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Design
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<div class="h1_font_size">Gold</div>
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<div class="h2_font_size">1. Vector for genes stacking </div>
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<div class="p_font_size">Our human practice work involved several questions relating to bio-safety,social justice and public acceptance.Our astarice is actually a genetically modified organism,so our work focused on people’s concern about GMO.</div>
    <div class="p_font_size" style="text-indent:0em">PSY (phytoene synthase) catalyzes Geranylgeranyl-PP into Phytoene. The gene CrtI from Erwinia uredovora could finish the catalysis process from Phytoene to Lycopene. Therefore, when these two genes(PSY and CrtI) with specific promoters of endosperm ahead ,they will express CRTISO and β-LCY enzymes which synthesizeβ-Carotene to produce the famous Golden Rice. There are still two steps from β-Carotene to astaxanthin: BHY (β-carotenehy droxylase) catalyze β-Carotene to Zeaxanthin. And BKT catalyzes directly to synthesize the end product astaxnthin. The expression of  the endogenous gene BHY in rice is still unknown, accordingly it needs at least 3 genes (PSY+Crt I+BKT,BPC) or 4 genes(PSY+Crt I+BKT+BHY, BBPC) to synthesize astaxanthin. For the combination of three genes, if the endogenous gene BHY of rice has little expression, maybe there will be just a little astaxanthin produced(even nothing!). But for the combination of four genes, it will create a complete metabolic pathway for astaxanthin production. Surely, it could produce astaxanthin. Thus we use the systems of Assembly of multiple genes and transformation, and the specific promoters of endosperm to construct three vectors(380-PC,BPC and BBPC) to study the metabolic of the synthesis of astaxanthin in the endosperm of rice. And the four genes we use were  synthesized and codon optimization according to the  preferred codons in rice.
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<div class="h2_font_size">1. Investigation</div>
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To assemble these four genes of astaxanthin biosynthetic pathway in rice endosperm, a modified multigene vector system, TransGene Stacking II (TGSII), was used. This system consists of a transformation-competent artificial chromosome (TAC)-based binary acceptor vector (pYLTAC380GW), together with two donor vectors (pYL322-d1/ pYL322-d2). By using the Cre/loxP recombination system and two pairs of mutant loxP sites, multiple rounds of gene assembly cycles were carried out with alternative use of the donor vectors, and multiple genes were sequentially delivered into the TAC vector. By this way, multiple genes can be easily stacking into a TAC-based binary acceptor vector. (Liu et al., PNAS, 1999, 96: 6535-6540; Lin et al., PNAS, 2003, 100: 5962-5967;Zhu et al., unpublished)You can read more details by click here! part (转到Part栏目)and protocol (转到Notebook 栏目下的protocol栏目)
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<div class="h2_font_size">2.Experimental design</div>
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<div class="p_font_size">We hoped our human practice work can influence more ordinary people.So,above all,we should realize other people's opinion to transgenic food.Because of that,we made a questionnaire about GMO.</div>
<div class="p_font_size">Firstly, the nucleic acid sequences of four genes have been codon optimized and directly synthesized for stable expression in rice. Then, these genes were subcloned into endosperm-specific gene cassettes of two donors. Secondly, these genes and a marker-free elementwere assembled into a TAC-based binary vector by using a transgene stacking II system. Finally, the obtained marker-free multigene vector was transferred into Agrobacterium tumefaciens EHA105 for rice callus transformation. The transgenic plants were identified by analyses of PCR, RT-PCR, qRT-PCR and HPLC. The schematic diagram of our project was shown in Figure 3.
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<div class="p_font_size">The data we’ve obtained in questionnaire is showing below:</div>
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<div class="p_font_size">The respondents’  proportion of men and women is approximately balanced. The number of age group which between 18 from 25 is the majority. What’s more , the respondents have a Bachelor degree or a higher degree.</div>
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<div class="p_font_size">Figure 3 The schematic diagram of Astaxanthin Rice project.
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<h2>Diagram of our project design:
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(1)The sequence of the four transgenes are accessed from Genbank and codon optimized for expression in Orazy sativa.
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<div class="h3_font_size">1.1 The main content</div>
</div><div class="p_font_size">(2)Genes together with expressive elements are integrated into one plasmid utilizing E.coli;
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</div><div class="p_font_size">(3)Construct competent for transformation is transferred into Agrobacterium;
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<div class="p_font_size"><font style="font-weight:bold">Question 4:</font> Do you know what is Genetically modified (gm)?</div>
</div><div class="p_font_size">(4)Multiple genes are delivered into rice genome. And then, culture the transgenic plant and harvest astaxanthin rice!
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<div class="h2_font_size">3.Marker free</div>
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In this part, we mainly introduce the work we used cre/loxp recombination,a site-specific recombinase technology to delete the selective marker.
