Difference between revisions of "Team:HokkaidoU Japan/Proof"

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{{HokkaidoU_Japan}}
 
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<div id="Project_Description"><img src="https://static.igem.org/mediawiki/2016/5/58/T--HokkaidoU_Japan--proof_of_concept.png" width="300px" height="90px" alt="Proof of concept"></div>
  
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<h3>★  ALERT! </h3>
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We conducted SDS-PAGE with
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Medals">gold medal criterion for proof of concept</a>. </p>
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(BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on （pSB1C3）of E.coli（DH5α）.
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At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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iGEM teams are great at making things work! We value teams not only doing an incredible job with theoretical models and experiments, but also in taking the first steps to make their project real.
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<h4> What should we do for our proof of concept? </h4>
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You can assemble a device from BioBricks and show it works. You could build some equipment if you're competing for the hardware award. You can create a working model of your software for the software award. Please note that this not an exhaustive list of activities you can do to fulfill the gold medal criterion. As always, your aim is to impress the judges!
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<table>
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  <tr align="center">
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    <td>Materials</td>
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    <td>Volume</td>
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  </tr>
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  <tr align="center">
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    <td>30％ Acrilmid</td>
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    <td>5 mL</td>
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    <td>Tris-Buffer</td>
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    <td>2.5 mL</td>
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    <td>SDS</td>
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    <td>100 &micro;L</td>
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    <td>APS</td>
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    <td>100 &micro;L</td>
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    <td>TEMED</td>
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    <td>5 &micro;L</td>
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    <td>DW</td>
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    <td>2.295 ｍL</td>
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    <td>Total</td>
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    <td>10 ｍL</td>
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</table>
  
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Revision as of 11:37, 18 October 2016

Team:HokkaidoU Japan - 2016.igem.org

 

Team:HokkaidoU Japan

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Proof of concept

We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on （pSB1C3）of E.coli（DH5α）. At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
Materials Volume
30％ Acrilmid 5 mL
Tris-Buffer 2.5 mL
SDS 100 µL
APS 100 µL
TEMED 5 µL
DW 2.295 ｍL
Total 10 ｍL