>
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| − | </div>
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| − | <div class="timeline-item">
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| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.7.31</div></p>
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| − | <small class="text-navy"><p><div class="text_font">13:30</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>Extract genome from Arabidopsis Thaliana</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We extracted it the genome successfully.But the genome has still been degraded a little. (Since we don’t have liquid nitrogen in our lab,so we grinded the leaves in an ice box. )We will use the genome to do the following PCR.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.1</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We use PCR to generate and to amplify our target DNA fragments:promotercop1,promoter phyB,promoterpif1,gene hhl1,gene pHhl,gene flu.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We failed to get all our target DNA fragments .We will check the primers and the PCR program ,and then do it again.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
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| − | <div class="timeline-item">
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| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.2</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>Do the PCR again to get our target DNA fragments.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We PCR gene pHhl1,gene hhl1,gene flu successfully.
| |
| − | We will PCR cop1 together with pif1,phyB tomorrow.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
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| − | <div class="timeline-item">
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| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We use PCR to get cop1,phyB and pif1</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | In the first PCR we get cop 1 and pif1,we get higher concentration of phyB in the second time PCR.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
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| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p>
| |
| − | <small class="text-navy"><p><div class="text_font">13:30</div></small></p>
| |
| − | </div>
| |
| − | <div class="col-xs-8 content">
| |
| − | <div class="m-b-xs text_font"><strong>We use a kit box to purify our PCR products,so we can get our genes.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="timeline-item">
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| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p>
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| − | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p>
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| − | </div>
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| − | <div class="col-xs-8 content">
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| − | <div class="m-b-xs text_font"><strong>We cut our DNA fragments using restriction enzymes:BsaⅠ,PstⅠ,
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| − | EcorⅠ.After this process ,the gene fragments will have one overhang at both ends.The overhangs will help them splice other fragments. </strong>
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| − | </div>
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| − | <p><div class="text_font">
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| − | We did it.
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| − | </div></p>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="timeline-item">
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| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
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| − | <small class="text-navy"><p><div class="text_font">9:00</div></small></p>
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| − | </div>
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| − | <div class="col-xs-8 content">
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| − | <div class="m-b-xs text_font"><strong>We purify our genes from the restriction enzyme systems.</strong>
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| − | </div>
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| − | <p><div class="text_font">
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| − | We did it.
| |
| − | </div></p>
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| − | </div>
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| − | </div>
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| − | </div>
| |
| − | <div class="timeline-item">
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| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
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| − | <small class="text-navy"><p><div class="text_font">in the morning</div></small></p>
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| − | </div>
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| − |
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| − | <div class="col-xs-8 content">
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| − | <div class="m-b-xs text_font"><strong>We cut our plasmids psb3c1 with BsaⅠ.</strong>
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| − | </div>
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| − | <p><div class="text_font">
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| − | We did it.
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| − | </div></p>
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| − | </div>
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| − | </div>
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| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
| |
| − | <div class="col-xs-3 date">
| |
| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
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| − | <small class="text-navy"><p><div class="text_font">in the morning</div></small></p>
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| − | </div>
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| − | <div class="col-xs-8 content">
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| − | <div class="m-b-xs text_font"><strong>We splice our DNA fragments to get the parts we want using
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| − | a kit box.</strong>
| |
| − | </div>
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| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
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| − | </div>
| |
| − | </div>
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| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p>
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| − | <small class="text-navy"><p><div class="text_font">13:30</div></small></p>
| |
| − | </div>
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| − | <div class="col-xs-8 content">
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| − | <div class="m-b-xs text_font"><strong>We purify our parts using a kit box.</strong>
| |
| − | </div>
| |
| − | <p><div class="text_font">
| |
| − | We did it.
| |
| − | </div></p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="timeline-item">
| |
| − | <div class="row">
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| − | <div class="col-xs-3 date">
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| − | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.
| |