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One of the reason why transgenetic plant thought to be dangerous is that various selective markers are applied in the process of positive individuals screening. Selective markers utilize in screening to distinct the transgenetic individuals from negative ones while markers are considered to be useless after screening. Both resistant gene and reporter gene, such as hygromycin phosphotransferase and green fluorescent protein, can be used as selective markers. However, resistant gene-based selective markers attracted much more attention due to its biologically potential danger. Questions about resistant gene-based selective markers’ safety can divide into two parts. One is that drug resistance of pathogenic microbes would be obtained through gene drift. Another is products codes by these selective genes would be a new types of allergen in food made from transgenetic plant. Thus, it is necessary to remove the selective markers after screening.
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<div class="p_font_size">Analysis of the Q4:4/5 of the respondents have a certain understanding for "GM technology",1/5 respondents don’t understand this technology or even don’t know. This suggests that the "GM technology" was received by the majority of respondents.</div>
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<div class="p_font_size"><font style="font-weight:bold">Question 6:</font> Will you eat gm food?</div>
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Concerning the potential dangers of selective markers, we design a Cre-loxP based side-direct recombinant system to knock out hygromycin phosphotransferase gene in pollen utilizing Cre recombinase under the regulation of pollen-specific promoter Pv4. After performing selfing, we obtain the marker-free homozygous transgenetic organisms. Though plant had been used in the past iGEM projects, we utilize Orazy Sativa as a chassis and propose a marker-deletion system for the first time in iGEM.  
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<div class="p_font_size">DNA recombination mediated by Cre recombinase has become an important tool to generate marker-free transgentic plants because it is time-specific, tissue-specific and site-specific.
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The Cre/loxP system of bacterio-phage P1 is a site-specific recombination system that consists of two components:the recombinase(Cre)and its recognition sites(loxP).Cre mediates recombination events and causes the excision of the DNA segment between two directly adjacent loxP sites. Besides,The result of recombination depends on the orientation of the loxP sites. For two lox sites on the same chromosome arm, inverted loxP sites will cause an inversion of the intervening DNA, while a direct repeat of loxP sites will cause a deletion event.If loxP sites are on different chromosomes, it is possible fortranslocation events to be catalysed by Cre thus inducing the recombination.
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Figure 4 The schematic diagram of the marker-free element. PV4 is an anther-specific promoter that drives Cre gene expression in anther.
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<div class="p_font_size">Analysis :About a third of the people can accept genetically modified (gm) food. 17% of people determined not to eat genetically modified food.Suggest that the public acceptance for genetically modified foods is improving.</div>
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<div class="p_font_size"><font style="font-weight:bold">Question 11:</font> Is transgenosis beneficial or harmful in your opinions?</div>
If we want to assemble an tissue-specific promoter ahead of the Cre gene, the process of delection could be regulated when we designed a direct repeat of loxP sites with the intervening marker gene. As a result, we can delete the gene wherever it is located on. By using tissue-specific promoter, we have got the marker-free pollens.
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We found Pv4, a rice anther specific promoter. When our transgentic plants grow up, the Cre gene will be expressed which should be drived by Pv4, thus only in the anther of stamens. Hence,we got the marker-free pollens. Through selfing , we can get the homozygote of marker-free transgentic plants.
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<div class="h2_font_size">references</div>
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【1】Varda Mann, Mark Harker, Iris Pecker, and Joseph Hirschberg. Metabolic engineering of astaxanthin production in tobacco flowers. Nature Biotechnology . 18, 888-892 (2002)
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</div><div class="p_font_size">【2】Salim Al-Babili, Peter Beyer. Golden Rice–five years on
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the road–five years to go? Trends in Plant Science. 10, 12, 565-573 (2005)
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</div><div class="p_font_size">【3】Jacqueline A Paine, Catherine A Shipton, Sunandha Chaggar, Rhian M Howells, Mike J Kennedy, Gareth Vernon, Susan Y Wright, Edward Hinchliffe, Jessica L Adams, Aron L Silverstone, Rachel Drake. Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Nature Biotechnology. 23, 4, 482-487 (2005)
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</div><div class="p_font_size">【4】Cong-Ping Tan, Fang-Qing Zhao, Zhong-Liang Su,  Cheng-Wei Liang, Song Qin. Expression of β-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii. J Appl Phycol . 19, 347–355 (2007)
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</div><div class="p_font_size">【5】Giovanni Giuliano. Plant carotenoids: genomics meets multi-gene engineering. Plant Biology. 19, 111–117 (2014)
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</div><div class="p_font_size">【6】Yook Jang Soo, Okamoto Masahiro, Rakwal Randeep, Shibato Junko, Lee Min Chul, Matsui Takashi, Chang Hyukki,  Cho Joon Yong, Soya Hideaki.  
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</div><div class="p_font_size">【7】Astaxanthin supplementation enhances adult hippocampal neurogenesis and spatial memory inmice. Molecular nutrition & food research. 60 , 589-599(2016)
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<div class="p_font_size">Analysis : Most people had skeptical attitude to genetically modification (gm) . For respondents,genetically modification is an emerging technology. The public don’t understand enough about genetically modified crops,so they cannot hold a certain attitude to it. </div>
                       
