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− | == | + | == Scientific supervision == |
− | We | + | We are very grateful to '''Prof. Dr. Günther Mayer''' and '''Prof. Dr. Michael Famulok''' of University Bonn for supervising this project. |
− | + | We would also like to thank '''Prof. Dr. Klaus Lehmann''' of HBRS for his resourceful and unlimited support. | |
− | + | ||
− | + | ||
− | We thank | + | |
== Lab support == | == Lab support == | ||
− | + | Our dearest thanks go to our Instructor '''Ignacio Geraci''' and all the other '''PhDs and PhD students of <html><a href="http://www.limes-institut-bonn.de/forschung/arbeitsgruppen/unit-4/abteilung-mayer/mitarbeiter/labormitarbeiter/" target="_blank"></html>Professor Mayer’s laboratory<html></a></html>''', especially '''Christian Renzl''', for their help and patience. | |
<html><img src="//2016.igem.org/wiki/images/5/55/T--UBonn_HBRS--heart.svg" alt="Heart" class="col-xs-4 col-md-2" style="float:none"/></html> | <html><img src="//2016.igem.org/wiki/images/5/55/T--UBonn_HBRS--heart.svg" alt="Heart" class="col-xs-4 col-md-2" style="float:none"/></html> | ||
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== Project advisor support == | == Project advisor support == | ||
− | * '''Benedikt HÖLBLING''' <br />took care of the team’s finances and | + | * '''Benedikt HÖLBLING''' <br />took care of the team’s finances, initiated and organized the team's science slams. |
* '''BÙI Hoàng Duy''' <br />gave advices to the project. | * '''BÙI Hoàng Duy''' <br />gave advices to the project. | ||
− | * '''Niklas Arndt SCHMACKE''' <br />concerned himself with the theoretical and practical introduction of new team members | + | * '''Niklas Arndt SCHMACKE''' <br />Was involved in the development of the project idea, concerned himself with the theoretical and practical introduction of new team members and served as a remote advisor from Switzerland for a large part of the project. |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
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− | + | ||
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=== B. subtilis === | === B. subtilis === | ||
− | * '''Ariana Natalie KLAPPERT''' <br /> | + | * '''Ariana Natalie KLAPPERT''' <br />optimzied PAGE gel analysis and protein expression. |
* '''Jonas GOCKEL''' <br />prepared competent bacteria, tested transformation protocols and established the Bacillus Subtilis collaboration with Freiburg. | * '''Jonas GOCKEL''' <br />prepared competent bacteria, tested transformation protocols and established the Bacillus Subtilis collaboration with Freiburg. | ||
Line 47: | Line 34: | ||
=== Cloning === | === Cloning === | ||
− | * '''Caroline FANDREY''' <br />planned and executed restriction of genes, | + | * '''Caroline FANDREY''' <br />planned and executed restriction and ligation of genes, secretion tags and backbones and their transformation into E. coli, and validated the results. |
− | * '''Ariana Natalie KLAPPERT''' <br />supported the cloning group | + | * '''Ariana Natalie KLAPPERT''' <br />supported the cloning group during planning of restrictions, preparation of DNA, ligations and transformations. Ariana also worked on optimizing IPTG inducible protein expression in E. Coli. |
* '''BÙI Hoàng Duy''' <br />provided help in all tasks of cloning. | * '''BÙI Hoàng Duy''' <br />provided help in all tasks of cloning. | ||
Line 57: | Line 44: | ||
* '''Laura LÓPEZ NAVARRETE''' <br />collaborated in assembling DNA constructs of tags and genes in E. coli and prepared rapid ligase buffer. | * '''Laura LÓPEZ NAVARRETE''' <br />collaborated in assembling DNA constructs of tags and genes in E. coli and prepared rapid ligase buffer. | ||
− | * '''Michael TESKE''' <br />supported the team with restriction analysis, ligations, minipreps, transformations and | + | * '''Michael TESKE''' <br />supported the team with restriction analysis, ligations, minipreps, transformations and prepared rapid ligase buffer. |
− | * '''Sophia MÄDLER''' <br /> | + | * '''Sophia MÄDLER''' <br />assembled our working backbone, took care of introducing the working methods to new members, designed the pLac-RBS construct, performed analysis of sequencing results and planned experiments to assemble all constructs. |
* '''Triinu LINKGREIM''' <br />took part in backbone cloning. | * '''Triinu LINKGREIM''' <br />took part in backbone cloning. | ||
+ | |||
+ | * '''Niklas Arndt SCHMACKE''' <br />Designed the part sequences of nprb and sacB as well as the genes to be tested in deinking together with Max Schelski. | ||
=== Deinking === | === Deinking === | ||
− | * '''Gintarė MALININAITĖ''' <br /> | + | * '''Gintarė MALININAITĖ''' <br />was responsible for the deinking part of the project and took part in the design of the experiments. |
