Revision as of 13:56, 17 October 2016

Team:HokkaidoU Japan - 2016.igem.org

"

Team:HokkaidoU Japan

\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\

notebook_2016

2016/08/07

Transformation

Researcher: Fujita

Part: BBa_B0034 on pSB1A2, BBa_E0040 on pSB1A2 BBa_R0010 on pSB1C3, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3, BBa_E0020 on pSB1C3, BBa_E0030 on pSB1C3

Liquid Culture

Researcher: Fujita, Kishida, Kono

Part: BBa_E0020 on 1c3, BBa_E0030 on 1c3, BBa_E0040 on 1a2, BBa_R0010 on 1c3

2016/08/09

Transformation

Researcher: Kono, Mimata, Nishimura

Part: BBa_B0034 on pSB1A2, BBa_E0040 on pSB1A2, BBa_R0010 on pSB1C3, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3

Mini-prep

Researcher: Fujita, Kishida, Kono

Part: BBa_R0010 on pSB1A2, BBa_E0040 on pSB1A2

Gel Free System

Researcher: Mimata, Shibata, Kono, Nishimura

Part: BBa_R0010, BBa_E0040

Ethanol Precipitation

Researcher: Mimata, Shibata, Kono, Nishimura

Part: BBa_R0010, BBa_E0040

2016/08/10

Liquid Culture

Researcher: Mimata

Part: BBa_B0015 on 1c3, BBa_J04500 on 1c3, BBa_R0010 on 1c3

Transformation

Researcher: Mimata

Part: BBa_B0034 on pSB1A2, BBa_E0040 on pSB1A2

Electrophoresis

Researcher: Kishimoto, Kono, Shibata, Nishimura

Part: BBa_R0010, BBa_E0040

Mini-prep

Researcher: Mimata, Kono

Part: BBa_R0010 on pSB1A2, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3

Electrophoresis

Researcher: Mimata, Kono, Nishimura

Part: BBa_R0010 on pSB1A2, BBa_B0015 on pSB1C3, BBa_J04500 on pSB1C3

Liquid Culture

Researcher: Kono, Mimata, Nishimura

Part: BBa_B0034 on 1a2, BBa_E0040 on 1c3

2016/08/11

Transformation

Researcher: Nishimura, Kishida

Part: BBa_B0032 on pSB1C3, BBa_R0011 on pSB1A2

Mini-prep

Researcher: Nishimura, Kishida

Part: BBa_E0040 on pSB1A2, BBa_B0034 on pSB1A2

PEG Precipitation

Researcher: Nishimura, Shibata, Noda

Part: BBa_B0015 on pSB1C3, BBa_R0010 on pSB1C3 , BBa_J04500 on pSB1C3, BBa_E0040 on pSB1A2, BBa_B0034 on pSB1A2

Electrophoresis

Researcher: Shibata, Noda

Part: BBa_B0015 on pSB1C3, BBa_R0010 on pSB1C3 , BBa_J04500 on pSB1C3, BBa_E0040 on pSB1A2, BBa_B0034 on pSB1A2

Gel Free System

Researcher: Kono, Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

Electrophoresis

Researcher: Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

Ethanol Precipitation

Researcher: Kono, Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

Electrophoresis

Researcher: Kono, Nishimura

Part: BBa_B0015, BBa_R0010, BBa_J04500, BBa_E0040, BBa_B0034

2016/08/12

Transformation

Researcher: Nishimura

Part: BBa_C0012 on pSB1C3, BBa_J23101 on BBa_J61002

Liquid Culture

Researcher: Nishimura

Part: BBa_B0032 on 1c3, BBa_R0011 on 1a2

2016/08/13

Mini-prep

Researcher: Fujita

Part: BBa_B0032 on pSB1C3

PEG Precipitation

Researcher: Fujita

Part: BBa_B0032 on pSB1C3

Electrophoresis

Researcher: Fujita, Noda

Part: BBa_B0032 on pSB1C3

Streaking (Single Colony Isolation)

Researcher: Shibata, Fujita

Part: BBa_R0011 on pSB1A2

Liquid Culture

Researcher: Shibata, Noda

Part: BBa_C0012 on 1c3, BBa_B0032 on 1c3, BBa_J23101 on BBa_J61002

2016/08/14

Mini-prep

Researcher: Nishimura, Kishimoto

Part: BBa_C0012 on pSB1C3, BBa_B0032 on pSB1C3, BBa_J23101 on BBa_J61002

PEG Precipitation

Researcher: Nishimura, Kono

Part: BBa_C0012 on pSB1C3, BBa_B0032 on pSB1C3, BBa_J23101 on BBa_J61002

Electrophoresis

Researcher: Nishimura, Kono

Part: BBa_C0012 on pSB1C3, BBa_B0032 on pSB1C3, BBa_J23101 on BBa_J61002

Transformation

Researcher: Nishimura, Kono, Donen

Part: BBa_R0011 on pSB1A2

2016/08/15

Gel Free System

Researcher: Fujita, Esashi

Part: BBa_C0012, BBa_J23101, BBa_B0032

Electrophoresis

Researcher: Kishida, Fujita, Donen, Shibata, Esashi

Part: BBa_C0012, BBa_J23101, BBa_B0032

Ethanol Precipitation

Researcher: Fujita, Kishida, Noda

Part: BBa_C0012, BBa_J23101, BBa_B0032

Liquid Culture

Researcher: Shibata

Part: BBa_R0011 on 1a2

2016/08/16

Electrophoresis

Researcher: Nishimura

Part: BBa_C0012, BBa_J23101, BBa_B0032

Mini-prep

Researcher: Nishimura

Part: BBa_R0011 on pSB1A2

PEG Precipitation

Researcher: Nishimura, Kono

Part: BBa_R0011 on pSB1A2

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011 on pSB1A2

PCR (2 STEP)

Researcher: Esashi, Nishimura, Shibata

Part: BBa_R0011

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011

2016/08/17

Gel Free System

Researcher: Sugishita, Shibata

Part: BBa_R0011

Electrophoresis

Researcher: Sugishita, Shibata, Kishida

Part: BBa_R0011

Gel Free System

Researcher: Sugishita, Kishida, Shibata

Part: BBa_R0011

PEG Precipitation

Researcher: Sugishita, Kishida, Shibata

Part: BBa_R0011

Transformation

Researcher: Nishimura

Part: BBa_I13504 on pSB1C3

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011

Transformation

Researcher: Shibata

Part: BBa_I13504 on pSB1C3

2016/08/18

Gel Free System

Researcher: Nishimura

Part: BBa_R0011

Ethanol Precipitation

Researcher:

Part: BBa_R0011

Electrophoresis

Researcher: Nishimura

Part: BBa_R0011

Liquid Culture

Researcher: Nishimura

Part: BBa_I13504 on 1c3

Transformation

Researcher: Kono

Part: BBa_I13504 on pSB1C3

2016/08/19

Mini-prep

Researcher: Shibata, Kishida

Part: BBa_I13504 on pSB1C3

PEG Precipitation

Researcher: Shibata

Part: BBa_I13504 on pSB1C3

Electrophoresis

Researcher: Shibata

Part: BBa_I13504 on pSB1C3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_I13504

Electrophoresis

Researcher: Shibata, Nishimura

Part: BBa_I13504

PCR (2 STEP)

Researcher: Nishimura, Shibata

Part: BBa_I13504

Electrophoresis

Researcher: Shibata, Nishimura

Part: BBa_I13504

2016/08/20

Gel Free System

Researcher: Fujita, Mimata

Part: BBa_I13504

Ethanol Precipitation

Researcher: Fujita, Mimata

Part: BBa_I13504

2016/08/21

Electrophoresis

Researcher: Nishimura, Mimata, Kishimoto

Part: BBa_I13504

Ligation

Researcher: Kishimoto, Nishimura, Mimata

Part: BBa_I13504 & BBa_R0011 on pSB1A2

Transformation

Researcher: Kishimoto, Kono, Mimata

Part: BBa_I13401 on pSB1A2, BBa_R0011 - BBa_I13504 on pSB1A2

2016/08/22

Colony PCR

Researcher: Mimata, Kono, Kishida, Esashi

Part: BBa_R0011 - BBa_I13504

Liquid Culture

Researcher: Mimata, Kono, Kishida, Esashi

Part: BBa_R0011 - BBa_I13504 on 1a2, BBa_I13401 on 1a2

2016/08/23

Mini-prep

Researcher: Mimata, Fujita

Part: BBa_I13401 on pSB1A2

PEG Precipitation

Researcher: Mimata, Fujita

Part: BBa_I13401 on pSB1A2, BBa_R0011 on pSB1A2

Digestion

Researcher: Mimata, Fujita, Yonezawa

Part: BBa_R0011 on pSB1A2

Gel Free System

Researcher: Mimata, Fujita, Yonezawa

Part: BBa_I13504

Ethanol Precipitation

Researcher: Mimata, Fujita, Yonezawa

Part: BBa_I13504

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 on pSB1A2, BBa_I13504

Ligation

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 on pSB1A2 & BBa_I13504

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 - BBa_I13504 on pSB1A2

Transformation

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 - BBa_I13504 on pSB1A2

Electrophoresis

Researcher: Mimata, Fujita, Kishida, Kono

Part: BBa_R0011 - BBa_I13504 on pSB1A2

2016/08/24

Colony PCR

Researcher: Fujita, Kono

Part: BBa_R0011 - BBa_I13504

Electrophoresis

Researcher: Fujita, Kono, Shibata, Mimata, Hirao

Part: BBa_R0011 - BBa_I13504

PCR (2 STEP)

Researcher: Kono, Shibata, Fujita

Part: IDT1, IDT2, IDT3, IDT4, IDT5, IDT6, IDT7

Electrophoresis

Researcher: Fujita, Kono, Hirao

Part: IDT1, IDT2, IDT3, IDT4, IDT5, IDT6, IDT7

Colony PCR

Researcher: Kono, Hirao

Part: BBa_R0011 - BBa_I13504

Digestion

Researcher: Mimata

Part: IDT2, IDT3

PCR (2 STEP)

Researcher: Fujita

Part: IDT1, IDT5, IDT6, IDT7

Electrophoresis

Researcher: Hirao

Part: BBa_R0011 - I13504

Electrophoresis

Researcher: Mimata

Part: IDT2, IDT3

Electrophoresis

Researcher: Fujita

Part: IDT1, IDT5, IDT6, IDT7

Colony PCR

Researcher: Fujita, Mimata

Part: BBa_R0011 - BBa_I13504

Gel Extract

Researcher: Mimata

Part: IDT2, IDT3

Electrophoresis

Researcher: Mimata

Part: IDT2, IDT3

Digestion

Researcher: Mimata, Fujita

Part: IDT1, pSB1C3

Electrophoresis

Researcher: Mimata, Fujita

Part: pSB1C3, IDT1, IDT2, IDT3

Electrophoresis

Researcher: Mimata, Fujita

Part: BBa_R0011 - I13504

2016/08/25

Digestion

Researcher: Mimata

Part: BBa_J04500, BBa_I13401

Gel Extract

Researcher: Mimata

Part: pSB1C3, IDT1, IDT2, IDT3

Electrophoresis

Researcher: Mimata

Part: BBa_J04500 on pSB1C3, BBa_I13401 on pSB1A2

Electrophoresis

Researcher: Noda, Mimata

Part: pSB1C3, IDT1, IDT2, IDT3

Ethanol Precipitation

Researcher: Mimata, Fujita, Noda

Part: pSB1C3, IDT1, IDT2, IDT3

Electrophoresis

Researcher: Mimata, Fujita

Part: pSB1C3, IDT1, IDT2, IDT3

Electrophoresis

Researcher: Fujita

Part: pSB1C3, IDT1, IDT2, IDT3

Ligation

Researcher: Fujita

Part: pSB1C3 & IDT1

Electrophoresis

Researcher: Fujita

Part: IDT1 on pSB1C3

PCR (2 STEP)

