Difference between revisions of "Team:TAS Taipei/Attributions"

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<a href="https://2016.igem.org/Team:TAS_Taipei/Description"><h4 class="dropdown-toggle disabled" data-toggle="dropdown"><b>PROJECT</b></h4></a>
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<a href="https://2016.igem.org/Team:TAS_Taipei/Background"><h4 class="dropdown-toggle disabled" data-toggle="dropdown"><b>PROJECT</b></h4></a>
 
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<h5><a href="https://2016.igem.org/Team:TAS_Taipei/Description">Background</a></h5>
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<a href="https://2016.igem.org/Team:TAS_Taipei/Safety"><h4 class='dropdown-toggle disabled' data-toggle="dropdown"><b>BIOSAFETY</b></h4></a>
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<a href="https://2016.igem.org/Team:TAS_Taipei/Safety"><h4 class='dropdown-toggle disabled' data-toggle="dropdown"><b>SAFETY</b></h4></a>
 
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<li><a href="#lensmodel">Lens Cataract Model</a></li>
+
<h1>Attributions</h1>
−
                        <ul>
+
</div>
−
                            <li> <a href="#LensPrevention">Prevention</a></li>
+
−
                            <li> <a href="#LensTreatment">Treatment</a></li>
+
−
                        </ul>
+
−
<li><a href="#construct">Construct</a></li>
+
−
                        <ul>
+
−
                            <li> <a href="#ConstructPrevention">Prevention</a></li>
+
−
                            <li> <a href="#ConstructTreatment">Treatment</a></li>
+
−
                        </ul>
+
−
                        <li><a href="#prototype">Delivery Prototype</a></li>
+
−
                        <ul>
+
−
                            <li> <a href="#step1">Purification</a></li>
+
−
                            <li> <a href="#step2">Nanoparticle</a></li>
+
−
                            <li> <a href="#step2i">Encapsulation</a></li>
+
−
                            <li> <a href="#step2ii">Release</a></li>
+
−
                            <li> <a href="#step3">Application</a></li>
+
−
                        </ul>
+
−
</ul>
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</div>
 
</div>
  
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</div>
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<div class="row" style = "font-size:16px">
 +
<div class="col-sm-12"><br>
 +
<ul>
 +
<li>
 +
All lab work was conducted at Taipei American School Sandy R. Puckett Memorial Research Laboratory by team members of the 2016 TAS-Taipei iGEM team, unless stated otherwise
 +
</li>
 +
<br>
 +
<li>
 +
National Yang Ming University - NYMU_Taipei team (<a href = "https://2015.igem.org/Team:TAS_Taipei/Collaborations">click to see collaboration page</a>)
 +
<ul><li>
 +
Consultation for troubleshooting our cloning procedure
 +
</li>
 +
                                <li>Ran summer camp to overview entire iGEm process from start to finish</li>
 +
                                <li>Ran nanoparticle calculator to give advice and troubleshoot problems and misunderstandings</li></ul>
  
