Difference between revisions of "Team:Wageningen UR/Notebook"
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<h3 style="color:#6d5d31; font-size:18px;">Optogenetic Kill Switch Model</h3> <!--Change text color here--> | <h3 style="color:#6d5d31; font-size:18px;">Optogenetic Kill Switch Model</h3> <!--Change text color here--> | ||
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Revision as of 15:48, 18 October 2016
Find the documentation of our research here.
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Protein Engineering
We wanted to engineer Cry3Aa to become toxic to Varroa destructor.
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In Vitro Assay
To provide an alternative for tests on live Varroa mites, we designed an assay that uses brush border membrane vesicles.
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Varroa isolates
We tried to find our own Varroa entomopathogen.
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Riboswitches
To detect the presence of Varroa mites in the beehive, we made a B12 and a guanine riboswitch.
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Population Dynamics
A quorum sensing system was characterized to regulate toxin production in BeeT.
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Optogenetic Kill Switch
The light-induced kill switch was one of our biocontainment methods.
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Modelling
How we modelled.
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Optogenetic Kill Switch Model
The light-induced kill switch was modeled with ODEs to understand the system in more detail.
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Chitinases
As a back-up, we wanted to use chitinases which are more harmful to Varroa destructor than honeybees.
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Cas9-based Kill Switch
The Cas9-based kill switch was one of our biocontainment methods.
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Toxin Scanner
We made a software tool for finding new Cry proteins toxic to Varroa destructor.
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Metabolic Modelling
We wanted to use a metabolic model to see the effect of our BeeT distribution method.
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Testing E. coli survival
Following the results of the Metabolic Model, we needed more data.
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Human Outreach
We implemented our outreach into our research as much as we could. Find the details here!
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Toggle Switch
We designed a toggle switch that could coordinate mites sensing, BT's toxin production and light sensing with fast responsiveness.
