Difference between revisions of "Team:Wageningen UR/Notebook"
| Line 79: | Line 79: | ||
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/7/76/T--Wageningen_UR--casbutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/7/76/T--Wageningen_UR--casbutton.jpg"/> | ||
| − | <h3 style="color:#6d5d31">Cas9-based Kill Switch</h3> <!--Change text color here--> | + | <h3 style="color:#6d5d31; font-size:18px;">Cas9-based Kill Switch</h3> <!--Change text color here--> |
</div> | </div> | ||
<p>The Cas9-based kill switch was one of our biocontainment methods.</p> | <p>The Cas9-based kill switch was one of our biocontainment methods.</p> | ||
| Line 101: | Line 101: | ||
<div class="square-menu-header"> | <div class="square-menu-header"> | ||
<img src="https://static.igem.org/mediawiki/2016/5/53/T--Wageningen_UR--metabutton.jpg"/> | <img src="https://static.igem.org/mediawiki/2016/5/53/T--Wageningen_UR--metabutton.jpg"/> | ||
| − | <h3 style="color:#6d5d31"> | + | <h3 style="color:#6d5d31;"><i>E. coli</i> survival</h3> <!--Change text color here--> |
</div> | </div> | ||
<p>Following the results of the Metabolic Model, we needed more data.</p> | <p>Following the results of the Metabolic Model, we needed more data.</p> | ||
| Line 131: | Line 131: | ||
<h3 style="color:#6d5d31">BEEHAVE</h3> <!--Change text color here--> | <h3 style="color:#6d5d31">BEEHAVE</h3> <!--Change text color here--> | ||
</div> | </div> | ||
| − | <p font style="font-size: | + | <p font style="font-size:18px; font-family: snap,arial, sans-serif";> We adapted the BEEHAVE model to include BeeT. </p> |
</a> | </a> | ||
</li> | </li> | ||
Revision as of 17:32, 19 October 2016
Find the documentation of our research here.
-
Protein Engineering
We wanted to engineer Cry3Aa to become toxic to Varroa destructor.
-
In Vitro Assay
To provide an alternative for tests on live Varroa mites, we designed an assay that uses brush border membrane vesicles.
-
Varroa isolates
We tried to find our own Varroa entomopathogen.
-
Riboswitches
To detect the presence of Varroa mites in the beehive, we made a B12 and a guanine riboswitch.
-
Population Dynamics
A quorum sensing system was characterized to regulate toxin production in BeeT.
-
Optogenetic Kill Switch
The light-induced kill switch was one of our biocontainment methods.
-
Chitinases
As a back-up, we wanted to use chitinases which are more harmful to Varroa destructor than honeybees.
-
Cas9-based Kill Switch
The Cas9-based kill switch was one of our biocontainment methods.
-
Toxin Scanner
We made a software tool for finding new Cry proteins toxic to Varroa destructor.
-
E. coli survival
Following the results of the Metabolic Model, we needed more data.
-
Toggle Switch
We designed a toggle switch that could coordinate mites sensing, BT's toxin production and light sensing with fast responsiveness.
-
Human Outreach
We implemented our outreach into our research as much as we could. Find the details here!
-
BEEHAVE
We adapted the BEEHAVE model to include BeeT.
-
Population Model
Modelling of the population dynamics regulation system..
-
Kill Switch Model
The optogenetic kill switch was modeled with ODEs to understand the system in more detail.
-
Metabolic Modelling
We wanted to use a metabolic model to see the effect of our BeeT distribution method.