 +
</li>
 +
                            <li>ASIJ - ASIJ_Tokyo team
 +
                                <ul>
 +
                                    <li>Testing our cataract treatment and prevention calculator and providing user feedback</li>
 +
                                </ul>
 +
                            </li>
 +
                            <li>Dr. Jason Cyster - Professor at UCSF University Department of Microbiology and Immunology
 +
                                <ul>
 +
                                    <li>Advice on working with 25HC and exploring its properties in terms of cataracts treatment</li>
 +
                                </ul>
 +
                            </li>
 +
                            <li>Dr. Tsu Chieh Cheng - Optomologist at Chang Cheng Eye Hospital
 +
                                <ul>
 +
                                    <li>Information on challenges with current drug delivery systems and cataracts surgery</li>
 +
                                    <li>Feedback on our prototype plan</li>
 +
                                </ul>
 +
                            </li>
 +
                            <li>Dr. Wei-Chu Wu - Retina Specialist, Associate Professor at Chang Gung Memorial Hospital
 +
 +
                                <ul><li>Information on current cataracts treatments and post operational complications</li></ul>
 +
                            </li>
 +
                            <li>Dr. Li Guo Min - Veterinarian at Nicholas Animal Hospital
 +
                                <ul><li>Information on cataract development in animals and current treatments, such as the Ocluvet eye drops</li></ul>
 +
 +
                            </li>
 +
                            <li>Dr. Jason Gestwicki - Professor at UCSF School of Medicine
 +
                                <ul><li>Advice on 25HC regarding protein aggression and molecular interaction</li></ul></li>
 +
                            <li>Dr. Eric (Ricky) P. Lee -  Senior member of Technical Staff at Maxim Integrated
 +
                                <ul><li>Assistance with troubleshooting problems encountered during nanoparticle synthesis and protein encapsulation
 +
                                    </li>
 +
                                </ul>
 +
                            </li>
 +
                            <li>Mrs. Michelle Bruce - Economics Teacher at Taipei American School
 +
                                <ul><li>Reviewing and providing feedback on our business plan</li></ul>
 +
                            </li>
 +
                            <li>Mr. Alexander kaufman - Former intern at RA Capital
 +
                                <ul><li>Feedback on our business plan</li></ul>
 +
                            </li>
 +
                            <li>Dr. Catriona Moran and Mrs. Sharon Clapper
 +
                                <ul><li>Sharing their experiences as cataracts patients and feedback on our final product</li></ul>
 +
                            </li>
 +
                            <li>Mission Biotech
 +
                                <ul><li>Internal cutting sites removal for GSR and CH25H</li></ul>
 +
 +
                            </li>
 +
                            <li>Integrated DNA Technologies
 +
                                <ul><li>CH25H gene fragment, CRYAB gene fragment, GFP full construct, CH25H full construct
 +
</li></ul>
 +
                            </li>
 +
                            <li>Tri-I biotech
 +
                                <ul>
 +
                                <li>Sequencing and primers synthesis</li>
 +
                                    <li>Primer synthesis to remove stop codon (every gene we ordered)</li>
 +
                                </ul>
 +
                            </li>
 +
                            <li>
 +
                                <ul><li>Origene </li><li>cDNA for CRYAA and GSR</li>
 +
</ul>
 +
                            </li>
 +
<br>
 +
 +
<br>
 +
 +
<br>
 +
<li>
 +
People involved in our bioethics panel
 +
<ul>
 +
                                    <li>Mr. Steve Anderson</li>
 +
                                    <li>Ms. Anisha Vinod</li>
 +
<li>Dr. Nathaniel Smith</li>
 +
<li>Dr. Evonne Vidal</li>
 +
                                    <li>Mr. Brandon Maguire</li>
 +
<li>Dr. Annemarie Costello</li>
 +
 +
 +
</ul>
 +
</li>
 +
<br>
 +
</ul>
 +
 +
 +
<br>
 +
 +
Some parts of this wiki have been derived from the 2014 and 2015 TAS_Taipei wiki, which was designed entirely by the 2014 and 2015 teams. All other components were designed by students of the 2016 team.
 +
<br>
 +
<br>
 +
 +
<ul>
 +
<li>
 +
DR. SHARON HENNESSY, TAS SUPERINTENDENT
 +
</li>
 +
<li>
 +
DR. RICHARD HARTZELL, TAS UPPER SCHOOL PRINCIPAL
 +
</li>
 +
<li>
 +
FRIENDS OF TAS
 +
</li>
 +
</ul>
 +
<br>
 +
<p>A huge thank you to our collaborators and sponsors.</p>
 +
<br>
 +
</div>
 +
</div>
 +
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<div class="row">
 +
<div class="col-sm-4">
 +
<img src="https://static.igem.org/mediawiki/2015/b/b0/Attr_yangMing.gif" alt="">
 +
</div>
 +
<div class="col-sm-4">
 +
<img src="https://static.igem.org/mediawiki/2015/c/c7/Attr_biobuilder.png" alt="">
 +
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<div class="col-sm-4">
 +
<img src="https://static.igem.org/mediawiki/2015/f/f7/Attr_geneaid.png" alt="">
 +
</div>
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<div class="col-sm-4">
 +
<img src="https://static.igem.org/mediawiki/2015/7/78/Attr_trii.jpg" alt="">
 +
</div>
 +
<div class="col-sm-4">
 +
<img src="https://static.igem.org/mediawiki/2015/3/3e/Attr_mission.gif" alt="">
 +
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 +
<div class="col-sm-4">
 +
<img src="https://static.igem.org/mediawiki/2015/b/ba/Attr_sino.png" alt="">
 +
</div>
 +
 +
</div>
 +
 +
</div>
  
