Difference between revisions of "Team:Pretoria UP"

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<p>Recent improvements of photo-bioelectrochemical cells (PBEC), which harness electrons from photosynthesis to generate electricity, include synthetic attachment of chloroplast thylakoids to graphene electrodes. However, current attachment techniques require costly chemically synthesized linkers and PBECs are not yet efficient enough for industrial energy generation. In this project, DNA aptamers were designed and evaluated as low-cost biological linkers to tether plant photosystem II (PSII) complexes to graphene foam electrodes. This project aims to improve the attachment and orientation of the PSII complex to the graphene electrode for higher electron transfer efficiency, and serves as a prototype for the in planta expression of RNA aptamers for self-assembling thylakoid attachment.
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<p>As we all know fossil fuels are running out and we need to find alternative and green energy sources. We have found a solution! The solution lies in combining the thylakoid membrane of plants` chlorophyll to a graphene plate (a superconductive carbon plate). When sunlight shines on the thylakoid membrane free electrons are generated and these electrons can be harvested to generate electricity. The key is to bind the thylakoid membrane to the graphene plate (this becomes the anode). In order to bind the thylakoid membrane we have engineered the sequence of short DNA pieces, called aptamers. One end of the aptamer binds specifically to the electron producing protein in the thylakoid membrane and the other side to the graphene plate. When electrons are then produced by the thylakoid membrane, they travel through the aptamer and to the graphene plate. Laccases is attached to the cathode which acts as an electron acceptor. This creates a potential difference and so are electricity generated by plants!
 
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<p style="text-align: left"><a href="https://2016.igem.org/Team:Pretoria_UP/Description">Read more on our Project Description page.</a>
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<p style="text-align: center"><a href="https://2016.igem.org/Team:Pretoria_UP/Description">Read more on our Project Description page.</a>
 
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Revision as of 08:24, 18 October 2016

WATTS-APTAMER - PRETORIA_UP iGEM

WATTS-APTAMER - PRETORIA_UP iGEM

Synthetic laccases and DNA aptamers for thylakoid tethering in photo-bioelectrochemical cells

Human practices

During our Human Practices project we gained valuable knowledge from experts in the field. We held a synthetic biology workshop and symposium, and presented to 40 high school learners at our university's "UP with Science" day. We also used various platforms of social media to create awareness of both our project and the fun we had during it!


Collaboration

We collaborated with three different teams in the 2016 iGEM season. We created a documentary for team Aix-Marseille focusing on the socio-economic and political issues facing the current platinum sector, including the Marikana strikes. We assisted the MacQuarie iGEM team by making a graphical user interface on MatLab® showing how the concentration of various intermediates, including ALA, (Y-axis) changes over time (X-axis) in chlorophyll.

Click here to read more.

We would like to extend special thanks to the following organizations for their support:




WATTS-APTAMER - PRETORIA_UP iGEM