Difference between revisions of "Team:BNU-China/Proof"

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                     </header>
 
                     </header>
  
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                    <h2>The expression of α-tubulin, β-tubulin, n-luciferase, c-luciferase</h2>
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<h2>Vector Construction</h2>
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                    <h3>Vector construction</h3>
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<h3>Vectors of α-tubulin, β-tubulin, n-luciferase, c-luciferase</h3>
−
 
+
<p>Gene fragments of α-tubulin、β-tubulin、n-luciferase、c-luciferase were amplified via PCR and verified by electrophoresis(Fig.1). The theoretic gene size of α-tubulin is 1356bp, β-tubulin is 1335bp, n-luciferase is 1248bp, c-luciferase is 459bp, which matched our experimental results.</p>
−
 
+
<figure class="text-center">
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                    <p>Gene fragments of α-tubulin, β-tubulin, n-luciferase, c-luciferase were amplified via PCR and
+
−
                    verified by electrophoresis (Fig.1). The theoretic gene size of α-tubulin is 1356bp, β-tubulin is
+
−
                    1335bp, n-luciferase is 1248bp, c-luciferase is 459bp, which matched our experimental results.</p>
+
−
                    <figure class="text-center">
+
 
                         <img src="https://static.igem.org/mediawiki/2016/3/3b/T--BNU-China--Results9.jpg" width="65%">
 
                         <img src="https://static.igem.org/mediawiki/2016/3/3b/T--BNU-China--Results9.jpg" width="65%">
 
                         <figcaption>
 
                         <figcaption>
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                             Fig.1    Electrophoresis result of α-tubulin, β-tubulin, n-luciferase, c-luciferase gene fragments
+
                             Fig.1    Electrophoresis result of α-tubulin, β-tubulin, n-luciferase, c-luciferase gene fragments                       </figcaption>
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                        </figcaption>
+
 
                     </figure>
 
                     </figure>
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<h3>Protein expression</h3>
 
−
                    <p>Gene fragments were ligated to <i>E.coli</i> expression plasmid pET30a(+), after transformation, colony PCR
 
−
                    was done to verify the efficiency (Fig.2A and Fig.2B). Meanwhile, the sequencing results further
 
−
                    confirmed that we successfully constructed the α-tubulin, β-tubulin, n-luciferase, c-luciferase
 
−
                    expression vectors. </p>
 
  
−
                    <figure class="text-center">
+
<p>Gene fragments were ligated to <i>E.coli</i> expression plasmid pET30a(+), after transformation, colony PCR was done to verify the efficiency(Fig.2A and 2B). Meanwhile, the sequencing results further confirmed that we successfully cloned the α-tubulin, β-tubulin, n-luciferase, c-luciferase expression vectors.</p>
 +
 
 +
<figure class="text-center">
 
                         <img src="https://static.igem.org/mediawiki/2016/c/c0/T--BNU-China--Results5.jpg" width="88%">
 
                         <img src="https://static.igem.org/mediawiki/2016/c/c0/T--BNU-China--Results5.jpg" width="88%">
 
                         <figcaption>
 
                         <figcaption>
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                     </figure>
 
