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This construct is a control to test the metabolic load of the Hsp104-CFP fusion protein and as a baseline against which we compare the change in [PSI+]/[psi-] state. We synthesized a fragment to clone into the [Hsp-PRS315]. This plasmid will be referred to as the Hsp104 plasmid and is used in several of our experiments. | This construct is a control to test the metabolic load of the Hsp104-CFP fusion protein and as a baseline against which we compare the change in [PSI+]/[psi-] state. We synthesized a fragment to clone into the [Hsp-PRS315]. This plasmid will be referred to as the Hsp104 plasmid and is used in several of our experiments. | ||
+ | <img src="https://static.igem.org/mediawiki/2016/e/e6/T--Waterloo--HSP104_LEU_Addgene_Annotate.png"> | ||
+ | <div class="wcontent-caption"> | ||
+ | The Hsp104 plasmid. | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> |
Revision as of 19:46, 15 October 2016
Hsp104-NSC
This construct is a control to test the metabolic load of the Hsp104-CFP fusion protein and as a baseline against which we compare the change in [PSI+]/[psi-] state. We synthesized a fragment to clone into the [Hsp-PRS315]. This plasmid will be referred to as the Hsp104 plasmid and is used in several of our experiments.