+
<br>
 +
<div class="p_font_size"><font style="font-weight:bold">Question 12:</font> Will you use gm products (non-food)?</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/2/29/T--SCAU-China--Engagement13.png">
 +
</div>
 +
</div>
 +
<br>
 +
<div class="p_font_size"><font style="font-weight:bold">Question 13:</font> Have you ever heard of skin care product that contains astaxanthin?</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s12" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/a/ab/T--SCAU-China--Engagement14.jpg">
 +
</div>
 +
</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/a/a1/T--SCAU-China--Engagement15.png">
 +
</div>
 +
</div>
 +
<br>
 +
<div class="p_font_size"><font style="font-weight:bold">Question 14:</font>Will you choose skin care product or health food contains astaxanthin (gm) ?</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/a/a5/T--SCAU-China--Engagement16.png">
 +
</div>
 +
</div>
 +
<div class="p_font_size">If new genetically modified foods were put into market, about half of people can fully accept.While the acceptance of gm products (non-food) is even higher . However,our public do not know much about astaxanthin product at present, So we should Enlarge the propaganda. </div>
 +
<div class="p_font_size">According to the data we’ve got from investigation,we can see that most people are holding a neutral opinion on development of GMO,so it’s possible that we can help them to learn more about transgenic and dispel their qualm,thus make more people accept GMO products.So as to reach this aim,we designed a series of interesting activities.</div>
 +
<div class="h2_font_size">2. Spreading knowledge to our society</div>
 +
<div class="h3_font_size">2.1 Writing <font style="font-style:italic;font-weight:">GMO handbook</font></div>
 +
<div class="p_font_size">After participating in several lectures and talks,we learnt a lot.Later we wrote a handbook about GMO as a summary,which contains our answers to many questions about GMO.We can use this as educational material.The list below is content of our handbook.</div>
 +
<div style="background-color:#D4F6E0">
 +
<br>
 +
<div class="h4_font_size" align="center"><font color="navy">the chapter of popular science</font></div>
 +
<div class="h4_font_size"><font color="navy"><small>1.What are you eat</small></font></div>
 +
<div class="p_font_size"><small>1. Brief introduction to genetically modified food that you eat</small></div>
 +
<div class="p_font_size"><small>2. A reasonable concern——glyphosate</small></div>
 +
<div class="p_font_size"><small>3. Better gm products</small></div>
 +
<div class="h4_font_size"><font color="navy"><small>2.Pathological obsession——Opposition to genetically modified</small></font></div>
 +
<div class="p_font_size"><small>1.‘They’ want to confuse you</small></div>
 +
<div class="p_font_size"><small>2. The preliminary victory——Papaya</small></div>
 +
<div class="p_font_size"><small>3. Organic food is not more secure </small></div>
 +
<div class="p_font_size"><small>4. Being hated humanitarian projects——Golden rice</small></div>
 +
<br>
 +
<div class="h4_font_size" align="center"><font color="navy">the chapter of Genetically modified knowledge</font></div>
 +
<div class="h4_font_size"><font color="navy"><small> 1.Basic knowledge of genetically modified</small></font></div>
 +
<div class="p_font_size"><small>1.What is  genetically modified</small></div>
 +
<div class="p_font_size"><small>2.What's the relationship between genetic and biological traits</small></div>
 +
<div class="p_font_size"><small>3.What is a genetically modified  technology and products</small></div>
 +
<div class="h4_font_size"><font color="navy"><small>2.The characteristics of the genetically modified technology</small></font></div>
 +
<div class="p_font_size"><small>1.What is the commonly used genetically modified (gm) technology</small></div>
 +
<div class="p_font_size"><small>2.What similarities and differences between transgenic technology and the traditional breeding</small></div>
 +
<div class="p_font_size"><small>3.What are genetically modified (gm) technology applications</small></div>
 +
<div class="h4_font_size"><font color="navy"><small>3.The development of genetically modified</small></font></div>
 +
<div class="p_font_size"><small>1.How is Genetically modified that produce and develop?</small></div>
 +
<div class="p_font_size"><small>2.What are genetically modified  research direction in the global</small></div>
 +
<div class="p_font_size"><small>3.What is the matter of the industrialization of the genetically modified organisms</small></div>
 +
<div class="p_font_size"><small>4.Genetically modified technology development present situation</small></div>