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<h1>Attributions</h1>
 
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        <h2 id = 'construct'>Research</h2>
 
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                                    <br>
 
−
                                <h3>Professional Help</h3>
 
−
                                <p>
 
−
                                    In order to improve how we develop our prototype we needed to get in contact with experts in the fields of eye surgery and cataracts research. Our first step was to contact eye doctors to discuss any problems associated with current cataracts treatment. Finally, while developing our project we realized that cataracts is a major issue in pets and other animals as well as people. As a result, we contacted local veterinarians to discuss what pet owners do when their pets contract cataracts. As our project developed, we needed more specific information regarding our genes of interest, cataracts development, and our delivery mechanism. We contacted scientists doing research similar to our own to get their opinion on our projects progress.
 
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                                    <h4>Contact with Eye Doctors</h4>
 
−
                                    <p>
 
−
                                        Eye doctors are on the front lines, delivering cataracts surgery, some privately and others for charity in organizations such as the Himalayan Cataracts Projects. We contacted local Taiwanese Eye Doctors to ask them about cataracts surgery. Here is a list of the doctors we contacted along with the information they provided:
 
−
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                                        <br>
 
−
                                    <p>
 
−
                                        Dr. Wei-Chi Wu is a retina specialist and an associate professor at Chang Gung memorial hospital in Taiwan. According to Dr. Wu current cataracts surgery methods are efficient and effective, but are not without their issues. Besides the issue of price, there can also be several different post-surgery complications such as infection, hemorrhaging, or secondary glaucoma. In regards to our project, he said one of the biggest issues we would face is non-invasive delivery. Currently, injections and incisions are the only methods for delivery because all current potential methods of noninvasive delivery either lack efficiency or induce with side effects.
 
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                                <figure class = "col-sm-6">
 
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        <img src="https://static.igem.org/mediawiki/2016/6/67/T--TAS_Taipei--GSR_Construct_Experimental.jpg">
 
−
                                    <figcaption class='darkblue'><b>Figure X. </b>Full Construct.</figcaption>
 
−
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<h3>Citations</h3>
 
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        <br> <br>  <br>  <br>  <br>  <br>  <br>  <br>           
 
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         var NineLOCSnum = Math.round(nowRadius*6/9*10);
 
         var NineLOCSnum = Math.round(nowRadius*6/9*10);
 
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         /**if (NineLOCSnum == 15) $("#slideoutco").fadeOut(400);
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         if (NineLOCSnum > 20) switchMessage("RED","<b>Cataracts</b> are creeping back again! Click the PREVENTION eyedrop to add GSR into your eyes!", currentColor()=="BLUE");*/
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         if (NineLOCSnum > 20) switchMessage("RED","<b>Cataracts</b> are creeping back again! Click the PREVENTION eye drop to add GSR into your eyes!", currentColor()=="BLUE");*/
 
