Revision as of 23:33, 18 October 2016

Lab Notebook 2016

10KW (7.3-13.3)

transformation of genes bought at genescript: XynA, XynB, LipA, EstC2, CpCel9, CpCel5c, EngB, CelA, BsCel5, Bpul and Linkers: nprb, SacB inoculation of liquid cultures and restriction Analysi of linkers (SacB and nprb).

repetition of the transformation for XynB, EngB and linkers. Subsequently inoculation and miniprep of the samples. Preparation of glycerol stock.

restriction analysis of the genes.

11KW (14.3-20.3)

Repetion of restriction analysis from the genes KW10 and gel clean ups of the linkers.

Deinking:

Measurement of inkjet absorbance spectrum
filtration-based method

12KW (21.3-27.3)

repetition of restriction analysis of all genes and gel clean ups of the genes.

13KW (28.3-3.4)

Restrictions and gel clean ups of all genes genes for a new gel clean up, because the ones of KW13 did not works. Ligation of genes from the gel clean ups into C3 and inoculation of cultures and minipreps. (1.04.16 :) -> successful ligation of

XynA
CelA
LipA
Bpul
CpCel5c
BsCel5
EstC2
EngB in C3

nprb + CpCel9
nprb + BsCel5 in C3

21KW (23.5-29.5)

Restriction analysis of genes from the 13KW and mini prep from samples of the 13KW. (28.05.16) successful ligation of:

nprb and sacB in C3

nprb + EstC2 in C3

22KW (30.6-5.6)

Restriction analysis of samples of the 21KW

23KW (6.6-12.6)

Inoculation of all genes from glycerol stocks and miniprep. Restriction analysis and gel clean up of the genes, ligation of the missing genes with C3.

24KW (13.6-19.6)

Restriction of bbMCS and gel clean ups. Ligations of genes in C5 and C3 Ligation of genes and tags in C5. Minipreps of all of the samples.

25KW (20.6-26.6)

Restriction analysis of the constructs Repetition of ligation of genes in C5 and transformation and preps.

26KW (27.6 - 3.7)

Restriction analysis of the mini preps.

28KW (11.7-7.17)

Repetion of Ligations of genes and C5

31KW (1.8-7.8)

Ligation of Fragment synthesized at IDT and C3 Ligation of genes in C3 and genes + tags + C5 transformation, and inoculation of liquid cultures of the above.

32KW (8.8 - 14.8)

Ligation of IDT and C3 Transformation and inoculation. Minipreps of samples and restriction analysis

Ligations of genes plus tags in bb_MCS Transformation of these samples

33KW (15.8 - 21.8)

Inoculation and miniprep Repetion of ligation of IDT and C3 Repetion of ligation of bb + nprb + genes Midi prep of SacB, bb and nprb Ligation of Genes + C5 and genes plus tags in C5. Transformation and inoculation of the samples. mini preps

34KW (22.8 - 28.8)

Restrictions of mini prep and repetition of mini prep

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