                     </figure>
  
 +
 +
<h3>Fusion Protein Vectors</h3>
 +
<h4>By fusion PCR technology</h4>
 +
<p>
 +
α-tubulin-YNE, YNE-α-tubulin, α-tubulin-YCE, YCE-α-tubulin, β-tubulin-YNE, YNE-β-tubulin, β-tubulin-YCE, YCE-β-tubulin, α-tubulin-nluc, nluc-α-tubulin, α-tubulin-cluc and cluc-α-tubulin were cloned respectively via fusion PCR. After ligating these fusion gene fragments to pET30a(+) empty vectors, we transformed the target plasmids to Trans5α. When colony PCR was done for screening, we picked correct colonies shown in SDS-PAGE (Fig.3) for plasmid amplification.
 +
</p>
 +
 +
 +
                    <figure class="text-center">
 +
                        <img src="https://static.igem.org/mediawiki/2016/b/b9/T--BNU-China--Results6.jpg" width="75%">
 +
                        <figcaption>
 +
                            Fig.3    Result of colony PCR
 +
                            <br/>
 +
                            <small style="font-size:80%">Arrows show the correct size of fusion gene fragments:
 +
                                α-tubulin-YNE is 1866 bp, α-tubulin-YCE is 1650bp, β-tubulin-YCE is 1629bp,
 +
                                α-tubulin-nluc is 2640bp, α-tubulin-cluc is 1857bp.
 +
                            </small>
 +
                        </figcaption>
 +
                    </figure>
 +
 +
<P>Sequencing results further confirmed that α-tubulin-YNE, YNE-α-tubulin, α-tubulin-YCE, YCE-α-tubulin, β-tubulin-YCE, YCE-β-tubulin, α-tubulin-nluc and cluc-α-tubulin expression vectors were constructed successfully.</P>
 +
 +
<h4>By Gateway Technology</h4>
 +
<p>We also tried to construct fusion protein vectors by Gateway Large-scale Cloning technology. We used Invitrogen pENTR/D TOPO to clone β-tubulin into entry vector. Primers were designed based on β-tubulin sequence and PCR was done for verification.  Electrophoresis result (Fig.4) showed that β-tubulin was successfully cloned into the entry vector.</p>
 +
 +
                    <figure class="text-center">
 +
                        <img src="https://static.igem.org/mediawiki/2016/6/63/T--BNU-China--Results14.jpg" width="65%">
 +
                        <figcaption>
 +
                            Fig.4    PCR verification result of the constructed entry vectors
 +
                        </figcaption>
 +
                    </figure>
 +
<p>In order to do LR reaction, we used the restriction endonuclease Not I to digest the entry vector. Electrophoresis result (Fig.5) showed that single digestion was efficient.</p>
 +
<figure class="text-center">
 +
                        <img src="https://static.igem.org/mediawiki/2016/2/2e/T--BNU-China--Results4.jpg" width="25%">
 +
                        <figcaption>
 +
                            Fig.5    Single endonuclease digestion result of entry vectors
 +
                        </figcaption>
 +
                    </figure>
 +
<p>Using Invitrogen Gateway LR Clonase II Enzyme Mix, the entry vectors were ligated with pCambia1300-nluc and pCambia1300-cluc respectively. Thus the destination vectors were complete. After transformation and running PCR with β-tubulins primers, electrophoresis result (Fig.6) showed high positive rates, indicating β-tubulins was successfully cloned into the vectors.</p>
 +
<figure class="text-center">
 +
                        <img src="https://static.igem.org/mediawiki/2016/d/d3/T--BNU-China--Results10.jpg" width="90%">
 +
                        <figcaption>
 +
                            Fig.6  PCR verification result of the objective vectors
 +
                        </figcaption>
 +
                    </figure>
 +
 +
<p>Also, signaling fragments were also need to be tested. By using the reverse primer of β-tubulin and the forward primer of cluc for PCR verification, we found that cluc-β-tubulin  fusion protein vector is successfully constructed. Electrophoresis result is shown in Fig.7.</p>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
                    <h2>The expression of α-tubulin, β-tubulin, n-luciferase, c-luciferase</h2>
 +
                    <h3>Vector construction</h3>
 +
 +
 +
                    <p>Gene fragments of α-tubulin, β-tubulin, n-luciferase, c-luciferase were amplified via PCR and
 +
                    verified by electrophoresis (Fig.1). The theoretic gene size of α-tubulin is 1356bp, β-tubulin is
 +
                    1335bp, n-luciferase is 1248bp, c-luciferase is 459bp, which matched our experimental results.</p>
 +
                    <h3>Protein expression</h3>
 +
                    <p>Gene fragments were ligated to <i>E.coli</i> expression plasmid pET30a(+), after transformation, colony PCR
 +
                    was done to verify the efficiency (Fig.2A and Fig.2B). Meanwhile, the sequencing results further
 +
                    confirmed that we successfully constructed the α-tubulin, β-tubulin, n-luciferase, c-luciferase
 +
                    expression vectors. </p>
 +
 +
                   
 
                     <p>Expression vectors were transformed to <i>E.coli</i> expression strain TransB(DE3). After culturing
 
                     <p>Expression vectors were transformed to <i>E.coli</i> expression strain TransB(DE3). After culturing
 
                     and inducing with IPTG, bacteria were lysed and SDS-PAGE(Fig.3) / western-blot (Fig.4) were done to
 
                     and inducing with IPTG, bacteria were lysed and SDS-PAGE(Fig.3) / western-blot (Fig.4) were done to
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                     pET30a(+) with restriction enzyme, we transformed the target plasmids to Trans5α. When colony
 
                     pET30a(+) with restriction enzyme, we transformed the target plasmids to Trans5α. When colony
 