         if (LOCSnum > 6) {LOCSnum = "6+"; $('#LOCS').text(LOCSnum+"");}
 
         if (LOCSnum > 6) {LOCSnum = "6+"; $('#LOCS').text(LOCSnum+"");}
 
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                 <strong>The tutorial is disabled.</strong> &#160; &#160; &#160; &#160; &#160;<a href="https://2016.igem.org/Team:TAS_Taipei/Wiki_Standard_Pages#Animation"><button  type="button" class="btn btn-danger btn-sm">?</button> </a><span style="font-size:14px"><br>Turn off prevention eyedrops to activate the animation. For a full tutorial, click the question mark. </span></p>
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                 <strong>Zoom out to see animation.</strong> &#160; &#160; &#160; &#160; &#160;<a href="https://2016.igem.org/Team:TAS_Taipei/Wiki_Standard_Pages#Animation"><button  type="button" class="btn btn-danger btn-sm">?</button> </a><span style="font-size:14px"><br>Your screen resolution is too low unless you zoom out </span></p>
 
              
 
              
 
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Revision as of 18:56, 18 October 2016

Entrepreneurship - TAS Taipei iGEM Wiki




C◎UNTERACTS

Attributions


  • All lab work was conducted at Taipei American School Sandy R. Puckett Memorial Research Laboratory by team members of the 2016 TAS-Taipei iGEM team, unless stated otherwise

  • National Yang Ming University - NYMU_Taipei team (click to see collaboration page)
    • Consultation for troubleshooting our cloning procedure
    • Ran summer camp to overview entire iGEm process from start to finish
    • Ran nanoparticle calculator to give advice and troubleshoot problems and misunderstandings
  • ASIJ - ASIJ_Tokyo team
    • Testing our cataract treatment and prevention calculator and providing user feedback
  • Dr. Jason Cyster - Professor at UCSF University Department of Microbiology and Immunology
    • Advice on working with 25HC and exploring its properties in terms of cataracts treatment
  • Dr. Tsu Chieh Cheng - Optomologist at Chang Cheng Eye Hospital
    • Information on challenges with current drug delivery systems and cataracts surgery
    • Feedback on our prototype plan
  • Dr. Wei-Chu Wu - Retina Specialist, Associate Professor at Chang Gung Memorial Hospital
    • Information on current cataracts treatments and post operational complications
  • Dr. Li Guo Min - Veterinarian at Nicholas Animal Hospital
    • Information on cataract development in animals and current treatments, such as the Ocluvet eye drops
  • Dr. Jason Gestwicki - Professor at UCSF School of Medicine
    • Advice on 25HC regarding protein aggression and molecular interaction
  • Dr. Eric (Ricky) P. Lee - Senior member of Technical Staff at Maxim Integrated
    • Assistance with troubleshooting problems encountered during nanoparticle synthesis and protein encapsulation
  • Mrs. Michelle Bruce - Economics Teacher at Taipei American School
    • Reviewing and providing feedback on our business plan
  • Mr. Alexander kaufman - Former intern at RA Capital
    • Feedback on our business plan
  • Dr. Catriona Moran and Mrs. Sharon Clapper
    • Sharing their experiences as cataracts patients and feedback on our final product
  • Mission Biotech
    • Internal cutting sites removal for GSR and CH25H
  • Integrated DNA Technologies
    • CH25H gene fragment, CRYAB gene fragment, GFP full construct, CH25H full construct
  • Tri-I biotech
    • Sequencing and primers synthesis
    • Primer synthesis to remove stop codon (every gene we ordered)
    • Origene
    • cDNA for CRYAA and GSR



  • People involved in our bioethics panel
    • Mr. Steve Anderson
    • Ms. Anisha Vinod
    • Dr. Nathaniel Smith
    • Dr. Evonne Vidal
    • Mr. Brandon Maguire
    • Dr. Annemarie Costello


Some parts of this wiki have been derived from the 2014 and 2015 TAS_Taipei wiki, which was designed entirely by the 2014 and 2015 teams. All other components were designed by students of the 2016 team.

  • DR. SHARON HENNESSY, TAS SUPERINTENDENT
  • DR. RICHARD HARTZELL, TAS UPPER SCHOOL PRINCIPAL
  • FRIENDS OF TAS

A huge thank you to our collaborators and sponsors.





Prevention

GSR Eyedrop

Treatment

25HC Eyedrop

LOCS: 0      


Eyedrops




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