Researcher: Nishimura

Part: IDT5, IDT6

Electrophoresis

Researcher: Nishimura

Part: IDT5, IDT6

Transformation

Researcher: Nishimura, Hirao

Part: IDT1 on pSB1C3

Gel Free System

Researcher: Shibata, Hirao

Part: BBa_I13401

Electrophoresis

Researcher: Nishimura

Part: IDT1, IDT2

PCR (2 STEP)

Researcher: Nishimura

Part: IDT6

Ethanol Precipitation

Researcher: Nishimura, Fujita

Part: IDT1, IDT2, IDT3, IDT4, IDT5, IDT7

Electrophoresis

Researcher: Nishimura

Part: IDT6

Gel Free System

Researcher: Fujita, Noda, Nishimura, Hirao

Part: BBa_I13401

Electrophoresis

Researcher: Hirao

Part: IDT1, IDT2, IDT3, IDT4, IDT5, IDT7

2016/08/26

Colony PCR

Researcher: Nishimura

Part: IDT1

Ethanol Precipitation

Researcher: Hirao, Nishimura, Mimata

Part: BBa_I13401

Electrophoresis

Researcher: Hirao, Nishimura, Mimata

Part: IDT1

Electrophoresis

Researcher: Nishimura

Part: IDT1, IDT2

Digestion

Researcher: Hirao, Mimata, Kono

Part: IDT1

Electrophoresis

Researcher: Mimata, Kono

Part: IDT1

2016/08/27

Liquid Culture

Researcher: Mimata

Part: idt1 on 1c3

PCR (2 STEP)

Researcher: Mimata, Hirao

Part: IDT1, IDT2, IDT3, IDT4, IDT5, IDT6, IDT7

Digestion

Researcher: Hirao, Mimata, Noda

Part: IDT1

Electrophoresis

Researcher: Mimata, Noda, Hirao, Fujita, Shibata

Part: IDT1, IDT2, IDT3, IDT4, IDT5, IDT6, IDT7

Ethanol Precipitation

Researcher: Fujita, Mimata, Shibata

Part: IDT1, IDT2, IDT3, IDT4, IDT5, IDT6, IDT7

Digestion

Researcher: Fujita, Noda, Shibata, Hirao

Part: IDT2, IDT3

Electrophoresis

Researcher: Hirao, Shibata

Part: IDT1, IDT2, IDT3, IDT4, IDT5, IDT6, IDT7

Electrophoresis

Researcher: Hirao, Shibata, Noda

Part: IDT2, IDT3

Ethanol Precipitation

Researcher: Hirao, Shibata

Part: IDT2, IDT3

Electrophoresis

Researcher: Shibata, Noda, Esashi

Part: IDT2, IDT3

Ligation

Researcher: Noda, Esashi, Shibata

Part: IDT2 & IDT3

Electrophoresis

Researcher: Noda, Esashi, Shibata

Part: IDT2 - IDT3

2016/08/28

Mini-prep

Researcher: Mimata

Part: IDT1 on pSB1C3

PEG Precipitation

Researcher: Fujita, Mimata, Hirao

Part: IDT1 on pSB1C3

Digestion

Researcher: Mimata, Hirao

Part: IDT7

PCR (2 STEP)

Researcher: Mimata, Hirao

Part: IDT2 - IDT3

Ethanol Precipitation

Researcher: Mimata, Hirao

Part: IDT7

Electrophoresis

Researcher: Mimata, Nishimura, Hirao

Part: IDT7, IDT1 on pSB1C3

PCR (2 STEP)

Researcher: Mimata, Nishimura, Hirao

Part: IDT6

PCR (3 STEP)

Researcher: Mimata, Nishimura, Hirao

Part: IDT6

PCR (3 STEP)

Researcher: Mimata, Nishimura, Hirao

Part: IDT1

Electrophoresis

Researcher: Mimata, Hirao

Part: IDT2 & IDT3, IDT6

2016/08/29

Electrophoresis

Researcher: Nishimura

Part: idt1 on 1c3

PCR (STEP DOWN)

Researcher: Nishimura

Part: idt6

Colony PCR

Researcher: Nishimura

Part: idt1/span>

Electrophoresis

Researcher: Nishimura

Part: idt6

Electrophoresis

Researcher: Nishimura

Part: idt1

PCR (2 STEP)

Researcher: Mimata, Kishida, Hirao

Part: idt6

Electrophoresis

Researcher: Mimata, Kishida, Hirao

Part: idt1

Electrophoresis

Researcher: Mimata, Kishida, Kono, Hirao

Part: idt6

PCR (2 STEP)

Researcher: Kishida, Kono

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Electrophoresis

Researcher: Kono, Hirao

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Ethanol Precipitation

Researcher: Kishida, Hirao, Kono

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

PCR (2 STEP)

Researcher: Kishida, Noda, Esashi

Part: idt1, idt3, idt5, idt6

Ethanol Precipitation

Researcher: Noda, Esashi

Part: idt6

Electrophoresis

Researcher: Noda, Esashi

Part: idt1, idt3, idt5, idt6

Ethanol Precipitation

Researcher: Kono, Hirao

Part: idt1, idt3, idt5, idt6

2016/08/30

PCR (2 STEP)

Researcher: Mimata

Part: idt6

Digestion

Researcher: Hirao, Kono

Part: idt2, idt3

Ethanol Precipitation

Researcher: Kono, Hirao

Part: idt2

Electrophoresis

Researcher: Mimata

Part: idt6

Electrophoresis

Researcher: Hirao, Kono

Part: idt2, idt3

Ligation

Researcher: Kono, Hirao

Part: idt2 & idt3

Electrophoresis

Researcher: Hirao, Kono, Mimata

Part: idt2 - idt3

Ethanol Precipitation

Researcher: Mimata, Kono

Part: idt2 - idt3

Electrophoresis

Researcher: Nishimura

Part: idt2 - idt3

PCR (2 STEP)

Researcher: Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt7

Electrophoresis

Researcher: Shibata, Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt7

Ethanol Precipitation

Researcher: Kishida, Shibata, Hirao

Part: idt1, idt2, idt3, idt4, idt5, idt7

Electrophoresis

Researcher: Kishida, Hirao

Part: idt1, idt2, idt3, idt4, idt5, idt7

Digestion

Researcher: Hirao, Kishida, Mimata

Part: idt2, idt3

Ethanol Precipitation

Researcher: Kishida, Mimata

Part: idt2

Electrophoresis

Researcher: Mimata, Nishimura

Part: idt2, idt3

Ligation

Researcher: Hirao, Kishida

Part: idt2 & idt3

Ethanol Precipitation

Researcher: Hirao, Mimata

Part: idt2 - idt3

Competent Cells

Researcher: Mimata, Hirao, Noda, Esashi, Kono, Fujita

2016/08/31

Electrophoresis

Researcher: Nishimura

Part: idt2 - idt3

PCR (2 STEP)

Researcher: Nishimura, Hirao

Part: idt2 - idt3

Digestion

Researcher: Kono, Nishimura, Mimata

Part: idt1, idt4, idt5, idt7, psb1c3

Electrophoresis

Researcher: Nishimura, Noda

Part: idt2 - idt3

Ethanol Precipitation

Researcher: Hirao, Mimata, Kishida

Part: idt1, idt4, idt5, idt7

Electrophoresis

Researcher: Nishimura, Noda

Part: idt2 - idt3

Ethanol Precipitation

Researcher: Nishimura, Mimata, Noda, Kono

Part: idt2 - idt3

Electrophoresis

Researcher: Hirao, Kishida

Part: idt1, idt4, idt5, idt6, idt7, psb1c3

Electrophoresis

Researcher: Hirao

Part: idt2 - idt3

Electrophoresis

Researcher: Esashi

Part: idt7 on psb1c3, psb1c3

Gel Extract

Researcher: Hirao, Kishida, Kono

Part: idt2 - idt3

Electrophoresis

Researcher: Hirao, Kono

Part: idt2 - idt3

Transformation

Researcher: Kono

Part: psb1c3

Digestion

Researcher: Esahsi, Mimata

Part: idt1, idt5, psb1c3

PCR (2 STEP)

Researcher: Kono

Part: idt1, idt2, idt3, idt4, idt5, idt7

PCR (2 STEP)

Researcher: Mimata

Part: idt2 - idt3

Ethanol Precipitation

Researcher: Esashi

Part: idt1, idt5, psb1c3

2016/09/01

Electrophoresis

Researcher: Mimata, Esashi, Kono

Part: idt1, idt5, idt2 - idt3, psb1c3

Ethanol Precipitation

Researcher: Kono, Mimata

Part: idt2 - idt3

Ligation

Researcher: Mimata, Kono, Esashi

Part: idt1 & psb1c3, idt5 & psb1c3

Digestion

Researcher: Esashi, Mimata

Part: idt7, psb1c3

Electrophoresis

Researcher: Mimata, Esashi, Kono

Part: idt7, idt1 on psb1c3, idt5 on psb1c3, idt2 - idt3, psb1c3

Ethanol Precipitation

Researcher: Kono, Mimata, Esashi

Part: idt7, psb1c3

Electrophoresis

Researcher: Hirao

Part: idt7, idt2 - idt3, psb1c3

Transformation

Researcher: Nishimura

Part: idt1 on psb1c3, idt5 on psb1c3

Digestion

Researcher: Nishimura, Hirao, Shibata

Part: idt1, idt2, idt3, idt5, idt7

PCR (2 STEP)

Researcher: Noda, Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt7, idt2 - idt3

Electrophoresis

Researcher: Noda

Part: idt1, idt2, idt3, idt4, idt5, idt7

Electrophoresis

Researcher: Hirao, Shibata

Part: idt1, idt2, idt3, idt4, idt5, idt7, idt2 - idt3

Ethanol Precipitation

Researcher: Shibata, Hirao

Part: idt1, idt2, idt3, idt5, idt7

Ethanol Precipitation

Researcher: Shibata, Hirao

Part: idt1, idt2, idt3, idt4, idt5, idt7

PCR (2 STEP)

Researcher: Nishimura, Kishida

Part: rada, p11

Colony PCR

Researcher: Kono

Part: psb1c3

Electrophoresis

Researcher: Noda, Esashi

Part: idt1, idt2, idt3, idt4, idt5, idt7, idt2 - idt3, rada, p11

Ligation

Researcher: Nishimura, Esashi, Noda

Part: idt7 & psb1c3

Liquid Culture

Researcher: Kono

Part: 1c3

PCR (2 STEP)

Researcher: Nishimura, Noda

Part: idt1, idt2, idt3, idt4, idt5, idt7

Electrophoresis

Researcher: Kono

Part: psb1c3

Ethanol Precipitation

Researcher: Noda, Esashi, Nishimura

Part: idt7 & psb1c3

Gel Extract

Researcher: Nishimura, Hirao

Part: idt1, idt2, idt3, idt4, idt5, idt7

Electrophoresis

Researcher: Hirao

Part: idt7 on psb1c3

PCR (3 STEP)