                     PCR was done, we picked correct colony shown in electrophoresis(Fig.8) for plasmid amplification.</p>
 
                     PCR was done, we picked correct colony shown in electrophoresis(Fig.8) for plasmid amplification.</p>
−
 
−
                    <figure class="text-center">
 
−
                        <img src="https://static.igem.org/mediawiki/2016/b/b9/T--BNU-China--Results6.jpg" width="75%">
 
−
                        <figcaption>
 
−
                            Fig.8    Result of colony PCR
 
−
                            <br/>
 
−
                            <small style="font-size:80%">arrows show the correct size of fusion gene fragments:
 
−
                                α-tubulin-YNE is 1866 bp, α-tubulin-YCE is 1650bp, β-tubulin-YCE is 1629bp,
 
−
                                α-tubulin-nluc is 2640bp, α-tubulin-cluc is 1857bp
 
−
                            </small>
 
−
                        </figcaption>
 
−
                    </figure>
 
−
 
 
                     <p>Sequencing results showed that α-tubulin-YNE, YNE-α-tubulin, α-tubulin-YCE, YCE-α-tubulin,
 
                     <p>Sequencing results showed that α-tubulin-YNE, YNE-α-tubulin, α-tubulin-YCE, YCE-α-tubulin,
 
                     β-tubulin-YCE, YCE-β-tubulin, α-tubulin-nluc, cluc-α-tubulin expression vectors were constructed
 
                     β-tubulin-YCE, YCE-β-tubulin, α-tubulin-nluc, cluc-α-tubulin expression vectors were constructed
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                     Designing primer based on β-tubulin sequence and running PCR procedure. From this picture, the
 
                     Designing primer based on β-tubulin sequence and running PCR procedure. From this picture, the
 
                     band of β-tubulin was correct.</p>
 
                     band of β-tubulin was correct.</p>
−
 
−
                    <figure class="text-center">
 
−
                        <img src="https://static.igem.org/mediawiki/2016/6/63/T--BNU-China--Results14.jpg" width="65%">
 
−
                        <figcaption>
 
−
                            Fig.11    PCR verification result of the constructed entry vectors
 
−
                        </figcaption>
 
−
                    </figure>
 
  
 
                     <p>In order to do LR reaction, we used the restriction endonuclease Not I to digest the entry
 
                     <p>In order to do LR reaction, we used the restriction endonuclease Not I to digest the entry
 
                     vector. The bands of linear vector and the origin vector suggested that the digestion was
 
                     vector. The bands of linear vector and the origin vector suggested that the digestion was
 
                     efficiency.</p>
 
                     efficiency.</p>
−
                     <figure class="text-center">
+
                      
−
                        <img src="https://static.igem.org/mediawiki/2016/2/2e/T--BNU-China--Results4.jpg" width="25%">
+
−
                        <figcaption>
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                            Fig.12    Single endonuclease digestion result of entry vectors
+
−
                        </figcaption>
+
−
                    </figure>
+
−
 
+
 
                     <p>Using Invitrogen Gateway LR Clonase II Enzyme Mix, the entry vector can be ligate with
 
                     <p>Using Invitrogen Gateway LR Clonase II Enzyme Mix, the entry vector can be ligate with
 
                     pCambia1300-nluc and pCambia1300-cluc respectively. So the destination vectors were complete.
 
                     pCambia1300-nluc and pCambia1300-cluc respectively. So the destination vectors were complete.
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                     as showed in Fig.13</p>
 
                     as showed in Fig.13</p>
  
−
                     <figure class="text-center">
+
                      
−
                        <img src="https://static.igem.org/mediawiki/2016/d/d3/T--BNU-China--Results10.jpg" width="90%">
+
−
                        <figcaption>
+
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                            Fig.13  PCR verification result of the objective vectors
+
−
                        </figcaption>
+
−
                    </figure>
+
−
 
+
 
                     <p>Extracting plasmid, the product gal bands show that cluc-β-tubulin is correct. Running PCR using
 
                     <p>Extracting plasmid, the product gal bands show that cluc-β-tubulin is correct. Running PCR using
 
                     the reverse primer of β-tubulin and the forward primer of cluc, the correct band existed.</p>
 
                     the reverse primer of β-tubulin and the forward primer of cluc, the correct band existed.</p>

Revision as of 11:52, 19 October 2016

Team:BNU-CHINA - 2016.igem.org

RESULTS

Results

Do One Thing

Vector Construction

Vectors of α-tubulin, β-tubulin, n-luciferase, c-luciferase

Gene fragments of α-tubulin、β-tubulin、n-luciferase、c-luciferase were amplified via PCR and verified by electrophoresis(Fig.1). The theoretic gene size of α-tubulin is 1356bp, β-tubulin is 1335bp, n-luciferase is 1248bp, c-luciferase is 459bp, which matched our experimental results.

