Difference between revisions of "Team:Waterloo/Experiments"
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| − | <li> <a href="" ng-click="active='digest'"> Restriction Enzyme Digest </a></li> | + | |
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| + | <li> <a href="" ng-click="active='digest'"> Restriction Enzyme Digest </a></li> | ||
<li><a href="" ng-click="active='inoculation'">Inoculating Liquid Culture</a></li> | <li><a href="" ng-click="active='inoculation'">Inoculating Liquid Culture</a></li> | ||
<li><a href="" ng-click="active='miniprep'">Plasmid Miniprep</a></li> | <li><a href="" ng-click="active='miniprep'">Plasmid Miniprep</a></li> | ||
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<li><a href="" ng-click="active='PCR'"> PCR Amplification </a></li> | <li><a href="" ng-click="active='PCR'"> PCR Amplification </a></li> | ||
<li><a href="" ng-click="active='cPCR'"> Colony PCR </a></li> | <li><a href="" ng-click="active='cPCR'"> Colony PCR </a></li> | ||
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Revision as of 20:00, 15 October 2016
Escherichia coli
Saccharomyces cerevisae
Fluorescence
Inoculating Liquid Culture
- Asceptically pipette 5mL sterile liquid broth into a test tube. If there are no large pipette tips able to transfer 5mL of liquid broth, use a fresh falcon tube to measure.
- Add appropriate antibiotic, if required, to desired concentration using a pipette. You will have to tilt the test tube so that the broth is close enough to the top of the tube that you can add the antibiotic to the broth. Dropping it in won’t work because you will typically be adding ~2.5mL and the drop will be too small to fall.
- It is recommended that you make a “mastermix” with broth and antibiotics in it when you have many tubes that require the same antibiotic.