Researcher: Noda

Part: rada, p11

Transformation

Researcher: Nishimura

Part: idt7 on psb1c3

Gel Extract

Researcher: Hirao

Part: idt1, idt2, idt3, idt4, idt5, idt7

2016/09/02

Electrophoresis

Researcher: Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt7

Electrophoresis

Researcher: Noda

Part: rada, p11

Digestion

Researcher: Nishimura

Part: idt2, idt3

PCR Purification

Researcher: Fujita

Part: rada, p11

Electrophoresis

Researcher: Fujita, Nishimura

Part: rada, p11, idt2, idt3

Ethanol Precipitation

Researcher: Nishimura, Fujita

Part: idt2, idt3

Electrophoresis

Researcher: Fujita, Noda

Part: idt2, idt3

Colony PCR

Researcher: Fujita

Part: idt1 on psb1c3, idt5 on psb1c3

Electrophoresis

Researcher: Fujita, Nishimura, Noda

Part: idt1 on psb1c3, idt5 on psb1c3

Ligation

Researcher: Fujita, Noda

Part: idt2 & idt3

Ethanol Precipitation

Researcher: Nishimura, Noda

Part: idt2 - idt3

Mini-prep

Researcher: Fujita, Nishimura

Part: psb1c3

PEG Precipitation

Researcher: Fujita

Part: psb1c3

Ethanol Precipitation

Researcher: Nishimura

Part: idt2 - idt3

Electrophoresis

Researcher: Fujita, Nishimura

Part: psb1c3

Colony PCR

Researcher: Hirao, Fujita

Part: idt1 on psb1c3, idt5 on psb1c3

PCR (2 STEP)

Researcher: Fujita, Hirao

Part: idt2 - idt3

Digestion

Researcher: Fujita, Hirao

Part: psb1c3

Transformation

Researcher: Mimata, Hirao

Part: psb1c3

Electrophoresis

Researcher: Hirao

Part: idt5 on psb1c3

Ethanol Precipitation

Researcher: Mimata

Part: psb1c3

Electrophoresis

Researcher: Hiraa

Part: idt5 on psb1c3

Electrophoresis

Researcher: Mimata

Part: psb1c3, idt2 - idt3

Ethanol Precipitation

Researcher: Mimata, Hirao

Part: idt2 - idt3

Digestion

Researcher: Nishimura

Part: psb1c3

Electrophoresis

Researcher: Nishimura

Part: psb1c3

Colony PCR

Researcher: Nishimura, Hirao

Part: idt7 on psb1c3

Electrophoresis

Researcher: Nishimura

Part: idt7 on psb1c3

Electrophoresis

Researcher: Shibata, Hirap

Part: idt7 on psb1c3

2016/09/03

Electrophoresis

Researcher: Shibata, Hirap, Mimata

Part: idt2 - idt3

Gel Extract

Researcher: Shibata

Part: idt2 - idt3

Electrophoresis

Researcher: Fujita

Part: idt2 - idt3

Liquid Culture

Researcher: Fujita

Part: 1c3

PCR (2 STEP)

Researcher: Fujita, Hirao

Part: idt2 - idt3

Transformation

Researcher: Mimata

Part: psb1c3

Electrophoresis

Researcher: Nishimura, Hirao

Part: idt2 - idt3

2016/09/05

PCR (2 STEP)

Researcher: Hirao

Part: idt2 - idt3

PCR (2 STEP)

Researcher: Hirao, Shibata

Part: idt1, idt2, idt3, idt4, idt5, idt7

Liquid Culture

Researcher: Mimata

Part: 1c3

Digestion

Researcher: Kishida

Part: psb1c3, idt4

Electrophoresis

Researcher: Kishida, Shibata

Part: psb1c3, idt4

Ethanol Precipitation

Researcher: Kishida, Shibata

Part: psb1c3, idt4

Electrophoresis

Researcher: Kishida, Shibata, Mimata

Part: psb1c3, idt4

Electrophoresis

Researcher: Hirao

Part: idt1, idt2, idt3, idt4, idt5, idt7, idt2 - idt3

Ligation

Researcher: Shibata, Kishida, Mimata

Part: psb1c3 & idt4

PCR (2 STEP)

Researcher: Mimata

Part: idt1, idt2, idt3, idt4, idt5, idt7

Ethanol Precipitation

Researcher: Kishida, Shibata

Part: idt4 on psb1c3

Electrophoresis

Researcher: Kishida

Part: idt4 on psb1c3

Electrophoresis

Researcher: Kishida, Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt7

PEG Precipitation

Researcher: Fujita, Mimata, Shibata, Kono

Part: psb1c3

Electrophoresis

Researcher: Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt7

Dephosphorylation

Researcher: Shibata, Kono, Mimata

Part: psb1c3

Digestion

Researcher: Shibata, Kono, Mimata

Part: idt4

2016/09/06

Ethanol Precipitation

Researcher: Mimata

Part: idt4

Electrophoresis

Researcher: Mimata, Kono

Part: idt4, psb1c3

Electrophoresis

Researcher: Kono

Part: idt1, idt2, idt3, idt4, idt5, idt7

Mini-prep

Researcher: Kono, Shibata

Part: psb1c3

Digestion

Researcher: Mimata, Shibata

Part: psb1c3

PEG Precipitation

Researcher: Kono

Part: psb1c3

Ethanol Precipitation

Researcher: Mimata

Part: psb1c3

Dephosphorylation

Researcher: Mimata

Part: psb1c3

Electrophoresis

Researcher: Mimata, Shibata, Kono

Part: psb1c3

Electrophoresis

Researcher: Mimata, Shibata, Kono

Part: psb1c3

Ligation

Researcher: Shibata, Mimata, Kono

Part: idt4 & psb1c3

Electrophoresis

Researcher: Mimata, Shibata

Part: idt4 on psb1c3

Transformation

Researcher: Mimata, Shibata

Part: idt4 on psb1c3

PCR (2 STEP)

Researcher: Kishida, Hirao

Part: idt6

Electrophoresis

Researcher: Kishida, Hirao

Part: idt6

Electrophoresis

Researcher: Hirao

Part: idt2 - idt3

Gel Extract

Researcher: Kono, Hirao

Part: idt2 - idt3

Electrophoresis

Researcher: Hirao, Mimata

Part: idt2 - idt3

Ethanol Precipitation

Researcher: Kishida, Mimata

Part: idt6

PCR (2 STEP)

Researcher: Hirao

Part: idt2 - idt3

Digestion

Researcher: Mimata, Hirao

Part: idt2 - idt3, pet

Ethanol Precipitation

Researcher: Hirao

Part: idt2 - idt3, pet

Electrophoresis

Researcher: Hirao, Mimata

Part: idt2 - idt3, pet

Electrophoresis

Researcher: Shibata, Mimata, Kono

Part: idt2 - idt3, pet

2016/09/07

Gel Extract

Researcher: Kono, Shibata, Mimata

Part: idt2 - idt3

Electrophoresis

Researcher: Shibata, Mimata, Kono

Part: idt2 - idt3

Colony PCR

Researcher: Shibata, Mimata, Kono

Part: idt4 on 1c3

Digestion

Researcher: Mimata, Kono, Shibata

Part: idt2 - idt3

Electrophoresis

Researcher: Shibata, Mimata, Kono

Part: idt4

Ethanol Precipitation

Researcher: Mimata, Kono

Part: idt2 - idt3

Electrophoresis

Researcher: Mimata, Kono

Part: idt2 - idt3

Dephosphorylation

Researcher: Shibata

Part: pet

Ligation

Researcher: Shibata

Part: pet & idt2 - idt3

Colony PCR

Researcher: Shibata

Part: idt4

Electrophoresis

Researcher: Shibata

Part: idt4

Electrophoresis

Researcher: Shibata

Part: idt2 - idt3 on pet, pet

Digestion

Researcher: Kishida

Part: 1c3, idt1, idt5

Ethanol Precipitation

Researcher: Kishida

Part: 1c3

Ethanol Precipitation

Researcher: Mimata

Part: 1c3, idt1, idt5

Electrophoresis

Researcher: Kishida

Part: 1c3

Digestion

Researcher: Mimata

Part: idt2, idt3, idt6, idt7, 1c3

Colony PCR

Researcher: Mimata

Part: idt4

Digestion

Researcher: Kishida

Part: 1c3, idt1, idt5

Ethanol Precipitation

Researcher: Mimata

Part: 1c3, idt2, idt3, idt6, idt7

Dephosphorylation

Researcher: Mimata

Part: 1c3

Ligation

Researcher: Mimata

Part: 1c3

Electrophoresis

Researcher: Mimata

Part: idt2, idt3, idt6, idt7, 1c3

Ethanol Precipitation

Researcher: Mimata

Part: 1c3, idt1, idt5

Electrophoresis

Researcher: Kishida

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Electrophoresis

Researcher: Kishida

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Electrophoresis

Researcher: Kishida, Mimata

Part: idt4

Ligation

Researcher: Mimata

Part: idt2 & idt3, idt6 & 1c3, idt7 & 1c3

Electrophoresis

Researcher: Mimata

Part: idt1, idt5, 1c3

Gel Extract

Researcher: Kishida, Hirao

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Transformation

Researcher: Mimata

Part: 1c3

Ligation

Researcher: Hirao

Part: idt2 & idt3, idt6 & 1c3, idt7 & 1c3

Electrophoresis

Researcher: Mimata, Hirao

Part: idt2 - idt3, idt6 on 1c3, idt7 on 1c3

2016/09/08

Gel Extract

Researcher: Mimata, Hirao

Part: idt2 - idt3, idt7 on 1c3

Transformation

Researcher: Hirao

Part: idt7 on 1c3

Digestion

Researcher: Mimata, Hirao

Part: idt2 - idt3

Ethanol Precipitation

Researcher: Shibata, Mimata

Part: idt1, idt2, idt3, idt5, idt6, idt7, idt2 - idt3, 1c3

Electrophoresis

Researcher: Shibata, Mimata

Part: idt1, idt2, idt3, idt5, idt6, idt7, idt2 - idt3, 1c3

Digestion

Researcher: Shibata

Part: idt1, idt2, idt3, idt5, idt6, idt7, 1c3

Ligation

Researcher: Hirao, Mimata, Shibata

Part: idt1 & 1c3, idt2 & idt3, idt5 & 1c3, idt6 & 1c3, idt7 & 1c3, idt2 - idt3 & pet

Digestion

Researcher: Kishida

Part: idt1, idt2, idt3, idt5, idt6, idt7, 1c3, pet

Ethanol Precipitation

Researcher: Kishida

Part: idt1, idt2, idt3, idt5, idt6, idt7, 1c3

Electrophoresis

Researcher: Kishida, Mimata

Part: idt1 on 1c3, idt5 on 1c3, idt6 on 1c3, idt7 on 1c3, idt1, idt2, idt3, idt5, idt6, idt7, idt2 - idt3 on pet, 1c3

Colony PCR

Researcher: Hirao

Part: 1c3

Ethanol Precipitation

Researcher: Kishida

Part: pet

Ethanol Precipitation

Researcher: Mimata, Hirao

Part: idt1 on 1c3, idt5 on 1c3, idt6 on 1c3, idt7 on 1c3, idt2 - idt3 on pet, idt2 - idt3

