Difference between revisions of "Team:Wageningen UR/Notebook"
| Line 58: | Line 58: | ||
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/7f/T--Wageningen_UR--upbutton.jpg"/> | ||
| − | <h3 style="color:#6d5d31">Optogenetic Kill Switch</h3> <!--Change text color here--> | + | <h3 style="color:#6d5d31 font-size:18px">Optogenetic Kill Switch</h3> <!--Change text color here--> |
</div> | </div> | ||
<p>The light-induced kill switch was one of our biocontainment methods.</p> | <p>The light-induced kill switch was one of our biocontainment methods.</p> | ||
Revision as of 14:48, 14 October 2016
Find the documentation of our research here.
-
Protein Engineering
We wanted to engineer Cry3Aa to become toxic to Varroa destructor.
-
In Vitro Assay
To provide an alternative for tests on live Varroa mites, we designed an assay that uses brush border membrane vesicles.
-
Varroa isolates
We tried to find our own Varroa entomopathogen.
-
Riboswitches
To detect the presence of Varroa mites in the beehive, we made a B12 and a guanine riboswitch.
-
Quorum Sensing
A quorum sensing system was characterized to regulate toxin production in BeeT.
-
Optogenetic Kill Switch
The light-induced kill switch was one of our biocontainment methods.
-
Modelling
How we modelled .
-
Chitinases
As a back-up, we wanted to use chitinases which are more harmful to Varroa destructor than honeybees.
-
Cas9-based Kill Switch
The Cas9-based kill switch was one of our biocontainment methods.
-
Toxin Scanner
We made a software tool for finding new Cry proteins toxic to Varroa destructor.
-
Metabolic Modelling
We wanted to use a metabolic model to see the effect of our BeeT distribution method.
-
Testing E. coli survival
Following the results of the Metabolic Model, we needed more data.