− | * '''Stefan KOTTE''' <br /> | + | * '''Stefan KOTTE''' <br />did a solvent analysis, established the grayscale analysis and refined the deinking process on the lab scale. |
=== Specific Enzyme Essays === | === Specific Enzyme Essays === | ||
− | * '''Triinu LINKGREIM''' <br /> | + | * '''Sophia MÄDLER''' <br />established all protocols to determine specific enzymatic activity of lipases, xylanases and endoglucanases in our lab. |
+ | |||
+ | * '''Triinu LINKGREIM''' <br />refined the enzyme activity assays and was responsible for measuring enzymatic activity in the project. | ||
Line 85: | Line 76: | ||
* '''BÙI Hoàng Duy''' <br />sponsoring delegate of the team. | * '''BÙI Hoàng Duy''' <br />sponsoring delegate of the team. | ||
− | * '''Gintarė MALININAITĖ''' <br /> | + | * '''Gintarė MALININAITĖ''' <br />responsible for the teams sponsoring undertakings, contacted paper companies and helped with science slam organization. |
− | * '''Jan Niklas HANSEN''' <br />co-organized Science Slam and provided contact with CAESAR institute. | + | * '''Jan Niklas HANSEN''' <br />co-organized Science Slam and provided contact with the CAESAR institute. |
− | * '''Laura LÓPEZ NAVARRETE''' <br />wrote an essay about biosafety | + | * '''Laura LÓPEZ NAVARRETE''' <br />wrote an essay about crucial factors in biosafety and social implications relevant to achieving success in projects like these. |
* '''Michael TESKE''' <br />created and evaluated our survey about synthetic Biology. | * '''Michael TESKE''' <br />created and evaluated our survey about synthetic Biology. | ||
Line 95: | Line 86: | ||
=== General Project === | === General Project === | ||
− | * '''Balthasar SCHLOTMANN''' <br />gave advice and motivation to the team and represented the team in iGEM meet ups with holding presentations. | + | * '''Balthasar SCHLOTMANN''' <br />gave advice and motivation to the team and represented the team in iGEM meet ups with holding presentations. Balthasar also participated in the development of the project idea. |
− | * '''Sophia MÄDLER''' <br /> | + | * '''Sophia MÄDLER''' <br />was responsible for organizing the labwork and planning experiments throughout all areas. Sophia was involved in the development of the project idea. |
− | * '''Niklas Arndt SCHMACKE''' <br />gave | + | * '''Niklas Arndt SCHMACKE''' <br />gave scientific advice, technical support and planned experiments for the cloning, B. Subtilis and deinking parts of the project. |
− | == | + | == Wiki support == |
− | + | * '''Balthasar SCHLOTMANN''' <br />created graphics for the wiki. | |
+ | * '''Michael TESKE''' <br />designed and programmed the wiki template. Michael organized the creation of the wiki. | ||
+ | |||
+ | |||
+ | === Former Members === | ||
+ | We would also like to thank people who are no longer involved in the project, but whose contribution was essential: Cathleen Hagemann, Mariya Chernyavska, Pavel Rhyzhov, Max Schelski, Guido de Boer, Katrin Ciupka, Alena Sommer, Ashwin Shah, Clarissa Hiltl, Virginia Malchus and Paul Maier. | ||
<html><img src="//2016.igem.org/wiki/images/5/50/T--UBonn_HBRS--monument.svg" alt="Heart" class="col-xs-4 col-md-2" style="float:none"/></html> | <html><img src="//2016.igem.org/wiki/images/5/50/T--UBonn_HBRS--monument.svg" alt="Heart" class="col-xs-4 col-md-2" style="float:none"/></html> | ||
+ | |||
+ | |||
+ | == Human Practices support == | ||
+ | * '''CAESAR''' <br />We thank the Center of Advanced European Studies and Research (caesar) for providing the venue for our science slams, contact to the public and especially Christian Siegel for creating, with a lot of expertise, the movies of the slams. | ||
+ | |||
+ | |||
+ | == Fotos == | ||
+ | Our team's official photos were taken by Kim Hyuntae. | ||
+ | |||
+ | |||
+ | == General support == | ||
+ | We thank the '''<html><a href="https://www.uni-bonn.de/" target="_blank">Rheinische Friedrich-Wilhelms-University of Bonn</a></html>''', the '''<html><a href="http://limes-institut-bonn.de/" target="_blank">Limes Institute Bonn</a></html>''' and the '''<html><a href="https://www.h-brs.de/" target="_blank">University of Applied Sciences Bonn-Rhein-Sieg<html></a></html>''' for providing the laboratory space, material and funding. | ||
{{UBonn_HBRS/footer}} | {{UBonn_HBRS/footer}} |
Revision as of 00:03, 19 October 2016
Attributions
We are very thankful for the lots of help we received! Please also check out our great Sponsors, whom we dedicated a special page:Our Sponsors
Contents
Scientific supervision
We are very grateful to Prof. Dr. Günther Mayer and Prof. Dr. Michael Famulok of University Bonn for supervising this project. We would also like to thank Prof. Dr. Klaus Lehmann of HBRS for his resourceful and unlimited support.