Dephosphorylation

Researcher: Kishida, Hirao

Part: 1c3, pet

Transformation

Researcher: Hirao

Part: idt2 - idt3 on 1c3, idt5 on 1c3

Ligation

Researcher: Hirao

Part: idt6 & 1c3

Electrophoresis

Researcher: Mimata

Part: 1c3

Transformation

Researcher: Mimata

Part: idt2 - idt3 on pet

Digestion

Researcher: Shibata

Part: idt1, idt5, 1c3

Colony PCR

Researcher: Mimata

Part: idt7

2016/09/09

Ethanol Precipitation

Researcher: Shibata

Part: rada, p11

Electrophoresis

Researcher: Mimata

Part: idt6 on 1c3, idt7

Transformation

Researcher: Mimata

Part: idt6 on 1c3

Ethanol Precipitation

Researcher: Shibata

Part: idt1, idt5, 1c3

Digestion

Researcher: Mimata

Part: idt2, idt3, idt6, idt7, 1c3

Electrophoresis

Researcher: Shibata

Part: idt1, idt5, 1c3

Digestion

Researcher: Shibata

Part: rada, p11

Ethanol Precipitation

Researcher: Shibata

Part: rada, p11

Ethanol Precipitation

Researcher: Mimata

Part: idt2, idt3, idt6, idt7, 1c3

Electrophoresis

Researcher: Mimata

Part: pet

Electrophoresis

Researcher: Shibata

Part: rada, p11

Ligation

Researcher: Shibata

Part: idt5, 1c3

Gel Extract

Researcher: Mimata

Part: pet

Electrophoresis

Researcher: Mimata

Part: idt2, idt3, idt6, idt7, 1c3, pet

Dephosphorylation

Researcher: Mimata

Part:1c3, pet

Ligation

Researcher: Shibata

Part: rada & 1c3, p11 & 1c3

Electrophoresis

Researcher: Mimata

Part: 1c3, pet

Ligation

Researcher: Mimata

Part: idt6 & 1c3, idt7 & 1c3

PCR (2 STEP)

Researcher: Kishida

Part: rada, p11

Electrophoresis

Researcher: Kishida

Part: idt5 on 1c3, idt6 on 1c3, idt7 on 1c3, rada on 1c3, p11 on 1c3

Colony PCR

Researcher: Mimata

Part: idt5, idt2 - idt3, idt7

Liquid Culture

Researcher: Kono

Part: 1c3

Colony PCR

Researcher: Kono

Part: idt5

PCR Purification

Researcher: Kishida

Part: rada, p11

Transformation

Researcher: Mimata

Part: idt5 on 1c3, idt6 on 1c3, idt7 on 1c3

Ethanol Precipitation

Researcher: Mimata, Kono

Part: idt1, idt2, idt3, idt4, idt5, idt7

Electrophoresis

Researcher: Mimata

Part: idt5, idt7, idt2 - idt3

Electrophoresis

Researcher: Kono

Part: idt1, idt2, idt3, idt4, idt5, idt7

Electrophoresis

Researcher: Kono

Part: idt5

Electrophoresis

Researcher: Mimata

Part: idt6

Ethanol Precipitation

Researcher: Mimata

Part: idt1, idt2, idt3, pet

2016/09/10

Digestion

Researcher: Kono

Part: idt5, idt7, 1c3

Ethanol Precipitation

Researcher: Kono

Part: idt5, idt7, 1c3

PCR (3 STEP)

Researcher: Shibata

Part: rada, p11

Electrophoresis

Researcher: Shibata

Part: rada, p11

Digestion

Researcher: Shibata

Part: 1c3

Ethanol Precipitation

Researcher: Shibata

Part: 1c3

PCR Purification

Researcher: Shibata

Part: rada, p11

Electrophoresis

Researcher: Shibata

Part: idt1, idt2, idt3, idt5, idt7, 1c3, pet, rada, p11

Mini-prep

Researcher: Shibata

Part: 1c3

PEG Precipitation

Researcher: Shibata

Part: 1c3

PCR (3 STEP)

Researcher: Mimata, Shibata

Part: idt5, idt6, idt7

Ligation

Researcher: Mimata

Part: idt2 & idt3

2016/09/11

Ethanol Precipitation

Researcher: Shibata

Part: rada, p11

Dephosphorylation

Researcher: Mimata

Part: pet

Electrophoresis

Researcher: Mimata

Part: idt5, idt6, idt7

Electrophoresis

Researcher: Shibata

Part: 1c3

Electrophoresis

Researcher: Shibata

Part: rada, p11

Electrophoresis

Researcher: Mimata

Part: idt2 - idt3, pet

Gel Extract

Researcher: Mimata

Part: 1c3

Ligation

Researcher: Mimata

Part: idt1 & 1c3

Gel Extract

Researcher: Mimata

Part: idt2 - idt3

Electrophoresis

Researcher: Mimata

Part: idt2 - idt3, 1c3

PCR (3 STEP)

Researcher: Shibata

Part: rada, p11

Electrophoresis

Researcher: Shibata

Part: rada, p11

PCR Purification

Researcher: Shibata

Part: rada, p11

Ethanol Precipitation

Researcher: Shibata

Part: rada, p11

Electrophoresis

Researcher: Shibata

Part: rada, p11

Ethanol Precipitation

Researcher: Shibata

Part: rada, p11

Electrophoresis

Researcher: Shibata

Part: rada, p11

Transformation

Researcher: Mimata

Part: idt1 on 1c3

Digestion

Researcher: Mimata

Part: idt4, 1c3

Transformation

Researcher: Mimata

Part: pet

Ethanol Precipitation

Researcher: Mimata

Part: idt4, 1c3

Electrophoresis

Researcher: Mimata

Part: idt4, 1c3

Dephosphorylation

Researcher: Mimata

Part: 1c3

2016/09/12

Ligation

Researcher: Mimata

Part: idt4 & 1c3

Electrophoresis

Researcher: Mimata

Part: idt4 on 1c3

Transformation

Researcher: Mimata

Part: idt4 on 1c3

Electrophoresis

Researcher: Nishimura

Part: pet, 1c3

PCR (2 STEP)

Researcher: Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Colony PCR

Researcher: Shibata

Part: idt1

Digestion

Researcher: Nishimura

Part: 1c3

Electrophoresis

Researcher: Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Gel Extract

Researcher: Nishimura, Shibata

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Electrophoresis

Researcher: Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Gel Extract

Researcher: Nishimura, Shibata

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Electrophoresis

Researcher: Nishimura

Part: 1c3, idt1, idt2, idt3, idt4, idt5, idt6, idt7

2016/09/13

Digestion

Researcher: Nishimura, Mimata

Part: 1c3

Liquid Culture

Researcher: Mimata

Part: 1c3

Transformation

Researcher: Mimata

Part: 1c3

Electrophoresis

Researcher: Mimata

Part: 1c3

Electrophoresis

Researcher: Mimata, Nishimura

Part: 1c3

Digestion

Researcher: Nishimura, Mimata

Part: 1c3

Electrophoresis

Researcher: Mimata, Nishimura

Part: 1c3

Digestion

Researcher: Nishimura, Mimata

Part: idt1, idt6, idt7

PCR (2 STEP)

Researcher: Nishimura, Hirao

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Electrophoresis

Researcher: Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Gel Extract

Researcher: Mimata, Hirao, Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Digestion

Researcher: Nishimura

Part: idt2, idt3

Electrophoresis

Researcher: Nishimura

Part: idt1, idt2, idt3, idt4, idt5, idt6, idt7

Ethanol Precipitation

Researcher: Mimata, Nishimura

Part: idt1, idt6, idt7

Electrophoresis

Researcher: Nishimura, Kishimoto

Part: idt1, idt6, idt7

PCR (2 STEP)

Researcher: Nishimura

Part: rada, p11

Electrophoresis

Researcher: Nishimura, Kishimoto

Part: rada, p11

Ligation

Researcher: Nishimura

Part: idt1 & 1c3, idt7 & 1c3, idt6 & idt5 on 1c3

Electrophoresis

Researcher: Kono, Hirao

Part: idt1 on 1c3, idt7 on 1c3

2016/09/14

Transformation

Researcher: Shibata, Kono

Part: idt1 on 1c3, idt7 on 1c3

Digestion

Researcher: Kono, Shibata

Part: 1c3

Electrophoresis

Researcher: Nishimura, Kishimoto, Kishida, Hirao

Part: idt2, idt3, 1c3

Ethanol Precipitation

Researcher: Nishimura, Kishida, Hirao, Kishimoto

Part: idt2, idt3

Digestion

Researcher: Nishimura, Hirao, Kishimoto

Part: pet

Ethanol Precipitation

Researcher: Nishimura, Kishida, Hirao, Kishimoto

Part: idt2, idt3, pet

Transformation

Researcher: Nishimura

Part: idt5 - idt6 on 1c3

Digestion

Researcher: Nishimura

Part: idt3

PCR (2 STEP)

Researcher: Kishida, Nishimura, Kishimoto

Part: idt1, idt2, idt3, idt6, idt7

Ethanol Precipitation

Researcher: Nishimura, Kishida

Part: idt3

Electrophoresis

Researcher: Nishimura

Part: idt1, idt2, idt3, idt6, idt7

Gel Extract

Researcher: Nishimura, Mimata, Kishida

Part: idt1, idt2, idt3, idt6, idt7

Ligation

Researcher: Nishimura

Part: idt2 & idt3

Colony PCR

Researcher: Mimata, Hirao, Shibata

Part: idt7

Electrophoresis

Researcher: Shibata

Part: idt1, idt2, idt3, idt6

Electrophoresis

Researcher: Shibata, Hirao, Noda, Kono

Part: idt7

2016/09/15

Liquid Culture

Researcher: Shibata, Noda, Kono

Part: 1c3

PCR (2 STEP)

Researcher: Kono, Noda

Part: idt7

Electrophoresis

Researcher: Shibata, Noda, Kono

Part: idt1

Electrophoresis

Researcher: Shibata, Kono

Part: idt7

Gel Extract

Researcher: Shibata, Kono, Noda

Part: idt7

Electrophoresis

Researcher: Shibata, Kono, Noda

Part: idt7

Colony PCR

Researcher: Fujita

Part: idt6

Electrophoresis

Researcher: Fujita

Part: idt2 - idt3

Gel Extract

Researcher: Fujita

Part: idt2 - idt3

Electrophoresis

Researcher: Fujita

Part: idt2 - idt3

Electrophoresis

Researcher: Fujita

Part: idt6

Digestion

Researcher: Fujita

Part: idt2, idt3

Colony PCR

Researcher: Fujita

Part: idt5 - idt6

Ethanol Precipitation

Researcher: Nishimura

Part: idt2, idt3

Electrophoresis

Researcher: Kishimoto, Nishimura

Part: idt2, idt3

Colony PCR

Researcher: Nishimura

Part: idt5 - idt6

Colony PCR

Researcher: Fujita

Part: idt5 - idt6

Mini-prep

Researcher: Nishimura, Kishimoto

Part: idt7 on 1c3

Mini-prep

Researcher: Fujita

Part: idt1 on 1c3, 1c3

PEG Precipitation

Researcher: Fujita, Hirao

Part: idt1 on 1c3, idt7 on 1c3, 1c3

Electrophoresis

Researcher: Fujita, Hirao

Part: idt5 - idt6

Electrophoresis

Researcher: Fujita, Hirao

Part: idt7 on 1c3, idt1 on 1c3, 1c3

PCR (3 STEP)

