Difference between revisions of "Team:Waterloo/Experiments"
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<i>Escherichia coli</i> | <i>Escherichia coli</i> | ||
</div> | </div> | ||
| − | <div class=" | + | <div class="wcontent_alt"> |
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| − | + | <a href="" ng-click="active='digest'"> Restriction Enzyme Digest </a> | |
| − | < | + | </div> |
| − | < | + | <div class="wcontent_alt"> |
| − | < | + | <a href="" ng-click="active='inoculation'">Inoculating Liquid Culture</a> |
| − | < | + | </div> |
| + | <div class="wcontent_alt"> | ||
| + | <a href="" ng-click="active='miniprep'">Plasmid Miniprep</a> | ||
| + | </div> | ||
| + | <div class="wcontent_alt"> | ||
| + | <a href="" ng-click="active='gel'"> Agarose Gel</a> | ||
| + | </div> | ||
| + | <div class="wcontent_alt"> | ||
| + | <a href="" ng-click="active='electro'"> Agarose Gel Electrophoresis</a> | ||
| + | </div> | ||
<li><a href="" ng-click="active='extraction'"> Gel Extraction </a></li> | <li><a href="" ng-click="active='extraction'"> Gel Extraction </a></li> | ||
<li><a href="" ng-click="active='purify'"> Column Purification </a></li> | <li><a href="" ng-click="active='purify'"> Column Purification </a></li> | ||
Revision as of 20:03, 15 October 2016
Saccharomyces cerevisae
Fluorescence
Inoculating Liquid Culture
- Asceptically pipette 5mL sterile liquid broth into a test tube. If there are no large pipette tips able to transfer 5mL of liquid broth, use a fresh falcon tube to measure.
- Add appropriate antibiotic, if required, to desired concentration using a pipette. You will have to tilt the test tube so that the broth is close enough to the top of the tube that you can add the antibiotic to the broth. Dropping it in won’t work because you will typically be adding ~2.5mL and the drop will be too small to fall.
- It is recommended that you make a “mastermix” with broth and antibiotics in it when you have many tubes that require the same antibiotic.