 +
<br>
 +
<div class="h4_font_size" align="center"><font color="navy">the chapter of Safety regulation</font></div>
 +
<div class="h4_font_size"><font color="navy"><small>1.Genetically modified product safety</small></font></div>
 +
<div class="p_font_size"><small>1.what aspects that Genetically modified product safety involves</small></div>
 +
<div class="p_font_size"><small>2.What's the argument about Genetically modified product safety?</small></div>
 +
<div class="p_font_size"><small>3.How to evaluate the safety of genetically modified products</small></div>
 +
<div class="p_font_size"><small>4.Can we detect genetically modified ingredients</small></div>
 +
<div class="p_font_size"><small>5.What are genetically modified organisms test method</small></div>
 +
<div class="h4_font_size"><font color="navy"><small>2.Genetically modified regulatory policy</small></font></div>
 +
<div class="p_font_size"><small>1. How to regulate genetically modified (gm) technology and its products</small></div>
 +
<br>
 +
<div class="h4_font_size" align="center"><font color="navy">the chapter of SCAU-CHINA team</font></div>
 +
<div class="h4_font_size"><font color="navy"><small>1.SCAU-CHINA team</small></font></div>
 +
<div class="p_font_size"><small>1.What is iGEM?</small></div>
 +
<div class="p_font_size"><small>2.Who is SCAU-CHINA team?</small></div>
 +
<div class="p_font_size"><small>3.What is the project of SCAU-CHINA?</small></div>
 +
<div class="h4_font_size"><font color="navy"><small>2.SCAU-CHINA team’s Project results——Astaxanthin rice</small></font></div>
 +
<div class="p_font_size"><small>1.the introduction of Technical principle</small></div>
 +
<div class="p_font_size"><small>2.Technology application</small></div>
 +
</div>
 +
<div class="h3_font_size"><a href="" text-decoration=underline>If you want to read more about our handbook, you can download it here!</a></div>
 +
<br>
 +
<div class="h3_font_size">2.2 Bauhinia Festival on Science and Culture</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/8/8e/T--SCAU-China--Gold13.png">
 +
</div>
 +
</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/2/27/T--SCAU-China--Gold14.png">
 +
</div>
 +
</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s2"></div>
 +
<div class="col s8" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/5/5b/T--SCAU-China--Gold15.png">
 +
</div>
 +
</div>
 +
<div class="p_font_size">In South China Agricultural University(SCAU),Bauhinia Festival on Science,Technology and Culture is a great activity every year On this day,tens of thousands of citizens in Guangzhou gather together in SCAU to enjoy the sight of bauhinia and new research in our university. This year,we seized this opportunity and showed interesting biology to hundreds of visitors,mostly young persons and childs.We showed fluorescent protein,microscope and tissue culture technology.These attracted a lot of citizens.In the meantime,we brought some books and brochures about some laws and funny things about GMO.For example,sweet potato has some DNA parts which are originally came from Agrobacterium,which means that transgenic is exist in the natural world.</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s6" align="center">
 +
<img alt="image" class="img-responsive col s11" src="https://static.igem.org/mediawiki/2016/d/dd/T--SCAU-China--Engagement19.png">
 +
</div>
 +
<div class="col s6" align="center">
 +
<img alt="image" class="img-responsive col s11" src="https://static.igem.org/mediawiki/2016/c/cb/T--SCAU-China--Engagement20.png">
 +
</div>
 +
</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/b/b7/T--SCAU-China--Gold18.png">
 +
</div>
 +
</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/2/21/T--SCAU-China--Gold19.png">
 +
</div>
 +
</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" width="800px" src="https://static.igem.org/mediawiki/2016/0/01/T--SCAU-China--Gold20.png">
 +
</div>
 +
</div>
 +
<div class="p_font_size">To attract more people to visit our stall,we also made some beautiful postcards about SCAU or iGEM.</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/c/c5/T--SCAU-China--Engagement22.png">
 +
</div>
 +
</div>
 +
<div class="p_font_size">Our work finally turned out to be a great success.The most famous newspaper in our city--Guangzhou Daily interviewed our team leader and reported us with a brief introduction of our project and activity.SCAU newspaper and SCAU Official WeChat also reported us.</div>
 +
<div class="p_font_size">We even invited visitors to taste our genetically modified papayas.All the papayas selling in markets are GMO,but they’ve been commercialized for many years.Through this activity,we want to convey a message that GM crops are not devils,if we utilize transgenic technology in a reasonable way,they can be soft and sweet like papaya!</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s2"></div>