Fig.1 Electrophoresis result of α-tubulin, β-tubulin, n-luciferase, c-luciferase gene fragments

Gene fragments were ligated to E.coli expression plasmid pET30a(+), after transformation, colony PCR was done to verify the efficiency(Fig.2A and 2B). Meanwhile, the sequencing results further confirmed that we successfully cloned the α-tubulin, β-tubulin, n-luciferase, c-luciferase expression vectors.

Fig.2 Electrophoresis result of α-tubulin、β-tubulin、n-luciferase、c-luciferase expression vectors
（A: electrophoresis result of colony PCR. The arrows show the correct sizes of α-tubulin, n-luciferase and c-luciferase.
B: electrophoresis result of colony PCR. The arrows show the correct size of β-tubulin.

Fusion Protein Vectors

By fusion PCR technology

α-tubulin-YNE, YNE-α-tubulin, α-tubulin-YCE, YCE-α-tubulin, β-tubulin-YNE, YNE-β-tubulin, β-tubulin-YCE, YCE-β-tubulin, α-tubulin-nluc, nluc-α-tubulin, α-tubulin-cluc and cluc-α-tubulin were cloned respectively via fusion PCR. After ligating these fusion gene fragments to pET30a(+) empty vectors, we transformed the target plasmids to Trans5α. When colony PCR was done for screening, we picked correct colonies shown in SDS-PAGE (Fig.3) for plasmid amplification.

Fig.3 Result of colony PCR
Arrows show the correct size of fusion gene fragments: α-tubulin-YNE is 1866 bp, α-tubulin-YCE is 1650bp, β-tubulin-YCE is 1629bp, α-tubulin-nluc is 2640bp, α-tubulin-cluc is 1857bp.

Sequencing results further confirmed that α-tubulin-YNE, YNE-α-tubulin, α-tubulin-YCE, YCE-α-tubulin, β-tubulin-YCE, YCE-β-tubulin, α-tubulin-nluc and cluc-α-tubulin expression vectors were constructed successfully.

By Gateway Technology

We also tried to construct fusion protein vectors by Gateway Large-scale Cloning technology. We used Invitrogen pENTR/D TOPO to clone β-tubulin into entry vector. Primers were designed based on β-tubulin sequence and PCR was done for verification. Electrophoresis result (Fig.4) showed that β-tubulin was successfully cloned into the entry vector.

Fig.4 PCR verification result of the constructed entry vectors

In order to do LR reaction, we used the restriction endonuclease Not I to digest the entry vector. Electrophoresis result (Fig.5) showed that single digestion was efficient.

Fig.5 Single endonuclease digestion result of entry vectors

Using Invitrogen Gateway LR Clonase II Enzyme Mix, the entry vectors were ligated with pCambia1300-nluc and pCambia1300-cluc respectively. Thus the destination vectors were complete. After transformation and running PCR with β-tubulins primers, electrophoresis result (Fig.6) showed high positive rates, indicating β-tubulins was successfully cloned into the vectors.

Fig.6 PCR verification result of the objective vectors

Also, signaling fragments were also need to be tested. By using the reverse primer of β-tubulin and the forward primer of cluc for PCR verification, we found that cluc-β-tubulin fusion protein vector is successfully constructed. Electrophoresis result is shown in Fig.7.

The expression of α-tubulin, β-tubulin, n-luciferase, c-luciferase

Vector construction

Gene fragments of α-tubulin, β-tubulin, n-luciferase, c-luciferase were amplified via PCR and verified by electrophoresis (Fig.1). The theoretic gene size of α-tubulin is 1356bp, β-tubulin is 1335bp, n-luciferase is 1248bp, c-luciferase is 459bp, which matched our experimental results.

