Difference between revisions of "Team:Wageningen UR/Parts"

Line 24: Line 24:
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K1913000">BBa_K1913000</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K1913000">BBa_K1913000</a></td>
−
     <td>ChiA for Varroa destructor</td>
+
     <td>chiA for Varroa destructor</td>
 
     <td>Lisa</td>
 
     <td>Lisa</td>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
 
     <td><a href="http://parts.igem.org/Part:BBa_K1913001">BBa_K1913001</a></td>
 
     <td><a href="http://parts.igem.org/Part:BBa_K1913001">BBa_K1913001</a></td>
−
     <td>ChiB for Varroa destructor</td>
+
     <td>chiB for Varroa destructor</td>
 
     <td>Lisa</td>
 
     <td>Lisa</td>
 
   </tr>
 
   </tr>
Line 43: Line 43:
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
     <td><a href="http://parts.igem.org/Part:BBa_K1913002">BBa_K1913002</a></td>
+
     <td><a href="http://parts.igem.org/Part:BBa_K1913005">BBa_K1913005</a></td>
−
     <td>chiA device regulated by pBAD</td>
+
     <td>lux quorum sensing system + GFP reporter</td>
−
     <td>Lisa</td>
+
     <td>Thomas</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K1913006">BBa_K1913006</a></td>
 +
    <td>434- and lambda cI balance operon + mRFP reporter</td>
 +
    <td>Thomas</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K1913007">BBa_K1913007</a></td>
 +
    <td>434- and lambda cI operon for tuning protein balance</td>
 +
    <td>Thomas</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K19130014">BBa_K19130014</a></td>
 +
    <td>3-oxo-hexanoyl-HSL GFP reporter</td>
 +
    <td>Thomas</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K19130016">BBa_K19130016</a></td>
 +
    <td>434- and lambda cI balance RFP reporter</td>
 +
    <td>Thomas</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K1913008">BBa_K1913008</a></td>
 +
    <td>vitamin b12 riboswitch</td>
 +
    <td>Carina</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K1913009">BBa_K1913009</a></td>
 +
    <td>Guanine riboswitch</td>
 +
    <td>Carina</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K19130010">BBa_K19130010</a></td>
 +
    <td>tetR QPI + mRFP</td>
 +
    <td>Carina</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K19130011">BBa_K19130011</a></td>
 +
    <td>Vitamin b12 riboswitch + mRFP</td>
 +
    <td>Carina</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K19130012">BBa_K19130012</a></td>
 +
    <td>Guanine riboswitch + guanine riboswitch</td>
 +
    <td>Carina</td>
 
   </tr>
 
   </tr>
 +
 +
 
</table>
 
</table>
  

Revision as of 16:24, 15 October 2016

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Wageningen UR iGEM 2016

 

BioBrick number BioBrick name Designer
BBa_K1913000 chiA for Varroa destructor Lisa
BBa_K1913001 chiB for Varroa destructor Lisa
BBa_K1913002 chiA device regulated by pBAD Lisa
BBa_K1913003 chiB device regulated by pBAD Lisa
BBa_K1913005 lux quorum sensing system + GFP reporter Thomas
BBa_K1913006 434- and lambda cI balance operon + mRFP reporter Thomas
BBa_K1913007 434- and lambda cI operon for tuning protein balance Thomas
BBa_K19130014 3-oxo-hexanoyl-HSL GFP reporter Thomas
BBa_K19130016 434- and lambda cI balance RFP reporter Thomas
BBa_K1913008 vitamin b12 riboswitch Carina
BBa_K1913009 Guanine riboswitch Carina
BBa_K19130010 tetR QPI + mRFP Carina
BBa_K19130011 Vitamin b12 riboswitch + mRFP Carina
BBa_K19130012 Guanine riboswitch + guanine riboswitch Carina

Besides, we submitted one part that cannot be classified as a biobrick:

BBa_K1913015 Cry3Aa with araC/pBAD Jaccoline/Linea

When we started making the constructs for the Cas9 kill switch, two approaches were taken: one was taking the Cas9 that is available in the iGEM registry (BBa_K1218011) as a starting point for making mutations and expressing Cas9. The other approach was starting with pdCas9 (Addgene plasmid # 46569). While cloning, it proved to be difficult to transfer BBa_K1218011 to another backbone (see also the notebook). Furthermore, when cultures transformed with BBa_K1218011 were checked for Cas9 expression with SDS-PAGE, no convincing Cas9 band could be observed. Because of time limitations we decided to continue working with the Addgene construct for making the mutations, and chose an established system for protein expression. For that reason, no Cas9-biobricks were submitted. Furthermore, the pEVOL construct containing an aminoacyl-synthetase and a tRNA for introducing BipA in response to the TAG stopcodon were isolated from a strain kindly received from George Church (described in Mandel et al., 2015). The MTA that was signed to receive the strain does not allow for redistribution.

BioBrick 1

YOUR TEXT HERE
Link to favourite biobrick should link to Wageningen_UR/Basic_Part
Link to set of favourite biobricks should link to Wageningen_UR/Part_Collection

BioBrick 2

BioBrick 3