Researcher: Fujita, Hirao

Part: idt1, idt7

Digestion

Researcher: Fujita, Hirao

Part: 1c3, idt4

2016/09/16

Electrophoresis

Researcher: Fujita, Hirao

Part: 1c3, idt4

Ethanol Precipitation

Researcher: Mimata, Fujita, Hirao

Part: idt1, idt7

Sequencing

Researcher: Fujita, Hirao, Mimata

Part: idt1, idt7

Ligation

Researcher: Hirao, Mimata, Fujita

Part: idt4 & 1c3

Electrophoresis

Researcher: Mimata, Hirao, Kono

Part: idt4 on 1c3

Transformation

Researcher: Mimata, Kono

Part: idt4 on 1c3

Colony PCR

Researcher: Nishimura

Part: idt5 - idt6

Ligation

Researcher: Kono, Nishimura

Part: idt2 & idt3

PEG Precipitation

Researcher: Kono

Part: idt1 on 1c3

Electrophoresis

Researcher: Kono, Fujita, Nishimura

Part: idt1 on 1c3

Electrophoresis

Researcher: Kono, Fujita, Nishimura

Part: idt5 - idt6 on 1a3

Sequencing

Researcher: Fujita, Kishida

Part: idt1, idt7

Liquid Culture

Researcher: Nishimura

Part: idt5 on 1a3, idt6 on 1a3

Ligation

Researcher: Nishimura

Part: idt6 & 1c3

Ethanol Precipitation

Researcher: Fujita, Hirao, Shibata

Part: idt1, idt7

Colony PCR

Researcher: Kishida, Fujita, Shibata, Hirao

Part: idt1, idt4, idt7

Transformation

Researcher: Fujita

Part: idt6 on 1c3

2016/09/17

Electrophoresis

Researcher: Fujita, Hirao, Mimata

Part: idt1, idt4, idt7

Liquid Culture

Researcher: Mimata

Part: idt1 on 1c3, idt7 on 1c3

Digestion

Researcher: Nishimura

Part: 1c3

Mini-prep

Researcher: Nishimura

Part: idt1 on 1c3, idt5 - idt6 on 1a3

PEG Precipitation

Researcher: Nishimura

Part: idt1 on 1c3, idt5 - idt6 on 1a3

Electrophoresis

Researcher: Nishimura, Kishida

Part: idt1 on 1c3, idt5 - idt6 on 1a3, idt4

Ligation

Researcher: Nishimura

Part: idt4 & 1c3

Electrophoresis

Researcher: Kishida

Part: idt1 on 1c3, idt5 - idt6 on 1a3

Transformation

Researcher: Kishida

Part: idt4 on 1c3

Sequencing

Researcher: Shidata, Nishimura

Part: idt1, idt7

Colony PCR

Researcher: Nishimura

Part: idt4

Transformation

Researcher: Nishimura

Part: idt4 on 1c3

Colony PCR

Researcher: Nishimura, Shibata

Part: idt6

Digestion

Researcher: Shibata

Part: idt4, 1c3

Ethanol Precipitation

Researcher: Shibata, Nishimura

Part: idt4, 1c3

Electrophoresis

Researcher: Kono, Nishimura, Shibata

Part: idt4, 1c3

Ethanol Precipitation

Researcher: Mimata, Kono

Part: idt1, idt5 - idt6, idt7

Digestion

Researcher: Mimata, Kono

Part: idt4

2016/09/18

Ethanol Precipitation

Researcher: Shibata, Mimata, Kono

Part: idt4

Mini-prep

Researcher: Mimata, Shibata, Kono

Part: idt1 on 1c3, idt7 on 1c3

Electrophoresis

Researcher: Kono, Mimata, Shibata

Part: idt1 on 1c3, idt7 on 1c3, idt4

PEG Precipitation

Researcher: Mimata, Shibata, Kono

Part: idt1 on 1c3, idt7 on 1c3

Electrophoresis

Researcher: Kono, Mimata, Fujita

Part: idt6

Ligation

Researcher: Fujita, Kono, Mimata

Part: idt4 & 1c3

Liquid Culture

Researcher: Mimata, Kono

Part: idt6 on 1c3

Electrophoresis

Researcher: Kono, Mimata, Fujita

Part: idt1 on 1c3, idt7 on 1c3

Sequencing

Researcher: Mimata, Fujita, Kono

Part: idt1, idt7

Electrophoresis

Researcher: Mimata, Fujita

Part: idt4 on 1c3

Liquid Culture

Researcher: Kono

Part: idt1 on 1c3

Transformation

Researcher: Mimata

Part: idt4 on 1c3

Ethanol Precipitation

Researcher: Fujita

Part: idt1, idt7

Colony PCR

Researcher: Nishimura, Hirao, Kishimoto

Part: idt4

Digestion

Researcher: Nishimura, Hirao, Kishimoto

Part: idt7

Colony PCR

Researcher: Nishimura, Kishimoto

Part: idt7

Ethanol Precipitation

Researcher: Nishimura, Kishimoto

Part: idt7

Electrophoresis

Researcher: Mimata

Part: idt7

Electrophoresis

Researcher: Esashi

Part: idt4

Mini-prep

Researcher: Mimata, Hirao

Part: idt6

Electrophoresis

Researcher: Noda

Part: idt6

Ligation

Researcher: Mimata, Hirao

Part: idt7 & 1c3

Mini-prep

Researcher: Mimata, Hirao

Part: idt1 on 1c3

Electrophoresis

Researcher: Noda

Part: idt1 on 1c3

PEG Precipitation

Researcher: Mimata, Hirao

Part: idt1 on 1c3

Electrophoresis

Researcher: Esashi

Part: idt7 on 1c3

Sequencing

Researcher: Mimata, Hirao

Part: idt7

Electrophoresis

Researcher: Hirao, Mimata

Part: idt1 on 1c3, idt7 on 1c3

Transformation

Researcher: Mimata, Hirao

Part: idt7 on 1c3

2016/09/19

Sequencing

Researcher: Mimata, Hirao

Part: idt1

Colony PCR

Researcher: Mimata, Shibata, Hirao

Part: idt4

Electrophoresis

Researcher: Shibata

Part: idt4

Liquid Culture

Researcher: Mimata

Part: idt7 on 1c3

Ethanol Precipitation

Researcher: Mimata, Shibata, Hirao

Part: idt1, idt7

Liquid Culture

Researcher: Nishimura

Part: idt4 on 1c3

PEG Precipitation

Researcher: Shibata

Part: idt6 on 1c3

Electrophoresis

Researcher: Shibata

Part: idt6 on 1c3

Sequencing

Researcher: Shibata

Part: idt6

Ethanol Precipitation

Researcher: Nishimura

Part: idt6

Sequencing

Researcher: Nishimura

Part: idt1, idt7

PEG Precipitation

Researcher: Nishimura, Kishida

Part: idt1 on 1c3

Mini-prep

Researcher: Nishimura, Kishida

Part: idt4 on 1c3

Liquid Culture

Researcher: Nishimura

Part: idt1 on 1c3

PEG Precipitation

Researcher: Nishimura

Part: idt1 on 1c3

Electrophoresis

Researcher: Nishimura

Part: idt4 on 1c3

Electrophoresis

Researcher: Nishimura

Part: idt1 on 1c3

Ethanol Precipitation

Researcher: Nishimura

Part: idt1, idt7

Electrophoresis

Researcher: Nishimura

Part: idt1 on 1c3, idt4 on 1c3

Mini-prep

Researcher: Nishimura, Mimata

Part: idt7 on 1c3

Electrophoresis

Researcher: Nishimura, Mimata

Part: idt7 on 1c3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: idt7 on 1c3

