Difference between revisions of "Team:UBonn HBRS/Description/Notebook/2016"
| Line 1: | Line 1: | ||
{{UBonn_HBRS/header|title=Lab Notebook 2016|Project=active}} | {{UBonn_HBRS/header|title=Lab Notebook 2016|Project=active}} | ||
| − | 10KW (7.3-13.3) | + | ==10KW (7.3-13.3)== |
transformation of genes bought at genescript: XynA, XynB, LipA, EstC2, CpCel9, CpCel5c, EngB, CelA, BsCel5, Bpul | transformation of genes bought at genescript: XynA, XynB, LipA, EstC2, CpCel9, CpCel5c, EngB, CelA, BsCel5, Bpul | ||
| Line 11: | Line 11: | ||
restriction analysis of the genes. | restriction analysis of the genes. | ||
| − | 11KW (14.3-20.3) | + | ==11KW (14.3-20.3)== |
Repetion of restriction analysis from the genes KW10 and gel clean ups of the linkers. | Repetion of restriction analysis from the genes KW10 and gel clean ups of the linkers. | ||
| − | 12KW (21.3-27.3) | + | ==12KW (21.3-27.3)== |
repetition of restriction analysis of all genes and gel clean ups of the genes. | repetition of restriction analysis of all genes and gel clean ups of the genes. | ||
| − | 13KW (28.3-3.4) | + | ==13KW (28.3-3.4)== |
Restrictions and gel clean ups of all genes genes for a new gel clean up, because the ones of KW13 did not works. Ligation of genes from the gel clean ups into C3 and inoculation of cultures and minipreps. | Restrictions and gel clean ups of all genes genes for a new gel clean up, because the ones of KW13 did not works. Ligation of genes from the gel clean ups into C3 and inoculation of cultures and minipreps. | ||
(1.04.16 :) | (1.04.16 :) | ||
-> successful ligation of | -> successful ligation of | ||
| − | XynA | + | *XynA |
| − | CelA | + | *CelA |
| − | LipA | + | *LipA |
| − | Bpul | + | *Bpul |
| − | CpCel5c | + | *CpCel5c |
| − | BsCel5 | + | *BsCel5 |
| − | EstC2 | + | *EstC2 |
| − | EngB in C3 | + | *EngB in C3 |
| − | nprb + CpCel9 | + | *nprb + CpCel9 |
| − | nprb + BsCel5 in C3 | + | *nprb + BsCel5 in C3 |
| − | 21KW (23.5-29.5) | + | ==21KW (23.5-29.5)== |
Restriction analysis of genes from the 13KW and mini prep from samples of the 13KW. | Restriction analysis of genes from the 13KW and mini prep from samples of the 13KW. | ||
| Line 42: | Line 42: | ||
successful ligation of: | successful ligation of: | ||
| − | nprb and sacB in C3 | + | *nprb and sacB in C3 |
| − | nprb + EstC2 in C3 | + | *nprb + EstC2 in C3 |
| − | 22KW (30.6-5.6) | + | ==22KW (30.6-5.6)== |
Restriction analysis of samples of the 21KW | Restriction analysis of samples of the 21KW | ||
| − | 23KW (6.6-12.6) | + | ==23KW (6.6-12.6)== |
Inoculation of all genes from glycerol stocks and miniprep. Restriction analysis and gel clean up of the genes, ligation of the missing genes with C3. | Inoculation of all genes from glycerol stocks and miniprep. Restriction analysis and gel clean up of the genes, ligation of the missing genes with C3. | ||
| − | 24KW (13.6-19.6) | + | ==24KW (13.6-19.6)== |
Restriction of bbMCS and gel clean ups. | Restriction of bbMCS and gel clean ups. | ||
| Line 61: | Line 61: | ||
Minipreps of all of the samples. | Minipreps of all of the samples. | ||
| − | 25KW (20.6-26.6) | + | ==25KW (20.6-26.6)== |
Restriction analysis of the constructs | Restriction analysis of the constructs | ||
Repetition of ligation of genes in C5 and transformation and preps. | Repetition of ligation of genes in C5 and transformation and preps. | ||
| − | 26KW (27.6 - 3.7) | + | ==26KW (27.6 - 3.7)== |
Restriction analysis of the mini preps. | Restriction analysis of the mini preps. | ||
| − | 28KW (11.7-7.17) | + | ==28KW (11.7-7.17)== |