 +
<div class="col s8" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/a/a7/T--SCAU-China--Engagement24.png">
 +
</div>
 +
</div>
 +
<br>
 +
<div class="h3_font_size">2.3 Education</div>
 +
<div class="p_font_size">Our education including two parts:"Spreading scientific knowledge to countrysides"and"Giving courses for high school students".</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/d/d6/T--SCAU-China--Engagement29.png">
 +
</div>
 +
</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/a/ab/T--SCAU-China--Engagement30.png">
 +
</div>
 +
</div>
 +
<br>
 +
<div class="h3_font_size">2.4 Spread scientific knowledge to countrysides</div>
 +
<div class="p_font_size">Social justice is a important question we are concerning about.As we all known,our country is a developing country and those people in countrysides can hardly get as much information and knowledge as those in cities.So after we did the education work in several high schools in Guangzhou,we decided to do more efforts to spread our knowledge to farmers and students in countrysides.We had sent our educational handbooks to farmers and schools from countrysides in Qingyuan,Shaoguan,Maoming and Zhaoqing,both are locating in Guangdong province.</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s1"></div>
 +
<div class="col s10" align="center">
 +
<img alt="image" class="img-responsive" width="800px" src="https://static.igem.org/mediawiki/2016/7/7b/T--SCAU-China--Intergrated39.jpg">
 +
</div>
 +
</div>
 +
<div class="p_font_size">The first thing we’ve done is sending handbooks about modern agriculture to farmers in these remote places.The handbooks contains a lot information about modern methods of cultivating the local specialty crops,including protocol of cultivating watermelon and pitaya fruit in modern way .It's possible that we can help some people in countryside who is eager to get more information on agriculture,and they might generally accept modern agriculture or even genetically modified crops.</div>
 +
<div class="p_font_size">Now that we need to use our knowledge to help people in countrysides,we think we'd better offer lessons for next generation,so our teammates joined in teams which are going to give courses in primary schools in countrysides and mainly worked in sending basic and funny biology lessons.Those kids are very fond of us and maybe our lessons will actually influence their life and utilize the new knowledge to change their fate.</div>
 +
<br>
 +
<div class="h3_font_size">2.5 Giving courses for high school students</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s2"></div>
 +
<div class="col s8" align="center">
 +
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/4/49/T--SCAU-China--Engagement31.png">
 +
</div>
 +
</div>
 +
<div class="p_font_size">We also gave courses for high school students from Guangzhou NO.47 Middle School,Guangdong experimental High School and Guangdong Guangya High School.Our course mainly focus on the advance research in biological area in these years,especially synthentic biology.We've also introduced iGEM to them and showed them how to take part in iGEM as a high school student.After that,we also built up a good relationship with a high school group from Guangdong experimental High School and Guangdong Guangya High School.</div>
 +
<div class="p_font_size">According to the feedback of our students,they have a better understanding of biology after our lessons.It's quite a good experience to communicate with middle school students and teach them what we've obtained in taking iGEM competition and doing biological research.</div>
 +
<br>
 +
<div class="h3_font_size">2.6 Exhibitions at university's Hacker-space</div>
 +
<div class="row" style="margin-top:20px">
 +
<div class="col s6" align="center">
 +
<img alt="image" class="img-responsivecol s11" src="https://static.igem.org/mediawiki/2016/d/d6/T--SCAU-China--Engagement32.png">
 +
</div>
 +
<div class="col s6" align="center">
 +
<img alt="image" class="img-responsive col s11" src="https://static.igem.org/mediawiki/2016/b/b6/T--SCAU-China--Engagement33.png">
 +
</div>
 +
</div>
 +
<div class="p_font_size">According to investigation,it seems that people are not very willing to use astaxanthin.So we participated in several exhibitions at university’s heckerspace and showed our posters about astaxanthin and astarice.These exhibitions have attracted the attention of the whole society,even some officials from native government attended.</div>
 +
</div>  
 +
</div>  
 +
 