Electrophoresis

Researcher: Mimata

Part: idt7 on 1c3

Colony PCR

Researcher: Mimata, Fujita

Part: idt6, idt7

Sequencing

Researcher: Mimata, Fujita

Part: idt1, idt4, idt7

2016/09/20

Electrophoresis

Researcher: Mimata, Fujita

Part: idt6, idt7

Ethanol Precipitation

Researcher: Mimata, Fujita

Part: idt1, idt4, idt7

Mini-prep

Researcher: Mimata, Fujita

Part: idt4 on 1c3

Colony PCR

Researcher: Mimata, Fujita

Part: idt6, idt7

Mini-prep

Researcher: Mimata, Fujita

Part: idt1 on 1c3

Electrophoresis

Researcher: Mimata, Fujita

Part: idt1 on 1c3

Electrophoresis

Researcher: Nishimura

Part: idt5, idt6

Digestion

Researcher: Nishimura

Part: idt5

Ethanol Precipitation

Researcher: Nishimura

Part: idt5

Colony PCR

Researcher: Nishimura

Part: idt6, idt7

Electrophoresis

Researcher: Nishimura

Part: idt5

Ligation

Researcher: Nishimura

Part: idt5 & 1c3

Electrophoresis

Researcher: Nishimura

Part: idt6, idt7

Transformation

Researcher: Nishimura

Part: idt5 on 1c3

PEG Precipitation

Researcher: Nishimura

Part: idt1 on 1c3, idt4 on 1c3, idt5 on 1a3

Electrophoresis

Researcher: Nishimura

Part: idt4 on 1c3

Sequencing

Researcher: Fujita, Kono

Part: idt1, idt4, idt7, idt5 - idt6

2016/09/21

PEG Precipitation

Researcher: Fujita, Kono

Part: idt1 on 1c3, idt5 on 1a3, idt5 - idt6 on 1a3

Colony PCR

Researcher: Fujita, Kono

Part: idt6

Electrophoresis

Researcher: Fujita, Kono, Hirao

Part: idt1 on 1c3, idt5 on 1a3, idt5 - idt6 on 1a3

Ethanol Precipitation

Researcher: Shibata, Mimata, Hirao

Part: idt1, idt4, idt7, idt5 - idt6

Sequencing

Researcher: Mimata, Shibata, Hirao

Part: idt1, idt4, idt5 - idt6

Electrophoresis

Researcher: Hirao

Part: idt6

Ethanol Precipitation

Researcher: Shibata, Mimata, Hirao

Part: idt1, idt4, idt5 - idt6

Liquid Culture

Researcher: Nishimura

Part: idt6 on 1c3

Colony PCR

Researcher: Nishimura, Kono

Part: idt7

PEG Precipitation

Researcher: Nishimura

Part: idt1

Electrophoresis

Researcher: Kono

Part: idt7

PEG Precipitation

Researcher: Mimata, Kono

Part: idt7

Electrophoresis

Researcher: Mimata, Kono

Part: idt1, idt7

Sequencing

Researcher: Mimata, Kono, Esashi

Part: idt1, idt4, idt5 - idt6, idt7

2016/09/22

Ethanol Precipitation

Researcher: Mimata, Shibata, Noda

Part: idt1, idt4, idt5 - idt6, idt7

Digestion

Researcher: Mimata

Part: idt5

Digestion

Researcher: Mimata

Part: BBa_I13401

Ethanol Precipitation

Researcher: Mimata, Shibata

Part: BBa_I13401, idt5 on 1a3

Electrophoresis

Researcher: Noda

Part: BBa_I13401, idt5 on 1a3

Mini-prep

Researcher: Nishimura

Part: idt6 on 1c3

Electrophoresis

Researcher: Nishimura

Part: idt6 on 1a3

Transformation

Researcher: Nishimura

Part: BBa_I13401 on 1c3

PEG Precipitation

Researcher: Nishimura

Part: idt6 on 1c3

Sequencing

Researcher: Nishimura

Part: idt1, idt4, idt5 - idt6, idt6, idt7

Electrophoresis

Researcher: Hirao

Part: idt6 on 1a3

Colony PCR

Researcher: Nishimura

Part: idt7

Ethanol Precipitation

Researcher: Nishimura

Part: idt1, idt4, idt5 - idt6, idt6, idt7

Electrophoresis

Researcher: Hirao, Nishimura

Part: idt7 on 1c3

Liquid Culture

Researcher: Nishimura, Hirao

Part: idt1 on 1c3

PEG Precipitation

Researcher: Nishimura

Part: idt6 on 1c3

2016/09/23

Colony PCR

Researcher: Fujita, Shibata

Part: idt6

Digestion

Researcher: Nishimura, Shibata

Part: idt4, 1c3

Electrophoresis

Researcher: Nishimura

Part: idt6 on 1c3

Electrophoresis

Researcher: Shibata

Part: idt4, 1c3

Electrophoresis

Researcher: Fujita

Part: idt6

Ethanol Precipitation

Researcher: Shibata

Part: idt4

Transformation

Researcher: Nishimura

Part: BBa_B0015 on 1c3

Ligation

Researcher: Shibata, Fujita

Part: idt4 & 1c3

Transformation

Researcher: Shibata

Part: idt4 on 1c3

Mini-prep

Researcher: Nishimura

Part: idt5 on 1a3

Liquid Culture

Researcher: Nishimura

Part: BBa_I13401 on 1a2

PEG Precipitation

Researcher: Nishimura

Part: idt5 on 1a3

Colony PCR

Researcher: Nishimura

Part: idt4

Electrophoresis

Researcher: Nishimura

Part: idt5 on 1a3

Mini-prep

Researcher: Nishimura, Kishimoto

Part: idt1 on 1c3

PEG Precipitation

Researcher: Nishimura, Kishida, Kishimoto

Part: idt1 on 1c3

Transformation

Researcher: Nishimura, Kishimoto

Part: BBa_B0015 on 1c3

Electrophoresis

Researcher: Nishimura

Part: idt1 on 1c3

Electrophoresis

Researcher: Hirao

Part: idt4

Liquid Culture

Researcher: Nishimura

Part: idt5 - idt6 on 1a3, idt7 on 1c3

Digestion

Researcher: Nishimura

Part: idt5, 1c3

2016/09/24

Colony PCR

Researcher: Mimata

Part: idt4

Sequencing

Researcher: Nishimura

Part: idt1, idt4, idt6

Ethanol Precipitation

Researcher: Fujita

Part: idt5, 1c3

Electrophoresis

Researcher: Fujita

Part: idt5, 1c3

Mini-prep

Researcher: Mimata

Part: idt5 - idt6 on 1a3, idt7 on 1c3

PEG Precipitation

Researcher: Mimata

Part: idt5 - idt6 on 1a3, idt7 on 1c3

Electrophoresis

Researcher: Fujita

Part: idt5 - idt6 on 1a3, idt7 on 1c3, 1c3

Digestion

Researcher: Nishimura

Part: 1c3

Digestion

Researcher: Mimata, Fujita

Part: idt5 on 1a3, idt7 on 1c3

Electrophoresis

Researcher: Fujita, Mimata

Part: idt4

Liquid Culture

Researcher: Fujita

Part: idt1 on 1c3, BBa_I13401 on 1a2

Electrophoresis

Researcher: Fujita

Part: idt5 - idt6 on 1a3, idt7 on 1c3

Electrophoresis

Researcher: Hirao, Shibata

Part: idt5 on 1a3, idt7 on 1c3

Ligation

Researcher: Hirao, Shibata

Part: idt5 & idt7 on 1c3, idt5 & 1c3

Electrophoresis

Researcher: Hirao, Shibata

Part: idt5 - idt7 on 1c3, idt5 on 1c3

Transformation

Researcher: Kishida

Part: idt5 on 1c3, BBa_B0015 on 1c3

Sequencing

Researcher: Nishimura

Part: idt1, idt4, idt6

Digestion

Researcher: Shibata, Nishimura

Part: idt7 on 1c3

Electrophoresis

Researcher: Shibata

Part: idt7 on 1c3

Liquid Culture

Researcher: Shibata

Part: idt4 on 1c3

2016/09/25

Ligation

Researcher: Shibata

Part: idt5 & idt7 on 1c3

Transformation

Researcher: Mimata

Part: idt5 - idt7 on 1c3

Colony PCR

Researcher: Mimata

Part: idt4

Electrophoresis

Researcher: Mimata

Part: idt4

Mini-prep

Researcher: Mimata, Fujita

Part: idt1 on 1c3, BBa_I13401 on 1a2

Electrophoresis

Researcher: Mimata

Part: idt1 on 1c3, BBa_I13401 on 1a2

PEG Precipitation

Researcher: Nishimura, Fujita

Part: idt1 on 1c3, BBa_I13401 on 1a2

PEG Precipitation

Researcher: Mimata, Fujita

Part: idt4 on 1c3

Transformation

Researcher: Mimata, Hirao

Part: BBa_B0015 on 1c3, BBa_I13401 on 1c3

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_I13401, idt5 - idt6

Electrophoresis

Researcher: Hirao

Part: idt4

Colony PCR

Researcher: Mimata

Part: idt5

Electrophoresis

Researcher: Hirao

Part: BBa_I13401, idt5 - idt6

Liquid Culture

Researcher: Nishimura

Part: idt6 on 1c3, idt7 on 1c3

Electrophoresis

Researcher: Nishimura

Part: idt5

PCR (3 STEP)

Researcher: Nishimura

Part: idt7, idt5 - idt6

Liquid Culture

Researcher: Hirao

Part: idt5 on 1c3, idt5 - idt6 on 1c3

2016/09/26

Ligation

Researcher: Nishimura, Hirao

Part: idt5 & idt7 on 1c3

Electrophoresis

Researcher: Hirao

Part: idt5 - idt6, idt7

Transformation

Researcher: Mimata

Part: idt5 - idt7 on 1c3

Electrophoresis

Researcher: Mimata

Part: idt5 - idt6, idt7

Gel Extract

Researcher: Mimata

Part: idt5 - idt6, idt7

Electrophoresis

Researcher: Mimata

Part: idt5 - idt6, idt7

Transformation

Researcher: Mimata

Part: BBa_B0015 on 1c3, BBa_I13401 on 1c3

Mini-prep

Researcher: Mimata

Part: idt6 on 1c3, idt7 on 1c3

Electrophoresis

Researcher: Mimata

Part: idt6 on 1c3, idt7 on 1c3

PEG Precipitation

Researcher: Mimata

Part: idt6 on 1c3, idt7 on 1c3

Electrophoresis

Researcher: Mimata

Part: idt6 on 1c3, idt7 on 1c3

Colony PCR

Researcher: Mimata, Shibata

Part: idt4

Digestion

Researcher: Nishimura

Part: idt5 on 1c3, BBa_I13401

PCR (3 STEP)

Researcher: Nishimura

Part: BBa_I13401

Electrophoresis

Researcher: Nishimura

Part: idt5 on 1a3, BBa_I13401

Mini-prep

Researcher: Hirao, Shibata, Kishida

Part: idt5 on 1c3, idt5 - idt6 on 1a3

Electrophoresis

Researcher: Noda

Part: idt4 on 1c3, idt5 on 1c3, idt5 - idt6 on 1a3, BBa_I13401

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_I13401

PEG Precipitation

Researcher: Nishimura

Part: idt5 on 1c3, idt5 - idt6 on 1a3

PEG Precipitation

Researcher: Nishimura

Part: idt6 on 1c3, idt7 on 1c3, idt5 - idt6 on 1a3

Digestion

Researcher: Nishimura

Part: 1c3

Liquid Culture

Researcher: Kishimoto

Part: idt4 on 1c3

Electrophoresis

Researcher: Nishimura

Part: idt5 on 1c3, idt5 - idt6 on 1a3, 1c3

Electrophoresis

Researcher: Nishimura, Kishimoto, Kono

Part: idt1 on 1c3, idt5 on 1c3, idt6 on 1c3, idt7 on 1c3, idt5 - idt6 on 1a3

Liquid Culture

Researcher: Nishimura, Kishimoto, Hirao

Part: idt5 on 1c3, idt5 - idt6 on 1a3

2016/09/27

Digestion

Researcher: Mimata

Part: 1c3

Electrophoresis

Researcher: Mimata

Part: 1c3

Colony PCR

Researcher: Mimata

Part: idt5, idt7

Digestion

Researcher: Mimata

Part: 1c3

Transformation

Researcher: Nishimura, Mimata

Part: BBa_B0015

Electrophoresis

Researcher: Mimata

Part: 1c3

Liquid Culture

Researcher: Nishimura

Part: idt5 - idt6 on 1a3

Electrophoresis

Researcher: Mimata

Part: idt5, idt7

Digestion

Researcher: Mimata

Part: BBa_I13401, idt5 on 1a3

Mini-prep

Researcher: Mimata

Part: idt4 on 1c3

Electrophoresis

Researcher: Mimata

Part: idt4 on 1c3, BBa_I13401, idt5 on 1a3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: idt4 on 1c3

Liquid Culture

Researcher: Mimata

Part: idt5 - idt7 on 1c3

Mini-prep

Researcher: Mimata

Part: idt5 - idt6 on 1a3, idt5 on 1c3

Electrophoresis

Researcher: Mimata

Part: idt5 - idt6 on 1a3, idt5 on 1c3, idt4 on 1c3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: idt5 - idt6 on 1a3, idt5 on 1c3

Gel Extract

Researcher: Nishimura

Part: BBa_I13401

Electrophoresis

Researcher: Nishimura

Part: idt5 - idt6 on 1a3, idt5 on 1c3, BBa_I13401

Sequencing

Researcher: Nishimura

Part: idt4, idt5

Ligation

Researcher: Shibata

Part: idt5 on 1a3 & BBa_I13401

PEG Precipitation

Researcher: Fujita, Hirao, Kishimoto

Part: idt5 on 1c3, idt5 - idt6 on 1a3, idt4 on 1c3

Transformation

Researcher: Kishimoto, Hirao

Part: idt5 - BBa_I13401 on 1a3

Liquid Culture

Researcher: Kishimoto, Hirao

Part: idt5 on 1c3, idt4 on 1c3

Electrophoresis

Researcher: Kishimoto, Hirao

Part: idt5 - idt6 on 1a3, idt5 on 1c3, idt4 on 1c3

2016/09/28

Mini-prep

Researcher: Mimata

Part: BBa_B0015 on 1c3, idt5 - idt7 on 1c3

PEG Precipitation

Researcher: Mimata, Nishimura

Part: idt5 - -idt7 on 1c3

Ethanol Precipitation

Researcher: Nishimura

Part: idt4, idt5

Liquid Culture

Researcher: Nishimura, Kishimoto, Kono

Part: idt5 - idt7 on 1c3

Electrophoresis

Researcher: Nishimura, Kishimoto, Kono

Part: idt5 - idt7 on 1c3

Streaking (Single Colony Isolation)

Researcher: Mimata

Part: idt5 - BBa_I13401 on 1a3

Digestion

Researcher: Mimata, Fujita

Part: idt5 on 1a3, idt7

Mini-prep

Researcher: Kishida

Part: idt5 on 1c3, idt4 on 1c3

Electrophoresis

Researcher: Kishida

Part: idt5 on 1c3, idt4 on 1c3

Sequencing

Researcher: Nishimura

Part: idt4, idt5

Electrophoresis

Researcher: Mimata

Part: idt5 on 1a3, idt7

Electrophoresis

Researcher: Kishida

Part: idt5 on 1c3, idt4 on 1c3

Ligation

Researcher: Fujita

Part: idt5 on 1a3 & idt7

Transformation

Researcher: Fujita

Part: idt5 - idt7 on 1a3

2016/09/29

Ethanol Precipitation

Researcher: Nishimura

Part: idt4, idt5

Mini-prep

Researcher: Kono

Part: BBa_B0015 on 1c3, idt5 - idt6 on 1a3

Electrophoresis

Researcher: Mimata, Kono

Part: BBa_B0015 on 1c3, idt5 - idt6 on 1a3

PEG Precipitation

Researcher: Mimata, Kono

Part: BBa_B0015 on 1c3, idt5 - idt6 on 1a3

Streaking (Single Colony Isolation)

Researcher: Kono, Mimata

Part: idt5 - idt7 on 1a3

Electrophoresis

Researcher: Shibata

Part: BBa_B0015 on 1c3, idt5 - idt6 on 1a3

PCR (2 STEP)

Researcher: Nishimura

Part: idt8

Streaking (Single Colony Isolation)

Researcher: Hirao

Part: idt5 - BBa_I13401 on 1a3

Electrophoresis

Researcher: Hirao, Kishida

Part: idt8

PCR (3 STEP)

