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+ | <h1 style="text-align:center; font-size: 50px;"> <font face= "Poiret One">Code for Mathematica</h2> | ||
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+ | <p style="text-align:center; font-size:20px;"><font face="verdana">To access a <i>.txt</i> file of the Mathematica code, click <a href="https://static.igem.org/mediawiki/2016/5/53/Modeling.txt" target="_blank"><u>here</u></a>.</p> | ||
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<h1 style="text-align:center; font-size: 50px;"> <font face= "Poiret One">Conclusion</h2> | <h1 style="text-align:center; font-size: 50px;"> <font face= "Poiret One">Conclusion</h2> | ||
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− | <p style="text-align:center; font-size:20px;"><font face="verdana">In order to confirm that our mathematical model was, indeed, functional, we confirmed the patterns that were to be predicted in the model. For example, a higher | + | <p style="text-align:center; font-size:20px;"><font face="verdana">In order to confirm that our mathematical model was, indeed, functional, we confirmed the patterns that were to be predicted in the model. For example, a higher <i>kd</i> value would lead to less aptamer-protein complex formation. As expected, this pattern was seen in our model, as a 2-fold decrease in the <i>kd</i> value, generally led to a greater than 10-fold decrease in the protein-aptamer complex that was expected to form.</p> |
<p style="text-align:center; font-size:20px;"><font face="verdana">Our mathematical model was also able to show that lower concentrations of the protein with constant concentrations of aptamer 1, aptamer 2, and the connector would lead to lower overall complex formations. In other words, when the protein is missing in the system, it is very unlikely for unspecific reactions to take place, and thereby give a false positive signal. As the protein concentration was gradually increased, the number of aptamer-protein complexes increased at an exponential rate, indicating the sensitivity of the aptamer-protein complex. In other words, only a small amount of the target protein is needed for the complex to form. </p> | <p style="text-align:center; font-size:20px;"><font face="verdana">Our mathematical model was also able to show that lower concentrations of the protein with constant concentrations of aptamer 1, aptamer 2, and the connector would lead to lower overall complex formations. In other words, when the protein is missing in the system, it is very unlikely for unspecific reactions to take place, and thereby give a false positive signal. As the protein concentration was gradually increased, the number of aptamer-protein complexes increased at an exponential rate, indicating the sensitivity of the aptamer-protein complex. In other words, only a small amount of the target protein is needed for the complex to form. </p> |
Revision as of 21:54, 18 October 2016