Protein expression

Gene fragments were ligated to E.coli expression plasmid pET30a(+), after transformation, colony PCR was done to verify the efficiency (Fig.2A and Fig.2B). Meanwhile, the sequencing results further confirmed that we successfully constructed the α-tubulin, β-tubulin, n-luciferase, c-luciferase expression vectors.

Expression vectors were transformed to E.coli expression strain TransB(DE3). After culturing and inducing with IPTG, bacteria were lysed and SDS-PAGE(Fig.3) / western-blot (Fig.4) were done to test the protein from supernatant, pellet and renatured inclusion body.

Fig.3 SDS-PAGE result of β-tubulin
(left to right: non-induced group, expressed empty vector, induced group) arrow shows the correct molecular weight of target protein(55 kDa).
Fig.4 Western blot result of prokaryotic expression
(left to right, extracted α-tubulin, expressed empty vector, α-tubulin, α-tubulin-YNE fusion protein, α-tubulin-YCE fusion protein, α-tubulin-nluc fusion protein) arrows show the correct bands of target proteins, triangles show the homologous tubulin protein(FtsZ,43kDa) from the bacteria.

Apart from this, we also transformed plasmids to Rossatta(DE3) which can express rare codons and improve the expression level of eukaryotic protein.

Before breaking the bacteria via ultrasonic waves, SDS-PAGE (Fig.5) was established to verify the results.

Fig.5 SDS-PAGE of rossatta cells before ultrasonic breaking
(left to right: protein marker, expressed empty vector, α-tubulin(55 kDa), β-tubulin(55 kDa), α-tubulin-YNE(75kDa), YNE-α-tubulin(75 kDa) ) arrows show the correct band.

After breaking the bacteria, SDS-PAGE (Fig.6) was also established to verify the results.

Fig.6 SDS-PAGE of supernatant after ultrasonic breaking the rossatta cells
left to right: expressed empty vector, α-tubulin(55 kDa), β-tubulin(55 kDa), α-tubulin-YNE(75kDa), YNE-α-tubulin(75kDa), α-tubulin-YCE(66 kDa), YCE-α-tubulin(66 kDa), β-tubulin-YCE(66 kDa), YCE-β-tubulin(66 kDa), α-tubulin-nluc, cluc-α-tubulin(74 kDa).

Western blot(Fig.7) was also applied for the further confirmation.

Fig.7 Western blot of rossatta cells expression
A: left to right, protein marker, negative control, α-tubulin(55 kDa), α-tubulin-YNE(75kDa), α-tubulin-YCE(66 kDa), YCE-α-tubulin(66 kDa), cluc-α-tubulin(74 kDa), extracted α-tubulin(55 kDa).
B: left to right: negative control in pellet, β-tubulin in pellet(55 kDa), β-tubulin-YCE in pellet (66 kDa), protein marker, negative control in supernatant, β-tubulin in supernatant(55 kDa), β-tubulin-YCE in supernatant(66 kDa), extracted β-tubulin(55 kDa).

According to Fig.7B, the target protein can be tested out in the supernatant, indicating that they are soluble when expressed in rossatta strain.

Based on the results above, we can confirm that α-tubulin and β-tubulin were successfully expressed in cell.

We collaborated with Fujian Agriculture and Forest University and asked them to test the interaction between α and β-tubulin. Thus verified the activity of tubulin monomers.

The expression of fusion protein

Fusion PCR

α-tubulin-YNE, YNE-α-tubulin, α-tubulin-YCE, YCE-α-tubulin, β-tubulin-YNE, YNE-β-tubulin, β-tubulin-YCE, YCE-β-tubulin, α-tubulin-nluc, nluc-α-tubulin, α-tubulin-cluc, cluc-α-tubulin were constructed respectively via fusion PCR. After ligating these fusion gene fragments to pET30a(+) with restriction enzyme, we transformed the target plasmids to Trans5α. When colony PCR was done, we picked correct colony shown in electrophoresis(Fig.8) for plasmid amplification.

Sequencing results showed that α-tubulin-YNE, YNE-α-tubulin, α-tubulin-YCE, YCE-α-tubulin, β-tubulin-YCE, YCE-β-tubulin, α-tubulin-nluc, cluc-α-tubulin expression vectors were constructed successfully.

We transformed the expression plasmids to E.coli expression strain TranB(DE3). The protein expression predicted website http://www.biotech.ou.edu/ showed that our fusion protein would probably expressed as inclusion bodies. We therefore renatured the inclusion bodies and verified through SDS-PAGE(Fig.9).