Repetion of Ligations of genes and C5 | Repetion of Ligations of genes and C5 | ||
| − | 31KW (1.8-7.8) | + | ==31KW (1.8-7.8)== |
Ligation of Fragment synthesized at IDT and C3 | Ligation of Fragment synthesized at IDT and C3 | ||
| Line 78: | Line 78: | ||
transformation, and inoculation of liquid cultures of the above. | transformation, and inoculation of liquid cultures of the above. | ||
| − | 32KW (8.8 - 14.8) | + | ==32KW (8.8 - 14.8)== |
Ligation of IDT and C3 | Ligation of IDT and C3 | ||
| Line 87: | Line 87: | ||
Transformation of these samples | Transformation of these samples | ||
| − | 33KW (15.8 - 21.8) | + | ==33KW (15.8 - 21.8)== |
Inoculation and miniprep | Inoculation and miniprep | ||
| Line 97: | Line 97: | ||
mini preps | mini preps | ||
| − | 34KW (22.8 - 28.8) | + | ==34KW (22.8 - 28.8)== |
Restrictions of mini prep and repetition of mini prep | Restrictions of mini prep and repetition of mini prep | ||
{{UBonn_HBRS/footer}} | {{UBonn_HBRS/footer}} | ||
Revision as of 22:19, 18 October 2016
Lab Notebook 2016
Contents
10KW (7.3-13.3)
transformation of genes bought at genescript: XynA, XynB, LipA, EstC2, CpCel9, CpCel5c, EngB, CelA, BsCel5, Bpul and Linkers: nprb, SacB inoculation of liquid cultures and restriction Analysi of linkers (SacB and nprb).
repetition of the transformation for XynB, EngB and linkers. Subsequently inoculation and miniprep of the samples. Preparation of glycerol stock.
restriction analysis of the genes.
11KW (14.3-20.3)
Repetion of restriction analysis from the genes KW10 and gel clean ups of the linkers.
12KW (21.3-27.3)
repetition of restriction analysis of all genes and gel clean ups of the genes.
13KW (28.3-3.4)
Restrictions and gel clean ups of all genes genes for a new gel clean up, because the ones of KW13 did not works. Ligation of genes from the gel clean ups into C3 and inoculation of cultures and minipreps. (1.04.16 :) -> successful ligation of
- XynA
- CelA
- LipA
- Bpul
- CpCel5c
- BsCel5
- EstC2
- EngB in C3
- nprb + CpCel9
- nprb + BsCel5 in C3
21KW (23.5-29.5)
Restriction analysis of genes from the 13KW and mini prep from samples of the 13KW. (28.05.16) successful ligation of:
- nprb and sacB in C3
- nprb + EstC2 in C3
22KW (30.6-5.6)
Restriction analysis of samples of the 21KW
23KW (6.6-12.6)
Inoculation of all genes from glycerol stocks and miniprep. Restriction analysis and gel clean up of the genes, ligation of the missing genes with C3.
24KW (13.6-19.6)
Restriction of bbMCS and gel clean ups. Ligations of genes in C5 and C3 Ligation of genes and tags in C5. Minipreps of all of the samples.
25KW (20.6-26.6)
Restriction analysis of the constructs Repetition of ligation of genes in C5 and transformation and preps.
26KW (27.6 - 3.7)
Restriction analysis of the mini preps.
28KW (11.7-7.17)
Repetion of Ligations of genes and C5
31KW (1.8-7.8)
Ligation of Fragment synthesized at IDT and C3 Ligation of genes in C3 and genes + tags + C5 transformation, and inoculation of liquid cultures of the above.
32KW (8.8 - 14.8)
Ligation of IDT and C3 Transformation and inoculation. Minipreps of samples and restriction analysis
Ligations of genes plus tags in bb_MCS Transformation of these samples
33KW (15.8 - 21.8)
Inoculation and miniprep Repetion of ligation of IDT and C3 Repetion of ligation of bb + nprb + genes Midi prep of SacB, bb and nprb Ligation of Genes + C5 and genes plus tags in C5. Transformation and inoculation of the samples. mini preps
34KW (22.8 - 28.8)
Restrictions of mini prep and repetition of mini prep