Researcher: Nishimura, Hirao

Part: idt8

Electrophoresis

Researcher: Nishimura

Part: idt8

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_B0015

Electrophoresis

Researcher: Nishimura

Part: BBa_B0015

Mini-prep

Researcher: Shibata

Part: idt5 - BBa_I13401 on 1a3

PEG Precipitation

Researcher: Shibata

Part: idt5 - BBa_I13401 on 1a3

2016/09/30

Electrophoresis

Researcher: Shibata

Part: idt5 - BBa_I13401 on 1a3

Digestion

Researcher: Nishimura

Part: idt5 - idt6 on 1a3, BBa_B0015 on 1c3

Electrophoresis

Researcher: Mimata

Part: idt5 - idt6 on 1a3, BBa_B0015 on 1c3

Gel Extract

Researcher: Mimata

Part: idt5 - idt6 on 1a3

PCR (STEP DOWN)

Researcher: Mimata

Part: idt8

Electrophoresis

Researcher: Mimata, Shibata

Part: idt5 - idt6 on 1a3

Electrophoresis

Researcher: Mimata, Shibata

Part: idt8

Liquid Culture

Researcher: Mimata

Part: idt5 - BBa_I13401 on 1a3

PEG Precipitation

Researcher: Nishimura

Part: idt5 - idt7 on 1c3, BBa_B0015 on 1c3

Gel Extract

Researcher: Shibata

Part: idt5 - idt6

Electrophoresis

Researcher: Mimata, Shibata

Part: idt5 - idt6 on 1a3, idt5 - idt7 on 1c3, BBa_B0015 on 1c3

Electrophoresis

Researcher: Mimata

Part: idt5 - idt7 on 1c3, BBa_B0015 on 1c3

2016/10/01

PCR (2 STEP)

Researcher: Mimata

Part: idt4

Digestion

Researcher: Mimata

Part: idt6 on 1c3, idt7 on 1c3, 1c3

Gel Free System

Researcher: Mimata, Kishimoto

Part: BBa_J04500

PEG Precipitation

Researcher: Mimata, Kishimoto, Hirao

Part: BBa_J04500 on 1c3

Electrophoresis

Researcher: Mimata, Kishimoto, Hirao

Part: idt4

Electrophoresis

Researcher: Mimata, Hirao

Part: idt6 on 1c3, idt7 on 1c3, 1c3

Electrophoresis

Researcher: Mimata, Hirao

Part: BBa_J04500 on 1c3, BBa_J04500, idt4

Digestion

Researcher: Hirao, Mimata

Part: idt4

Gel Free System

Researcher: Hirao, Mimata

Part: BBa_J04500, BBa_I13401

Transformation

Researcher: Kishida

Part: BBa_R0010 on 1c3, BBa_J04500 on 1c3

Liquid Culture

Researcher: Mimata, Shibata

Part: idt6 on 1c3, idt7 on 1c3

Digestion

Researcher: Hirao, Mimata

Part: idt6 on 1c3, idt7 on 1c3

Electrophoresis

Researcher: Shibata, Hirao, Kishida

Part: 1c3, idt4, idt6 on 1c3, idt7 on 1c3

Ethanol Precipitation

Researcher: Shibata

Part: BBa_J04500, BBa_I13401

Gel Extract

Researcher: Kishida

Part: 1c3

Electrophoresis

Researcher: Shibata

Part: 1c3, BBa_J04500, BBa_I13401

Digestion

Researcher: Shibata

Part: BBa_J04500 on 1c3

Ligation

Researcher: Shibata

Part: 1c3 & idt4

Gel Free System

Researcher: Mimata

Part: BBa_J04500, BBa_I13401

Ethanol Precipitation

Researcher: Shibata

Part: BBa_J04500, BBa_I13401

Transformation

Researcher: Shibata

Part: idt4 on 1c3

2016/10/02

Digestion

Researcher: Shibata

Part: BBa_J04500, BBa_I13401

Electrophoresis

Researcher: Shibata

Part: BBa_J04500 on 1c3, BBa_J04500, BBa_I13401

PEG Precipitation

Researcher: Shibata

Part: BBa_J04500 on 1c3

Electrophoresis

Researcher: Shibata

Part: BBa_J04500 on 1c3, BBa_J04500, BBa_I13401

Digestion

Researcher: Shibata

Part: BBa_J04500 on 1c3

Digestion

Researcher: Shibata

Part: idt7 on 1c3, BBa_J04500, BBa_I13401

Mini-prep

Researcher: Shibata

Part: idt6 on 1c3, idt7 on 1c3

PEG Precipitation

Researcher: Mimata

Part: idt6 on 1c3, idt7 on 1c3

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_J04500 on 1c3, idt7 on 1c3, BBa_J04500, BBa_I13401, idt6 on 1c3

Digestion

Researcher: Mimata

Part: BBa_J04500, BBa_I13401, BBa_I13401 on 1a2

Electrophoresis

Researcher: Mimata

Part: idt6 on 1c3, idt7 on 1c3

Digestion

Researcher: Mimata

Part: idt6 on 1c3

Transformation

Researcher: Mimata, Hirao

Part: BBa_R0010 on 1c3, BBa_J04500 on 1c3

Electrophoresis

Researcher: Mimata, Hirao

Part: idt6 on 1c3, idt7 on 1c3, BBa_J04500, BBa_I13401, BBa_I13401 on 1a2

Electrophoresis

Researcher: Hirao, Fujita

Part: BBa_J04500, BBa_I13401

Gel Extract

Researcher: Hirao, Fujita

Part: BBa_J04500, BBa_I13401

Electrophoresis

Researcher: Hirao, Fujita

Part: BBa_J04500, BBa_I13401

Colony PCR

Researcher: Fujita, Hirao

Part: idt4

Ligation

Researcher: Fujita, Hirao

Part: BBa_J04500 & idt6 on 1c3, BBa_J04500 & idt7 on 1c3, BBa_J04500 & BBa_I13401 on 1a2,

Digestion

Researcher: Fujita, Kishimoto

Part: BBa_I13401, BBa_J04500

Transformation

Researcher: Mimata, Hirao, Fujita, Kishimoto

Part: BBa_J04500 - BBa_I13401 on 1a2, BBa_J04500 - idt6 on 1c3, BBa_J04500 - idt7 on 1c3

Electrophoresis

Researcher: Kishimoto, Mimata, Fujita

Part: BBa_J04500, BBa_I13401

Electrophoresis

Researcher: Kishimoto, Fujita

Part: idt4

Electrophoresis

Researcher: Kono

Part: BBa_J04500, BBa_I13401

Gel Extract

Researcher: Kono

Part: BBa_J04500, BBa_I13401

2016/10/03

Electrophoresis

Researcher: Kono

Part: BBa_J04500, BBa_I13401

Streaking (Single Colony Isolation)

Researcher: Mimata

Part: BBa_J04500 - BBa_I13401 on 1a2

Colony PCR

Researcher: Mimata, Fujita

Part: idt4

Transformation

Researcher: Mimata, Fujita

Part: BBa_J04500 on 1c3

Digestion

Researcher: Mimata, Fujita

Part: BBa_I13401 on 1a2

Electrophoresis

Researcher: Fujita

Part: BBa_I13401 on 1a2, idt4

Colony PCR

Researcher: Mimata, Fujita, Shibata

Part: BBa_J04500 - idt7

Digestion

Researcher: Mimata, Fujita, Shibata

Part: idt4

Electrophoresis

Researcher: Fujita, Kono

Part: idt4

Liquid Culture

Researcher: Shibata

Part: BBa_R0010 on 1c3

Ligation

Researcher: Fujita

Part: idt4 & 1c3, BBa_J04500 & idt6 on 1c3

Electrophoresis

Researcher: Fujita, Kono

Part: BBa_J04500 - idt7

Transformation

Researcher: Fujita

Part: idt4 on 1c3, BBa_J04500 - idt6 on 1c3

Ligation

Researcher: Fujita

Part: BBa_J04500 & idt6 on 1c3, BBa_J04500 & idt7 on 1c3

2016/10/04

Streaking (Single Colony Isolation)

Researcher: Fujita

Part: BBa_J04500 - BBa_I13401 on 1a2

Mini-prep

Researcher: Fujita

Part: BBa_R0010 on 1c3

Electrophoresis

Researcher: Fujita

Part: BBa_R0010 on 1c3

PEG Precipitation

Researcher: Fujita, Mimata

Part: BBa_R0010 on 1c3

Transformation

Researcher: Fujita, Mimata, Shibata

Part: BBa_J04500 - idt6 on 1c3, BBa_J04500 - idt7 on 1c3

Colony PCR

Researcher: Mimata, Shibata

Part: idt4, BBa_J04500 - idt6

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_R0010 on 1c3

PCR (2 STEP)

Researcher: Mimata, Shibata

Part: BBa_J04500

PEG Precipitation

Researcher: Mimata, Shibata

Part: BBa_I13401 on 1a2

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_R0010 on 1c3, BBa_I13401 on 1a2

Electrophoresis

Researcher: Mimata, Shibata

Part: BBa_J04500

Electrophoresis

Researcher: Mimata

Part: idt4

Electrophoresis

Researcher: Shibata

Part: BBa_J04500 - idt6

Colony PCR

Researcher: Fujita, Kono, Kishida

Part: idt4

Gel Extract

Researcher: Shibata

Part: BBa_J04500

Electrophoresis

Researcher: Shibata

Part: BBa_J04500

Digestion

Researcher: Fujita, Kishida

Part: BBa_I13401 on 1a2, BBa_J04500

Electrophoresis

Researcher: Shibata

Part: BBa_I13401 on 1a2, BBa_J04500

Electrophoresis

Researcher: Mimata, Fujita

Part: idt4

Ligation

Researcher: Mimata, Fujita, Kono

Part: BBa_I13401 on 1a2 & BBa_J04500

Streaking (Single Colony Isolation)

Researcher: Kishida, Mimata

Part: BBa_J04500 - BBa_I13401 on 1a2

2016/10/05

Colony PCR

Researcher: Mimata

Part: idt4

Transformation

Researcher: Mimata, Shibata

Part: BBa_J04500 - BBa_I13401 on 1a2, BBa_J04500 on 1c3

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa_J04500 on 1c3

Electrophoresis

Researcher: Mimata

Part: idt4

Colony PCR

Researcher: Mimata, Shibata

Part: idt4

PCR (2 STEP)

Researcher: Mimata, Shibata

Part: gfp

Transformation

Researcher: Mimata, Shibata

Part: BBa_B0034 on 1a2

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa_B0034 on 1a2

Electrophoresis

Researcher: Shibata

Part: idt4, gfp

2016/10/06

Mini-prep

Researcher: Mimata, Shibata

Part: BBa_J04500 on 1c3, BBa_I13401 on 1a2

Electrophoresis

Researcher: Mimata

Part: BBa_J04500 on 1c3, BBa_I13401 on 1a2

PEG Precipitation

Researcher: Mimata

Part: BBa_J04500 on 1c3, BBa_I13401 on 1a2

Electrophoresis

Researcher: Mimata

Part: BBa_J04500 on 1c3, BBa_I13401 on 1a2

PCR (2 STEP)

Researcher: Nishimura, Mimata

Part: idt4, BBa_J04500, BBa_I13401

Electrophoresis

Researcher: Mimata, Fujita

Part: idt4

PCR (2 STEP)