Lab support
Our dearest thanks go to our Instructor Ignacio Geraci and all the other PhDs and PhD students of Professor Mayer’s laboratory, especially Christian Renzl, for their help and patience.
Project advisor support
- Benedikt HÖLBLING
took care of the team’s finances, initiated and organized the team's science slams.
- BÙI Hoàng Duy
gave advices to the project.
- Niklas Arndt SCHMACKE
Was involved in the development of the project idea, concerned himself with the theoretical and practical introduction of new team members and served as a remote advisor from Switzerland for a large part of the project.
Team Attributions
Our Team Members worked in the following groups, learn more about our team on our
Team Page
B. subtilis
- Ariana Natalie KLAPPERT
optimzied PAGE gel analysis and protein expression.
- Jonas GOCKEL
prepared competent bacteria, tested transformation protocols and established the Bacillus Subtilis collaboration with Freiburg.
Cloning
- Caroline FANDREY
planned and executed restriction and ligation of genes, secretion tags and backbones and their transformation into E. coli, and validated the results.
- Ariana Natalie KLAPPERT
supported the cloning group during planning of restrictions, preparation of DNA, ligations and transformations. Ariana also worked on optimizing IPTG inducible protein expression in E. Coli.
- BÙI Hoàng Duy
provided help in all tasks of cloning.
- Jonas GOCKEL
took part in backbone cloning.
- Laura LÓPEZ NAVARRETE
collaborated in assembling DNA constructs of tags and genes in E. coli and prepared rapid ligase buffer.
- Michael TESKE
supported the team with restriction analysis, ligations, minipreps, transformations and prepared rapid ligase buffer.
- Sophia MÄDLER
assembled our working backbone, took care of introducing the working methods to new members, designed the pLac-RBS construct, performed analysis of sequencing results and planned experiments to assemble all constructs.
- Triinu LINKGREIM
took part in backbone cloning.
- Niklas Arndt SCHMACKE
Designed the part sequences of nprb and sacB as well as the genes to be tested in deinking together with Max Schelski.
Deinking
- Gintarė MALININAITĖ
was responsible for the deinking part of the project and took part in the design of the experiments.
- Stefan KOTTE
did a solvent analysis, established the grayscale analysis and refined the deinking process on the lab scale.
Specific Enzyme Essays
- Sophia MÄDLER
established all protocols to determine specific enzymatic activity of lipases, xylanases and endoglucanases in our lab.
- Triinu LINKGREIM
refined the enzyme activity assays and was responsible for measuring enzymatic activity in the project.
Human Practice
- Alina SIEBENMORGEN
one of the main organizing personnel of Science Slam, participated in contacting sponsors and organized team events.
- Ariana Natalie KLAPPERT
worked on and held our science slam presentation.
- Benedikt HÖLBLING
one of the main organizing personnel of Science Slam, participated in contacting sponsors
- Caroline FANDREY
helped with our presentation for the science slam.
- BÙI Hoàng Duy
sponsoring delegate of the team.
- Gintarė MALININAITĖ
responsible for the teams sponsoring undertakings, contacted paper companies and helped with science slam organization.
- Jan Niklas HANSEN
co-organized Science Slam and provided contact with the CAESAR institute.
- Laura LÓPEZ NAVARRETE
wrote an essay about crucial factors in biosafety and social implications relevant to achieving success in projects like these.
- Michael TESKE
created and evaluated our survey about synthetic Biology.
General Project
- Balthasar SCHLOTMANN
gave advice and motivation to the team and represented the team in iGEM meet ups with holding presentations. Balthasar also participated in the development of the project idea.
- Sophia MÄDLER
was responsible for organizing the labwork and planning experiments throughout all areas. Sophia was involved in the development of the project idea.
- Niklas Arndt SCHMACKE
gave scientific advice, technical support and planned experiments for the cloning, B. Subtilis and deinking parts of the project.
Wiki support
- Balthasar SCHLOTMANN
created graphics for the wiki.
- Michael TESKE
designed and programmed the wiki template. Michael organized the creation of the wiki.
Former Members
We would also like to thank people who are no longer involved in the project, but whose contribution was essential: Cathleen Hagemann, Mariya Chernyavska, Pavel Rhyzhov, Max Schelski, Guido de Boer, Katrin Ciupka, Alena Sommer, Ashwin Shah, Clarissa Hiltl, Virginia Malchus and Paul Maier.
Human Practices support
- CAESAR
We thank the Center of Advanced European Studies and Research (caesar) for providing the venue for our science slams, contact to the public and especially Christian Siegel for creating, with a lot of expertise, the movies of the slams.
Fotos
Our team's official photos were taken by Kim Hyuntae.
General support
We thank the Rheinische Friedrich-Wilhelms-University of Bonn, the Limes Institute Bonn and the University of Applied Sciences Bonn-Rhein-Sieg for providing the laboratory space, material and funding.