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Revision as of 20:35, 14 October 2016

SCAU

Gold
Our human practice work involved several questions relating to bio-safety,social justice and public acceptance.Our astarice is actually a genetically modified organism,so our work focused on people’s concern about GMO.
1. Investigation
image
We hoped our human practice work can influence more ordinary people.So,above all,we should realize other people's opinion to transgenic food.Because of that,we made a questionnaire about GMO.
The data we’ve obtained in questionnaire is showing below:
The respondents’ proportion of men and women is approximately balanced. The number of age group which between 18 from 25 is the majority. What’s more , the respondents have a Bachelor degree or a higher degree.
image
1.1 The main content

Question 4: Do you know what is Genetically modified (gm)?
image
Analysis of the Q4:4/5 of the respondents have a certain understanding for "GM technology",1/5 respondents don’t understand this technology or even don’t know. This suggests that the "GM technology" was received by the majority of respondents.

Question 6: Will you eat gm food?
image
image
Analysis :About a third of the people can accept genetically modified (gm) food. 17% of people determined not to eat genetically modified food.Suggest that the public acceptance for genetically modified foods is improving.

Question 11: Is transgenosis beneficial or harmful in your opinions?
image
image
image
Analysis : Most people had skeptical attitude to genetically modification (gm) . For respondents,genetically modification is an emerging technology. The public don’t understand enough about genetically modified crops,so they cannot hold a certain attitude to it.

Question 12: Will you use gm products (non-food)?
image

Question 13: Have you ever heard of skin care product that contains astaxanthin?
image
image

Question 14:Will you choose skin care product or health food contains astaxanthin (gm) ?
image
If new genetically modified foods were put into market, about half of people can fully accept.While the acceptance of gm products (non-food) is even higher . However,our public do not know much about astaxanthin product at present, So we should Enlarge the propaganda.
According to the data we’ve got from investigation,we can see that most people are holding a neutral opinion on development of GMO,so it’s possible that we can help them to learn more about transgenic and dispel their qualm,thus make more people accept GMO products.So as to reach this aim,we designed a series of interesting activities.
2. Spreading knowledge to our society
2.1 Writing GMO handbook
After participating in several lectures and talks,we learnt a lot.Later we wrote a handbook about GMO as a summary,which contains our answers to many questions about GMO.We can use this as educational material.The list below is content of our handbook.

the chapter of popular science
1.What are you eat
1. Brief introduction to genetically modified food that you eat
2. A reasonable concern——glyphosate
3. Better gm products
2.Pathological obsession——Opposition to genetically modified
1.‘They’ want to confuse you
2. The preliminary victory——Papaya
3. Organic food is not more secure
4. Being hated humanitarian projects——Golden rice

the chapter of Genetically modified knowledge
1.Basic knowledge of genetically modified
1.What is genetically modified
2.What's the relationship between genetic and biological traits
3.What is a genetically modified technology and products
2.The characteristics of the genetically modified technology
1.What is the commonly used genetically modified (gm) technology
2.What similarities and differences between transgenic technology and the traditional breeding
3.What are genetically modified (gm) technology applications
3.The development of genetically modified
1.How is Genetically modified that produce and develop?
2.What are genetically modified research direction in the global
3.What is the matter of the industrialization of the genetically modified organisms
4.Genetically modified technology development present situation