Researcher: Fujita, Mimata, Hirao

Part: idt4, BBa_J04500

Gel Extract

Researcher: Hirao

Part: BBa_I13401

Electrophoresis

Researcher: Hirao, Fujita

Part: idt4 on 1c3, BBa_I13401

Liquid Culture

Researcher: Mimata, Fujita, Hirao

Part: idt1 on 1c3, idt6 on 1c3, idt7 on 1c3

Gel Extract

Researcher: Mimata

Part: idt4 on 1c3

Electrophoresis

Researcher: Fujita

Part: BBa_J04500

2016/10/07

Gel Extract

Researcher: Nishimura

Part: BBa_J04500

Electrophoresis

Researcher: Nishimura

Part: BBa_J04500, idt4 on 1c3

Liquid Culture

Researcher: Nishimura

Part: BBa_J04500 - BBa_I13401 on 1a2

Digestion

Researcher: Nishimura

Part: idt4

Ligation

Researcher: Kishida, Fujita

Part: idt4

Mini-prep

Researcher: Mimata, Shibata

Part: idt1 on 1c3, idt6 on 1c3, idt7 on 1c3

Electrophoresis

Researcher: Mimata, Shibata

Part: idt1 on 1c3, idt6 on 1c3, idt7 on 1c3

PEG Precipitation

Researcher: Mimata, Shibata

Part: idt1 on 1c3, idt6 on 1c3, idt7 on 1c3

Electrophoresis

Researcher: Shibata

Part: idt4

Digestion

Researcher: Fujita, Kishida, Shibata, Mimata

Part: BBa_J04500 on 1c3, idt6, idt7, BBa_I13401

Liquid Culture

Researcher: Nishimura

Part: BBa_B0034 on 1a2, BBa_J04500 - BBa_I13401 on 1a2

Streaking (Single Colony Isolation)

Researcher: Nishimura

Part: BBa_B0034 on 1a2, BBa_J04500 - BBa_I13401 on 1a2

Electrophoresis

Researcher: Fujita

Part: idt1 on 1c3, idt6 on 1c3, idt7 on 1c3, BBa_J04500 on 1c3, idt6, idt7, BBa_I13401

Transformation

Researcher: Fujita, Kishida, Nishimura

Part: idt4

Ethanol Precipitation

Researcher: Fujita

Part: idt1 on 1c3, idt6 on 1c3, idt7 on 1c3

Ligation

Researcher: Shibata, Fujita, Kishida

Part: BBa_J04500 on 1c3 & idt6, BBa_J04500 on 1c3 & idt7, BBa_J04500 on 1c3 & BBa_I13401

Transformation

Researcher: Fujita

Part: BBa_J04500 - idt6 on 1c3, BBa_J04500 - idt7 on 1c3, BBa_J04500 - BBa_I13401on 1c3

2016/10/08

Liquid Culture

Researcher: Nishimura

Part: BBa_J04500 - BBa_I13401 on 1a2

Mini-prep

Researcher: Nishimura

Part: BBa_B0034 on 1a2

PEG Precipitation

Researcher: Nishimura

Part: BBa_B0034 on 1a2

Electrophoresis

Researcher: Nishimura

Part: BBa_B0034 on 1a2

Colony PCR

Researcher: Shibata, Nishimura, Kishida

Part: idt4

PCR (3 STEP)

Researcher: Shibata

Part: BBa_J04500

Transformation

Researcher: Kishida

Part: BBa_J04500 on 1c3

PEG Precipitation

Researcher: Shibata

Part: BBa_J04500 on 1c3

Electrophoresis

Researcher: Kishida

Part: idt4

Electrophoresis

Researcher: Shibata

Part: BBa_J04500

Digestion

Researcher: Shibata

Part: BBa_J04500 on 1c3

Electrophoresis

Researcher: Shibata

Part: BBa_J04500 on 1c3

Digestion

Researcher: Mimata, Shibata

Part: BBa_J04500 on 1c3

Ethanol Precipitation

Researcher: Mimata, Shibata

Part: BBa_J04500 on 1c3

Liquid Culture

Researcher: Mimata, Shibata

Part: BBa_J04500 - BBa_I13401 on 1c3, BBa_J04500 - idt7 on 1c3

PCR (2 STEP)

Researcher: Mimata, Shibata

Part: BBa_R0010, BBa_B0034

Ethanol Precipitation

Researcher: Mimata, Shibata

Part: BBa_J04500 on 1c3, BBa_R0010, BBa_B0034

2016/10/09

Mini-prep

Researcher: Nishimura, Hirao

Part: BBa_J04500 - idt7 on 1c3, BBa_J04500 on 1c3, BBa_J04500 - BBa_I13401 on 1c3, BBa_B0034 on 1a2

Electrophoresis

Researcher: Mimata

Part: BBa_J04500 - idt7 on 1c3, BBa_J04500 on 1c3, BBa_J04500 - BBa_I13401 on 1c3, BBa_B0034 on 1a2

Colony PCR

Researcher: Nishimura

Part: idt4

Digestion

Researcher: Mimata

Part: BBa_J04500 on 1c3

PEG Precipitation

Researcher: Mimata, Nishimura, Hirao

Part: BBa_J04500 on 1c3, BBa_B0034 on 1a2

Digestion

Researcher: Mimata

Part: BBa_J04500 on 1c3

PCR (2 STEP)

Researcher: Hirao, Kono

Part: idt6 on 1c3, idt7 on 1c3

Electrophoresis

Researcher: Hirao, Nishimura

Part: BBa_J04500 on 1c3, BBa_J04500, BBa_B0034

Electrophoresis

Researcher: Hirao

Part: idt4

Transformation

Researcher: Mimata, Shibata

Part: BBa_I715039 on 1c3, BBa_K741002 on 1c3, BBa_J04450 on 1c3

Electrophoresis

Researcher: Hirao, Kono

Part: idt6, idt7

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_R0010 - BBa_B0034

Electrophoresis

Researcher: Kono

Part: idt6, idt7

Gel Extract

Researcher: Kono

Part: idt6, idt7

Liquid Culture

Researcher: Shibata

Part: idt4 on 1c3

Electrophoresis

Researcher: Fujita, Mimata, Kono

Part: idt6, idt7, BBa_R0010 - BBa_B0034

2016/10/10

Mini-prep

Researcher: Fujita

Part: idt4 on 1c3

PEG Precipitation

Researcher: Fujita

Part: idt4 on 1c3

Electrophoresis

Researcher: Nishimura

Part: idt4 on 1c3

Digestion

Researcher: Fujita

Part: idt4 on 1c3

Electrophoresis

Researcher: Fujita

Part: idt4 on 1c3

Digestion

Researcher: Nishimura

Part: BBa_R0010, BBa_B0034

Transformation

Researcher: Nishimura

Part: BBa_K741002 on 1c3, BBa_J04450 on 1c3

Electrophoresis

Researcher: Nishimura, Shibata

Part: BBa_R0010, BBa_B0034

Electrophoresis

Researcher: Nishimura, Shibata

Part: BBa_R0010

Gel Extract

Researcher: Nishimura, Shibata

Part: BBa_R0010

Ethanol Precipitation

Researcher: Nishimura

Part: BBa_B0034

Electrophoresis

Researcher: Hirao

Part: BBa_R0010, BBa_B0034

Digestion

Researcher: Shibata, Hirao

Part: BBa_R0010 on 1c3, BBa_B0034 on 1a2

Ligation

Researcher: Nishimura

Part: BBa_R0010 & BBa_B0034 on 1c3

Transformation

Researcher: Nishimura

Part: BBa_R0010 - BBa_B0034 on 1a2

2016/10/11

PCR (2 STEP)

Researcher: Nishimura

Part: BBa_R0010 - BBa_B0034

PEG Precipitation

Researcher: Nishimura

Part: BBa_R0010 on 1c3

Liquid Culture

Researcher: Nishimura

Part: BBa_R0010 on 1c3

Transformation

Researcher: Nishimura

Part: BBa_K880005 on 1c3

Ligation

Researcher: Shibata

Part: BBa_R0010 & BBa_B0034 on 1a2

Transformation

Researcher: Fujita

Part: BBa_R0010 - BBa_B0034 on 1a2

Mini-prep

Researcher: Mimata

Part: BBa_R0010 on 1c3, BBa_B0034 on 1c3

Electrophoresis

Researcher: Mimata

Part: BBa_R0010 - BBa_B0034, BBa_R0010 on 1c3, BBa_B0034 on 1a2

Transformation

Researcher: Mimata

Part: BBa_K741002 on 1c3

PEG Precipitation

Researcher: Mimata, Kishida

Part: BBa_R0010 on 1c3, BBa_B0034 on 1a2

Electrophoresis

Researcher: Mimata, Kono, Kishida

Part: BBa_R0010 on 1c3, BBa_B0034 on 1a2

2016/10/12

Liquid Culture

Researcher: Shibata

Part: BBa_K741002 on 1c3

Colony PCR

Researcher: Shibata, Mimata, Kishida

Part: idt4

Transformation

Researcher: Nishimura

Part: pet

Liquid Culture

Researcher: Shibata, Mimata

Part: idt4 on 1c3, idt5 on 1c3

Electrophoresis

Researcher: Kishimoto

Part: idt4

2016/10/13

PCR (3 STEP)

Researcher: Nishimura

Part: idt5

Mini-prep

Researcher: Nishimura, Kishimoto

Part: idt4 on 1c3

Electrophoresis

Researcher: Shibata

Part: idt5 on 1a2, BBa_E0040

PEG Precipitation

Researcher: Shibata, Nishimura

Part: BBa_K741002 on 1c3

Electrophoresis

Researcher: Nishimura, Hirao

Part: idt5 on 1a2, BBa_E0040

Gel Extract

Researcher: Nishimura, Hirao

Part: idt5 on 1a2, BBa_E0040

Mini-prep

Researcher: Kono, Nishimura

Part: idt5 on 1a2, idt5 on 1c3

PCR (3 STEP)

Researcher: Nishimura

Part: idt5 on 1a2

Transformation

Researcher: Nishimura

Part: BBa_E0040 on 1a2, BBa_J04450 on 1c3

Electrophoresis

Researcher: Kishida

Part: BBa_K741002 on 1c3

Liquid Culture

Researcher: Kishida

Part: pet

Electrophoresis

Researcher: Kishida, Kono

Part: idt5 on 1a2

2016/10/14

Digestion

Researcher: Nishumura

Part: idt5, idt6, idt7

Electrophoresis

Researcher: Nishimura

Part: idt5 on 1a2

Ethanol Precipitation

Researcher: Nishimura

Part: idt5

Electrophoresis

Researcher: Nishimura

Part: idt5 on 1a2, idt6, idt7

Mini-prep

Researcher: Kono, Mimata

Part: pet

Electrophoresis

Researcher: Kono, Mimata

Part: pet

PEG Precipitation

Researcher: Kono, Mimata

Part: pet

PCR (2 STEP)

Researcher: Mimata

Part: idt6, idt7

Electrophoresis

Researcher: Mimata

Part: pet, idt5, idt6, idt7

Ligation

Researcher: Fujita

Part: idt5 & idt6 on 1c3, idt5 & idt7 on 1c3

Digestion

Researcher: Mimata, Fujita, Shibata, Kono

Part: pet, idt6, idt7

Transformation

Researcher: Fujita

Part: idt5 - idt6 on 1c3, idt5 - idt7 on 1c3

Streaking (Single Colony Isolation)

Researcher: Kono

Part: BBa_E0040 on 1a2

Ethanol Precipitation

Researcher: Mimata, Fujita

Part: pet, idt6, idt7

Electrophoresis

Researcher: Mimata, Fujita

Part: pet, idt6, idt7, idt4 on 1c3

2016/10/15

2016/10/16

2016/10/17

2016/10/18

2016/10/19

@@ Line 6,355: / Line 6,355: @@
 </div>
 <!--begin footer-->
+</div>
 </div>

Difference between revisions of "Team:HokkaidoU Japan/Notebook"

Revision as of 13:56, 17 October 2016

Team:HokkaidoU Japan