Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-pGFP C120 RFP"

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style="color: #666666 !important; text-shadow: 0px 0px 1px #000000 !important;  /*margin: 12px !important;*/  font-size: 18px !important;  text-decoration: none;">[Expand all]</a></p></center></div>
  
−
<div class="technology">8/5</div>
+
<div class="technology">9/23</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px; list-style-type: disc;">pBAR_PgpdA transformed into competent cell</p></li>
+
<li><p style="font-size: 18px;">GFP_TtrpC ligation PCR</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 0); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/6</div>
+
<div class="technology">9/24</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA containing cultures used in 2 in 1</p></li>
+
<li><p style="font-size: 18px;">GFP_TtrpC ligation PCR</p></li>
 +
<li><p style="font-size: 18px;">GFP_TtrpC ligation PCR (We confirmed junction primer was wrong)</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 1); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/7</div>
+
<div class="technology">9/30</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA extracted and checked with plasmid PCR and restriction digestion</p></li>
+
<li><p style="font-size: 18px;">Received the junction primer of GFP-TtrpC</p></li>
 +
<li><p style="font-size: 18px;">GFP_TtrpC ran ligation PCR</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/14</div>
+
<div class="technology">10/1</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA plasmid stored and transformed cultures cryopreserved</p></li>
+
<li><p style="font-size: 18px;">GFP_TtrpC ran ligation PCR</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 3); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/18</div>
+
<div class="technology">10/2</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA digested for ligation with pVP-EL222 and TtrpC</p></li>
+
<li><p style="font-size: 18px;">GFP_TtrpC digested for ligation with pBARGPE1</p></li>
 +
<li><p style="font-size: 18px;">Transformed pBAR_GFP_TtrpC into competent cells</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 4); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/19</div>
+
<div class="technology">10/3</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC containing cultures used in 2 in 1</p></li>
 +
<li><p style="font-size: 18px;">pExtracted plasmid pBAR_GFP_TtrpC</p></li>
 +
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC digestion check(failure)</p></li>
 +
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC PCR check(failure)</p></li>
 +
<li><p style="font-size: 18px;">Transformed pBAR_GFP_TtrpC into competent cells</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">[Collapse]</a></p>
 
</div>
 
</div>
  
−
<div class="technology">8/21</div>
+
<div class="technology">10/4</div>
 
<div class="thelanguage">
 
<div class="thelanguage">
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC extracted and checked with restriction digestion (failure)</p></li>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC containing cultures used in 2 in 1</p></li>
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
+
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC plasmid extraction</p></li>
 +
<li><p style="font-size: 18px;">pBAR_GFP_TtrpC digestion check</p></li>
  
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
 
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 6); return false">[Collapse]</a></p>
−
</div>
 
−
 
−
<div class="technology">8/23</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 7); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">8/24</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 8); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">8/28</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR ------- NC had band</p></li>
 
−
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR ------ with remixed primer</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 9); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">8/29</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR ------ the concentration was not high enough after gel extraction</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 10); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">8/31</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 11); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/1</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">EL222 and TtrpC ran ligation PCR</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 12); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/2</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">EL222-TtrpC checked with restriction digestion</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA digested for ligation with EL222_TtrpC</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 13); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/3</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA digested for ligation with EL222_Ttrp</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 14); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/4</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC extracted from transformed cultures</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 15); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/5</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 16); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/6</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (failure)</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (we changed  enzymes)</p></li>
 
−
<li><p style="font-size: 18px;">EL222-TtrpC digested with restriction enzyme</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 17); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/9</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell( there was no E.coli on our first plate )</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 18); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/10</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC transformed into competent cell</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 19); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/11</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC containing culture used in 3 in 1</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 20); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/12</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC checked with restriction digestion</p></li>
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC amplified with plasmid PCR</p></li>
 
−
<li><p style="font-size: 18px;">Successfully constructed the plasmid pBAR_PgpdA_EL222_TtrpC</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 21); return false">[Collapse]</a></p>
 
−
</div>
 
−
 
−
<div class="technology">9/22</div>
 
−
<div class="thelanguage">
 
−
<li><p style="font-size: 18px;">pBAR_PgpdA_EL222_TtrpC digested for transforming into Metarhizium anisopliae</p></li>
 
−
 
−
<p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 22); return false">[Collapse]</a></p>
 
 
</div>
 
</div>
  

Revision as of 22:47, 15 October 2016

pGFP_C120_RFP

pBARGPE1

GFP

TtrpC

C120_empty

minimal-PgpdA

RFP

pGFP_C120_RFP


[Collapse all] | [Expand all]

9/23

  • GFP_TtrpC ligation PCR

    • [Collapse]

    9/24

  • GFP_TtrpC ligation PCR

  • GFP_TtrpC ligation PCR (We confirmed junction primer was wrong)

    • [Collapse]

    9/30

  • Received the junction primer of GFP-TtrpC

  • GFP_TtrpC ran ligation PCR

    • [Collapse]

    10/1

  • GFP_TtrpC ran ligation PCR

    • [Collapse]

    10/2

  • GFP_TtrpC digested for ligation with pBARGPE1

  • Transformed pBAR_GFP_TtrpC into competent cells

    • [Collapse]

    10/3

  • pBAR_GFP_TtrpC containing cultures used in 2 in 1

  • pExtracted plasmid pBAR_GFP_TtrpC

  • pBAR_GFP_TtrpC digestion check(failure)

  • pBAR_GFP_TtrpC PCR check(failure)

  • Transformed pBAR_GFP_TtrpC into competent cells

    • [Collapse]

    10/4

  • pBAR_GFP_TtrpC containing cultures used in 2 in 1

  • pBAR_GFP_TtrpC plasmid extraction

  • pBAR_GFP_TtrpC digestion check

    • [Collapse]