the chapter of Safety regulation
1.Genetically modified product safety
1.what aspects that Genetically modified product safety involves
2.What's the argument about Genetically modified product safety?
3.How to evaluate the safety of genetically modified products
4.Can we detect genetically modified ingredients
5.What are genetically modified organisms test method
2.Genetically modified regulatory policy
1. How to regulate genetically modified (gm) technology and its products

the chapter of SCAU-CHINA team
1.SCAU-CHINA team
1.What is iGEM?
2.Who is SCAU-CHINA team?
3.What is the project of SCAU-CHINA?
2.SCAU-CHINA team’s Project results——Astaxanthin rice
1.the introduction of Technical principle
2.Technology application

2.2 Bauhinia Festival on Science and Culture
image
image
image
In South China Agricultural University(SCAU),Bauhinia Festival on Science,Technology and Culture is a great activity every year On this day,tens of thousands of citizens in Guangzhou gather together in SCAU to enjoy the sight of bauhinia and new research in our university. This year,we seized this opportunity and showed interesting biology to hundreds of visitors,mostly young persons and childs.We showed fluorescent protein,microscope and tissue culture technology.These attracted a lot of citizens.In the meantime,we brought some books and brochures about some laws and funny things about GMO.For example,sweet potato has some DNA parts which are originally came from Agrobacterium,which means that transgenic is exist in the natural world.
image
image
image
image
image
To attract more people to visit our stall,we also made some beautiful postcards about SCAU or iGEM.
image
Our work finally turned out to be a great success.The most famous newspaper in our city--Guangzhou Daily interviewed our team leader and reported us with a brief introduction of our project and activity.SCAU newspaper and SCAU Official WeChat also reported us.
We even invited visitors to taste our genetically modified papayas.All the papayas selling in markets are GMO,but they’ve been commercialized for many years.Through this activity,we want to convey a message that GM crops are not devils,if we utilize transgenic technology in a reasonable way,they can be soft and sweet like papaya!
image

2.3 Education
Our education including two parts:"Spreading scientific knowledge to countrysides"and"Giving courses for high school students".
image
image

2.4 Spread scientific knowledge to countrysides
Social justice is a important question we are concerning about.As we all known,our country is a developing country and those people in countrysides can hardly get as much information and knowledge as those in cities.So after we did the education work in several high schools in Guangzhou,we decided to do more efforts to spread our knowledge to farmers and students in countrysides.We had sent our educational handbooks to farmers and schools from countrysides in Qingyuan,Shaoguan,Maoming and Zhaoqing,both are locating in Guangdong province.
image
The first thing we’ve done is sending handbooks about modern agriculture to farmers in these remote places.The handbooks contains a lot information about modern methods of cultivating the local specialty crops,including protocol of cultivating watermelon and pitaya fruit in modern way .It's possible that we can help some people in countryside who is eager to get more information on agriculture,and they might generally accept modern agriculture or even genetically modified crops.
Now that we need to use our knowledge to help people in countrysides,we think we'd better offer lessons for next generation,so our teammates joined in teams which are going to give courses in primary schools in countrysides and mainly worked in sending basic and funny biology lessons.Those kids are very fond of us and maybe our lessons will actually influence their life and utilize the new knowledge to change their fate.

2.5 Giving courses for high school students
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We also gave courses for high school students from Guangzhou NO.47 Middle School,Guangdong experimental High School and Guangdong Guangya High School.Our course mainly focus on the advance research in biological area in these years,especially synthentic biology.We've also introduced iGEM to them and showed them how to take part in iGEM as a high school student.After that,we also built up a good relationship with a high school group from Guangdong experimental High School and Guangdong Guangya High School.
According to the feedback of our students,they have a better understanding of biology after our lessons.It's quite a good experience to communicate with middle school students and teach them what we've obtained in taking iGEM competition and doing biological research.

2.6 Exhibitions at university's Hacker-space
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According to investigation,it seems that people are not very willing to use astaxanthin.So we participated in several exhibitions at university’s heckerspace and showed our posters about astaxanthin and astarice.These exhibitions have attracted the attention of the whole society,even some officials from native government attended.