Difference between revisions of "Team:Wageningen UR/Notebook"
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| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/toggleswitch" style="border:5px solid #fff372"> <!--Change box color here--> |
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| − | <img src="https://static.igem.org/mediawiki/2016/ | + | <img src="https://static.igem.org/mediawiki/2016/9/96/T--Wageningen_UR--togglebutton.jpg"/> |
| − | <h3 style="color:#6d5d31"> | + | <h3 style="color:#6d5d31">Toggle Switch</h3> <!--Change text color here--> |
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| − | <p>We | + | <p font style="font-size:15px; font-family: snap,arial, sans-serif";> We designed a toggle switch that could coordinate mites sensing, BT's toxin production and light sensing with fast responsiveness.</p> |
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| − | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/ | + | <a href="https://2016.igem.org/Team:Wageningen_UR/Notebook/Outreach" style="border:5px solid #fff372"> <!--Change box color here--> |
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| − | <p | + | <p>We implemented our outreach into our research as much as we could. Find the details here!</p> |
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| + | <img src="https://static.igem.org/mediawiki/2016/8/81/T--Wageningen_UR--equation_button2.jpg"/> | ||
| + | <h3 style="color:#6d5d31">Modelling</h3> <!--Change text color here--> | ||
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| + | <p> How we modelled</i>. </p> | ||
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| + | <img src="https://static.igem.org/mediawiki/2016/6/61/T--Wageningen_UR--lightbutton.jpg"/> | ||
| + | <h3 style="color:#6d5d31; font-size:18px;">Kill Switch Model</h3> <!--Change text color here--> | ||
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| + | <p>The optogenetic kill switch was modeled with ODEs to understand the system in more detail.</p> | ||
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| + | <h3 style="color:#6d5d31">Metabolic Modelling</h3> <!--Change text color here--> | ||
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| + | <p>We wanted to use a metabolic model to see the effect of our BeeT distribution method.</p> | ||
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Revision as of 09:49, 19 October 2016
Find the documentation of our research here.
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Protein Engineering
We wanted to engineer Cry3Aa to become toxic to Varroa destructor.
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In Vitro Assay
To provide an alternative for tests on live Varroa mites, we designed an assay that uses brush border membrane vesicles.
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Varroa isolates
We tried to find our own Varroa entomopathogen.
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Riboswitches
To detect the presence of Varroa mites in the beehive, we made a B12 and a guanine riboswitch.
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Population Dynamics
A quorum sensing system was characterized to regulate toxin production in BeeT.
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Optogenetic Kill Switch
The light-induced kill switch was one of our biocontainment methods.
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Chitinases
As a back-up, we wanted to use chitinases which are more harmful to Varroa destructor than honeybees.
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Cas9-based Kill Switch
The Cas9-based kill switch was one of our biocontainment methods.
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Toxin Scanner
We made a software tool for finding new Cry proteins toxic to Varroa destructor.
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Testing E. coli survival
Following the results of the Metabolic Model, we needed more data.
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Toggle Switch
We designed a toggle switch that could coordinate mites sensing, BT's toxin production and light sensing with fast responsiveness.
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Human Outreach
We implemented our outreach into our research as much as we could. Find the details here!
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BEEHAVE
We adapted the BEEHAVE model to include BeeT.
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Modelling
How we modelled.
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Kill Switch Model
The optogenetic kill switch was modeled with ODEs to understand the system in more detail.
Metabolic Modelling
We wanted to use a metabolic model to see the effect of our BeeT distribution method.