Fig.9 SDS-PAGE of renatured inclusion bodies from α-tubulin-YNE, YNE-α-tubulin
the molecular weight of target fusion protein is 74.6kDa. arrows show the correct bands.

Also, western-blot (Fig.4) were done to test the protein from supernatant, pellet and renatured inclusion body.

Apart from these, we also expressed our target protein through Rossatta(DE3) strain and used SDS-PAGE(Fig.10) to verify the expression.

Fig.10 SDS-PAGE of rossatta cells before ultrasonic breaking
A: cluc-α-tubulin(74 kDa), α-tubulin-nluc, YCE-β-tubulin(66 kDa), β-tubulin-YCE(66 kDa), YCE-α-tubulin(66 kDa), α-tubulin-YCE(66 kDa), expressed empty vector.
B: left to right: expressed empty vector, α-tubulin(55 kDa), β-tubulin(55 kDa), α-tubulin-YNE(75kDa), YNE-α-tubulin(75kDa).

SDS-PAGE were done to verify the expression results before(Fig.5) and after(Fig.6) breaking the bacteria, and Western blot(Fig.7) was also applied for the further confirmation.

Based on the results above, we can confirm that α-tubulin-YNE, YNE-α-tubulin, α-tubulin-YCE, YCE-α-tubulin, β-tubulin-YCE, YCE-β-tubulin, cluc-α-tubulin fusion protein were successfully expressed in cell.

Gateway

In our experiment, we also try to construct fusion protein vectors with Gateway Large-scale Cloning technology. We used Invitrogen pENTR/D TOPO to clone β-tubulin into entry vector. Designing primer based on β-tubulin sequence and running PCR procedure. From this picture, the band of β-tubulin was correct.

In order to do LR reaction, we used the restriction endonuclease Not I to digest the entry vector. The bands of linear vector and the origin vector suggested that the digestion was efficiency.

Using Invitrogen Gateway LR Clonase II Enzyme Mix, the entry vector can be ligate with pCambia1300-nluc and pCambia1300-cluc respectively. So the destination vectors were complete. After transformation, running PCR with β-tubulin's primers, the bands show high positive rates as showed in Fig.13

Extracting plasmid, the product gal bands show that cluc-β-tubulin is correct. Running PCR using the reverse primer of β-tubulin and the forward primer of cluc, the correct band existed.

Fig.14 PCR verification result of pCambia-nluc, pCambia-cluc
arrows show the correct bands

Results of tublin extraction in vitro

After successfully extracting tubulin from porcine brains, we tried to summarize the aggregation conditionin vitro by using electron microscope.

Fig.15 Aggregated microtubule treated with 1μM taxol

From pictures taken under the electron microscope(Fig.15), we could see tubulin (treated with 1μM taxol) in aggregated form obviously, indicating we have achieved the aggregation process in vitro. However, due to the high concentration of our extracted sample, it was hard to tell the aggregated length and the quantity of microtubules. Thus we tried to use spectrophotometer to measure OD350 of our experimental samples.

Table 1 OD350 of microtubule samples treated with serial concentration of taxol

Taxol concentraion(μM) 1 2 3 4 5
0 0.095 0.077 0.025 0.104
0.001 0.062 0.123 0.119 0.086 0.149
0.01 0.152 0.138 0.129 0.060 0.081
0.1 0.096 0.106 0.123 0.082 0.134
1 0.148 0.140 0.149 0.061 0.092
2 0.047 0.093 0.108 0.052 0.080
3 0.068 0.091
4 0.035 0.050
5 0.020 0.059 0.140 0.078 0.100
6 0.079 0.112
7 0.076 0.076
8 0.050 0.067
9 0.079 0.107
10 0.053 0.111 0.185 0.086 0.100
12.5 0.077 0.065
20 0.028 0.099 0.108
25 0.076 0.097
30 0.093 0.164 0.154
50 0.043 0.162 0.096 0.074 0.090
70 0.113 0.188 0.156
100 0.072 0.088

From the results shown in Table 1, we found that there was no obvious relationship between OD statistics and taxol concentration. The reason may be the machine issue. Due to the wave length for measuring OD is 350nm, which is between the ultraviolet light and visible light, there is a high requirement for instruments and always leads to a huge deviation. As the high technologic instruments could not be owned by every laboratory in different areas, our fusion proteins which can detect the relatively accurate concentration of anti-microtubule drugs will have a broad application prospect.